FastQCFastQC Report
Thu 26 May 2016
SRR1926558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636958
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC19310.30315970597747416No Hit
GTACATGGGAAGCAGTGGTATCAAC18230.2862041139290189No Hit
GTCCTACAGTGGACATTTCTAAATT13220.20754900637090673No Hit
CTGTAGGACGTGGAATATGGCAAGA9380.1472624568652878No Hit
GAGTACATGGGAAGCAGTGGTATCA9150.14365154374385752No Hit
GTCCTACAGTGTGCATTTCTCATTT8290.13014986859416164No Hit
CATGTACTCTGCGTTGATACCACTG7160.1124092954323519No Hit
GTCCTAAAGTGTGTATTTCTCATTT6890.10817039742023807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAC300.002332113722.3813861
CCAGTAG300.007306507318.3758093
GTGTTAG500.002324096616.1145971
GTGTAGC1002.8292561E-816.1145971
TGTTAGG603.548627E-416.0806792
TGTAGGA6850.015.695012
GTCCTAC7600.015.5491721
TAGGACC2750.015.3264814
CTAGAAG601.1552848E-414.7006463
CTAGGAC908.753341E-814.7006463
TGTAGAA2350.014.6632322
GTCCTAA2750.014.6496341
GTGTATA2800.014.3880331
CTGTAGG6400.014.2681331
TCCTACA8550.014.0252522
TAGACCC450.006001651313.9739565
AGGAGAT601.7779844E-413.9739555
AGTACTC652.2799971E-413.5698283
GTAGGAC7350.013.5005943
GTCCTAT904.4710578E-513.4288311
Produced by FastQC (version 0.11.5)