FastQCFastQC Report
Thu 26 May 2016
SRR1926557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924111
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC65430.7080318273454164No Hit
GTACATGGGAAGCAGTGGTATCAAC57480.6220032009141759No Hit
GAGTACATGGGAAGCAGTGGTATCA24860.2690153022742939No Hit
CATGTACTCTGCGTTGATACCACTG23330.25245884964035703No Hit
CTGTAGGACGTGGAATATGGCAAGA23010.24899606216136372No Hit
GTCCTACAGTGGACATTTCTAAATT20850.22562224667815878No Hit
GCGTTGATACCACTGCTTCCCATGT18290.1979199468462122No Hit
GTATCAACGCAGAGTACTTTTTTTT17880.193483250388752No Hit
ACGCAGAGTACATGGGAAGCAGTGG17460.18893834182257327No Hit
CTTTAGGACGTGAAATATGGCGAGG16250.17584467666762976No Hit
GTATCAACGCAGAGTACATGGGAAG16190.1751954040153185No Hit
GTCCTAAAGTGTGTATTTCTCATTT14660.15863895138138168No Hit
ACTCTGCGTTGATACCACTGCTTCC13800.1493327100315871No Hit
TATCAACGCAGAGTACATGGGAAGC10910.11805941061192866No Hit
GCTTCCCATGTACTCTGCGTTGATA10860.11751835006833596No Hit
GTCCTACAGTGTGCATTTCTCATTT10500.11362271415446845No Hit
GGTATCAACGCAGAGTACTTTTTTT10470.11329807782831283No Hit
GGTATCAACGCAGAGTACATGGGAA9760.10561501810929641No Hit
CTGAAGGACCTGGAATATGGCGAGA9390.10161117008671036No Hit
TATCAACGCAGAGTACTTTTTTTTT9260.10020441267336933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCAA401.2432203E-519.0717182
TAGACTG602.4840849E-514.2991145
GTCGTCA655.290883E-513.1948741
GCCGGTT802.038245E-613.03867311
TAGGACC4200.012.7103244
TGTAGGA10700.012.6550642
CCGGTTT751.5050284E-512.64356112
CTGTAGG10650.012.5272151
TCCAACG1457.2759576E-1212.41548518
CCAACGA1001.4902616E-712.31746919
GACGTGG5650.012.2778777
GTAGGAC11200.012.2597213
CTCTAGG1251.3387762E-912.1979271
AGGACGT9700.011.7930845
CCCTTAG657.811181E-411.7287771
GATATAC4400.011.6954561
GGACGTG9700.011.5965336
TAGGACG10200.011.5888254
CCTAGAC1001.842398E-611.4424083
TCAGTAC1001.842398E-611.4424083
Produced by FastQC (version 0.11.5)