FastQCFastQC Report
Thu 26 May 2016
SRR1926556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751405
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12940.17221072524138115No Hit
CCCATGTACTCTGCGTTGATACCAC10920.1453277526766524No Hit
GTACATGGGAAGCAGTGGTATCAAC10820.14399691245067572No Hit
CTGTAGGACGTGGAATATGGCAAGA9000.11977562033790035No Hit
GTCCTAAAGTGTGTATTTCTCATTT7850.10447095773916863No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG552.3303214E-416.9938741
CCAACGT501.3129464E-515.29595819
TAAGCTG702.794237E-614.8783095
ATTAACG450.00445320914.6172873
AAACAGT507.496244E-414.5753946
TAGACTC652.2902886E-514.4205165
CCTTTAA658.300113E-414.3794321
CTAGACT601.4649819E-414.29696754
TAGGACC2550.014.29696754
TCCAACG1150.014.04923318
AGGACCC901.7593993E-713.8864225
CCTTAGC600.00693883113.6707012
CCCTACA600.00693883113.6707012
GATATAC2900.013.3523271
TTAGGAC4700.013.2955113
CTGTAGG6050.013.2419791
GGACGTG6150.013.2041896
TGTAGGA5800.013.1992962
GTCCTAC8000.013.1854241
ATAAGAC500.00894876113.1555573
Produced by FastQC (version 0.11.5)