FastQCFastQC Report
Thu 26 May 2016
SRR1926560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646942
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC66131.0221936433250585No Hit
GTACATGGGAAGCAGTGGTATCAAC65121.0065817337566583No Hit
GAGTACATGGGAAGCAGTGGTATCA30960.4785591289481901No Hit
CATGTACTCTGCGTTGATACCACTG24110.37267637593478237No Hit
CTGTAGGACGTGGAATATGGCAAGA22620.34964494498734044No Hit
GTCCTACAGTGGACATTTCTAAATT22180.3428437170565522No Hit
GCGTTGATACCACTGCTTCCCATGT20660.31934856602292017No Hit
ACGCAGAGTACATGGGAAGCAGTGG19600.30296378964420306No Hit
GTATCAACGCAGAGTACATGGGAAG18190.281168945593268No Hit
GTATCAACGCAGAGTACTTTTTTTT15160.23433321688806724No Hit
CTTTAGGACGTGAAATATGGCGAGG15110.2335603500777504No Hit
GTCCTAAAGTGTGTATTTCTCATTT14640.22629540206077206No Hit
ACTCTGCGTTGATACCACTGCTTCC14560.22505881516426512No Hit
TATCAACGCAGAGTACATGGGAAGC12430.19213468904476755No Hit
GGTATCAACGCAGAGTACATGGGAA12060.1864154746484229No Hit
GTCCTACAGTGTGCATTTCTCATTT11850.18316943404509214No Hit
GCTTCCCATGTACTCTGCGTTGATA11510.1779139397349376No Hit
GATATACACTGTTCTACAAATCCCG10620.16415691051129777No Hit
CTGTAGGACCTGGAATATGGCGAGA10050.15534622887368574No Hit
GGTATCAACGCAGAGTACTTTTTTT9950.15380049525305206No Hit
CTGAAGGACCTGGAATATGGCGAGA9480.1465355472360737No Hit
GTGTATATCAATGAGTTACAATGAA9200.1422074930982994No Hit
CAGTGGTATCAACGCAGAGTACATG9170.14174377301210928No Hit
TATCAACGCAGAGTACTTTTTTTTT8410.12999619749529326No Hit
CATGGGAAGCAGTGGTATCAACGCA7670.11855776870260396No Hit
GATATACACTGTTCTACAATGCCGG7550.11670288835784351No Hit
GTGGTATCAACGCAGAGTACATGGG7410.11453886128895635No Hit
ACATGGGAAGCAGTGGTATCAACGC7360.1137659944786395No Hit
GCAGTGGTATCAACGCAGAGTACAT7340.11345684775451277No Hit
ATACCACTGCTTCCCATGTACTCTG7240.11191111413387908No Hit
GTGTATATCAATGAGTTACAATGAG7100.10974708706499192No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7100.10974708706499192No Hit
GTTCTACAGTGTGGTTTTTATCATT6490.1003181119791264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3500.014.7074674
CCAACGA1152.5465852E-1114.00891219
TCGTCAA500.00145541313.3508552
GGACCAT500.001459685613.3456646
TAAGCTG500.001459685613.3456645
CTACACT1153.5470293E-1013.2627724
TCCAACG1800.013.16298718
CCAACGT655.57019E-513.1214719
TAGCACT603.969766E-412.7101574
AAAGTAC853.7754307E-612.3392073
GAACTGT701.0546891E-412.2562246
CTATAAG701.0569891E-412.2533651
TGTAGGA10700.012.2100612
ACGTTTT550.00312220712.0611519
GTCCTAA4600.012.0165841
CTGTAGG10750.011.7910931
GTAGGAC10850.011.7757493
GGACGTG9650.011.7552496
AGGACGT9700.011.6946555
AGGACCT6250.011.5916645
Produced by FastQC (version 0.11.5)