##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662849 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.384728648606245 31.0 28.0 31.0 22.0 34.0 2 28.808469198867314 31.0 28.0 33.0 23.0 34.0 3 29.206493484941518 31.0 29.0 33.0 25.0 34.0 4 32.929054731922356 35.0 33.0 37.0 27.0 37.0 5 32.71223159422433 35.0 33.0 37.0 26.0 37.0 6 32.85025850533078 35.0 33.0 37.0 26.0 37.0 7 32.67566821402763 35.0 33.0 37.0 25.0 37.0 8 32.6560136622368 35.0 33.0 37.0 25.0 37.0 9 33.85633681275826 38.0 34.0 39.0 24.0 39.0 10 33.75560648051064 37.0 33.0 39.0 23.0 39.0 11 34.01568833927486 37.0 34.0 39.0 25.0 39.0 12 33.818641953144684 37.0 34.0 39.0 25.0 39.0 13 33.953259339608266 38.0 34.0 39.0 25.0 39.0 14 34.60627080979228 39.0 34.0 40.0 23.0 41.0 15 34.73396806814222 39.0 34.0 40.0 25.0 41.0 16 34.67180609761801 38.0 34.0 40.0 24.0 41.0 17 34.63151336126327 38.0 34.0 40.0 24.0 41.0 18 34.59006953318177 39.0 34.0 40.0 24.0 41.0 19 34.59640129199863 39.0 34.0 40.0 23.0 41.0 20 34.61434353827192 39.0 34.0 40.0 23.0 41.0 21 34.52389458232569 39.0 34.0 40.0 22.0 41.0 22 34.480175726296636 39.0 34.0 40.0 21.0 41.0 23 34.152113075527005 39.0 34.0 40.0 18.0 41.0 24 34.2318220288482 39.0 34.0 41.0 18.0 41.0 25 34.02787361827505 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6037920192875568 1101 2 0.6017969330512898 1101 3 0.7301395136994451 1101 4 0.42536161580847676 1101 5 0.5543191706507216 1101 6 0.2541906452921623 1101 7 0.48902607258267494 1101 8 0.5514367082317762 1101 9 0.6946826390462917 1101 10 0.6091230892784196 1101 11 0.31959175488120906 1101 12 0.4463674334424539 1101 13 0.30023217258765555 1101 14 0.4646101251105321 1101 15 0.5471225591584385 1101 16 0.5743024753060268 1101 17 0.42711192461398895 1101 18 0.6978047900167681 1101 19 0.5513156492138123 1101 20 0.4442205168658546 1101 21 0.6716911412163498 1101 22 0.5367482634996961 1101 23 0.8473206232474695 1101 24 0.7880152692507636 1101 25 0.9056512246139619 1102 1 0.4396940589477438 1102 2 0.43640376907668 1102 3 0.5950317285433471 1102 4 0.30704807601922823 1102 5 0.38605691361561867 1102 6 0.07625205210717212 1102 7 0.4807226065994712 1102 8 0.5195705870582827 1102 9 0.9145131889481775 1102 10 0.7496195611619925 1102 11 0.4783649891668773 1102 12 0.6899273931472294 1102 13 0.5943210642177164 1102 14 0.9817641492648193 1102 15 0.6829042156941938 1102 16 0.6499862263253959 1102 17 0.705729186229135 1102 18 0.9727785775622522 1102 19 0.944445359174999 1102 20 0.7793995724865184 1102 21 0.9809912515485166 1102 22 0.950603515927348 1102 23 1.1127619861580413 1102 24 1.0383550603277882 1102 25 0.864752942643328 1103 1 0.3153962505768284 1103 2 0.19940230231020095 1103 3 0.2914392157471788 1103 4 0.26861012145855767 1103 5 0.38192212325367336 1103 6 0.17720179861827745 1103 7 0.3997864626737737 1103 8 0.36166191609478204 1103 9 0.6104147634398345 1103 10 0.6342440750293292 1103 11 0.31062183154121215 1103 12 0.41473141853872164 1103 13 0.4338143603036997 1103 14 0.7592211352727247 1103 15 0.7322934687387956 1103 16 0.754220981996184 1103 17 0.6718862884828454 1103 18 0.5455531702454621 1103 19 0.5521469482734531 1103 20 0.43025209246436447 1103 21 0.3936296357926423 1103 22 0.27720859057419744 1103 23 0.6123253036064753 1103 24 0.40174493416152757 1103 25 0.6444344230428243 1104 1 0.2330751674046816 1104 2 0.4921265992898185 1104 3 0.33955184779598113 1104 4 0.05484356886221775 1104 5 0.037628863322119344 1104 6 -0.35807122622921383 1104 7 -0.10321532277482248 1104 8 -0.03943613479050612 1104 9 0.046275498449503516 1104 10 0.30327546916710446 1104 11 0.07502073051457359 1104 12 0.275191915488584 1104 13 0.01953151548894283 1104 14 0.4391802187424432 1104 15 0.36051545951398367 1104 16 0.07341455438124456 1104 17 0.1417501480275547 1104 18 0.1993985801546998 1104 19 0.33806645429934434 1104 20 0.23285363985313978 1104 21 0.36422255351321553 1104 22 0.3263411601323014 1104 23 0.36756857097740436 1104 24 -0.11205658346977998 1104 25 0.19022597191948165 1105 1 0.7175270539052363 1105 2 0.6361631462566741 1105 3 0.7004546015589703 1105 4 0.399342413118994 1105 5 0.6186666661359439 1105 6 0.38938632068303747 1105 7 0.21841618624361558 1105 8 0.5038221510693859 1105 9 0.7823954773367134 1105 10 0.6606188978113394 1105 11 0.302653003961602 1105 12 0.45237117399285154 1105 13 0.492078700753666 1105 14 0.8879718755504058 1105 15 0.7735755399609872 1105 16 0.703041622433588 1105 17 0.6175042932325852 1105 18 0.4441074602668138 1105 19 0.8145544509504177 1105 20 0.7688767911189984 1105 21 0.5428105447040892 1105 22 0.6524471244953673 1105 23 1.0836758093881187 1105 24 0.7382078724208725 1105 25 0.8554950689837142 1106 1 0.42633103482831913 1106 2 0.42609663873022185 1106 3 0.39482076769481367 1106 4 0.2886844984531365 1106 5 0.26144025682418004 1106 6 -0.6622802073225813 1106 7 -0.5944376216375957 1106 8 -0.5625630692274619 1106 9 -0.6321228319655958 1106 10 -0.5311059531635891 1106 11 -0.66934884200802 1106 12 -0.5083258857529955 1106 13 -0.7861507327910715 1106 14 -0.3673934180874667 1106 15 -0.4386370139123841 1106 16 -0.4642625295047864 1106 17 -0.32574565148076573 1106 18 -0.5553549615230224 1106 19 -0.4029151342823951 1106 20 -0.19234860276725385 1106 21 -0.3334142056526943 1106 22 -0.664251860861313 1106 23 -0.3322959521120339 1106 24 -0.5291882224574138 1106 25 -0.289628293127123 1107 1 0.7001474790780549 1107 2 0.6331163922742924 1107 3 0.8384175752255381 1107 4 0.6599291065275423 1107 5 0.5305766781582264 1107 6 0.4483757260422081 1107 7 0.6793177422050505 1107 8 0.8755947737776424 1107 9 0.8260167290417968 1107 10 0.7833415991268566 1107 11 0.6027629674323478 1107 12 0.6728407078980112 1107 13 0.6739981629875018 1107 14 0.9075449398465238 1107 15 1.0051093353046667 1107 16 0.9281011558763552 1107 17 0.844254460851019 1107 18 0.9138436635359568 1107 19 0.993875827502336 1107 20 0.6704240826363517 1107 21 0.7990586662216685 1107 22 0.8049502745658828 1107 23 0.9493303593615323 1107 24 0.953873548790142 1107 25 0.9883261731845749 1108 1 1.2161001595179677 1108 2 1.1643163009968518 1108 3 0.969848738678941 1108 4 0.9509107285944225 1108 5 -0.3754813052782566 1108 6 0.3445184193945394 1108 7 0.689686636634903 1108 8 0.8222211262421268 1108 9 1.2551653999532348 1108 10 1.3953067355977566 1108 11 1.0958907224526016 1108 12 0.9903512270662773 1108 13 0.9914015424790179 1108 14 1.3208578099654105 1108 15 0.8068605820209029 1108 16 1.018042827366628 1108 17 0.9415706815197922 1108 18 1.0730226118417576 1108 19 1.2296693846509186 1108 20 1.2320530509786636 1108 21 1.2266600732300006 1108 22 0.973444809050946 1108 23 1.3487540313861146 1108 24 1.13939772618005 1108 25 1.276266141571412 1109 1 0.5414993400339547 1109 2 -0.38717261345725973 1109 3 0.4701308657121217 1109 4 0.8070646793230551 1109 5 -0.5942562015271378 1109 6 0.22331562268241356 1109 7 0.615416329701155 1109 8 0.837716916994502 1109 9 1.2665009133148004 1109 10 -1.0632944113290321 1109 11 0.333066002277306 1109 12 0.7986883001664111 1109 13 0.8174563394425718 1109 14 1.3061718553600912 1109 15 1.2557575822650264 1109 16 1.43301734091677 1109 17 1.2937046874751559 1109 18 1.3329544335784007 1109 19 1.0737503448768493 1109 20 1.2930918924159656 1109 21 0.8869864342736662 1109 22 1.2036641226197915 1109 23 1.2806430107440647 1109 24 1.2390287683397716 1109 25 1.2839230400159138 1110 1 0.7385772727070616 1110 2 -0.39878147538320974 1110 3 0.24841722022956247 1110 4 0.5132107485022672 1110 5 1.0256170885335791 1110 6 0.6885822745346601 1110 7 0.9698405329467548 1110 8 1.108444030652727 1110 9 1.5505516843123033 1110 10 1.0217864800848702 1110 11 1.084269156195539 1110 12 1.1295474693880507 1110 13 1.0657155151908455 1110 14 1.5442642445277386 1110 15 1.4877219281952776 1110 16 0.8450320973256851 1110 17 1.073327448023825 1110 18 1.2706382865302288 1110 19 1.5583638049719255 1110 20 1.233169621520247 1110 21 1.2926214058439598 1110 22 1.5480559568681471 1110 23 1.866440277949124 1110 24 1.701231787063442 1110 25 1.8599699820547428 1111 1 0.39735453202141 1111 2 0.45668868031575727 1111 3 0.49141646452579835 1111 4 0.474939750733391 1111 5 0.7593229758257607 1111 6 0.3792925161199534 1111 7 0.3980561724160836 1111 8 0.3934238185758616 1111 9 0.5490822603265073 1111 10 0.677154075987275 1111 11 0.23008849501471929 1111 12 0.26675268494355464 1111 13 0.08743300193604142 1111 14 0.30611259997240836 1111 15 0.4417184615542027 1111 16 0.42162207405480956 1111 17 0.12449788921087901 1111 18 0.45945177164406203 1111 19 0.21379437618800523 1111 20 0.05937724813061607 1111 21 0.3647056488207099 1111 22 0.3827802449472202 1111 23 0.860277693682157 1111 24 0.5865327490863308 1111 25 0.5547946108002719 1112 1 0.17249381735181402 1112 2 -0.1761959442557881 1112 3 0.3731276885646828 1112 4 0.5332126723514321 1112 5 0.46548840440060246 1112 6 -0.010239138822655036 1112 7 0.3622950743759361 1112 8 0.36189054535567067 1112 9 -0.02162930408810837 1112 10 0.5681062024398429 1112 11 0.2027248244506552 1112 12 0.40123543326854616 1112 13 -0.053497477814588024 1112 14 0.3557522197070355 1112 15 0.6564007476847848 1112 16 0.44815346625124874 1112 17 0.45508706765136253 1112 18 0.2882987637975063 1112 19 0.33231305505246667 1112 20 0.29346244357793694 1112 21 0.27290347877616483 1112 22 0.005228173432492156 1112 23 0.4180803593615323 1112 24 0.19640842237340195 1112 25 0.09629805635807287 1113 1 -0.17811024292966593 1113 2 0.20652101141566703 1113 3 -1.1420552698996254 1113 4 -0.8330688287303261 1113 5 -1.0586315283926915 1113 6 -0.6744085116012997 1113 7 -0.2879372374671476 1113 8 -0.42793590330436615 1113 9 0.33953663494580866 1113 10 0.26143807308425693 1113 11 0.06083767530009965 1113 12 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0.9055658702898484 2202 22 0.9404356074597544 2202 23 1.287065197858567 2202 24 1.2667031466807188 2202 25 1.1703201227090148 2203 1 -0.43010669368387866 2203 2 -0.45460828337740367 2203 3 -0.561812346355314 2203 4 -0.9790932621143895 2203 5 -0.5844957756176399 2203 6 -0.8041910321534331 2203 7 -0.46266707757479963 2203 8 -0.29360517706051326 2203 9 -0.2082107104651456 2203 10 -0.2567283066262789 2203 11 -0.43357108734115 2203 12 -0.46839244517520484 2203 13 -0.8027743615334657 2203 14 -0.3619736637171087 2203 15 -0.5259638019408328 2203 16 -0.6636996067318606 2203 17 -0.5776351528087815 2203 18 -0.4789355398022579 2203 19 -0.424031376507358 2203 20 -0.47088326286353777 2203 21 -0.428896440615425 2203 22 -0.4653687079037212 2203 23 -0.17188582687794707 2203 24 -0.09371302402934845 2203 25 -0.18280278074717415 2204 1 0.379032614213191 2204 2 0.49139919430709256 2204 3 0.30486190416986503 2204 4 0.003361481209921635 2204 5 0.17226788859944264 2204 6 0.0926252640417431 2204 7 0.13566447355178468 2204 8 0.3285616829945468 2204 9 0.6717201247451925 2204 10 0.7295820703673215 2204 11 0.4391960351154154 2204 12 0.427415956223264 2204 13 0.2609899624793002 2204 14 0.718409169460756 2204 15 0.4775149139602419 2204 16 0.475433103208303 2204 17 0.5626594042559603 2204 18 0.4817808314731238 2204 19 0.4091593802858853 2204 20 0.6387019759142447 2204 21 0.5043716715346775 2204 22 0.7849008982665069 2204 23 0.9519911932723701 2204 24 0.8648373972539929 2204 25 0.8702114238198249 2205 1 0.5716221229559046 2205 2 0.5937298731573399 2205 3 0.7179478652991236 2205 4 0.37559046417995035 2205 5 0.3054524854809557 2205 6 0.3904404750126602 2205 7 0.26069418525926125 2205 8 0.3125216309012089 2205 9 0.7043894814778326 2205 10 0.7663484402727434 2205 11 0.5592484131194979 2205 12 0.795149397728558 2205 13 0.5753739871880583 2205 14 1.1365749429970577 2205 15 0.9280219700459824 2205 16 0.9231390747277644 2205 17 0.9419067551103026 2205 18 0.9474305393376525 2205 19 0.933629017411647 2205 20 1.0571823021381164 2205 21 1.090561242903263 2205 22 1.008874510000318 2205 23 1.2125084260627048 2205 24 1.06127982059067 2205 25 1.0644711931004096 2206 1 0.13564592508092588 2206 2 0.3297361638993266 2206 3 0.3810087658264152 2206 4 0.25160995650136186 2206 5 0.48373974539564557 2206 6 0.5041396038627397 2206 7 0.5379182340195285 2206 8 0.5173137621425852 2206 9 0.898804133126589 2206 10 0.7864524395376122 2206 11 0.47754452552793225 2206 12 0.5487849176933182 2206 13 0.2345143974190691 2206 14 0.6108104684332432 2206 15 0.5679843153816222 2206 16 0.6900379030700563 2206 17 0.5307028089463728 2206 18 0.6559029089650394 2206 19 0.6539645704154253 2206 20 0.6564238777136566 2206 21 0.8507105708643081 2206 22 0.7926968933413576 2206 23 0.9907709943942109 2206 24 0.9149709667641375 2206 25 0.9017683906758691 2207 1 -0.9590626238820477 2207 2 0.008659711567609918 2207 3 0.1548341541421152 2207 4 0.11983389768233366 2207 5 0.1840450003765497 2207 6 0.30016217157865555 2207 7 0.4777002155875252 2207 8 0.38846844290130633 2207 9 0.6913671443922169 2207 10 0.47826671905197315 2207 11 0.289168285087662 2207 12 0.48593521474251844 2207 13 0.42561422710356567 2207 14 -4.972255721204135 2207 15 -2.0874867510414177 2207 16 -0.8032548754796736 2207 17 -0.6514487098521542 2207 18 0.0760643257566187 2207 19 0.13695380808031388 2207 20 0.2903170275292979 2207 21 0.40655837372138137 2207 22 0.30647931984492516 2207 23 0.7018524431336175 2207 24 0.616829849246443 2207 25 0.45285100645941156 2208 1 0.5187087275735145 2208 2 0.8348090008747953 2208 3 0.7833144457803023 2208 4 0.7024617960997048 2208 5 0.8972791346633144 2208 6 0.7578974187875858 2208 7 0.7964831870020745 2208 8 0.9826777423737667 2208 9 1.2274556173227893 2208 10 1.1872069016763689 2208 11 0.8515217211253088 2208 12 1.0304748855979824 2208 13 0.8745302233879855 2208 14 1.3376624308192788 2208 15 1.1097686251613226 2208 16 1.093381821157024 2208 17 1.0303607087993711 2208 18 1.2110205975549917 2208 19 1.0164918139341097 2208 20 1.1048288315147872 2208 21 1.3353208393259521 2208 22 1.3278158780235927 2208 23 1.3883834559804171 2208 24 1.3611486883021229 2208 25 1.2963022920159588 2209 1 -0.08131980869429611 2209 2 0.01564333000497342 2209 3 0.49312200432423836 2209 4 0.6461454715710033 2209 5 0.6774713977677038 2209 6 0.5930469666660869 2209 7 0.6729211473723353 2209 8 0.5592066620976794 2209 9 0.7468584959188078 2209 10 0.18805942267727715 2209 11 0.4597615684562797 2209 12 0.4406694672741267 2209 13 0.4971095452949186 2209 14 0.8432180854590996 2209 15 0.6046695508065127 2209 16 0.8847745693779672 2209 17 0.7987639262635611 2209 18 0.8065332694414167 2209 19 0.7490178086905672 2209 20 0.702228464110334 2209 21 0.8292408307650732 2209 22 0.7105871063315661 2209 23 0.9122344253217705 2209 24 0.9273510461112551 2209 25 0.8956566589566961 2210 1 -0.09904957756763011 2210 2 -0.5294414566705434 2210 3 0.2095100718317333 2210 4 0.3874968283589624 2210 5 0.9138321082458489 2210 6 0.8373975389510093 2210 7 1.0107844829183676 2210 8 1.0974763299913874 2210 9 1.4310457306461402 2210 10 1.421503391055765 2210 11 1.0369948877087865 2210 12 0.7259992602860166 2210 13 0.7867480704291907 2210 14 1.1717518693788023 2210 15 0.9397207446592262 2210 16 1.1298412521370054 2210 17 1.2097147841880513 2210 18 1.2023170684477122 2210 19 1.2867966592930316 2210 20 1.514571312057555 2210 21 1.4049453748481042 2210 22 1.427928646773708 2210 23 1.6172411640017899 2210 24 1.4837930230589294 2210 25 1.5877854783801837 2211 1 -1.3643686258222374 2211 2 -0.3948794124320969 2211 3 0.2697533064394797 2211 4 0.3381445507139844 2211 5 0.5367027943098108 2211 6 0.1674354356632506 2211 7 0.25719400199007936 2211 8 0.4915900039502361 2211 9 0.45520903028901216 2211 10 0.6176813640467742 2211 11 0.412295290942744 2211 12 0.27690987338768025 2211 13 0.3024887549966948 2211 14 0.7440698782038382 2211 15 0.443759150975076 2211 16 0.3709589827022981 2211 17 0.6137317547968664 2211 18 0.4998082818300702 2211 19 0.3871756264155053 2211 20 0.4067699280388837 2211 21 0.5294706548267527 2211 22 0.43912860147560906 2211 23 0.7221523675965216 2211 24 0.4315101202597518 2211 25 0.6573326187355306 2212 1 1.1381235233041025 2212 2 0.5441575470654456 2212 3 0.686089565397527 2212 4 0.5489248067732433 2212 5 0.812096948428497 2212 6 0.8007682321847156 2212 7 0.9961850640723711 2212 8 0.8308926853255514 2212 9 0.8913238543489257 2212 10 0.78389442467968 2212 11 0.6670903630097413 2212 12 0.9538572926645941 2212 13 0.6282549197442577 2212 14 0.778696659748249 2212 15 0.8271885736339044 2212 16 -0.009834962059763086 2212 17 0.514438736035288 2212 18 0.608315408007698 2212 19 0.6305035916300952 2212 20 0.7652520924643653 2212 21 0.8484198455828533 2212 22 0.7351806185462237 2212 23 0.9099476812288572 2212 24 0.5574791998957949 2212 25 0.24090295451135546 2213 1 0.3124697897064408 2213 2 0.12422929255775372 2213 3 -0.2603429843995073 2213 4 -0.8827715965119438 2213 5 -1.874548949806183 2213 6 -0.8615483920010689 2213 7 -0.5772039664194608 2213 8 -0.3557022358488098 2213 9 -0.8510692354273459 2213 10 -0.682583438059865 2213 11 -0.7052404214705774 2213 12 -0.8106541771739728 2213 13 -0.9803951321768167 2213 14 -8.231443055157822 2213 15 -3.960036008637406 2213 16 -2.377742070527617 2213 17 -1.468980503084886 2213 18 -5.628619360235483 2213 19 -3.296224436312883 2213 20 -5.450506427312849 2213 21 -2.4908995162598764 2213 22 -2.0941868882979193 2213 23 -4.842984443759345 2213 24 -2.785414220287347 2213 25 -2.2524341549005165 2214 1 0.5214983676378502 2214 2 0.2794626529184967 2214 3 -1.5722250879856183 2214 4 -1.9383022583990268 2214 5 -0.41333684152584027 2214 6 -0.3427394473503611 2214 7 -0.41958081388528257 2214 8 -0.3774095147574741 2214 9 -5.3892739040570525 2214 10 -1.4241587094008494 2214 11 -0.9511329661724943 2214 12 -1.1676080456500557 2214 13 -1.3246674546849633 2214 14 -2.718430779023027 2214 15 -2.0591377028293607 2214 16 -1.4425331770867444 2214 17 -1.6069518824786186 2214 18 -0.978048228355938 2214 19 -4.50566909413163 2214 20 -2.492278003010938 2214 21 -3.935237273553721 2214 22 -6.2453590244591695 2214 23 -6.297827329148895 2214 24 -5.769631634475314 2214 25 -3.534817261345843 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21231.0 3 15.0 4 375.0 5 2346.0 6 3000.0 7 2565.0 8 2012.0 9 1550.0 10 1394.0 11 1227.0 12 1239.0 13 1162.0 14 1190.0 15 1213.0 16 1303.0 17 1318.0 18 1573.0 19 1845.0 20 2322.0 21 3077.0 22 3918.0 23 4771.0 24 5801.0 25 6781.0 26 7780.0 27 8607.0 28 9972.0 29 12386.0 30 15232.0 31 19099.0 32 24815.0 33 32212.0 34 41918.0 35 55713.0 36 75370.0 37 111238.0 38 175279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37335737161339 18.88545125378052 9.352129094019572 29.38906228058652 2 12.993854920590845 21.253342669668783 38.90565673272526 26.84714567701511 3 19.637884900099152 24.911599183226258 28.038037630148793 27.412478286525797 4 12.50056590960537 17.57413415830378 35.39319399381272 34.53210593827812 5 13.06987258032389 38.77049971932662 34.769696933115235 13.389930767234251 6 33.55757144371583 35.8633066287047 17.081522315276157 13.497599612303313 7 28.33538192192383 31.614181978165522 20.73126297376446 19.31917312614619 8 26.167495192133238 37.76791005962964 19.345767262963097 16.71882748527402 9 26.900833256473955 15.664495956188643 19.03108798105782 38.40358280627959 10 15.586857293749294 30.02783960164472 32.63706665660719 21.748236447998792 11 34.04547744985924 23.53711487340722 22.21310312989376 20.204304546839783 12 24.156862626768575 25.73888615646433 30.34811759416555 19.756133622601542 13 30.026144717726062 21.50474693331362 24.54088336861035 23.928224980349974 14 21.44388579366158 23.110929927073425 26.11012969757364 29.335054581691356 15 25.458972691709448 29.390998417413577 22.308283006884025 22.841745883992946 16 21.860677032258454 27.972011091766568 28.671582930266098 21.49572894570888 17 22.668316196827167 28.956496156544436 27.3412641536477 21.033923492980698 18 22.87385074032389 27.061123868090487 29.27334502080511 20.791680370780515 19 23.947214775276578 27.400552473685085 27.148001695741186 21.504231055297154 20 23.837843464722308 27.302475254246495 27.528170405874107 21.33151087515709 21 22.313528420499992 27.078414540868284 27.39960383134017 23.208453207291555 22 22.95470009006576 28.18047549291015 25.364298656255045 23.500525760769044 23 21.625136343269734 27.920989546638825 27.117035704964483 23.336838405126958 24 22.952924350680696 28.4537329825239 27.062765520903735 21.530577145891666 25 22.963786569469924 26.779442647484792 26.402131408709085 23.854639374336198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 500.0 1 500.0 2 511.0 3 522.0 4 522.0 5 522.0 6 599.5 7 677.0 8 677.0 9 677.0 10 995.0 11 1313.0 12 1313.0 13 1313.0 14 1817.5 15 2322.0 16 2322.0 17 2322.0 18 4066.5 19 5811.0 20 5811.0 21 5811.0 22 9426.5 23 13042.0 24 13042.0 25 13042.0 26 19164.0 27 25286.0 28 25286.0 29 25286.0 30 34076.5 31 42867.0 32 42867.0 33 42867.0 34 49183.5 35 55500.0 36 55500.0 37 55500.0 38 60154.0 39 64808.0 40 64808.0 41 64808.0 42 70423.5 43 76039.0 44 76039.0 45 76039.0 46 91988.0 47 107937.0 48 107937.0 49 107937.0 50 101495.5 51 95054.0 52 95054.0 53 95054.0 54 80109.0 55 65164.0 56 65164.0 57 65164.0 58 55329.5 59 45495.0 60 45495.0 61 45495.0 62 37745.5 63 29996.0 64 29996.0 65 29996.0 66 23592.5 67 17189.0 68 17189.0 69 17189.0 70 12715.5 71 8242.0 72 8242.0 73 8242.0 74 5755.5 75 3269.0 76 3269.0 77 3269.0 78 2259.0 79 1249.0 80 1249.0 81 1249.0 82 823.5 83 398.0 84 398.0 85 398.0 86 265.0 87 132.0 88 132.0 89 132.0 90 83.0 91 34.0 92 34.0 93 34.0 94 18.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08629416352743988 2 0.03092710406140765 3 0.036509069184686105 4 0.030021920527903038 5 0.023685635793370737 6 0.38304349859470255 7 0.21392504175159047 8 0.216188000585352 9 0.22146823786412892 10 0.01870712635909536 11 0.0022629588337615353 12 0.0040733259007707644 13 0.0 14 0.0813156540931645 15 0.0015086392225076902 16 0.0022629588337615353 17 0.005129373356526147 18 0.00467678158977384 19 0.0018103670670092283 20 0.0012069113780061522 21 0.0 22 0.0 23 0.0 24 1.5086392225076902E-4 25 1.5086392225076902E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 662849.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.52069578593225 #Duplication Level Percentage of deduplicated Percentage of total 1 89.68319252355576 65.03887522111981 2 7.193208657060272 10.433129934868044 3 1.4643187862072702 3.18580251684489 4 0.5591754445069917 1.6220716920821994 5 0.2920239172440518 1.0588888832336065 6 0.17564466805651963 0.7642724123128746 7 0.1252791146665955 0.6359729994146965 8 0.08805378680792537 0.5108577508717512 9 0.057698570532944464 0.37659064328125375 >10 0.3125484031273711 4.083183023919039 >50 0.023065195616005278 1.1480818529522048 >100 0.017613319837239676 2.6755505998740867 >500 0.004822694717053815 2.393625574957892 >1k 0.002935553306032757 3.729804212093715 >5k 4.193647580046796E-4 2.3432926821740114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7918 1.194540536381589 No Hit GTACATGGGAAGCAGTGGTATCAAC 7492 1.1302725055027616 No Hit GAGTACATGGGAAGCAGTGGTATCA 3405 0.5136916552638686 No Hit CATGTACTCTGCGTTGATACCACTG 2855 0.4307164980259456 No Hit GCGTTGATACCACTGCTTCCCATGT 2275 0.3432154231204995 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2090 0.31530559750410725 No Hit GTATCAACGCAGAGTACATGGGAAG 1989 0.3000683413567796 No Hit GTATCAACGCAGAGTACTTTTTTTT 1732 0.2612963133383319 No Hit ACTCTGCGTTGATACCACTGCTTCC 1658 0.25013238309177505 No Hit GCTTCCCATGTACTCTGCGTTGATA 1434 0.21633886450760278 No Hit TATCAACGCAGAGTACATGGGAAGC 1386 0.20909739623956586 No Hit GTCCTACAGTGGACATTTCTAAATT 1296 0.19551964323699664 No Hit GGTATCAACGCAGAGTACATGGGAA 1226 0.18495916867944281 No Hit CTGTAGGACGTGGAATATGGCAAGA 1093 0.16489426702009055 No Hit CAGTGGTATCAACGCAGAGTACATG 1050 0.15840711836330748 No Hit GGTATCAACGCAGAGTACTTTTTTT 1039 0.15674761521854902 No Hit CTTTAGGACGTGAAATATGGCGAGG 871 0.13140247628041982 No Hit TATCAACGCAGAGTACTTTTTTTTT 857 0.12929038136890905 No Hit CATGGGAAGCAGTGGTATCAACGCA 856 0.1291395174466583 No Hit GTCCTAAAGTGTGTATTTCTCATTT 842 0.1270274225351475 No Hit GCAGTGGTATCAACGCAGAGTACAT 801 0.120842001722866 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 783 0.11812645112235215 No Hit GTCCTACAGTGTGCATTTCTCATTT 783 0.11812645112235215 No Hit GTGGTATCAACGCAGAGTACATGGG 775 0.11691953974434599 No Hit ATACCACTGCTTCCCATGTACTCTG 745 0.11239362207682294 No Hit ACATGGGAAGCAGTGGTATCAACGC 715 0.10786770440929985 No Hit GATATACACTGTTCTACAAATCCCG 689 0.10394524243077988 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.5086392225076902E-4 0.0 11 0.0 0.0 0.0 1.5086392225076902E-4 0.0 12 0.0 0.0 0.0 3.0172784450153804E-4 0.0 13 0.0 0.0 0.0 3.0172784450153804E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCCGT 25 0.0060255793 19.001589 8 ATTATGC 35 0.0021076596 16.35514 3 CCGGTTT 55 1.1490874E-5 15.512714 12 CGGTTTC 50 8.859792E-5 15.167987 13 ATTGTAT 45 6.5405975E-4 14.8407755 3 GCTCTAG 45 6.56703E-4 14.832887 1 AATCCCG 150 0.0 14.523926 19 GTAGCAC 40 0.00512803 14.310748 3 TGCCGGT 60 2.6134858E-5 14.221063 10 GCCGGTT 60 2.6153473E-5 14.219988 11 TAGGACC 205 0.0 13.494265 4 GCTCTAC 65 5.2563613E-5 13.202898 1 CAAATCC 185 0.0 12.801136 17 GATATAC 360 0.0 12.713902 1 GTATAAG 75 1.4201174E-5 12.713902 1 GTAGGAC 595 0.0 12.667215 3 TCCAACG 75 1.5208074E-5 12.629501 18 CCAACGA 60 4.1890738E-4 12.629501 19 CCCTACA 70 1.04517E-4 12.268219 2 TGTAGGA 630 0.0 12.268219 2 >>END_MODULE