##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926561_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635599 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.368587741642138 27.0 2.0 30.0 2.0 31.0 2 15.812378559437633 16.0 2.0 30.0 2.0 31.0 3 19.17383759257016 19.0 10.0 30.0 2.0 31.0 4 21.222663975242252 27.0 2.0 35.0 2.0 37.0 5 21.458816014499707 28.0 2.0 35.0 2.0 35.0 6 22.4399078664378 30.0 2.0 35.0 2.0 37.0 7 22.10532112227993 29.0 2.0 35.0 2.0 37.0 8 22.33138661325773 30.0 2.0 35.0 2.0 37.0 9 25.090981892671323 31.0 17.0 37.0 2.0 39.0 10 25.747411496871454 31.0 17.0 37.0 2.0 39.0 11 22.96266513949833 29.0 2.0 37.0 2.0 39.0 12 24.076670982805197 30.0 2.0 37.0 2.0 39.0 13 20.830721885968984 27.0 2.0 35.0 2.0 39.0 14 24.053357541468756 30.0 8.0 37.0 2.0 40.0 15 25.139290653383657 31.0 8.0 38.0 2.0 40.0 16 25.48480567150043 31.0 2.0 38.0 2.0 40.0 17 24.804933613803673 30.0 2.0 38.0 2.0 40.0 18 24.88365463130055 30.0 2.0 38.0 2.0 40.0 19 25.27199539332189 31.0 2.0 38.0 2.0 40.0 20 25.536791278777972 31.0 2.0 38.0 2.0 40.0 21 23.414322552426924 30.0 2.0 37.0 2.0 40.0 22 24.31087997306478 30.0 2.0 37.0 2.0 40.0 23 24.274062734522868 30.0 2.0 38.0 2.0 40.0 24 23.38561734678626 30.0 2.0 38.0 2.0 40.0 25 23.170659488136387 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.338753168856279 1101 2 9.990854100919387 1101 3 6.772465938792095 1101 4 8.094201527575358 1101 5 7.640706233218914 1101 6 7.233103741297928 1101 7 6.752194165125207 1101 8 5.3485754263948735 1101 9 5.333926536580396 1101 10 4.937455952856514 1101 11 7.599269860834564 1101 12 5.409602185674554 1101 13 9.570273705306423 1101 14 6.8195406744492 1101 15 6.155623211854984 1101 16 5.7597018488093426 1101 17 1.8822195358776597 1101 18 4.246454066472342 1101 19 4.833858959035403 1101 20 3.2129131996181286 1101 21 0.7112087868248871 1101 22 4.187118490788002 1101 23 5.189489707076749 1101 24 6.571730529479332 1101 25 6.911667561351688 1102 1 4.301075388837642 1102 2 8.941540719728026 1102 3 5.159960125991766 1102 4 6.4417836526518215 1102 5 6.218114399307495 1102 6 5.129790903467974 1102 7 5.155224988384408 1102 8 5.192058173003449 1102 9 4.203162280427161 1102 10 3.564195001040673 1102 11 6.37621583637663 1102 12 3.8457847217722545 1102 13 8.245451922847664 1102 14 5.924431093787533 1102 15 3.894933909574366 1102 16 4.363019686897644 1102 17 5.218801925967814 1102 18 5.367830337579683 1102 19 4.058522233304572 1102 20 4.451253847218258 1102 21 3.850392644657539 1102 22 4.438003119827169 1102 23 4.6538470322052135 1102 24 5.904242709275291 1102 25 5.556019186185637 1103 1 4.597276277656615 1103 2 9.027822676087323 1103 3 5.889727866890265 1103 4 7.645417756078341 1103 5 6.500340926740677 1103 6 6.412842469673702 1103 7 6.383510224379698 1103 8 5.987666572838613 1103 9 5.352885871064299 1103 10 4.614082060499889 1103 11 5.595307661054612 1103 12 4.534472371497554 1103 13 8.331064443601875 1103 14 6.614098530490388 1103 15 5.831648733426448 1103 16 5.333797070062747 1103 17 3.419174347614778 1103 18 2.2002765048684765 1103 19 4.078844690945459 1103 20 4.708982280362758 1103 21 6.802198410866346 1103 22 2.462405458320486 1103 23 4.756117641502758 1103 24 6.112272174701463 1103 25 2.2676574481495564 1104 1 5.601934814719389 1104 2 10.32299980517237 1104 3 6.875328442867225 1104 4 7.298598437361726 1104 5 7.373188566026592 1104 6 7.23752437601447 1104 7 6.873825079870585 1104 8 6.8992675556078105 1104 9 5.9471769930364395 1104 10 5.217700468955041 1104 11 8.012654371441513 1104 12 5.0892467762660445 1104 13 9.271454062059693 1104 14 7.135422358388336 1104 15 6.278610429426696 1104 16 5.692318899402341 1104 17 5.9979988542089835 1104 18 3.8090708764978665 1104 19 4.612484621943963 1104 20 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2204 8 4.777987283305439 2204 9 3.10493426920468 2204 10 2.531068721050211 2204 11 4.225967998091324 2204 12 4.203346622153269 2204 13 2.216157792666184 2204 14 3.5284242412407423 2204 15 3.6245990784443194 2204 16 3.742238476269769 2204 17 -1.5722579695528331 2204 18 1.9335966914396536 2204 19 2.713916246289237 2204 20 2.9337343609225037 2204 21 4.326218476323678 2204 22 3.9163487675636475 2204 23 2.835823413580222 2204 24 4.412313730883135 2204 25 4.511105578671515 2205 1 5.376293672074571 2205 2 9.746033760696347 2205 3 6.608755863963474 2205 4 7.165399336627793 2205 5 7.217154984517773 2205 6 6.8481248944206605 2205 7 6.8164402362032135 2205 8 6.407757047737604 2205 9 5.228842998318282 2205 10 4.975521989575174 2205 11 7.225287283308386 2205 12 6.162743606100555 2205 13 9.182816544672875 2205 14 6.60473804103118 2205 15 5.727821371529313 2205 16 5.273306217925544 2205 17 6.122857172542325 2205 18 5.726473011236216 2205 19 4.476127985863968 2205 20 4.899076253974108 2205 21 -0.6574865083723935 2205 22 3.4213427019087064 2205 23 4.588246636651267 2205 24 6.308712992793822 2205 25 6.672755080250528 2206 1 4.444174452614117 2206 2 8.528515868989873 2206 3 6.158117377139529 2206 4 2.812878867022505 2206 5 6.01622507459259 2206 6 6.258159336393049 2206 7 6.178709284853568 2206 8 6.284241866907529 2206 9 5.200951144089711 2206 10 4.19115642097157 2206 11 3.5713159582528426 2206 12 4.663285237027875 2206 13 7.866885907648026 2206 14 0.2078038353856222 2206 15 2.2825043210810385 2206 16 3.252931874356566 2206 17 4.390577602332105 2206 18 -1.0262306719318524 2206 19 0.8230447333410886 2206 20 2.163762306336171 2206 21 3.1836534905855594 2206 22 -1.130564826566772 2206 23 -1.9138510653249696 2206 24 1.5362430857770377 2206 25 2.8299306985379786 2207 1 1.1016139454659495 2207 2 -13.329254104671234 2207 3 -6.023198208325953 2207 4 -4.414854968696449 2207 5 0.7360987300880772 2207 6 1.511983928685467 2207 7 2.564303419332866 2207 8 2.6587298863855082 2207 9 0.3393595415580819 2207 10 0.1799380910613202 2207 11 -1.5700574415981166 2207 12 -2.3627383486493905 2207 13 0.4723943952834446 2207 14 -5.3567430495048 2207 15 -1.420163311792873 2207 16 -0.48297507893753533 2207 17 0.45239774395996335 2207 18 0.3981139574388983 2207 19 -0.08287175822027137 2207 20 -0.12437950169888978 2207 21 -3.797815244523246 2207 22 -4.383769075876689 2207 23 -1.386730223302223 2207 24 0.30251839636340705 2207 25 1.0734378411749823 2208 1 5.231141525883565 2208 2 6.832979937881957 2208 3 6.765961292730534 2208 4 8.64133203298244 2208 5 4.26421696744373 2208 6 6.789345707416533 2208 7 7.15370757251414 2208 8 7.068455965376387 2208 9 5.878556546364944 2208 10 5.149581597965202 2208 11 7.400542011964262 2208 12 6.8496126736868845 2208 13 9.355231881159693 2208 14 4.0224781172927315 2208 15 5.33546464461979 2208 16 5.977491180454361 2208 17 6.622419799356994 2208 18 1.471360377825615 2208 19 4.06866892776322 2208 20 5.143803433615918 2208 21 7.684174975775381 2208 22 6.702692229828351 2208 23 2.665807743260391 2208 24 4.037688455922556 2208 25 5.353958712411043 2209 1 2.9891645755289566 2209 2 4.985031609614479 2209 3 5.283710786144923 2209 4 0.6409964200138489 2209 5 4.975881101182331 2209 6 5.593904977933249 2209 7 6.4485277346215355 2209 8 4.767949379763419 2209 9 4.386782230255523 2209 10 3.5496765827676313 2209 11 -0.44240378337515196 2209 12 3.128006797293775 2209 13 2.056352701828388 2209 14 3.618932018001953 2209 15 4.002637896899426 2209 16 3.7990970568474722 2209 17 1.4054998398838414 2209 18 3.3372734983929924 2209 19 4.166731237793616 2209 20 4.274496189441653 2209 21 6.232287944367073 2209 22 4.999253779980325 2209 23 0.05735409584397644 2209 24 -3.1023951307035844 2209 25 -0.11958485395167173 2210 1 -2.3081302005904476 2210 2 -13.329254104671234 2210 3 -7.460572262895589 2210 4 -2.248249315593828 2210 5 -0.8696945917814212 2210 6 -1.3806685828343426 2210 7 -1.7983539284914691 2210 8 -19.86062204880801 2210 9 -12.80568845204725 2210 10 -7.547484931091642 2210 11 -10.01148693902547 2210 12 -5.070527317844817 2210 13 -5.440675549770761 2210 14 -4.435359026774169 2210 15 -6.046402614827656 2210 16 -5.592107633984085 2210 17 -5.053561993804859 2210 18 -5.304051128472679 2210 19 -9.922181342163741 2210 20 -7.480607755121248 2210 21 -5.720483910643587 2210 22 -10.024880015328844 2210 23 -8.27288311097905 2210 24 -10.843146729850707 2210 25 -8.724941996808816 2211 1 -0.6545967984473862 2211 2 3.8418104995143896 2211 3 0.2564644258173523 2211 4 0.9957564291130296 2211 5 0.31231194740616885 2211 6 -0.6151032113297177 2211 7 -19.62111512252132 2211 8 -13.244607490118293 2211 9 -9.688985513147571 2211 10 -7.746791797578137 2211 11 -9.665868296568963 2211 12 -5.568239790513324 2211 13 -7.233439154404554 2211 14 -5.570581175810606 2211 15 -6.099614150416318 2211 16 -12.04642159647373 2211 17 -8.655851388505603 2211 18 -6.787641196784442 2211 19 -9.225796059749607 2211 20 -7.232345257328138 2211 21 -5.05255054516887 2211 22 -6.6697691954518845 2211 23 -8.861555139824812 2211 24 -20.97560941641787 2211 25 -20.724941996808816 2212 1 -1.3478418469258493 2212 2 -13.329254104671234 2212 3 -7.641898327624277 2212 4 -18.686682151414722 2212 5 -18.868761755960527 2212 6 -19.848952164923894 2212 7 -9.528822068287258 2212 8 -9.24596933710487 2212 9 -5.397801329619426 2212 10 -5.391309043166853 2212 11 -5.105243595798694 2212 12 -3.0565827092985884 2212 13 -5.929656371163322 2212 14 -3.795411464395766 2212 15 -3.876473833425436 2212 16 -3.8541821330397106 2212 17 -4.281978072308629 2212 18 -3.4208437708743737 2212 19 -3.8891220312019 2212 20 -3.9895287188089945 2212 21 -2.039271454844563 2212 22 -3.2120951054349263 2212 23 -3.4649061664912146 2212 24 -2.457055658092468 2212 25 -2.8885956599867413 2213 1 -17.892085424471045 2213 2 -13.329254104671234 2213 3 -8.00846937595173 2213 4 -5.080107390014934 2213 5 -18.868761755960527 2213 6 -10.048315897691657 2213 7 -19.62111512252132 2213 8 -9.821385569486695 2213 9 -7.562143121963839 2213 10 -6.608679158786678 2213 11 -5.8533509883440935 2213 12 -4.432563570832269 2213 13 -18.301683012457328 2213 14 -11.550924821870796 2213 15 -6.296123654380686 2213 16 -5.744044133881204 2213 17 -4.198207931665923 2213 18 -4.245606936389617 2213 19 -5.132748007775238 2213 20 -4.98351112005691 2213 21 -3.3019057384082657 2213 22 -4.048029775541565 2213 23 -3.707680557996351 2213 24 -2.8981969031623045 2213 25 -3.715397988325254 2214 1 -17.892085424471045 2214 2 -13.329254104671234 2214 3 -11.838793493344651 2214 4 -18.686682151414722 2214 5 -18.868761755960527 2214 6 -19.848952164923894 2214 7 -15.765995296813259 2214 8 -14.264761482359209 2214 9 -13.441067520755993 2214 10 -13.394217363699843 2214 11 -13.981654483655262 2214 12 -11.867394696637131 2214 13 -18.301683012457328 2214 14 -15.991688897030729 2214 15 -22.693790674529147 2214 16 -17.41770188495147 2214 17 -22.383785874401873 2214 18 -16.91497323363315 2214 19 -14.243387809825434 2214 20 -13.16516495279302 2214 21 -10.72593256605932 2214 22 -11.640271196677004 2214 23 -11.617457217570278 2214 24 -10.573648632104145 2214 25 -10.272872280033218 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 132501.0 3 11.0 4 656.0 5 2181.0 6 3053.0 7 2845.0 8 2566.0 9 2386.0 10 2239.0 11 1994.0 12 1983.0 13 2058.0 14 3172.0 15 6452.0 16 9434.0 17 10278.0 18 10670.0 19 12454.0 20 14826.0 21 16845.0 22 17668.0 23 18856.0 24 19472.0 25 19640.0 26 16284.0 27 13607.0 28 14228.0 29 16370.0 30 16337.0 31 18862.0 32 23690.0 33 29318.0 34 35176.0 35 38585.0 36 37406.0 37 37181.0 38 24315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.724960358209394 18.805448614442312 12.832328832735229 25.63726219461307 2 12.345498409423946 21.262394219873894 43.441198275284954 22.950909095417206 3 20.706508960597382 24.616587819190535 28.69208796009878 25.984815260113294 4 10.98833260188259 17.029265920890417 38.65319583273391 33.329205644493086 5 11.434130082397797 38.29737964433472 36.25265983148475 14.01583044178274 6 30.082012957973443 38.11569777541238 18.917865075933133 12.88442419068105 7 26.19927223280225 30.864544417040435 22.13047082860701 20.805712521550305 8 23.83016648369566 39.92586686426397 20.16141286624791 16.08255378579246 9 25.73007675236534 16.141937536521556 20.40986046599538 37.718125245117726 10 14.50806065442937 30.516041500399044 34.73790901835594 20.23798882681564 11 32.82300672430355 22.064121037463977 25.542847422350306 19.570024815882164 12 24.43858124707095 24.693252301128926 32.70246515576296 18.16570129603717 13 28.94351984830991 21.85651394403612 25.003895506556656 24.196070701097312 14 19.206341847851284 24.3526190175507 28.07744753092834 28.36359160366968 15 21.980062200227657 34.06808298120048 23.207851886924008 20.744002931647852 16 19.63764155640282 28.69968392561468 31.439914243132392 20.222760274850113 17 20.05294667883608 29.46468086899993 30.41013411753644 20.07223833462755 18 19.613686745756432 28.353169659684024 33.37022698379235 18.662916610767198 19 22.499968073150796 27.073808489987737 27.363863914997953 23.062359521863506 20 22.299235407903414 29.07343302798035 29.066490796810363 19.56084076730588 21 20.88727959490539 26.800667261103744 26.930631825196016 25.38142131879485 22 21.287274355243593 30.204819084469886 28.166541419503382 20.34136514078314 23 20.859754733801434 29.61449168533096 29.063698019536872 20.462055561330725 24 22.733447225523864 27.95856911673488 29.541335588075277 19.766648069665983 25 21.52259414028229 28.42960132435102 29.560371536533847 20.487432998832844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 844.0 1 844.0 2 1650.5 3 2457.0 4 2457.0 5 2457.0 6 3365.0 7 4273.0 8 4273.0 9 4273.0 10 4979.0 11 5685.0 12 5685.0 13 5685.0 14 7031.5 15 8378.0 16 8378.0 17 8378.0 18 11861.5 19 15345.0 20 15345.0 21 15345.0 22 21648.0 23 27951.0 24 27951.0 25 27951.0 26 36613.5 27 45276.0 28 45276.0 29 45276.0 30 55210.5 31 65145.0 32 65145.0 33 65145.0 34 70320.5 35 75496.0 36 75496.0 37 75496.0 38 77058.0 39 78620.0 40 78620.0 41 78620.0 42 78492.5 43 78365.0 44 78365.0 45 78365.0 46 77459.5 47 76554.0 48 76554.0 49 76554.0 50 68049.5 51 59545.0 52 59545.0 53 59545.0 54 49032.5 55 38520.0 56 38520.0 57 38520.0 58 31669.5 59 24819.0 60 24819.0 61 24819.0 62 19665.5 63 14512.0 64 14512.0 65 14512.0 66 11148.0 67 7784.0 68 7784.0 69 7784.0 70 5620.5 71 3457.0 72 3457.0 73 3457.0 74 2503.5 75 1550.0 76 1550.0 77 1550.0 78 1105.0 79 660.0 80 660.0 81 660.0 82 446.0 83 232.0 84 232.0 85 232.0 86 158.0 87 84.0 88 84.0 89 84.0 90 60.0 91 36.0 92 36.0 93 36.0 94 21.5 95 7.0 96 7.0 97 7.0 98 5.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.280857899398836 2 36.54599834172175 3 5.651047279810069 4 13.603230967953065 5 13.713363299816395 6 14.862358184956237 7 9.289032865061147 8 7.850232615218086 9 0.11013233186332892 10 1.431562982320614 11 5.661273853483093 12 5.664263159633669 13 16.194802068599856 14 1.5796123027254607 15 2.970583654159305 16 2.9849008573015374 17 2.95028783871592 18 1.490719777721488 19 1.442261551701623 20 0.2827254290834315 21 4.3645443117437255 22 1.5999081181688455 23 0.10966033615534322 24 9.195263051074654 25 0.11359363372189069 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 635599.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.96320426912145 #Duplication Level Percentage of deduplicated Percentage of total 1 96.8414079322632 86.15321955587248 2 2.082776076131591 3.7058086701546795 3 0.43822663398545003 1.1695813666625132 4 0.1828463217250677 0.6506637867793879 5 0.103169291826845 0.45891353915461064 6 0.060972971617395774 0.3254610557336235 7 0.04728137902367859 0.29444120861464906 8 0.03566897457663427 0.2538581017064971 9 0.024657790990775096 0.1974272487063865 >10 0.1538249240871783 2.6030073359958448 >50 0.01523414258721033 0.9186129191364485 >100 0.012447314658670692 2.232060032474682 >500 0.00130046571038342 0.8285314284934764 >1k 1.8578081576906002E-4 0.20841375051471647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1261 0.19839552925665394 No Hit CTGTAGGACGTGGAATATGGCAAGA 923 0.1452173461569323 No Hit GTCCTAAAGTGTGTATTTCTCATTT 812 0.12775350496146157 No Hit GTACATGGGAAGCAGTGGTATCAAC 734 0.11548161655383347 No Hit GTCCTACAGTGTGCATTTCTCATTT 723 0.11375096562455259 No Hit CCCATGTACTCTGCGTTGATACCAC 675 0.10619903429678146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5733190266189846E-4 0.0 5 0.0 0.0 0.0 1.5733190266189846E-4 0.0 6 0.0 0.0 0.0 1.5733190266189846E-4 0.0 7 0.0 0.0 0.0 1.5733190266189846E-4 0.0 8 0.0 0.0 0.0 1.5733190266189846E-4 0.0 9 0.0 0.0 0.0 1.5733190266189846E-4 0.0 10 0.0 0.0 0.0 1.5733190266189846E-4 0.0 11 0.0 0.0 0.0 3.146638053237969E-4 0.0 12 0.0 0.0 0.0 3.146638053237969E-4 0.0 13 0.0 0.0 0.0 3.146638053237969E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTGT 55 4.3414366E-7 21.850395 1 GTCTTAC 60 2.2417227E-5 17.804024 1 TGTAGGA 715 0.0 16.850784 2 ACGTGCA 50 6.8488385E-5 15.630952 8 CAGGAGT 55 6.914037E-5 15.613036 4 CTGTAGG 740 0.0 15.337928 1 TAGGACC 245 0.0 14.896111 4 GTAGGAC 780 0.0 14.615245 3 GCCTCGG 45 0.0044559063 14.615245 3 GACCTAC 75 1.5105135E-4 14.24322 1 GATATAC 375 0.0 13.887138 1 TGTAGAA 375 0.0 13.873813 2 CAGTCTA 40 0.006553837 13.790038 9 TCCTATA 70 0.0012017767 13.691262 2 TATTGGA 80 2.1123535E-4 13.691262 2 GTCCTAC 860 0.0 13.663554 1 GATCTAC 60 0.0081036305 13.353018 1 ATAGGAG 50 0.00895417 13.15372 3 GTAAGAC 50 0.00895417 13.15372 3 TTGTAGA 175 9.458745E-11 12.971503 1 >>END_MODULE