FastQCFastQC Report
Thu 26 May 2016
SRR1926556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751405
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC29790.3964573033184501No Hit
GTACATGGGAAGCAGTGGTATCAAC28040.37316759936385835No Hit
CTGTAGGACGTGGAATATGGCAAGA26680.35506817229057563No Hit
GTCCTACAGTGGACATTTCTAAATT24960.3321777204037769No Hit
GATATACACTGTTCTACAAATCCCG18830.25059721455140704No Hit
CTTTAGGACGTGAAATATGGCGAGG18370.24447534951191435No Hit
GTCCTAAAGTGTGTATTTCTCATTT16760.22304882187368993No Hit
GTATCAACGCAGAGTACTTTTTTTT15990.21280135213366957No Hit
GAGTACATGGGAAGCAGTGGTATCA13520.17992959855204585No Hit
GTGTATATCAATGAGTTACAATGAA13270.17660249798710417No Hit
GTCCTACAGTGTGCATTTCTCATTT11960.15916849102680977No Hit
CTGAAGGACCTGGAATATGGCGAGA11260.14985260944497308No Hit
CATGTACTCTGCGTTGATACCACTG11150.14838868519639875No Hit
GGTATCAACGCAGAGTACTTTTTTT11010.1465255088800314No Hit
GTGTATATCAATGAGTTACAATGAG10850.14439616451846873No Hit
GATATACACTGTTCTACAATGCCGG10630.14146831602132007No Hit
GTTCTACAGTGTGGTTTTTATCATT10040.1336163586880577No Hit
CTGTAGGACCTGGAATATGGCGAGA9390.12496589721920934No Hit
CTGTAGAACATATTAGATGAGTGAG9020.12004178838309566No Hit
GCGTTGATACCACTGCTTCCCATGT8530.11352067127580999No Hit
ACGCAGAGTACATGGGAAGCAGTGG8520.11338758725321231No Hit
TTGTAGAACAGTGTATATCAATGAG8240.10966123462047762No Hit
GTATCAACGCAGAGTACATGGGAAG8140.10833039439450097No Hit
TATCAACGCAGAGTACTTTTTTTTT7930.10553562991994996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC7150.014.6697821
ACTGTTC6900.014.3174838
GTCATAT400.00514726114.3030381
GCTTAGG400.00514726114.3030381
ATACACT7600.014.180424
CACTGTT6900.014.0440227
TCCTATA1257.2759576E-1213.7382762
CCAACGT707.449984E-613.53582919
TCCAACG2250.013.47566918
GTCCTAT1102.588422E-913.0027611
AATCCCG4550.012.70285319
TATACAC8950.012.576813
TAGGACC3450.012.4399224
CCTAGAC853.7585196E-612.3448343
CTGTTCT8200.012.2793249
CTAGACT701.0503282E-412.2622094
CTTAGAC550.002972781712.1407883
GCCGGTT1251.4515535E-912.13456211
ACACTGT10000.012.1123386
CCAACGA1800.012.10704719
Produced by FastQC (version 0.11.5)