FastQCFastQC Report
Thu 26 May 2016
SRR1926559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660387
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC29770.45079627551723456No Hit
CCCATGTACTCTGCGTTGATACCAC26700.4043083828118967No Hit
GAGTACATGGGAAGCAGTGGTATCA12780.19352288885153704No Hit
CATGTACTCTGCGTTGATACCACTG10450.1582405468308734No Hit
GCGTTGATACCACTGCTTCCCATGT9600.14536930617955834No Hit
GTCCTACAGTGGACATTTCTAAATT9530.1443093216553324No Hit
GTATCAACGCAGAGTACATGGGAAG8220.12447246841624685No Hit
ACGCAGAGTACATGGGAAGCAGTGG8020.1214439412041727No Hit
CTGTAGGACGTGGAATATGGCAAGA6690.10130423524387974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAT350.00557486919.2562471
TTAGTAC300.007254320718.3987833
TAGGACC2050.016.3031884
CTTGGTA350.00263917715.8125519
ATGTAGG707.746885E-515.4049971
GTTCTGA801.4214054E-515.1642931
TGTAGGA5500.015.0902722
CTAGGAC1206.730261E-1114.7190273
GTCCAAG550.004276925714.7047691
CTGTAGG4850.014.4521091
GTCCTAC5500.014.214611
TCCGTTG459.705466E-414.08637112
GAACAGT1950.013.974796
CTCCGTT400.00641129513.83598211
CTACACT1106.4555934E-913.7214514
GCGGGGG400.007243534613.58328812
GTCCTAG1202.5208465E-713.4793721
GTATTGT802.4112532E-413.4793711
TATAACA652.756883E-413.2690974
CTTAGAC755.0868053E-513.2471243
Produced by FastQC (version 0.11.5)