FastQCFastQC Report
Thu 26 May 2016
SRR1926559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660387
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC79501.2038395667994675No Hit
GTACATGGGAAGCAGTGGTATCAAC69711.0555931597684387No Hit
GAGTACATGGGAAGCAGTGGTATCA33780.511518246119321No Hit
CATGTACTCTGCGTTGATACCACTG28330.42899087959030086No Hit
GCGTTGATACCACTGCTTCCCATGT23250.35206628840361787No Hit
ACGCAGAGTACATGGGAAGCAGTGG20830.31542110913752086No Hit
GTATCAACGCAGAGTACATGGGAAG19480.29497855045602045No Hit
CTGTAGGACGTGGAATATGGCAAGA19210.2908900387197204No Hit
GTCCTACAGTGGACATTTCTAAATT18230.27605025538055716No Hit
ACTCTGCGTTGATACCACTGCTTCC16080.24349358785076022No Hit
GCTTCCCATGTACTCTGCGTTGATA13260.20079135416051497No Hit
TATCAACGCAGAGTACATGGGAAGC12320.18655727626376656No Hit
CTTTAGGACGTGAAATATGGCGAGG12010.18186305908505165No Hit
GTATCAACGCAGAGTACTTTTTTTT11990.18156020636384423No Hit
GTCCTACAGTGTGCATTTCTCATTT11710.17732026826694045No Hit
GTCCTAAAGTGTGTATTTCTCATTT11600.17565457830029965No Hit
GGTATCAACGCAGAGTACATGGGAA11390.17247462472762182No Hit
CAGTGGTATCAACGCAGAGTACATG10770.16308619037019204No Hit
CTGTAGGACCTGGAATATGGCGAGA10360.15687770958544006No Hit
GATATACACTGTTCTACAAATCCCG9580.14506645345835092No Hit
CATGGGAAGCAGTGGTATCAACGCA8810.13340662369186554No Hit
GGTATCAACGCAGAGTACTTTTTTT7860.1190211194345134No Hit
GCAGTGGTATCAACGCAGAGTACAT7760.11750685582847632No Hit
ATACCACTGCTTCCCATGTACTCTG7640.11568973950123185No Hit
CTGAAGGACCTGGAATATGGCGAGA7350.11129837504372438No Hit
GTGGTATCAACGCAGAGTACATGGG7290.11038981688010212No Hit
GTGTATATCAATGAGTTACAATGAA7170.10857270055285764No Hit
ACATGGGAAGCAGTGGTATCAACGC7030.10645273150440575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC3350.015.3715461
CCAACGA851.7753337E-814.48847519
TAGTACC400.005138694314.3062584
ACTGTTC3750.013.6839378
TCTAGGA852.5554255E-713.4688212
CACTGTT3750.013.4305317
TAGGAGT500.00145411113.3525084
GTACCCA500.001455783113.3504726
TAGGACC4150.013.3295264
TATACAC4500.012.5047293
TGTAGGA9550.012.4874532
TCCAACG1600.012.43361918
AGGACTC701.05046274E-412.2615735
AGGACCT6950.012.2125655
GTAGGAC9150.012.1954993
CTAGTCC550.00297605612.1386433
TAGGCTT550.00297775612.1377195
CTATTAT550.002979457412.1367931
GACAGTA550.00305861712.0943897
GCGTGCG550.00305861712.0943899
Produced by FastQC (version 0.11.5)