FastQCFastQC Report
Thu 26 May 2016
SRR1926561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635599
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA27560.4336067237361922No Hit
GTCCTACAGTGGACATTTCTAAATT25490.4010390198851792No Hit
GTACATGGGAAGCAGTGGTATCAAC19400.305223891164083No Hit
CTTTAGGACGTGAAATATGGCGAGG19300.30365057213746405No Hit
CCCATGTACTCTGCGTTGATACCAC19000.2989306150576071No Hit
GTCCTAAAGTGTGTATTTCTCATTT17590.27674681678227936No Hit
GTCCTACAGTGTGCATTTCTCATTT16160.2542483547016279No Hit
GTATCAACGCAGAGTACTTTTTTTT14830.2333232116475954No Hit
GAGTAAGCAGTGGTATCAACGCAGA14510.22828859076241464No Hit
CTGTAGGACCTGGAATATGGCGAGA14130.22230997846126255No Hit
GATATACACTGTTCTACAAATCCCG12850.2021714949205395No Hit
CTGAAGGACCTGGAATATGGCGAGA11080.1743237481493835No Hit
GTGTATATCAATGAGTTACAATGAA10140.15953454929916502No Hit
GAGTACATGGGAAGCAGTGGTATCA9190.14458801854628467No Hit
GTGTATATCAATGAGTTACAATGAG8870.13955339766110395No Hit
GGTATCAACGCAGAGTACTTTTTTT8740.13750808292649924No Hit
GTTCTACAGTGTGGTTTTTATCATT8470.133260121554628No Hit
TATCAACGCAGAGTACTTTTTTTTT7840.12334821168692839No Hit
CTGTAGAACATATTAGATGAGTGAG7720.1214602288549856No Hit
GATATACACTGTTCTACAATGCCGG7660.12051623743901423No Hit
CATGTACTCTGCGTTGATACCACTG7580.11925758221771902No Hit
GAATATGGCAAGAAAACTGAAAATC7070.11123365518196221No Hit
GTCCTTCAGTGTGCATTTCTCATTT6900.10855901283670993No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6650.10462571527016248No Hit
TTGTAGAACAGTGTATATCAATGAG6620.10415371956217678No Hit
ACCTGGAATATGGCGAGAAAACTGA6560.10320972814620538No Hit
GCTTACTCTGCGTTGATACCACTGC6400.10069241770361502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCG351.0120172E-418.9525616
TAGGACC4800.015.290554
TCGTCAA456.565888E-414.833072
TAGAACG400.00515896114.2976584
TAGAACC400.00515896114.2976584
GTGCTAG400.00516167114.2965271
GCCTAAG400.00516167114.2965271
ATTCCCG551.9859243E-413.79453311
CTAGACT707.0005917E-613.61681654
ACTGTTC5100.013.414168
TAGGTTC500.001460613213.34448055
GATATAC6100.013.2809251
AATCCCG2950.013.17042419
TGTAGGA12400.013.1498232
CTAGACA801.92068E-613.1061864
GGACCTG8050.012.9063746
AGGACCT8450.012.8594325
CTGTAGG12100.012.7605361
GTATTAT603.975223E-412.7080241
GTCTAGG603.975223E-412.7080241
Produced by FastQC (version 0.11.5)