FastQCFastQC Report
Thu 26 May 2016
SRR1926555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847631
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG808.497118E-716.6778321
TGCTACT550.003859172814.9351152
CTAGAAC550.001043982713.951383
TCTAGAA1006.611981E-613.6905222
CTACACT1354.9112714E-1113.50534
GTATTGA700.001461545713.3422651
GTAAAGG904.7576865E-513.3422651
GTAATAA600.00815206413.3422641
GTCTTAG600.00815206413.3422641
AGGATAG550.001509025613.2867585
TCAGAAC755.4399086E-513.1541593
GTTCTAG854.39226E-412.55742551
GTCTAGA750.00245557612.452781
ATATACT953.5142002E-612.4181854
TTTGTAC809.9537196E-512.3320253
AGGACCG852.0249658E-512.28187755
TCCAACG1255.0931703E-1112.24191418
CCCGTTT752.4244577E-512.06865212
GTAGACC1004.882497E-612.0579793
AACAGGG600.003452780211.8999637
Produced by FastQC (version 0.11.5)