FastQCFastQC Report
Thu 26 May 2016
SRR1926560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646942
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC25300.3910706060203233No Hit
GTACATGGGAAGCAGTGGTATCAAC25130.38844285886524604No Hit
GTCCTACAGTGGACATTTCTAAATT11590.17915052663144454No Hit
GAGTACATGGGAAGCAGTGGTATCA11480.1774502196487475No Hit
CATGTACTCTGCGTTGATACCACTG9280.14344407999480635No Hit
GCGTTGATACCACTGCTTCCCATGT8700.13447882499513095No Hit
CTGTAGGACGTGGAATATGGCAAGA8480.13107821102973682No Hit
ACGCAGAGTACATGGGAAGCAGTGG7550.11670288835784351No Hit
GTATCAACGCAGAGTACATGGGAAG7000.10820135344435822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAC501.17939235E-418.57471
GTCCTAC6200.018.189511
GACGTGC401.6721358E-417.7489227
GCTCTAG450.001245191217.690191
GTATCAC450.001245191217.690191
CCTAGAA703.732046E-617.4565182
TCCTACA7000.016.8746342
TTGGACT452.798134E-416.5918664
TCCAACG1200.015.68978618
TGTAGGA6100.015.1353432
ACCTACA751.311128E-414.4824462
GTCTATA658.7060605E-414.2882291
CCTACAG7750.014.1678713
CTGTAGG6100.014.137651
GTCCTAA2300.013.8444961
GATATAC2500.013.7983481
ACGTGCT500.001263883813.6021028
CTCTAGA904.0325343E-513.5772922
CCAGTAC652.5690405E-413.3801673
GTCATAT600.00845524113.2676411
Produced by FastQC (version 0.11.5)