FastQCFastQC Report
Thu 26 May 2016
SRR1926561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635599
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12610.19839552925665394No Hit
CTGTAGGACGTGGAATATGGCAAGA9230.1452173461569323No Hit
GTCCTAAAGTGTGTATTTCTCATTT8120.12775350496146157No Hit
GTACATGGGAAGCAGTGGTATCAAC7340.11548161655383347No Hit
GTCCTACAGTGTGCATTTCTCATTT7230.11375096562455259No Hit
CCCATGTACTCTGCGTTGATACCAC6750.10619903429678146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTGT554.3414366E-721.8503951
GTCTTAC602.2417227E-517.8040241
TGTAGGA7150.016.8507842
ACGTGCA506.8488385E-515.6309528
CAGGAGT556.914037E-515.6130364
CTGTAGG7400.015.3379281
TAGGACC2450.014.8961114
GTAGGAC7800.014.6152453
GCCTCGG450.004455906314.6152453
GACCTAC751.5105135E-414.243221
GATATAC3750.013.8871381
TGTAGAA3750.013.8738132
CAGTCTA400.00655383713.7900389
TCCTATA700.001201776713.6912622
TATTGGA802.1123535E-413.6912622
GTCCTAC8600.013.6635541
GATCTAC600.008103630513.3530181
ATAGGAG500.0089541713.153723
GTAAGAC500.0089541713.153723
TTGTAGA1759.458745E-1112.9715031
Produced by FastQC (version 0.11.5)