FastQCFastQC Report
Thu 26 May 2016
SRR1926555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847631
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13760.1623347895487541No Hit
CCCATGTACTCTGCGTTGATACCAC12590.1485316134025301No Hit
GTACATGGGAAGCAGTGGTATCAAC11390.1343745096628132No Hit
GATATACACTGTTCTACAAATCCCG11020.13000940267640046No Hit
GTGTATATCAATGAGTTACAATGAA8770.10346483316443122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTAC350.002116526216.345631
TCCAACG1450.015.023581518
CTATTAG456.572209E-414.8321461
GATATAC4100.013.2559071
CCAACGA1251.07320375E-1012.88108819
ACTGTTC4650.012.6748788
TAGGACC1001.3668432E-712.39911654
ATACACT5100.012.3430154
TCCTATG802.7353957E-511.9278982
CTGGACT1603.6379788E-1211.9222294
GTCCTAT802.7571132E-511.9186891
TATACAC5600.011.7540063
CTAGCAC657.7425595E-411.7409023
CACTGTT4700.011.7303097
CCTAGAC1251.7176717E-811.447383
CCTAAAC855.095986E-511.222923
TTATCCC600.00570090511.1274144
CACTGCG600.00584621711.0898617
ACATTCG600.006005225211.049952518
TACACTG7250.010.9191175
Produced by FastQC (version 0.11.5)