FastQCFastQC Report
Thu 26 May 2016
SRR1926557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924111
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC22410.24250333563825124No Hit
GTACATGGGAAGCAGTGGTATCAAC22230.2405555176813175No Hit
GAGTACATGGGAAGCAGTGGTATCA10820.11708550163346179No Hit
GTCCTACAGTGGACATTTCTAAATT10110.10940244191444534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG501.8659648E-618.7756926
GTACAAG901.5968908E-716.3290881
AGAACCG453.3025473E-416.2367945
TAGGACC1600.016.1207984
GTCTTAT500.002407015816.0321961
TGTAGGA5650.015.7595762
GATATAC2050.015.6411661
CTGTAGG5600.015.2687571
GTAGGAC5700.014.9956023
CTATAGG902.860288E-614.8446251
TCCAACG1100.014.71602818
GTCCTAA3000.014.6961781
GTATTAG550.004540411314.5747211
GTCCTAC5000.014.4289751
GTTCTGG1355.640686E-913.8549841
GATTAAA802.6029692E-413.3601631
GTATATA1202.816596E-713.3601621
CAACGCG550.001511091513.2846485
AGGACGT6100.013.1757575
ACTGTTC2150.013.15708358
Produced by FastQC (version 0.11.5)