##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926557_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 924111 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.561984436934523 27.0 2.0 31.0 2.0 31.0 2 15.963509794819021 16.0 2.0 30.0 2.0 31.0 3 19.32740330977556 20.0 10.0 30.0 2.0 31.0 4 21.26295650630714 27.0 2.0 35.0 2.0 37.0 5 22.03573272041995 28.0 2.0 35.0 2.0 37.0 6 22.778912922798234 30.0 2.0 35.0 2.0 37.0 7 22.560468385291376 30.0 2.0 35.0 2.0 37.0 8 22.655856277005686 30.0 2.0 35.0 2.0 37.0 9 25.231161624523462 31.0 17.0 37.0 2.0 39.0 10 25.828390745267615 31.0 17.0 37.0 2.0 39.0 11 23.107556343339706 29.0 7.0 37.0 2.0 39.0 12 24.146429379154668 30.0 2.0 37.0 2.0 39.0 13 20.981115904907526 27.0 2.0 35.0 2.0 39.0 14 24.214307588590547 30.0 8.0 37.0 2.0 40.0 15 25.245800558590904 31.0 8.0 38.0 2.0 40.0 16 25.603898233004475 31.0 7.0 38.0 2.0 40.0 17 24.937403623590672 31.0 2.0 38.0 2.0 40.0 18 25.001294216820273 31.0 2.0 38.0 2.0 40.0 19 25.357672400826306 31.0 2.0 38.0 2.0 40.0 20 25.582211444296195 31.0 2.0 38.0 2.0 40.0 21 23.51157057972473 30.0 2.0 37.0 2.0 40.0 22 24.40872903796189 30.0 2.0 38.0 2.0 40.0 23 24.406437105499233 31.0 2.0 38.0 2.0 40.0 24 23.515279008690513 30.0 2.0 38.0 2.0 40.0 25 23.312629110572214 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.4737714092237795 1101 2 9.961474326892397 1101 3 6.788074145450167 1101 4 8.151873751480345 1101 5 7.629674247791954 1101 6 7.504534104811331 1101 7 7.1669265300259575 1101 8 5.573606089166407 1101 9 5.695419105408163 1101 10 5.189081076849153 1101 11 7.87843909117737 1101 12 5.622037487752053 1101 13 10.128815969244016 1101 14 7.53591302995974 1101 15 6.729305826705982 1101 16 6.203593830662083 1101 17 2.203387935069774 1101 18 4.9523580886473475 1101 19 5.445731771383507 1101 20 3.3475339797216996 1101 21 0.6680522956096695 1101 22 4.503317353980606 1101 23 5.583392893410931 1101 24 6.999559272265952 1101 25 7.50167679998259 1102 1 5.7799277868591155 1102 2 10.754945738558536 1102 3 7.041223788259472 1102 4 8.440147608513708 1102 5 8.27403390034246 1102 6 7.08686233979866 1102 7 7.3406354241654626 1102 8 7.2837886198345565 1102 9 6.3338730043073035 1102 10 5.787506869718971 1102 11 8.56357428308068 1102 12 5.892938870147024 1102 13 10.182535658761957 1102 14 7.8077552430598764 1102 15 5.816650108127707 1102 16 6.279087370476184 1102 17 7.067586116658816 1102 18 7.10947404226685 1102 19 6.03256362900499 1102 20 6.233022518152691 1102 21 5.433150567635767 1102 22 6.4440172781276175 1102 23 6.757787397120751 1102 24 8.110113617326512 1102 25 7.719336041722183 1103 1 4.353154329649396 1103 2 8.34193543500854 1103 3 5.710920910807584 1103 4 7.2095647441604385 1103 5 6.728207562678417 1103 6 6.269035172662413 1103 7 6.295149762585318 1103 8 5.823894321495182 1103 9 4.984551514171699 1103 10 4.26806071055158 1103 11 5.163673803732216 1103 12 3.8758910545973038 1103 13 7.837170174573572 1103 14 6.243034793607642 1103 15 5.251558157611008 1103 16 5.135504210683486 1103 17 2.7931685286454915 1103 18 1.4154548090802734 1103 19 3.6866741201470106 1103 20 3.9487929785337847 1103 21 6.059883672143663 1103 22 2.188940563958454 1103 23 4.033506039508541 1103 24 5.358536138938028 1103 25 1.3784986270813597 1104 1 5.225075187629347 1104 2 9.892111431303022 1104 3 6.506589133601921 1104 4 7.061911863737983 1104 5 7.095649596526176 1104 6 6.76933656334246 1104 7 6.634959029872849 1104 8 6.417793380997633 1104 9 5.256706505122047 1104 10 4.410196555262413 1104 11 7.504205615518494 1104 12 4.577985402570292 1104 13 8.514175334699267 1104 14 6.404367638370818 1104 15 5.607103984063908 1104 16 5.238237982778198 1104 17 5.618332588282776 1104 18 3.2580892174265763 1104 19 4.122326416823306 1104 20 3.191434683707076 1104 21 2.470725963918394 1104 22 4.533837781805225 1104 23 4.77014531134866 1104 24 5.956776810499914 1104 25 5.232805579212588 1105 1 3.287790239875786 1105 2 9.164987656293956 1105 3 6.1329897996599385 1105 4 5.483872297603355 1105 5 1.9661033988006373 1105 6 4.560256498004456 1105 7 5.552488353510103 1105 8 2.9697275080449543 1105 9 3.73563399400755 1105 10 3.3665924052323817 1105 11 6.3456059607616595 1105 12 3.7716852945755086 1105 13 4.778770671210857 1105 14 3.504066458931728 1105 15 4.117668455867378 1105 16 4.314006720878094 1105 17 4.838199376678709 1105 18 2.443858798148618 1105 19 2.1404439525632384 1105 20 1.1651425535660174 1105 21 2.263607120954557 1105 22 3.7053388319907192 1105 23 1.1837262931348782 1105 24 4.078441398956297 1105 25 4.7997921073078516 1106 1 6.297937433545517 1106 2 10.682085301607529 1106 3 7.404342196376618 1106 4 8.650912948220906 1106 5 8.338432989240491 1106 6 7.3808789579611975 1106 7 7.258085744199008 1106 8 6.875872933466649 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22 1.4003225627193316 1107 23 -1.2308523687539505 1107 24 1.7262040101322071 1107 25 2.5690136805303005 1108 1 5.11918204921351 1108 2 10.363355408476764 1108 3 0.36248994917854205 1108 4 5.623829101697762 1108 5 7.200500513553671 1108 6 7.280848185065906 1108 7 7.280709740103845 1108 8 7.055034801115088 1108 9 5.724319372492914 1108 10 5.053140795107403 1108 11 7.144756828172852 1108 12 2.817215094982039 1108 13 3.9120189138590327 1108 14 5.163511136864223 1108 15 5.238664305201418 1108 16 4.989025359878088 1108 17 1.224824170744096 1108 18 4.10615708202992 1108 19 5.103610996815203 1108 20 5.315757501951605 1108 21 5.121640594639469 1108 22 2.245450292656237 1108 23 4.794963512436343 1108 24 5.580616903854189 1108 25 6.128916741504771 1109 1 3.6814321244836457 1109 2 -13.499002461062435 1109 3 -5.334442746310081 1109 4 2.2636523934373756 1109 5 1.4616707876113075 1109 6 2.507402002283996 1109 7 3.783487858610634 1109 8 3.699721101206901 1109 9 3.4710396389306126 1109 10 2.730304765058822 1109 11 0.7218550064497045 1109 12 1.468881631552172 1109 13 2.610317251225357 1109 14 3.542845860371923 1109 15 3.629234908589133 1109 16 3.472193470616258 1109 17 4.018497390558913 1109 18 4.189770122906253 1109 19 2.577280202222756 1109 20 1.8470453461033927 1109 21 0.720654834634118 1109 22 3.108340071157599 1109 23 3.390052182515255 1109 24 4.317903684931462 1109 25 4.668369443981437 1110 1 3.0537917873387563 1110 2 8.54436648928326 1110 3 5.843454275368536 1110 4 7.238498089921091 1110 5 7.822809725191092 1110 6 3.4926763131397003 1110 7 5.864483443246261 1110 8 6.657981658456336 1110 9 4.994945850501129 1110 10 4.564940975731432 1110 11 2.446961291414432 1110 12 4.969646805219266 1110 13 9.418977377479013 1110 14 6.933089115327007 1110 15 6.239493701254396 1110 16 5.717251268555753 1110 17 6.143795749729872 1110 18 6.217494004522955 1110 19 5.234136119240453 1110 20 5.5831310924014765 1110 21 3.444605160808912 1110 22 5.470426887137236 1110 23 6.13017304872308 1110 24 7.1034403410444575 1110 25 7.28561278872731 1111 1 5.198854063505326 1111 2 9.209382249171842 1111 3 7.293813145120634 1111 4 7.946378266717048 1111 5 7.878996227410344 1111 6 7.276470172091994 1111 7 7.352753622065002 1111 8 7.433087622103578 1111 9 6.019439360155079 1111 10 5.1806949742170545 1111 11 7.236530546960232 1111 12 6.440307139513358 1111 13 7.791349792907663 1111 14 6.251408251714565 1111 15 5.520335535834079 1111 16 5.234129888350669 1111 17 6.542827448535032 1111 18 6.547246132127288 1111 19 5.26902875281975 1111 20 4.920205542471653 1111 21 7.778846163489682 1111 22 6.9770544104825625 1111 23 6.823844525378526 1111 24 6.753218571085355 1111 25 7.269498331713017 1112 1 -2.1059042859480996 1112 2 7.644728425787719 1112 3 5.945362370790608 1112 4 -0.16465881137961347 1112 5 3.9239820304171644 1112 6 3.045715676377487 1112 7 5.517928567482937 1112 8 5.705328258309869 1112 9 4.490438655018899 1112 10 3.3100358038644515 1112 11 -0.35749804310677646 1112 12 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3.7205610829509617 2204 7 4.870673625799146 2204 8 4.941255674163774 2204 9 3.311596489842696 2204 10 3.194746248688343 2204 11 4.796140328862855 2204 12 4.967235766787393 2204 13 2.66884145915294 2204 14 4.007030667472655 2204 15 4.176073476686785 2204 16 4.106235440167758 2204 17 -1.4852136426131004 2204 18 2.66560334194342 2204 19 3.4009708580214912 2204 20 3.800849449009128 2204 21 5.092334204503036 2204 22 4.790392307770251 2204 23 3.6002898532247407 2204 24 5.1672717904235554 2204 25 5.26301842413309 2205 1 5.774392787009525 2205 2 9.955542993483018 2205 3 7.014929413621083 2205 4 7.2906401055182535 2205 5 7.485496444356219 2205 6 7.246424884164021 2205 7 7.159979482928346 2205 8 6.924140987738589 2205 9 5.641701895928271 2205 10 5.020076484023086 2205 11 7.427205763353257 2205 12 6.504521974645932 2205 13 9.388787848950614 2205 14 7.104277389505317 2205 15 5.969193500721637 2205 16 5.583048247309545 2205 17 6.413789262248301 2205 18 6.105704085424065 2205 19 4.56857648358115 2205 20 5.150831208274351 2205 21 -1.079092416044812 2205 22 3.3989184368912433 2205 23 4.657743290579035 2205 24 6.384774702113038 2205 25 6.7635672068810635 2206 1 3.9987027937411668 2206 2 8.268668098249183 2206 3 5.9207061960332155 2206 4 1.6878217864825338 2206 5 5.469831598767762 2206 6 5.791272403548373 2206 7 5.919880716707883 2206 8 5.792624627492753 2206 9 4.33339232859684 2206 10 3.6298161165917655 2206 11 3.370936309155976 2206 12 4.49080782272825 2206 13 7.798075461855753 2206 14 0.11306197279161267 2206 15 2.237727466243925 2206 16 3.468179681567328 2206 17 4.408626309767762 2206 18 -0.8362981275413581 2206 19 0.9522686540851559 2206 20 2.7752014234778244 2206 21 3.1957402526766963 2206 22 -2.335271422770564 2206 23 -2.620171641388051 2206 24 1.1390659811211492 2206 25 2.603911748340714 2207 1 1.6456854728183394 2207 2 -13.499002461062435 2207 3 -5.951788584202905 2207 4 -4.0540294338517455 2207 5 1.4729302854766928 2207 6 2.1826944517699935 2207 7 3.131642228784642 2207 8 3.1967765702820046 2207 9 0.8900330578539055 2207 10 0.7170223952547126 2207 11 -1.2413580077450774 2207 12 -1.6783834455866788 2207 13 0.6475003346216255 2207 14 -4.760267435869004 2207 15 -1.0251938323753755 2207 16 -0.07455375130627928 2207 17 1.0227843378332366 2207 18 1.0385941600192083 2207 19 0.5176251307864774 2207 20 0.16080318714033837 2207 21 -3.138382971879146 2207 22 -3.672878429836498 2207 23 -0.9988424431956382 2207 24 0.6002734890403971 2207 25 1.1508995366097068 2208 1 4.747145675147028 2208 2 6.20277268686656 2208 3 6.112030846986254 2208 4 8.006774154619336 2208 5 3.98357162924459 2208 6 6.100216879714257 2208 7 6.420762289864999 2208 8 6.4950682289955 2208 9 5.067900971577899 2208 10 4.401046063893766 2208 11 6.521869707467751 2208 12 6.209915848171672 2208 13 8.481065900569327 2208 14 3.274949208528785 2208 15 4.40404838942268 2208 16 5.008725500459324 2208 17 5.827031331534325 2208 18 1.214133385062155 2208 19 3.5219198435179173 2208 20 4.222847470400502 2208 21 6.795946098554143 2208 22 5.950961997726015 2208 23 1.9510430311315226 2208 24 3.415066388448679 2208 25 4.647979131952329 2209 1 3.0586514686481188 2209 2 4.983218374870191 2209 3 5.202715842206015 2209 4 0.39862486353420223 2209 5 5.050862249272864 2209 6 5.491182414012005 2209 7 5.937083019857212 2209 8 4.521999980482171 2209 9 4.4130478300939195 2209 10 3.3693809537268073 2209 11 -0.4974538980500114 2209 12 3.078594398375248 2209 13 2.302901807356527 2209 14 3.482437387128819 2209 15 3.905770751709369 2209 16 3.550014946653036 2209 17 1.1194412729533383 2209 18 3.1965915718199724 2209 19 3.69523024155119 2209 20 3.761442264924085 2209 21 5.947848411894146 2209 22 4.713539561244122 2209 23 0.5542090622787441 2209 24 -2.9273429992747815 2209 25 0.11008199382005301 2210 1 -2.477670705053967 2210 2 -13.499002461062435 2210 3 -7.552648405385305 2210 4 -2.624090877784159 2210 5 -1.021904091615749 2210 6 -1.519899585017594 2210 7 -1.5771056541567887 2210 8 -20.173570824258938 2210 9 -12.852645661276428 2210 10 -7.287983709751394 2210 11 -9.890056656766307 2210 12 -4.787088002678331 2210 13 -5.274085785351591 2210 14 -4.242661386004887 2210 15 -6.096203324675187 2210 16 -5.467019779901342 2210 17 -4.94908024919264 2210 18 -5.103770249764555 2210 19 -10.543754994464614 2210 20 -7.366357000342433 2210 21 -5.598737849975958 2210 22 -9.98647014278305 2210 23 -8.171397092847062 2210 24 -10.650764361997455 2210 25 -8.388566373823947 2211 1 -0.21138177598022878 2211 2 4.354795007291994 2211 3 0.31519165891045375 2211 4 1.1627325097987224 2211 5 0.5412564468612473 2211 6 -0.45182042086247876 2211 7 -20.104089781140917 2211 8 -13.149520191347547 2211 9 -9.311403949401805 2211 10 -7.508829600850451 2211 11 -9.810745826949148 2211 12 -5.138795858816568 2211 13 -7.226446645307387 2211 14 -5.03609116297897 2211 15 -5.3675454986755895 2211 16 -11.535181322999593 2211 17 -7.9490079164620795 2211 18 -6.129574951392041 2211 19 -8.703441552632185 2211 20 -6.8375524996391945 2211 21 -4.758121012523677 2211 22 -6.02149324907198 2211 23 -8.184332596162312 2211 24 -21.092034203267296 2211 25 -20.91237589763347 2212 1 -1.3815257601846334 2212 2 -13.499002461062435 2212 3 -7.708975265717454 2212 4 -18.78790040159368 2212 5 -19.46317393288559 2212 6 -20.20688371200172 2212 7 -9.954717672746998 2212 8 -9.27799911242814 2212 9 -5.387349969966195 2212 10 -5.673566856867918 2212 11 -5.664846992376205 2212 12 -3.124655539056178 2212 13 -6.395001867140111 2212 14 -3.905268001684867 2212 15 -3.9976336795886915 2212 16 -4.262247174229859 2212 17 -4.3966791539230705 2212 18 -3.311724172906846 2212 19 -3.88243374686445 2212 20 -4.083037620155899 2212 21 -1.829382317500798 2212 22 -3.2242938715254823 2212 23 -3.2672117782717933 2212 24 -2.275775115362471 2212 25 -2.6969099359679056 2213 1 -18.13036911775238 2213 2 -13.499002461062435 2213 3 -8.832138394255827 2213 4 -6.277349006086533 2213 5 -19.46317393288559 2213 6 -11.070736673199812 2213 7 -20.104089781140917 2213 8 -10.897192335491068 2213 9 -8.91760984163884 2213 10 -8.066163445453252 2213 11 -7.365751384850416 2213 12 -5.969793006622272 2213 13 -18.420117531383337 2213 14 -12.417934490481533 2213 15 -7.782467817311364 2213 16 -6.978701844149779 2213 17 -5.617441622332773 2213 18 -5.764931389509766 2213 19 -6.594224053429247 2213 20 -6.516687535097745 2213 21 -4.719977868561109 2213 22 -5.651457230425006 2213 23 -5.359220361024313 2213 24 -4.5229395810753275 2213 25 -5.177181888103178 2214 1 -18.13036911775238 2214 2 -13.499002461062435 2214 3 -11.908938087924989 2214 4 -18.78790040159368 2214 5 -19.46317393288559 2214 6 -20.20688371200172 2214 7 -16.017371031140918 2214 8 -14.437633324258938 2214 9 -13.462452208895476 2214 10 -13.26903529705298 2214 11 -13.773928184544085 2214 12 -11.789270542360871 2214 13 -18.420117531383337 2214 14 -16.03053340981441 2214 15 -22.840330308802173 2214 16 -17.48575489894896 2214 17 -22.526223106335497 2214 18 -16.99555596405027 2214 19 -14.373457375416995 2214 20 -12.933666028120209 2214 21 -10.573973465055325 2214 22 -11.496740480084638 2214 23 -11.44417239046611 2214 24 -10.423284203267297 2214 25 -10.19206339763347 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 190798.0 3 17.0 4 822.0 5 3393.0 6 4712.0 7 4485.0 8 3704.0 9 3451.0 10 3296.0 11 3069.0 12 2890.0 13 3069.0 14 4484.0 15 9349.0 16 13613.0 17 14251.0 18 14843.0 19 17555.0 20 20933.0 21 23920.0 22 24679.0 23 27195.0 24 27969.0 25 28541.0 26 23809.0 27 19604.0 28 20558.0 29 23646.0 30 22923.0 31 26461.0 32 33522.0 33 41464.0 34 50056.0 35 56395.0 36 55288.0 37 57153.0 38 42194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37269487717233 17.75583590393233 12.394609597290835 25.476859621604504 2 13.787356468595458 19.73000348986662 41.19200224713363 25.290637794404297 3 19.784954878412822 24.528232565615742 27.8906519093435 27.79616064662794 4 12.529766689441743 16.590470640247577 36.42902716932599 34.450735500984685 5 12.945372765882034 38.149206194689164 35.01779057417815 13.887630465250659 6 33.40265715939909 35.414063849021346 17.43748158982641 13.745797401753157 7 28.282509576140026 31.475233502447068 21.063205512032173 19.179051409380733 8 26.464719753310355 37.024510979912584 19.391467674645853 17.119301592131208 9 26.708756816719355 15.895278724469126 19.189620170339445 38.206344288472074 10 15.73602109194771 29.45874986268263 32.66604416126552 22.13918488410414 11 33.650177887718286 22.460822211044427 23.7287746303863 20.16022527085099 12 25.00218518399249 24.589875467514368 30.06560152364829 20.342337824844854 13 29.053767296903683 21.035047232301913 24.466629085500593 25.444556385293808 14 21.04779078069974 22.649874964381628 26.380480141175276 29.921854113743358 15 24.142492276463567 30.237784544004644 22.482238654487457 23.137484525044332 16 21.88884845589368 27.66052209167078 28.07565484708514 22.3749746053504 17 22.22275492364778 28.1037043313829 28.163131511345796 21.51040923362352 18 22.178130317593624 26.661287698578455 30.122148011027615 21.038433972800302 19 23.727612506015042 26.770400058008082 26.60197886660119 22.90000856937569 20 24.05990399388894 27.243361602763866 27.07680488884645 21.61992951450074 21 22.296726468876198 25.999578719443008 26.548149423230676 25.15554538845012 22 22.906125305649034 27.857364518521617 26.498643149390023 22.73786702643933 23 21.91654389461825 27.62587745905191 27.602478551000953 22.855100095328883 24 23.24055512263023 27.401119525064395 27.144140339188734 22.214185013116648 25 22.885096222596818 26.71677492882431 27.05293651171961 23.345192336859256 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1113.0 1 1113.0 2 2196.5 3 3280.0 4 3280.0 5 3280.0 6 4568.0 7 5856.0 8 5856.0 9 5856.0 10 6671.5 11 7487.0 12 7487.0 13 7487.0 14 8618.5 15 9750.0 16 9750.0 17 9750.0 18 13347.0 19 16944.0 20 16944.0 21 16944.0 22 23875.0 23 30806.0 24 30806.0 25 30806.0 26 40860.0 27 50914.0 28 50914.0 29 50914.0 30 62550.0 31 74186.0 32 74186.0 33 74186.0 34 82173.0 35 90160.0 36 90160.0 37 90160.0 38 96265.5 39 102371.0 40 102371.0 41 102371.0 42 106603.0 43 110835.0 44 110835.0 45 110835.0 46 116039.5 47 121244.0 48 121244.0 49 121244.0 50 112094.0 51 102944.0 52 102944.0 53 102944.0 54 89584.0 55 76224.0 56 76224.0 57 76224.0 58 64663.0 59 53102.0 60 53102.0 61 53102.0 62 43392.5 63 33683.0 64 33683.0 65 33683.0 66 26203.0 67 18723.0 68 18723.0 69 18723.0 70 13743.0 71 8763.0 72 8763.0 73 8763.0 74 6199.0 75 3635.0 76 3635.0 77 3635.0 78 2531.0 79 1427.0 80 1427.0 81 1427.0 82 938.5 83 450.0 84 450.0 85 450.0 86 309.5 87 169.0 88 169.0 89 169.0 90 102.0 91 35.0 92 35.0 93 35.0 94 20.5 95 6.0 96 6.0 97 6.0 98 5.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.211940989772874 2 36.43458415709801 3 5.749417548324823 4 13.842384735167096 5 13.2932082834205 6 14.771277476407056 7 9.457305453565642 8 7.531129918375607 9 0.10961886613188243 10 1.4945174335117752 11 5.771709242720842 12 5.910437166098012 13 16.112782988190812 14 1.6415776892602727 15 2.9759412018686064 16 3.05731670762495 17 2.9449925387751037 18 1.5577133050034033 19 1.5033908264266955 20 0.2708549081225091 21 4.446327335136147 22 1.6652761410696333 23 0.10723819974007452 24 9.043935198260815 25 0.11286522939343868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 924111.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.68551321258118 #Duplication Level Percentage of deduplicated Percentage of total 1 94.90997041230618 83.22229464593963 2 3.6651160410483627 6.42755162085979 3 0.7049612848754648 1.8544467617791733 4 0.24784246666969192 0.8692877554321594 5 0.129221010918636 0.5665405330124577 6 0.07167077229141766 0.37706930704290004 7 0.04947334661593938 0.3036667051853793 8 0.03684607979248543 0.2584693933180642 9 0.027363378135078834 0.21594346695038794 >10 0.13678090492834918 2.165825235791517 >50 0.009211563377876384 0.5799190593724342 >100 0.009727027645163231 1.6769530989782848 >500 0.0012969367109779994 0.7363535831635707 >1k 5.187746843911998E-4 0.7456788331742908 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 2241 0.24250333563825124 No Hit GTACATGGGAAGCAGTGGTATCAAC 2223 0.2405555176813175 No Hit GAGTACATGGGAAGCAGTGGTATCA 1082 0.11708550163346179 No Hit GTCCTACAGTGGACATTTCTAAATT 1011 0.10940244191444534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGG 50 1.8659648E-6 18.775692 6 GTACAAG 90 1.5968908E-7 16.329088 1 AGAACCG 45 3.3025473E-4 16.236794 5 TAGGACC 160 0.0 16.120798 4 GTCTTAT 50 0.0024070158 16.032196 1 TGTAGGA 565 0.0 15.759576 2 GATATAC 205 0.0 15.641166 1 CTGTAGG 560 0.0 15.268757 1 GTAGGAC 570 0.0 14.995602 3 CTATAGG 90 2.860288E-6 14.844625 1 TCCAACG 110 0.0 14.716028 18 GTCCTAA 300 0.0 14.696178 1 GTATTAG 55 0.0045404113 14.574721 1 GTCCTAC 500 0.0 14.428975 1 GTTCTGG 135 5.640686E-9 13.854984 1 GATTAAA 80 2.6029692E-4 13.360163 1 GTATATA 120 2.816596E-7 13.360162 1 CAACGCG 55 0.0015110915 13.284648 5 AGGACGT 610 0.0 13.175757 5 ACTGTTC 215 0.0 13.1570835 8 >>END_MODULE