##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926560_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 646942 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.91365068275054 27.0 7.0 30.0 2.0 31.0 2 16.37008108918574 16.0 2.0 30.0 2.0 31.0 3 19.86862655384872 23.0 15.0 31.0 2.0 31.0 4 22.045728983432827 28.0 2.0 35.0 2.0 37.0 5 22.2876888500051 28.0 2.0 35.0 2.0 35.0 6 23.273625765524574 30.0 2.0 35.0 2.0 37.0 7 23.17262443928513 30.0 7.0 35.0 2.0 37.0 8 23.591018978517393 31.0 8.0 35.0 2.0 37.0 9 26.13735698099675 31.0 17.0 37.0 2.0 39.0 10 26.807044526402677 32.0 23.0 37.0 2.0 39.0 11 23.92553119135873 30.0 7.0 37.0 2.0 39.0 12 24.96070281416263 31.0 10.0 37.0 2.0 39.0 13 21.679133523561617 27.0 2.0 35.0 2.0 39.0 14 24.992790698393364 30.0 16.0 38.0 2.0 40.0 15 26.081313317113434 31.0 17.0 38.0 2.0 40.0 16 26.442934606193447 31.0 17.0 38.0 2.0 40.0 17 25.730612636063203 31.0 10.0 38.0 2.0 40.0 18 25.794868782672946 31.0 11.0 38.0 2.0 40.0 19 26.191913649137017 31.0 16.0 38.0 2.0 40.0 20 26.441693382096076 31.0 15.0 39.0 2.0 40.0 21 24.248187627329806 30.0 2.0 37.0 2.0 40.0 22 25.212841336626777 31.0 2.0 38.0 2.0 40.0 23 25.196693057492016 31.0 2.0 38.0 2.0 40.0 24 24.292514630368718 31.0 2.0 38.0 2.0 40.0 25 24.065608663527797 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.6140868374333515 1101 2 9.501080599679739 1101 3 6.244312980875037 1101 4 7.667185886974913 1101 5 7.205688755142678 1101 6 6.948009227387324 1101 7 6.810510498121683 1101 8 5.079622810121759 1101 9 5.008918732579865 1101 10 4.708516416337574 1101 11 7.294669125494149 1101 12 5.013701855103751 1101 13 9.749348983609256 1101 14 6.865918067069533 1101 15 6.209762111708642 1101 16 5.634115298182618 1101 17 1.5810171607787353 1101 18 3.9475811684044473 1101 19 4.738326522282048 1101 20 2.5943082589339603 1101 21 0.07452817012621793 1101 22 3.8967013714039425 1101 23 5.019747180720284 1101 24 6.287867022081009 1101 25 6.784305917435017 1102 1 5.3018640344765835 1102 2 9.944446537109737 1102 3 6.477516893366637 1102 4 7.680909473923318 1102 5 7.371678451252588 1102 6 6.559306173553228 1102 7 6.5069601359922125 1102 8 6.55609559441206 1102 9 5.515306397778101 1102 10 4.879307127903317 1102 11 7.381632464551867 1102 12 5.080359810752224 1102 13 9.484647273760825 1102 14 6.897083601303834 1102 15 4.48129201538967 1102 16 5.311226475663389 1102 17 6.118495694341046 1102 18 6.36317513540213 1102 19 4.819025394827406 1102 20 5.4083790421789395 1102 21 3.9348059271634774 1102 22 5.131880196163145 1102 23 5.647026356409079 1102 24 6.98152788969842 1102 25 6.887494150093868 1103 1 4.511722145486761 1103 2 8.921071532833686 1103 3 5.8939422638630035 1103 4 7.607768797432495 1103 5 6.698400180831101 1103 6 6.387799520012468 1103 7 6.791372872172953 1103 8 6.385494324138811 1103 9 5.125010190733445 1103 10 4.259792355560457 1103 11 4.924518060139739 1103 12 3.8197759107624876 1103 13 8.20088449786029 1103 14 6.430060153321563 1103 15 5.37026283559393 1103 16 5.036568464997959 1103 17 2.79656314577457 1103 18 1.321978296261598 1103 19 3.7397157970806028 1103 20 4.2777590712941205 1103 21 6.475155537377354 1103 22 1.6520361866303936 1103 23 4.024736139641622 1103 24 5.448473038372082 1103 25 1.153695075242009 1104 1 5.390489866892942 1104 2 10.252354349734674 1104 3 7.054296427251554 1104 4 7.312249830839082 1104 5 7.0566665908662785 1104 6 7.054009549670258 1104 7 7.0251809638205245 1104 8 7.015977790125568 1104 9 5.8936637628305135 1104 10 4.584853524287709 1104 11 7.820284759146347 1104 12 4.498597772364931 1104 13 8.967671500931964 1104 14 6.575772600272 1104 15 6.09779223051385 1104 16 5.743478349909029 1104 17 6.0593399710859295 1104 18 3.3844789021694446 1104 19 4.219333802946537 1104 20 3.328339139498837 1104 21 2.39619437290078 1104 22 4.502412371506487 1104 23 4.778935174216031 1104 24 5.567172062879099 1104 25 4.303497866221328 1105 1 3.2757641431784563 1105 2 9.273096154746142 1105 3 6.100054414582573 1105 4 5.681508287737991 1105 5 1.7702863044859924 1105 6 4.724258401941583 1105 7 5.790534711196742 1105 8 2.801025701016176 1105 9 3.773204446865577 1105 10 3.2435079784477807 1105 11 6.429976191309688 1105 12 3.9546623070811506 1105 13 4.741443047737217 1105 14 3.597314354398023 1105 15 4.073728653641286 1105 16 4.2519706067158936 1105 17 5.011338534320448 1105 18 2.2013085145046745 1105 19 2.1620846850232596 1105 20 1.082990480667032 1105 21 2.5360073688935536 1105 22 3.878677305074792 1105 23 1.04743446518723 1105 24 4.161606841583541 1105 25 5.1144255153512255 1106 1 6.199960118876579 1106 2 11.021601440590484 1106 3 7.400732563329392 1106 4 8.944890113351576 1106 5 8.33226909994518 1106 6 7.498172899461228 1106 7 7.207211596543157 1106 8 6.938792083417752 1106 9 5.885528174639951 1106 10 5.154824701781671 1106 11 7.2101779637861405 1106 12 5.017037128254255 1106 13 4.268251289566919 1106 14 5.10579585858768 1106 15 5.23270529931321 1106 16 5.241989743516143 1106 17 5.614504467100115 1106 18 5.577824130505267 1106 19 5.684132880032827 1106 20 4.910751846625821 1106 21 5.0254338222685035 1106 22 5.78366216284935 1106 23 6.166517385128518 1106 24 6.977224700119855 1106 25 5.846428562375731 1107 1 4.00502224140207 1107 2 -13.726038044388059 1107 3 -5.537607923079763 1107 4 1.5753431884694429 1107 5 4.465091499291187 1107 6 4.934394243111907 1107 7 4.505477239932375 1107 8 5.2049964429179525 1107 9 3.4129417210856126 1107 10 2.6708553437261813 1107 11 5.479640320284162 1107 12 2.4792928489530723 1107 13 4.364192711866192 1107 14 3.612421086746135 1107 15 3.390238580496039 1107 16 2.976720569396889 1107 17 3.6405966325440637 1107 18 -1.5037519422799228 1107 19 1.3340655776938064 1107 20 1.696171552468794 1107 21 -1.7571408621857145 1107 22 1.4105626610980764 1107 23 -1.3705383665787032 1107 24 2.0117934365977206 1107 25 2.4632238434598968 1108 1 5.554587054371307 1108 2 10.703359148014915 1108 3 0.5629976370633685 1108 4 6.136919543149247 1108 5 7.246401711237237 1108 6 7.799913382798874 1108 7 7.715885660825148 1108 8 7.122733280527221 1108 9 5.8296866929431985 1108 10 4.7416337447816375 1108 11 7.16361480702923 1108 12 2.508199328826265 1108 13 3.7050243604514783 1108 14 4.75597111189871 1108 15 4.719115636600524 1108 16 4.729992777223611 1108 17 0.08074773783369693 1108 18 3.3722316183757535 1108 19 4.378139535760191 1108 20 4.832218516600268 1108 21 4.363107436456048 1108 22 1.2606105933664615 1108 23 4.3931792791401385 1108 24 4.876463083607412 1108 25 5.681054647298275 1109 1 3.6509763512074365 1109 2 -13.726038044388059 1109 3 -5.370029679384839 1109 4 2.432400725594878 1109 5 1.1012637439410966 1109 6 2.5199372900543793 1109 7 4.114977998083056 1109 8 4.324920463197174 1109 9 3.818080081713866 1109 10 2.8313228375479618 1109 11 1.0571198771589359 1109 12 1.4417482991808193 1109 13 2.7627344879894586 1109 14 3.614443320843307 1109 15 3.6385370448854104 1109 16 3.455654009305402 1109 17 4.158474169879057 1109 18 4.383700476841941 1109 19 2.768393113756485 1109 20 1.478447591165164 1109 21 0.8159941095913226 1109 22 3.1920932722738016 1109 23 3.448488895412126 1109 24 4.522655273552665 1109 25 5.039726710661537 1110 1 3.4596451760168776 1110 2 9.249483261142313 1110 3 6.2745709829341365 1110 4 7.715082993479513 1110 5 8.518344532145306 1110 6 3.803520391085712 1110 7 6.388332923863494 1110 8 7.379277065849589 1110 9 5.797087363586211 1110 10 5.643003255005613 1110 11 3.2348154137373086 1110 12 5.965843932949994 1110 13 10.418320355983518 1110 14 8.140395993218554 1110 15 7.385713676777712 1110 16 6.995818357635269 1110 17 7.584340147394588 1110 18 7.541171069598416 1110 19 6.296111914970705 1110 20 6.608304755392002 1110 21 4.3201003384806285 1110 22 6.736158476064574 1110 23 6.943169434358282 1110 24 7.738022991163788 1110 25 8.262499442028957 1111 1 5.273416272708314 1111 2 9.65911479403989 1111 3 7.648272717386028 1111 4 8.340163460803645 1111 5 8.596740490765505 1111 6 7.82154190446526 1111 7 7.70719098069144 1111 8 7.954302700581383 1111 9 6.434464584108248 1111 10 5.557321094007619 1111 11 7.228286178265964 1111 12 6.774167401695415 1111 13 8.54866435626857 1111 14 6.235409592493262 1111 15 5.87354717573055 1111 16 5.62631794787546 1111 17 6.910300707518427 1111 18 6.75009393577918 1111 19 5.558428661279901 1111 20 5.151687431460051 1111 21 7.911394427250045 1111 22 7.453081358081462 1111 23 7.544612105596311 1111 24 7.36405679337847 1111 25 7.8499857338808745 1112 1 -1.7735541541825484 1112 2 7.9020758346154985 1112 3 6.551656608355586 1112 4 0.20961908559967668 1112 5 4.591637847367782 1112 6 3.3029130213435316 1112 7 6.181968366509402 1112 8 6.479990745461645 1112 9 5.095929408736449 1112 10 4.040198061792669 1112 11 0.22170332538557957 1112 12 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2204 8 5.449146958586251 2204 9 3.8342468165693653 2204 10 3.445939492690318 2204 11 4.956203594702448 2204 12 4.895169743651582 2204 13 3.2082165192350978 2204 14 4.25745683658775 2204 15 4.434140420352264 2204 16 4.411159798975952 2204 17 -1.0903464823251987 2204 18 3.0888248476902973 2204 19 3.7207693948890714 2204 20 3.9130776288644142 2204 21 5.375536518658922 2204 22 4.879579465032069 2204 23 3.4489410950403183 2204 24 5.332705908745599 2204 25 5.288056084257384 2205 1 5.434569382882572 2205 2 9.844402750771838 2205 3 6.708574733215251 2205 4 7.140253288056023 2205 5 7.59858534626818 2205 6 6.85176235865902 2205 7 6.931836106430723 2205 8 6.865588853219776 2205 9 5.545349156088939 2205 10 4.8942744404501966 2205 11 7.398504292422061 2205 12 6.365494920160955 2205 13 9.359201319428074 2205 14 7.137570353080989 2205 15 5.937895947022064 2205 16 5.578396241006693 2205 17 6.481984742045309 2205 18 6.183960319628387 2205 19 4.712851521183353 2205 20 5.1641213348315915 2205 21 -1.2252403721294698 2205 22 3.730588689570446 2205 23 5.198987032470136 2205 24 6.866221328220497 2205 25 7.164344884371719 2206 1 3.8531790029967503 2206 2 8.191558522135548 2206 3 5.641075910968876 2206 4 1.5165639330940657 2206 5 5.226689700803551 2206 6 5.426069895169384 2206 7 5.705065673796376 2206 8 5.452096804447795 2206 9 4.2769444836528265 2206 10 3.4312195515670787 2206 11 2.8694054315973005 2206 12 4.189569317095458 2206 13 7.421899358236445 2206 14 -0.46416122295738305 2206 15 1.5949370589698617 2206 16 2.4007880267779456 2206 17 3.6514143383447504 2206 18 -1.4359097750741299 2206 19 0.6103131375435566 2206 20 1.9107518466258213 2206 21 2.9121291012384596 2206 22 -2.785436549597005 2206 23 -3.375971885257748 2206 24 0.7188556014074088 2206 25 2.093638862804916 2207 1 1.0619942575280774 2207 2 -13.726038044388059 2207 3 -6.387637214233836 2207 4 -4.546987059913771 2207 5 0.694357546659166 2207 6 1.1645592258979427 2207 7 2.362038602735783 2207 8 2.5242714652109726 2207 9 -0.1772851338524717 2207 10 -0.25821677600105275 2207 11 -1.813424169313869 2207 12 -3.0078450157073675 2207 13 -0.16922806205515784 2207 14 -5.856681795907441 2207 15 -1.930799682697245 2207 16 -0.9450706063182928 2207 17 0.3372874535909851 2207 18 0.15922663621892852 2207 19 -0.29223556999998124 2207 20 -0.49511266984385216 2207 21 -4.244756331378056 2207 22 -4.655261632379084 2207 23 -1.9955740787000487 2207 24 -0.11610068814507812 2207 25 0.5542919172562897 2208 1 4.925742852993231 2208 2 6.235437365448007 2208 3 6.327419399324608 2208 4 8.202376217622312 2208 5 3.9125934508914995 2208 6 6.439395723636281 2208 7 6.794867263889952 2208 8 6.853268267193169 2208 9 5.268754798153633 2208 10 4.588135755042771 2208 11 6.658728586455528 2208 12 6.339361760633064 2208 13 8.710504860241652 2208 14 3.0518030351162118 2208 15 4.705983284256572 2208 16 4.949036109519099 2208 17 5.929350031020469 2208 18 0.6842302375903309 2208 19 3.274142026588798 2208 20 4.188501090276002 2208 21 6.96402525264914 2208 22 5.831544383351705 2208 23 1.8033815235933055 2208 24 3.1868250659820916 2208 25 4.728846067476695 2209 1 3.5268954018568834 2209 2 5.280251263788042 2209 3 5.78000213981332 2209 4 0.843119004065688 2209 5 5.259790510971328 2209 6 5.94441685983589 2209 7 6.464209464116777 2209 8 5.398787687457407 2209 9 4.901865722696666 2209 10 4.176416579280911 2209 11 0.0711915796948901 2209 12 3.7552013906391046 2209 13 2.7336508399450103 2209 14 4.143945082160826 2209 15 4.87725720056606 2209 16 4.41265017871622 2209 17 1.8673215450204417 2209 18 4.194578137962974 2209 19 4.658956366800602 2209 20 4.791672993123129 2209 21 7.0391846947123895 2209 22 5.87158753949446 2209 23 1.1118794541605226 2209 24 -2.166351992035672 2209 25 1.0300671064645144 2210 1 -2.0413950261546283 2210 2 -13.726038044388059 2210 3 -7.627946087330972 2210 4 -2.284750574173046 2210 5 -0.8814233385279984 2210 6 -1.3601132650387804 2210 7 -1.1728883391828298 2210 8 -20.849190749200275 2210 9 -12.959438755342841 2210 10 -7.101620323202262 2210 11 -9.524826493927778 2210 12 -4.6897821373632915 2210 13 -5.200372624513237 2210 14 -4.053479296395629 2210 15 -5.6272312560142765 2210 16 -5.063456960885588 2210 17 -4.466207108442585 2210 18 -4.919163284227995 2210 19 -10.10770639346347 2210 20 -7.347061592863302 2210 21 -5.518274581040572 2210 22 -10.10073169172551 2210 23 -8.142690385807185 2210 24 -10.684130030240288 2210 25 -8.351663950738242 2211 1 0.3404128025039199 2211 2 4.9317566324180255 2211 3 0.9018724317998306 2211 4 1.7926772835077465 2211 5 1.185578553428428 2211 6 0.2913810131706569 2211 7 -20.466608145946115 2211 8 -13.422384665550085 2211 9 -8.969788491754738 2211 10 -7.055204019313649 2211 11 -9.706175109860624 2211 12 -4.833043649826326 2211 13 -6.893951912182459 2211 14 -4.6877843238565475 2211 15 -5.065378057138428 2211 16 -11.100914024495735 2211 17 -7.650438661867394 2211 18 -5.821314236635189 2211 19 -8.395156109670005 2211 20 -6.658665898441818 2211 21 -4.275468607601436 2211 22 -5.923666612098025 2211 23 -8.124462395873127 2211 24 -21.71891263893594 2211 25 -21.507219506293797 2212 1 -0.9329557083985378 2212 2 -13.726038044388059 2212 3 -7.645431052331464 2212 4 -19.434509028279326 2212 5 -19.55871802451834 2212 6 -20.514702637019457 2212 7 -9.810485696966524 2212 8 -9.157354014506398 2212 9 -5.0125098388487075 2212 10 -5.3911549776437475 2212 11 -5.393864253959329 2212 12 -2.8582628629868765 2212 13 -5.9180498403271 2212 14 -3.6360189789353115 2212 15 -3.7871247786407167 2212 16 -3.86079995605467 2212 17 -4.097381317256545 2212 18 -3.1019691602015733 2212 19 -3.564198555545694 2212 20 -3.6307695688073025 2212 21 -1.4682597925164664 2212 22 -3.067117376434176 2212 23 -3.1640992392036154 2212 24 -2.059728965466551 2212 25 -2.4817093022121632 2213 1 -18.355404687990376 2213 2 -13.726038044388059 2213 3 -8.856564259607985 2213 4 -5.883923298779859 2213 5 -19.55871802451834 2213 6 -10.84590604490018 2213 7 -20.466608145946115 2213 8 -11.017454859956398 2213 9 -8.529366677430787 2213 10 -7.574568726529883 2213 11 -6.898455547345691 2213 12 -5.600183273323534 2213 13 -18.88539677910261 2213 14 -12.481736307400244 2213 15 -7.40432066658056 2213 16 -6.706327112604193 2213 17 -5.3614703394468926 2213 18 -5.3420769605101945 2213 19 -6.1953389350201675 2213 20 -6.111670875016689 2213 21 -4.254756499825589 2213 22 -5.115400395712175 2213 23 -4.841173199778261 2213 24 -4.033076643195791 2213 25 -4.724471902459932 2214 1 -18.355404687990376 2214 2 -13.726038044388059 2214 3 -11.615916130784957 2214 4 -19.434509028279326 2214 5 -19.55871802451834 2214 6 -20.514702637019457 2214 7 -15.810829251473752 2214 8 -14.262507332114849 2214 9 -12.684297347822145 2214 10 -12.404927924008224 2214 11 -13.245110280007937 2214 12 -10.971635850372785 2214 13 -18.88539677910261 2214 14 -15.83569593514493 2214 15 -23.505492125579494 2214 16 -17.398237141984318 2214 17 -23.219830296848382 2214 18 -16.774320713888372 2214 19 -13.61000411394996 2214 20 -12.227785259505694 2214 21 -9.737770611919604 2214 22 -10.756667166199328 2214 23 -10.685184256124959 2214 24 -9.501575955518854 2214 25 -9.223299908303847 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 117797.0 3 3.0 4 520.0 5 2155.0 6 2815.0 7 2892.0 8 2464.0 9 2435.0 10 2247.0 11 2093.0 12 1940.0 13 2136.0 14 3108.0 15 6564.0 16 9570.0 17 10099.0 18 10496.0 19 12173.0 20 14500.0 21 16836.0 22 17568.0 23 19363.0 24 20582.0 25 20970.0 26 17713.0 27 14549.0 28 15507.0 29 17684.0 30 17052.0 31 19897.0 32 25355.0 33 31601.0 34 37911.0 35 42126.0 36 40398.0 37 40366.0 38 25457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.986710256269255 18.03339464968966 12.688405887079751 25.291489206961327 2 12.846089688275915 19.81535473667259 42.21242617915061 25.126129395900886 3 20.94686882781255 24.33893108768068 27.779142551121677 26.935057533385088 4 11.674103518841314 16.69339889419569 36.734184125874 34.898313461088996 5 12.24572325538085 37.76429793989057 35.688048172683814 14.301930632044769 6 32.18167869646022 35.85040555531544 18.39369840515192 13.574217343072423 7 27.409619621813263 30.7452123584263 21.941863684933857 19.903304334826576 8 24.54537271936287 39.529821216050884 19.749409109547255 16.175396955038988 9 26.444920342151196 16.150830001361673 19.363913199559313 38.04033645692782 10 14.998525295882123 30.35364659813999 33.62372453782145 21.024103568156434 11 33.17352970562173 22.73491870324843 23.71781747663138 20.373734114498454 12 24.678862095074518 25.139714245444743 31.09735911395766 19.08406454552308 13 29.07098731586066 21.394707021968784 24.163468216544214 25.37083744562635 14 20.072667637453524 24.06121926430693 26.769369004252297 29.096744093987247 15 24.173101632170162 31.50387370081545 22.481299375373695 21.841725291640692 16 20.584338876374403 28.1365529841074 30.211088314019573 21.068019825498627 17 21.52655785241557 28.721843239436396 29.385411980166065 20.366186927981968 18 21.37842911949567 27.078035426124416 31.848035614494446 19.69549983988547 19 23.137993253314505 27.288460029810935 26.839883894249628 22.733662822624932 20 23.262533599129444 28.417655393067186 27.910451925730033 20.409359082073333 21 21.16123049982796 26.77514769002622 26.802771419294285 25.260850390851537 22 21.891404805188888 29.391440158256042 26.73304663305245 21.984108403502624 23 20.88130935267384 28.7546634934537 28.172529528366262 22.191497625506194 24 22.559823490834773 28.68070583330933 27.899157955028798 20.8603127208271 25 22.26940235800954 27.369963619369784 27.642315423623113 22.71831859899756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1091.0 1 1091.0 2 1893.5 3 2696.0 4 2696.0 5 2696.0 6 3583.5 7 4471.0 8 4471.0 9 4471.0 10 5031.0 11 5591.0 12 5591.0 13 5591.0 14 6505.5 15 7420.0 16 7420.0 17 7420.0 18 10126.0 19 12832.0 20 12832.0 21 12832.0 22 17839.0 23 22846.0 24 22846.0 25 22846.0 26 30984.0 27 39122.0 28 39122.0 29 39122.0 30 47891.5 31 56661.0 32 56661.0 33 56661.0 34 62368.0 35 68075.0 36 68075.0 37 68075.0 38 70479.0 39 72883.0 40 72883.0 41 72883.0 42 76160.5 43 79438.0 44 79438.0 45 79438.0 46 83757.5 47 88077.0 48 88077.0 49 88077.0 50 79607.0 51 71137.0 52 71137.0 53 71137.0 54 58943.5 55 46750.0 56 46750.0 57 46750.0 58 38946.5 59 31143.0 60 31143.0 61 31143.0 62 25119.0 63 19095.0 64 19095.0 65 19095.0 66 14743.0 67 10391.0 68 10391.0 69 10391.0 70 7393.0 71 4395.0 72 4395.0 73 4395.0 74 3120.5 75 1846.0 76 1846.0 77 1846.0 78 1268.5 79 691.0 80 691.0 81 691.0 82 453.0 83 215.0 84 215.0 85 215.0 86 137.0 87 59.0 88 59.0 89 59.0 90 37.0 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.86034915031023 2 35.69114387379394 3 5.617195977382826 4 13.362094283567924 5 12.8507656018623 6 14.167266926556014 7 9.010235848035835 8 7.264948016978338 9 0.10480073947896412 10 1.4724658470156522 11 5.626624952468691 12 5.683353376345948 13 15.426730680648342 14 1.5990614305455513 15 2.79561382627809 16 3.0403034584244026 17 2.769800074813507 18 1.5299671377032253 19 1.4826676889118344 20 0.2837966927483453 21 4.313987961826562 22 1.62379316847569 23 0.10804678008229486 24 8.784558739423318 25 0.11113824732356223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 646942.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.89547158027804 #Duplication Level Percentage of deduplicated Percentage of total 1 96.32353008926296 83.70078571383607 2 2.454334518936583 4.265411108774984 3 0.5039212212682499 1.3136541648424263 4 0.2231137712976476 0.7755030549185357 5 0.12137031361361383 0.5273265318650607 6 0.06934055388281754 0.36152280775710655 7 0.051966638754436564 0.31609659067061024 8 0.03670808888003729 0.25518133552332806 9 0.030098561283573858 0.23538858089616443 >10 0.15383658155176003 2.5267821151596306 >50 0.014291531479787204 0.8382600142635739 >100 0.015043552506492254 2.632407406600519 >500 0.0016923996568545094 1.0506753335671661 >1k 7.521776252686709E-4 1.2010052413248322 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 2530 0.3910706060203233 No Hit GTACATGGGAAGCAGTGGTATCAAC 2513 0.38844285886524604 No Hit GTCCTACAGTGGACATTTCTAAATT 1159 0.17915052663144454 No Hit GAGTACATGGGAAGCAGTGGTATCA 1148 0.1774502196487475 No Hit CATGTACTCTGCGTTGATACCACTG 928 0.14344407999480635 No Hit GCGTTGATACCACTGCTTCCCATGT 870 0.13447882499513095 No Hit CTGTAGGACGTGGAATATGGCAAGA 848 0.13107821102973682 No Hit ACGCAGAGTACATGGGAAGCAGTGG 755 0.11670288835784351 No Hit GTATCAACGCAGAGTACATGGGAAG 700 0.10820135344435822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.545733620633689E-4 0.0 0.0 1.545733620633689E-4 13 0.0 1.545733620633689E-4 0.0 0.0 3.091467241267378E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCTAC 50 1.17939235E-4 18.5747 1 GTCCTAC 620 0.0 18.18951 1 GACGTGC 40 1.6721358E-4 17.748922 7 GCTCTAG 45 0.0012451912 17.69019 1 GTATCAC 45 0.0012451912 17.69019 1 CCTAGAA 70 3.732046E-6 17.456518 2 TCCTACA 700 0.0 16.874634 2 TTGGACT 45 2.798134E-4 16.591866 4 TCCAACG 120 0.0 15.689786 18 TGTAGGA 610 0.0 15.135343 2 ACCTACA 75 1.311128E-4 14.482446 2 GTCTATA 65 8.7060605E-4 14.288229 1 CCTACAG 775 0.0 14.167871 3 CTGTAGG 610 0.0 14.13765 1 GTCCTAA 230 0.0 13.844496 1 GATATAC 250 0.0 13.798348 1 ACGTGCT 50 0.0012638838 13.602102 8 CTCTAGA 90 4.0325343E-5 13.577292 2 CCAGTAC 65 2.5690405E-4 13.380167 3 GTCATAT 60 0.008455241 13.267641 1 >>END_MODULE