##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926559_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660387 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.291247404930743 31.0 28.0 31.0 22.0 34.0 2 28.74699986523054 31.0 28.0 33.0 23.0 34.0 3 29.144522832823785 31.0 28.0 33.0 25.0 34.0 4 32.884136120184074 35.0 33.0 37.0 27.0 37.0 5 32.55203994021687 35.0 33.0 37.0 25.0 37.0 6 32.68135653790883 35.0 33.0 37.0 26.0 37.0 7 32.483324172038515 35.0 33.0 37.0 24.0 37.0 8 32.48631787118765 35.0 33.0 37.0 25.0 37.0 9 33.66440435683925 37.0 33.0 39.0 22.0 39.0 10 33.56894366485106 37.0 33.0 39.0 22.0 39.0 11 33.82878069980178 37.0 34.0 39.0 25.0 39.0 12 33.61989409240339 37.0 33.0 39.0 23.0 39.0 13 33.76424884196691 37.0 34.0 39.0 25.0 39.0 14 34.395259143502216 38.0 33.0 40.0 19.0 41.0 15 34.528249344702424 38.0 34.0 40.0 23.0 41.0 16 34.47450358653335 38.0 34.0 40.0 22.0 41.0 17 34.41600152637771 38.0 34.0 40.0 22.0 41.0 18 34.37907015129008 38.0 34.0 40.0 19.0 41.0 19 34.3937509369506 39.0 34.0 40.0 19.0 41.0 20 34.400695349847894 39.0 34.0 40.0 19.0 41.0 21 34.303290343389556 39.0 34.0 40.0 18.0 41.0 22 34.25205220575208 39.0 34.0 40.0 18.0 41.0 23 33.90767231941271 39.0 34.0 40.0 15.0 41.0 24 34.00041793675527 39.0 34.0 40.0 16.0 41.0 25 33.77303459940914 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7375441274399748 1101 2 0.9170029917566076 1101 3 0.9019874807135437 1101 4 0.6455269994078279 1101 5 0.7084583278289927 1101 6 0.27944414296050013 1101 7 0.5452327301252708 1101 8 0.5984468763181354 1101 9 1.0606828422091752 1101 10 0.9735079010854406 1101 11 0.7788216345097752 1101 12 0.8836390059247563 1101 13 0.7160107660509851 1101 14 1.3471802398384725 1101 15 0.979904045210823 1101 16 1.1201937820451988 1101 17 0.902528964106935 1101 18 1.3159784189043506 1101 19 0.9489735935292174 1101 20 1.2462684869656755 1101 21 0.9790313169121916 1101 22 0.9944980065411215 1101 23 1.5299864699527674 1101 24 1.304115596180857 1101 25 1.4663521586327448 1102 1 0.33790453752418514 1102 2 0.6134050051616811 1102 3 0.4814578216571874 1102 4 0.39506595211991424 1102 5 0.505689234852774 1102 6 0.20860645804583555 1102 7 0.4163600981566802 1102 8 0.6581285388645739 1102 9 0.6479521533152948 1102 10 0.6398451695703002 1102 11 0.4754318628190646 1102 12 0.6378801452238321 1102 13 0.3248617172295454 1102 14 0.9164141742408631 1102 15 0.7354927976399992 1102 16 0.6564322641254918 1102 17 0.6728670985013991 1102 18 0.7125253564927618 1102 19 0.6247747251606981 1102 20 0.6913427172315281 1102 21 0.6434896937613388 1102 22 0.5549968299616665 1102 23 0.6486003866403678 1102 24 0.7809690393282693 1102 25 0.6395737389508227 1103 1 0.4217847637642578 1103 2 0.10554710630906072 1103 3 0.27839960326405944 1103 4 0.24736104845396056 1103 5 0.21998614461291766 1103 6 -0.05977891859400586 1103 7 0.3328944656147854 1103 8 0.35467965860462414 1103 9 0.785261035732475 1103 10 0.7680355186379657 1103 11 0.4261988611606924 1103 12 0.3848675995511144 1103 13 0.5285932290471536 1103 14 0.767912947313171 1103 15 0.686901067941605 1103 16 0.4724124917138397 1103 17 0.31092932800428486 1103 18 0.41528526966403234 1103 19 0.737909929895828 1103 20 0.5495954913015595 1103 21 0.6429655529477927 1103 22 0.6206854936890949 1103 23 0.7767477595320074 1103 24 0.7751866369768834 1103 25 0.8039685669787033 1104 1 0.5701393444461758 1104 2 0.7797133549098412 1104 3 0.7629263646816575 1104 4 0.5613817381146546 1104 5 0.4613369815048074 1104 6 0.10583918281877658 1104 7 0.3051973005415647 1104 8 0.35398274877163516 1104 9 0.534553524228663 1104 10 0.5536673342121006 1104 11 0.40681100563466543 1104 12 0.556801096270199 1104 13 0.32894256410235556 1104 14 0.6908472017516729 1104 15 0.6707809274074563 1104 16 0.4647464835509538 1104 17 0.39677165675529835 1104 18 0.6552166828547499 1104 19 0.5707627875061903 1104 20 0.5917069280639922 1104 21 0.7602536375499227 1104 22 0.6056583254073757 1104 23 0.8001370456835062 1104 24 0.4405194934350618 1104 25 0.5469544615556856 1105 1 0.6653097956003826 1105 2 0.5710703509395323 1105 3 0.7750991165305301 1105 4 0.6306484842034337 1105 5 0.7523275057623735 1105 6 0.3700951734982212 1105 7 0.19658716363790063 1105 8 0.2128549070237753 1105 9 0.7626239090916656 1105 10 0.5985456027145517 1105 11 0.5624672049762083 1105 12 0.8074224270760268 1105 13 0.8192722454509749 1105 14 1.1348963925294768 1105 15 0.9448771427607596 1105 16 0.957263854153787 1105 17 0.9837830376734615 1105 18 0.9612011151657072 1105 19 0.9189825941714389 1105 20 0.8763643744441154 1105 21 0.837873234279769 1105 22 0.8175410830895586 1105 23 1.0312784719550336 1105 24 0.733569581093839 1105 25 1.1058139663850781 1106 1 0.6096269758073163 1106 2 0.772303405738608 1106 3 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1.194348390476847 1107 16 1.1871033595527507 1107 17 0.8354561236598386 1107 18 1.109031199371394 1107 19 1.0771044846320734 1107 20 0.8392112556762541 1107 21 0.8948090016148385 1107 22 1.0254248899202665 1107 23 1.149386989800277 1107 24 1.2696930549092826 1107 25 1.3738095367062257 1108 1 1.3220717984889845 1108 2 1.0349098953863276 1108 3 1.0859545360375051 1108 4 0.7880222457371531 1108 5 -0.3745480174522555 1108 6 0.47054548305729327 1108 7 0.8635896076060234 1108 8 0.9540380697086448 1108 9 1.4304995273665355 1108 10 1.3134354094969467 1108 11 0.8567070143650923 1108 12 0.875727809632167 1108 13 0.8170137981220122 1108 14 1.17070267202498 1108 15 1.24368137692786 1108 16 1.2906109865543058 1108 17 1.106521140577705 1108 18 1.1161217092002147 1108 19 1.1602224863547264 1108 20 1.2496943040075479 1108 21 1.2923677437842613 1108 22 1.3313677484069828 1108 23 1.7510117513186856 1108 24 1.3922534873951022 1108 25 1.557382307031915 1109 1 0.6098177602193751 1109 2 -0.37352519043075105 1109 3 0.7582170759273552 1109 4 0.9738350129529323 1109 5 -0.5379115787686501 1109 6 0.5786762355122903 1109 7 0.9161209958343548 1109 8 0.8729030418011519 1109 9 1.4437756082428166 1109 10 -1.4172295650597988 1109 11 0.2707636425485802 1109 12 0.9056713785877051 1109 13 0.8496711088993578 1109 14 1.3607908917364213 1109 15 1.302293271803478 1109 16 1.2342415480308802 1109 17 1.2290830853541337 1109 18 1.0770789423540705 1109 19 1.1557098509756685 1109 20 1.2383280073872456 1109 21 1.218015530282706 1109 22 1.2038834887256087 1109 23 1.126370605940977 1109 24 1.0696632038845522 1109 25 1.236723369857657 1110 1 0.8835837740847445 1110 2 -0.1525753805205241 1110 3 0.6011150664068907 1110 4 1.018566810659074 1110 5 1.241875009519262 1110 6 0.7193282913571508 1110 7 1.1098368515578159 1110 8 1.3603475323925096 1110 9 1.6149825984348283 1110 10 1.2766236664663495 1110 11 1.2367200017703297 1110 12 1.3266052145673797 1110 13 1.2545655212184528 1110 14 1.6368523594211553 1110 15 1.3308434778671838 1110 16 1.1356440591327583 1110 17 1.2898942415472305 1110 18 1.514533017303748 1110 19 1.459337530602511 1110 20 1.448409154954419 1110 21 1.324004156844154 1110 22 1.4943294808664334 1110 23 1.6004545010242026 1110 24 1.5224794078396968 1110 25 1.6192990064187924 1111 1 0.04606981427359713 1111 2 0.2507965396563101 1111 3 0.24862232550868768 1111 4 0.20227176379261635 1111 5 0.33092743679345915 1111 6 0.1553203429295067 1111 7 0.2972372073885907 1111 8 0.3642909383553672 1111 9 0.6570052884230435 1111 10 0.5648188650615111 1111 11 0.04209674337711533 1111 12 -0.02059396594356855 1111 13 0.1610390226086551 1111 14 0.38076588176070914 1111 15 0.3700095091217577 1111 16 0.4945856551338963 1111 17 0.12137602254956192 1111 18 0.4494283090926885 1111 19 0.2233208327428926 1111 20 0.18828684775385085 1111 21 0.37536393872451157 1111 22 0.32973849370560515 1111 23 0.9021165850218296 1111 24 0.524326338991834 1111 25 0.41378362944612235 1112 1 -0.06532918773745067 1112 2 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0.2786094486862254 2202 23 0.4834070352156772 2202 24 0.23704095248973545 2202 25 0.3817447023158138 2203 1 0.11053425107343173 2203 2 0.15509505768458354 2203 3 0.022008896367204045 2203 4 -0.49822351974084 2203 5 -0.07293834452055137 2203 6 -0.19185814096468334 2203 7 0.1291341457460291 2203 8 0.01322630906648925 2203 9 0.2541334681893872 2203 10 0.17306001456537956 2203 11 -0.04764824499839193 2203 12 0.1726878268713108 2203 13 0.02847370603205235 2203 14 0.38791028006272654 2203 15 0.38918810938212545 2203 16 0.2317459810561644 2203 17 0.35695449577685423 2203 18 0.15992623510803838 2203 19 0.2516802393723694 2203 20 0.36995869029102124 2203 21 0.40372576964930573 2203 22 0.6202199829987052 2203 23 0.8320277830843352 2203 24 0.4684075431878014 2203 25 0.3798556164712963 2204 1 0.4284081844479388 2204 2 0.5694604181420289 2204 3 0.40575207437549565 2204 4 0.3642241205537644 2204 5 0.5471366921364904 2204 6 0.32228748728875445 2204 7 0.633862799581685 2204 8 0.37876742587747003 2204 9 0.7913530785533567 2204 10 0.8572187091124093 2204 11 0.5261485846398273 2204 12 0.6644140481076093 2204 13 0.5009836528005636 2204 14 1.091155006279763 2204 15 0.7677072907851326 2204 16 0.8423553072807621 2204 17 0.7990481277443209 2204 18 0.6482854699526115 2204 19 0.9823944320560827 2204 20 0.9502911875496238 2204 21 0.9377069558670996 2204 22 0.8782721389371488 2204 23 1.2095403137018934 2204 24 1.2823720919385266 2204 25 1.1098648174947527 2205 1 0.38490256249701105 2205 2 0.16644409479345157 2205 3 0.3175802733419282 2205 4 0.09436806500133343 2205 5 -0.040460456300692726 2205 6 -0.1727825560312084 2205 7 0.04078515316582809 2205 8 -0.0044334292698877675 2205 9 0.40786208412306735 2205 10 0.2509835275141512 2205 11 0.08408321087452464 2205 12 0.24184908825291984 2205 13 0.17720026869955063 2205 14 0.7059826506863445 2205 15 0.6382657888120278 2205 16 0.19445982171940557 2205 17 0.4032676048506474 2205 18 0.5734383757658463 2205 19 0.7294942447916739 2205 20 0.6856496019906828 2205 21 0.7388377757315965 2205 22 0.6779538698068421 2205 23 0.9155223266737735 2205 24 0.7452760566395824 2205 25 1.0698247927404836 2206 1 0.7365441242878745 2206 2 0.9482465890571525 2206 3 1.0353763960450344 2206 4 0.8255315069082485 2206 5 1.1935079260940782 2206 6 0.7889232902548926 2206 7 0.8735127247036587 2206 8 0.992807457438964 2206 9 1.2957418967688454 2206 10 1.300372603574651 2206 11 0.99579158555769 2206 12 1.122287512303032 2206 13 0.8985424682785705 2206 14 1.2976718095518507 2206 15 1.1313205981392827 2206 16 1.0274054675036197 2206 17 1.0781394467246912 2206 18 1.0412155354910055 2206 19 1.0792616322622024 2206 20 1.1278567662037489 2206 21 1.231614461382307 2206 22 0.8503273393365021 2206 23 1.3243420077164814 2206 24 1.1806676105292055 2206 25 1.16105884013529 2207 1 -1.106681712640917 2207 2 -0.18177892034353604 2207 3 -0.014814137132930227 2207 4 0.18759335933164323 2207 5 0.27638828385554604 2207 6 0.13171767188812566 2207 7 0.4204563205843641 2207 8 0.258577620888083 2207 9 0.5309118019746464 2207 10 0.5111369408026292 2207 11 0.3650385858411269 2207 12 0.2504990198771182 2207 13 0.2324416274131451 2207 14 -5.368457375092117 2207 15 -2.1726963451416665 2207 16 -0.9970993036991018 2207 17 -0.6924066434085248 2207 18 -0.17307760216527868 2207 19 0.07172691113348861 2207 20 0.16079853548539091 2207 21 0.19657052852556944 2207 22 0.1845949675920977 2207 23 0.5261182178383592 2207 24 0.10478029279544643 2207 25 0.14673263473737563 2208 1 0.5291798528666654 2208 2 0.8254288505810514 2208 3 0.6114455094806956 2208 4 0.5736113564115826 2208 5 0.6839551024843118 2208 6 0.7365789692272955 2208 7 0.814724965737966 2208 8 0.8205990147678861 2208 9 1.0204174782059923 2208 10 0.8672184044062234 2208 11 0.7241786591404917 2208 12 0.9236702603049949 2208 13 0.6330524554576016 2208 14 1.0083251999205487 2208 15 0.9909578963886787 2208 16 1.0167173287016027 2208 17 0.8130029093461602 2208 18 0.8779608055102983 2208 19 0.362242688374927 2208 20 0.607984257007395 2208 21 0.7840306757202313 2208 22 0.804063994140769 2208 23 1.2211511429597834 2208 24 1.184010649459431 2208 25 1.2348423454134547 2209 1 -0.3568788238574463 2209 2 -0.23243568510233814 2209 3 0.26736079293756987 2209 4 0.3782955987113539 2209 5 0.35563026445522183 2209 6 0.3651676906995718 2209 7 0.5719195237643149 2209 8 0.7143952643866882 2209 9 0.6975959169371109 2209 10 0.39358340997640084 2209 11 0.48863467960390494 2209 12 0.6702052341967146 2209 13 0.6775236616663278 2209 14 1.0047714865701636 2209 15 0.5775073091857834 2209 16 0.7462897006029934 2209 17 1.0069930261643734 2209 18 1.022785515954233 2209 19 0.8811097321957604 2209 20 0.7890680462242727 2209 21 0.72347907219266 2209 22 1.0693135320335614 2209 23 1.045183207287593 2209 24 0.9452412549599103 2209 25 1.015328973866815 2210 1 -0.07702832753348332 2210 2 -0.2844225197992145 2210 3 0.4411348283962653 2210 4 0.7250778661053232 2210 5 0.9129913018926956 2210 6 0.9303143972918164 2210 7 1.1786441114886514 2210 8 0.9105369181240093 2210 9 1.4125513536895227 2210 10 1.4649398236261675 2210 11 0.8386485292420929 2210 12 0.597061040453859 2210 13 0.9831779348680953 2210 14 1.3133061224333034 2210 15 0.8823052583530853 2210 16 1.1989873023525774 2210 17 0.9661347830950788 2210 18 1.1283161381379117 2210 19 1.3742283629463046 2210 20 1.3762908950495856 2210 21 1.4144031236734804 2210 22 1.229137451535479 2210 23 1.5222308265076308 2210 24 1.2276027902601925 2210 25 1.376603575078704 2211 1 -1.4908661731426633 2211 2 -0.5955485070935538 2211 3 -0.059987474796322715 2211 4 0.2172109610203634 2211 5 0.609675610314909 2211 6 0.38522907888658153 2211 7 0.31027527000386357 2211 8 0.4746286073295849 2211 9 0.6551871937096507 2211 10 0.6291851668316966 2211 11 0.4122335196724194 2211 12 0.4372896344748298 2211 13 0.4562786007020918 2211 14 0.6546570196468622 2211 15 0.574490469829513 2211 16 0.3065298267709835 2211 17 0.5252924716357228 2211 18 0.47258286710484043 2211 19 0.7097637707583928 2211 20 0.6184343234207645 2211 21 0.6845789721400877 2211 22 0.7031465629481062 2211 23 0.9652835990188464 2211 24 0.7992594679192635 2211 25 0.9403268541089744 2212 1 0.8842960555438424 2212 2 -0.235012965958326 2212 3 0.335310172369752 2212 4 0.3440186816969586 2212 5 0.5840331184159879 2212 6 0.5364270434656149 2212 7 0.5370498405018651 2212 8 0.6283923957319999 2212 9 0.7744209273313913 2212 10 0.7093182647987604 2212 11 0.48401277263314313 2212 12 0.6829705805809283 2212 13 0.4960729038439027 2212 14 0.412899397177668 2212 15 0.3951214834790946 2212 16 -0.0018422508335831367 2212 17 0.5656718754297643 2212 18 0.6347974647859829 2212 19 0.2221014384775799 2212 20 0.4655846511931543 2212 21 0.6366421563804963 2212 22 0.5420947077812386 2212 23 1.1769284280053611 2212 24 0.8239671247817668 2212 25 0.5401925958856566 2213 1 -0.04894101040683552 2213 2 -0.0053550912112179105 2213 3 -0.5639516941311236 2213 4 -1.5438156113854085 2213 5 -2.3937810938920308 2213 6 -1.2416624333896813 2213 7 -0.5760693480443209 2213 8 -0.6517766070064397 2213 9 -1.2087147319622957 2213 10 -1.1393497439288112 2213 11 -0.9375589762326655 2213 12 -1.1547394924803385 2213 13 -1.2249789844424797 2213 14 -7.845063762164067 2213 15 -3.6518765535688047 2213 16 -2.4727969943002286 2213 17 -1.6624041056075711 2213 18 -6.198916509109846 2213 19 -3.682978253700778 2213 20 -5.9323715053650155 2213 21 -3.0369972102937766 2213 22 -2.851810196423525 2213 23 -5.443358585842269 2213 24 -3.3453322709565683 2213 25 -2.430814375978695 2214 1 0.7059774038813345 2214 2 0.44584315366758887 2214 3 -1.411606865346208 2214 4 -1.8706345121557852 2214 5 -0.13090013042937443 2214 6 -0.1786080435588815 2214 7 -0.16505368366490103 2214 8 -0.1709307575552046 2214 9 -5.334246067480521 2214 10 -1.0182033145228075 2214 11 -0.6864200207692548 2214 12 -0.8181986725869024 2214 13 -1.256781865483191 2214 14 -2.5613204563639407 2214 15 -1.9773261971563727 2214 16 -1.2289263031633197 2214 17 -1.5427659834791 2214 18 -0.7098097321558541 2214 19 -4.355435133687028 2214 20 -2.132271466546868 2214 21 -3.5763985996359757 2214 22 -5.589464189575306 2214 23 -5.7576311124404285 2214 24 -5.302814807616546 2214 25 -3.104544916207626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22182.0 3 7.0 4 375.0 5 2749.0 6 3314.0 7 2769.0 8 2027.0 9 1732.0 10 1453.0 11 1404.0 12 1289.0 13 1302.0 14 1297.0 15 1305.0 16 1401.0 17 1411.0 18 1681.0 19 1850.0 20 2503.0 21 3146.0 22 4054.0 23 5155.0 24 6118.0 25 7035.0 26 8005.0 27 9112.0 28 10301.0 29 12291.0 30 15150.0 31 19148.0 32 24510.0 33 31610.0 34 41059.0 35 54551.0 36 74108.0 37 109864.0 38 173119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.225341267699996 18.717809581263094 9.107444380109921 29.94940477092699 2 12.66388047764106 20.86491022644476 39.44053041606517 27.030678879849006 3 19.895032603509517 24.08145954665596 28.091426147938897 27.932081701895623 4 12.389006534640867 17.222277763135175 35.596827503052225 34.79188819917173 5 12.993134325193376 38.666638394162135 34.907102103625796 13.433125177018695 6 33.91872084466096 35.767937185639305 16.879534852603985 13.433807117095748 7 28.570756306603073 31.18296055887327 20.435549537460115 19.81073359706355 8 25.816999913468674 38.819621660763815 18.998938852716098 16.364439573051413 9 26.591395253862633 15.671726763860613 18.72676234243179 39.01011563984496 10 15.648182085293413 29.879653834386954 32.813337735626156 21.658826344693473 11 34.48737459776642 22.746924096157485 22.614575052053755 20.151126254022337 12 24.569785331814987 25.061178009836937 30.527524047197925 19.841512611150147 13 30.474100792414145 21.284640672817606 24.26985994575908 23.971398589009173 14 21.119298256249223 23.420758302745202 25.828759005943848 29.631184435061726 15 25.32057300342227 30.14612798691662 21.90829522396196 22.625003785699143 16 21.563128437477285 27.765882053642816 29.505366704625303 21.165622804254596 17 22.473215164570572 29.07663302314664 27.62483815523468 20.825313657048103 18 22.57352785290492 27.01669690260796 29.9758314379083 20.433943806578817 19 24.226799440315457 27.076708279575755 26.69343945533729 22.003052824771494 20 23.971119570912418 27.61872745609333 27.38386196996266 21.0262910030316 21 22.011520534959857 27.039640452704933 27.08521985626588 23.86361915606933 22 22.9757702680398 28.754048762316643 25.030474555071496 23.239706414572062 23 21.41804729650947 28.377451403495225 27.011888483570996 23.19261281642431 24 23.558378824473603 28.28350129091363 26.935499746360076 21.222620138252683 25 22.93821653061008 27.085027415742584 26.37953200168992 23.597224051957415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 369.0 1 369.0 2 363.0 3 357.0 4 357.0 5 357.0 6 429.0 7 501.0 8 501.0 9 501.0 10 725.0 11 949.0 12 949.0 13 949.0 14 1402.0 15 1855.0 16 1855.0 17 1855.0 18 3290.0 19 4725.0 20 4725.0 21 4725.0 22 8109.5 23 11494.0 24 11494.0 25 11494.0 26 17932.5 27 24371.0 28 24371.0 29 24371.0 30 34040.5 31 43710.0 32 43710.0 33 43710.0 34 49825.5 35 55941.0 36 55941.0 37 55941.0 38 61009.0 39 66077.0 40 66077.0 41 66077.0 42 71913.5 43 77750.0 44 77750.0 45 77750.0 46 93376.5 47 109003.0 48 109003.0 49 109003.0 50 102605.0 51 96207.0 52 96207.0 53 96207.0 54 80484.0 55 64761.0 56 64761.0 57 64761.0 58 54424.5 59 44088.0 60 44088.0 61 44088.0 62 36543.0 63 28998.0 64 28998.0 65 28998.0 66 22771.5 67 16545.0 68 16545.0 69 16545.0 70 12136.5 71 7728.0 72 7728.0 73 7728.0 74 5437.0 75 3146.0 76 3146.0 77 3146.0 78 2177.5 79 1209.0 80 1209.0 81 1209.0 82 820.5 83 432.0 84 432.0 85 432.0 86 285.0 87 138.0 88 138.0 89 138.0 90 85.0 91 32.0 92 32.0 93 32.0 94 16.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09706429714697594 2 0.0342223574964377 3 0.027559597629874606 4 0.03225381480858951 5 0.022108248648141166 6 0.409305452711819 7 0.24727924686585293 8 0.2522763167657752 9 0.25803051846871605 10 0.018928295075463328 11 0.0018171163272444795 12 0.002877100851470426 13 0.0 14 0.0825273665290201 15 0.0010599845242259462 16 0.002877100851470426 17 0.004845643539318612 18 0.003785659015092665 19 0.002271395409055599 20 0.0013628372454333596 21 1.5142636060370661E-4 22 0.0 23 0.0 24 3.0285272120741323E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 660387.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.42797119649936 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2523312574228 65.36793248729538 2 6.912106832648407 10.012597491643705 3 1.3557863775126904 2.945905700972861 4 0.507745147725325 1.470998037384486 5 0.2583357850189026 0.9355368398187066 6 0.15675309674142568 0.6811985265450035 7 0.10131425340546565 0.5136590079212132 8 0.06573729396189164 0.38089750668861594 9 0.05527689460921299 0.3603233997529209 >10 0.2769566864782154 3.6698544385515284 >50 0.027033081885433796 1.3786717879826609 >100 0.021541902374043374 3.228618358807643 >500 0.00506868291135534 2.418060312459594 >1k 0.0035903170622100323 4.3533651627403875 >5k 4.22390242612945E-4 2.2823809414353238 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 7950 1.2038395667994675 No Hit GTACATGGGAAGCAGTGGTATCAAC 6971 1.0555931597684387 No Hit GAGTACATGGGAAGCAGTGGTATCA 3378 0.511518246119321 No Hit CATGTACTCTGCGTTGATACCACTG 2833 0.42899087959030086 No Hit GCGTTGATACCACTGCTTCCCATGT 2325 0.35206628840361787 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2083 0.31542110913752086 No Hit GTATCAACGCAGAGTACATGGGAAG 1948 0.29497855045602045 No Hit CTGTAGGACGTGGAATATGGCAAGA 1921 0.2908900387197204 No Hit GTCCTACAGTGGACATTTCTAAATT 1823 0.27605025538055716 No Hit ACTCTGCGTTGATACCACTGCTTCC 1608 0.24349358785076022 No Hit GCTTCCCATGTACTCTGCGTTGATA 1326 0.20079135416051497 No Hit TATCAACGCAGAGTACATGGGAAGC 1232 0.18655727626376656 No Hit CTTTAGGACGTGAAATATGGCGAGG 1201 0.18186305908505165 No Hit GTATCAACGCAGAGTACTTTTTTTT 1199 0.18156020636384423 No Hit GTCCTACAGTGTGCATTTCTCATTT 1171 0.17732026826694045 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1160 0.17565457830029965 No Hit GGTATCAACGCAGAGTACATGGGAA 1139 0.17247462472762182 No Hit CAGTGGTATCAACGCAGAGTACATG 1077 0.16308619037019204 No Hit CTGTAGGACCTGGAATATGGCGAGA 1036 0.15687770958544006 No Hit GATATACACTGTTCTACAAATCCCG 958 0.14506645345835092 No Hit CATGGGAAGCAGTGGTATCAACGCA 881 0.13340662369186554 No Hit GGTATCAACGCAGAGTACTTTTTTT 786 0.1190211194345134 No Hit GCAGTGGTATCAACGCAGAGTACAT 776 0.11750685582847632 No Hit ATACCACTGCTTCCCATGTACTCTG 764 0.11568973950123185 No Hit CTGAAGGACCTGGAATATGGCGAGA 735 0.11129837504372438 No Hit GTGGTATCAACGCAGAGTACATGGG 729 0.11038981688010212 No Hit GTGTATATCAATGAGTTACAATGAA 717 0.10857270055285764 No Hit ACATGGGAAGCAGTGGTATCAACGC 703 0.10645273150440575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.5142636060370661E-4 0.0 0.0 0.0 0.0 11 1.5142636060370661E-4 0.0 0.0 0.0 0.0 12 1.5142636060370661E-4 0.0 0.0 1.5142636060370661E-4 0.0 13 1.5142636060370661E-4 0.0 0.0 1.5142636060370661E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATAC 335 0.0 15.371546 1 CCAACGA 85 1.7753337E-8 14.488475 19 TAGTACC 40 0.0051386943 14.306258 4 ACTGTTC 375 0.0 13.683937 8 TCTAGGA 85 2.5554255E-7 13.468821 2 CACTGTT 375 0.0 13.430531 7 TAGGAGT 50 0.001454111 13.352508 4 GTACCCA 50 0.0014557831 13.350472 6 TAGGACC 415 0.0 13.329526 4 TATACAC 450 0.0 12.504729 3 TGTAGGA 955 0.0 12.487453 2 TCCAACG 160 0.0 12.433619 18 AGGACTC 70 1.05046274E-4 12.261573 5 AGGACCT 695 0.0 12.212565 5 GTAGGAC 915 0.0 12.195499 3 CTAGTCC 55 0.002976056 12.138643 3 TAGGCTT 55 0.002977756 12.137719 5 CTATTAT 55 0.0029794574 12.136793 1 GACAGTA 55 0.003058617 12.094389 7 GCGTGCG 55 0.003058617 12.094389 9 >>END_MODULE