FastQCFastQC Report
Thu 26 May 2016
SRR1926558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636958
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC54340.8531174739935757No Hit
GTACATGGGAAGCAGTGGTATCAAC49800.781841188900995No Hit
CTGTAGGACGTGGAATATGGCAAGA28890.45356208729617964No Hit
GTCCTACAGTGGACATTTCTAAATT26960.42326181632069937No Hit
GAGTACATGGGAAGCAGTGGTATCA24290.3813438248675737No Hit
CATGTACTCTGCGTTGATACCACTG20070.3150914189004613No Hit
GTCCTACAGTGTGCATTTCTCATTT17790.27929628013150004No Hit
CTTTAGGACGTGAAATATGGCGAGG17350.2723884463339812No Hit
GTCCTAAAGTGTGTATTTCTCATTT16160.25370589583614617No Hit
ACGCAGAGTACATGGGAAGCAGTGG15950.25040897516005767No Hit
CTGTAGGACCTGGAATATGGCGAGA15700.2464840695932856No Hit
GCGTTGATACCACTGCTTCCCATGT15430.24224517158117176No Hit
GTATCAACGCAGAGTACTTTTTTTT14660.23015646243551383No Hit
GTATCAACGCAGAGTACATGGGAAG13720.21539881750445083No Hit
GCTTCCCATGTACTCTGCGTTGATA12650.19860022167866642No Hit
ACTCTGCGTTGATACCACTGCTTCC11930.18729649364636286No Hit
GATATACACTGTTCTACAAATCCCG11000.1726958449379708No Hit
CTGAAGGACCTGGAATATGGCGAGA10960.17206786004728725No Hit
TATCAACGCAGAGTACATGGGAAGC9440.14820443420131313No Hit
GGTATCAACGCAGAGTACTTTTTTT9390.1474194530879587No Hit
GGTATCAACGCAGAGTACATGGGAA8630.13548774016497164No Hit
GTGTATATCAATGAGTTACAATGAA7810.12261404990595925No Hit
CAGTGGTATCAACGCAGAGTACATG7790.1223000574606175No Hit
TATCAACGCAGAGTACTTTTTTTTT7260.11397925765906072No Hit
GTTCTACAGTGTGGTTTTTATCATT6860.10769940875222542No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6770.10628644274818747No Hit
CATGGGAAGCAGTGGTATCAACGCA6650.10440248807613689No Hit
ACCTGGAATATGGCGAGAAAACTGA6570.10314651829476983No Hit
GTCCTTCAGTGTGCATTTCTCATTT6470.10157655606806101No Hit
GATATACACTGTTCTACAATGCCGG6410.1006345787320357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGG504.4095577E-617.1578452
GTGCTAG551.0915464E-515.5968091
GATCTAC508.459593E-515.2502141
TAGGACC6150.014.56714254
TGGCGAA551.998373E-413.78438518
TCTAGGA903.4680852E-813.76864052
TGTAGAA3800.013.5456672
TCCAACG1550.013.450891518
CTAGACA500.00146193813.3428834
CCAACGA1001.03791535E-813.26746919
ACTGTTC4150.013.0521398
AGGACCT9450.013.0100675
TGTAGGA14750.012.9895542
GTAGGAC14700.012.8371753
GACGTGG6550.012.7662147
CTGTAGG14350.012.7527921
GGACCTG9150.012.7075076
TACAACT603.9766086E-412.7075074
GACAGTC604.0784094E-412.6694997
TGCCGGT751.4995736E-512.646605510
Produced by FastQC (version 0.11.5)