##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 847631 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.282466073090767 31.0 28.0 31.0 22.0 34.0 2 28.753916503761662 31.0 28.0 33.0 23.0 34.0 3 29.176284255766955 31.0 28.0 33.0 25.0 34.0 4 32.978618054318446 35.0 33.0 37.0 27.0 37.0 5 32.63636653213486 35.0 33.0 37.0 26.0 37.0 6 32.775939058387436 35.0 33.0 37.0 26.0 37.0 7 32.58851906077055 35.0 33.0 37.0 25.0 37.0 8 32.58190651356546 35.0 33.0 37.0 25.0 37.0 9 33.797450777519934 37.0 34.0 39.0 23.0 39.0 10 33.71116205046771 37.0 33.0 39.0 23.0 39.0 11 33.96279159209609 37.0 34.0 39.0 25.0 39.0 12 33.74366322137817 37.0 33.0 39.0 25.0 39.0 13 33.88702985143299 37.0 34.0 39.0 25.0 39.0 14 34.54516293056766 38.0 34.0 40.0 23.0 41.0 15 34.66008439993346 38.0 34.0 40.0 24.0 41.0 16 34.61932727802546 38.0 34.0 40.0 24.0 41.0 17 34.53274833034658 38.0 34.0 40.0 23.0 41.0 18 34.49604721866001 38.0 34.0 40.0 23.0 41.0 19 34.516536087047314 39.0 34.0 40.0 22.0 41.0 20 34.496020084211175 39.0 34.0 40.0 22.0 41.0 21 34.41029056275667 39.0 34.0 40.0 19.0 41.0 22 34.337387377290355 39.0 34.0 40.0 19.0 41.0 23 33.98473746240994 39.0 34.0 40.0 17.0 41.0 24 34.04997339644255 39.0 34.0 40.0 18.0 41.0 25 33.84234295347858 38.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6325586610301706 1101 2 0.7548673761895124 1101 3 0.7529390085802667 1101 4 0.5917734781810466 1101 5 0.6191448057823905 1101 6 0.3234153719161128 1101 7 0.49274068788154324 1101 8 0.47704950699402815 1101 9 0.8279414889306338 1101 10 0.9765032971708578 1101 11 0.6876284117211853 1101 12 0.6703526624206617 1101 13 0.6294159542129734 1101 14 0.9766563477274914 1101 15 0.927152828371689 1101 16 0.8402306489596327 1101 17 0.6982356028131989 1101 18 0.7742976072247814 1101 19 0.6576381413830177 1101 20 0.8083856733125074 1101 21 0.646903025415746 1101 22 0.7480457753212448 1101 23 1.0531806003482203 1101 24 0.6650157420679648 1101 25 0.9155737135534352 1102 1 0.37142046302842857 1102 2 0.32356315954924497 1102 3 0.4233704101725628 1102 4 0.20780068850561406 1102 5 0.40850205786877325 1102 6 0.12639698711768688 1102 7 0.32601984743361356 1102 8 0.5698704523608029 1102 9 0.7149432101482418 1102 10 0.9447478609347755 1102 11 0.48957318189406607 1102 12 0.5610464296928441 1102 13 0.6534032816626265 1102 14 0.9542818903224841 1102 15 0.8456014009954202 1102 16 0.667515033881493 1102 17 0.5574235446895273 1102 18 0.7395344975125866 1102 19 0.7165655005140366 1102 20 0.7584415614539353 1102 21 0.9289523029843707 1102 22 0.9303919800707092 1102 23 0.9959607439430229 1102 24 0.8837694708013331 1102 25 0.8371122489412599 1103 1 0.48769263381120354 1103 2 0.44535912844596837 1103 3 0.5632267600444898 1103 4 0.3749936349306182 1103 5 0.6968306275858609 1103 6 0.23011118155309873 1103 7 0.47733169600196845 1103 8 0.43551935958152654 1103 9 0.7552028985449866 1103 10 0.8716819747024758 1103 11 0.7243980690790792 1103 12 0.8825106569316006 1103 13 0.6569603052867379 1103 14 1.1362862602985828 1103 15 0.8983748525348929 1103 16 0.889111615487657 1103 17 1.001823300518538 1103 18 1.0491724056289868 1103 19 1.022344023735954 1103 20 0.9222186812631676 1103 21 0.918211346724064 1103 22 0.7866177697755958 1103 23 1.106329738791473 1103 24 1.067897500832082 1103 25 1.13985574960018 1104 1 0.7363776111578453 1104 2 0.817706053793291 1104 3 0.6097215995910439 1104 4 0.351726475717939 1104 5 0.49959132918193916 1104 6 0.3158113676678127 1104 7 0.5536970072271288 1104 8 0.7838377473393194 1104 9 0.9610081736751681 1104 10 0.9496508803855548 1104 11 0.856066709689685 1104 12 0.6108470921768401 1104 13 0.5936963419832963 1104 14 0.9846422469885567 1104 15 0.8080603374671185 1104 16 0.8631081939848286 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2203 1 0.2945157850126101 2203 2 0.4122982247595992 2203 3 0.1751356586250168 2203 4 -0.05686054133904861 2203 5 0.2171032936143007 2203 6 0.13787217624633996 2203 7 0.526640871148814 2203 8 0.5533909808038615 2203 9 0.9386349211430556 2203 10 0.8871413229987581 2203 11 0.6047447225083857 2203 12 0.673839002615864 2203 13 0.42829077840425356 2203 14 1.006524651013514 2203 15 0.8595314670794423 2203 16 0.7657559441051802 2203 17 0.976235006129059 2203 18 0.9876718560420699 2203 19 1.0839391259118472 2203 20 1.018490945443098 2203 21 1.110445624401379 2203 22 1.100984444715614 2203 23 1.2629235999646369 2203 24 1.1769195906806758 2203 25 0.8262543596771224 2204 1 0.09526333332388504 2204 2 0.12188919252639607 2204 3 -0.054615993495282567 2204 4 -0.16415106404485869 2204 5 -0.06601039923582164 2204 6 -0.1514820036661888 2204 7 0.051559469303064986 2204 8 0.24583554084856019 2204 9 0.2818135428026878 2204 10 0.27927761569860365 2204 11 0.08361106934768259 2204 12 0.07467903113059293 2204 13 -0.12256273680681318 2204 14 0.19070090286793828 2204 15 0.041700127850496926 2204 16 0.1358212582488747 2204 17 0.19227646808693777 2204 18 0.1412410138942164 2204 19 0.34165640622319415 2204 20 0.5132263171942526 2204 21 0.28996607601304447 2204 22 0.2625377267995006 2204 23 0.5636759649531697 2204 24 0.19587225237653172 2204 25 0.13507465908178062 2205 1 0.5794193583993419 2205 2 0.45842042075950573 2205 3 0.5060155365720505 2205 4 0.30793623569974216 2205 5 0.39288674494806486 2205 6 0.16657464829572888 2205 7 0.23941876811894502 2205 8 0.303334960398125 2205 9 0.8702881190738765 2205 10 0.7717712307438802 2205 11 0.6164784944695754 2205 12 0.6692181534895454 2205 13 0.6032765945142913 2205 14 1.1483187343051355 2205 15 1.0070820642331313 2205 16 0.8502814393494091 2205 17 1.00692703692836 2205 18 0.9244636746789823 2205 19 0.8465646950553278 2205 20 0.9189124096019583 2205 21 0.8574445993471471 2205 22 0.7057836056014466 2205 23 1.0524546972494306 2205 24 0.8989277434375964 2205 25 0.9970574777490668 2206 1 0.35252286229960816 2206 2 0.6451712762826709 2206 3 0.6226215580724705 2206 4 0.48332758713828383 2206 5 0.7463896082141446 2206 6 0.3804676531696174 2206 7 0.650109984747786 2206 8 0.7854651842744289 2206 9 0.9317115713546897 2206 10 0.8734633806707137 2206 11 0.8810175489580487 2206 12 0.6436070737444197 2206 13 0.5126397626577202 2206 14 0.7896290386063711 2206 15 0.9025803416646028 2206 16 0.738500453279741 2206 17 0.8075814977471296 2206 18 0.8389426985184087 2206 19 0.7595642687340245 2206 20 0.6750326552552082 2206 21 0.68794201261381 2206 22 0.7531638035528019 2206 23 0.9631065079627703 2206 24 0.8692497888580988 2206 25 1.0676226415371062 2207 1 -1.2967411396982023 2207 2 -0.30151962305463087 2207 3 0.002042286254972936 2207 4 0.016270145534861058 2207 5 0.12826320061507346 2207 6 0.15878265014053738 2207 7 0.24547895490552918 2207 8 0.2366798237672043 2207 9 0.5389563999455476 2207 10 0.0928504420894356 2207 11 0.021016716538063918 2207 12 0.23429136921651406 2207 13 -0.16060672152398325 2207 14 -5.671436828937114 2207 15 -2.380776808129376 2207 16 -0.9885194369333092 2207 17 -0.8976965074046248 2207 18 -0.3384626483926212 2207 19 -0.14032104229045217 2207 20 0.16963764885019827 2207 21 0.2607283535267868 2207 22 0.016409686542303348 2207 23 0.301747906058381 2207 24 -0.09686840553046494 2207 25 -0.15634272979157515 2208 1 0.3785640508711765 2208 2 0.7726393556053281 2208 3 0.5062450632561841 2208 4 0.3304347154724212 2208 5 0.5455029732364522 2208 6 0.48377566104353065 2208 7 0.6084087843715622 2208 8 0.660414797208638 2208 9 0.8578083242769239 2208 10 0.8917694617520908 2208 11 0.5793057855903427 2208 12 0.5842680722264859 2208 13 0.5567914706295838 2208 14 0.8356454560316706 2208 15 0.748092821914426 2208 16 0.66126157136042 2208 17 0.6862751634554982 2208 18 0.8824673674493368 2208 19 0.4911671694325932 2208 20 0.42369598451323043 2208 21 0.6920208705335895 2208 22 0.8723876941837148 2208 23 1.0467483719981843 2208 24 1.0779988016263715 2208 25 1.0384014692579058 2209 1 -0.20939420092269145 2209 2 -0.12886187029835483 2209 3 0.39897871599928436 2209 4 0.5060426062432839 2209 5 0.6211742960877444 2209 6 0.6684565886814724 2209 7 0.7535859218302292 2209 8 0.9254623712689636 2209 9 1.0404342324578622 2209 10 0.6544830951506597 2209 11 0.751896378747773 2209 12 0.852865143553835 2209 13 0.6829025442512915 2209 14 0.959212948215125 2209 15 0.9851767457059566 2209 16 0.8998080319852946 2209 17 1.0621111397921794 2209 18 0.8075612784194846 2209 19 1.0026956126473792 2209 20 0.7670713809637348 2209 21 0.5761588015680701 2209 22 0.9272330503705888 2209 23 1.0416306177637509 2209 24 1.03609898832363 2209 25 1.134649529180976 2210 1 -0.09731872922457896 2210 2 -0.2475085846770142 2210 3 0.5488681109244631 2210 4 0.7060686309667688 2210 5 0.910699344884101 2210 6 1.0076107851681328 2210 7 1.0947049849401793 2210 8 1.1316432430972014 2210 9 1.491624863557405 2210 10 1.5963698876034869 2210 11 1.2656374327490738 2210 12 1.1135417863645074 2210 13 1.240065849391172 2210 14 1.3806052709216985 2210 15 0.9968358218282702 2210 16 1.2849088777888085 2210 17 1.3156830330908136 2210 18 1.3010290728241714 2210 19 1.4015375124521938 2210 20 1.5475788630717346 2210 21 1.7131529460052874 2210 22 1.5643573184252446 2210 23 1.804024892324584 2210 24 1.5852596909911583 2210 25 1.7155864192265184 2211 1 -1.5971171191246079 2211 2 -0.5231860641336397 2211 3 0.034062699431295584 2211 4 0.339270060312856 2211 5 0.48948217604607436 2211 6 0.303111125819683 2211 7 0.4540392544859344 2211 8 0.41888396555656726 2211 9 0.6797501677105728 2211 10 0.7119923066466072 2211 11 0.232572820188075 2211 12 0.3656956774394331 2211 13 0.37214910741038665 2211 14 0.6927844675402071 2211 15 0.22683151154337367 2211 16 0.297827496829143 2211 17 0.233161377385251 2211 18 0.27992424947633054 2211 19 0.5445455351437616 2211 20 0.3293471922106548 2211 21 0.7627651594061007 2211 22 0.7050761627127216 2211 23 0.8632726780895226 2211 24 0.6481097625975067 2211 25 0.8037192316178974 2212 1 1.1763405661024002 2212 2 0.42631301537708666 2212 3 0.6590973663515278 2212 4 0.6427129807334566 2212 5 0.8904166641510258 2212 6 0.7721174464246445 2212 7 0.9444622041191906 2212 8 0.7236010438681504 2212 9 1.1176558367094955 2212 10 0.7594293317097964 2212 11 0.536004647194197 2212 12 0.8545386078969344 2212 13 0.664541402241646 2212 14 0.7772428579914248 2212 15 0.7551532628237396 2212 16 0.1638322564322081 2212 17 0.7891881389270239 2212 18 0.9481001487692566 2212 19 0.8956309729242307 2212 20 0.9612476261744618 2212 21 1.1945298311070687 2212 22 1.0182749487762237 2212 23 1.4348032787450862 2212 24 1.3017052174671235 2212 25 0.9734210036587925 2213 1 0.35517321918642963 2213 2 0.13893827483562404 2213 3 -0.5638847236760469 2213 4 -1.1051327102294266 2213 5 -1.9967234113329013 2213 6 -0.7916815573663172 2213 7 -0.4744663264026343 2213 8 -0.5585116121212579 2213 9 -1.064250369901913 2213 10 -0.5881045899623558 2213 11 -0.5662389183737346 2213 12 -0.9093844331387189 2213 13 -1.016899105975817 2213 14 -7.699975914095727 2213 15 -3.5998217760775795 2213 16 -2.2912866544984354 2213 17 -1.8258008122598497 2213 18 -6.027574583111946 2213 19 -3.976448634402903 2213 20 -5.430940821041265 2213 21 -2.6392340008615776 2213 22 -2.258814198802952 2213 23 -5.218548380811786 2213 24 -2.9253131115500537 2213 25 -2.232509876307237 2214 1 0.18157626321233167 2214 2 0.0630717831280947 2214 3 -1.5714184904607755 2214 4 -2.10003916299868 2214 5 -0.5369051452042655 2214 6 -0.6421559531488796 2214 7 -0.776031421396894 2214 8 -0.8603924228529642 2214 9 -6.136751858041194 2214 10 -2.349567537760734 2214 11 -1.302568920248298 2214 12 -1.88677984407893 2214 13 -1.82009178137071 2214 14 -3.2249802716306455 2214 15 -2.7801309801779404 2214 16 -1.9220077689749857 2214 17 -2.237970338456037 2214 18 -1.5026235026077188 2214 19 -4.544893504186607 2214 20 -2.5276412627144254 2214 21 -4.129920057736452 2214 22 -6.252883347126865 2214 23 -6.57898919891019 2214 24 -6.235738637167426 2214 25 -4.325222433571824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26006.0 3 8.0 4 481.0 5 3146.0 6 3968.0 7 3217.0 8 2358.0 9 2026.0 10 1843.0 11 1748.0 12 1633.0 13 1570.0 14 1556.0 15 1651.0 16 1751.0 17 1828.0 18 1972.0 19 2424.0 20 3178.0 21 4085.0 22 5167.0 23 6459.0 24 7907.0 25 9008.0 26 10622.0 27 11892.0 28 13917.0 29 16346.0 30 20405.0 31 25482.0 32 32999.0 33 41939.0 34 54736.0 35 72055.0 36 96945.0 37 140684.0 38 214619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.497558833010004 18.309519901857414 9.301702263820784 29.891219001311796 2 14.608424138150768 20.269948148620166 36.91100773876534 28.210619974463725 3 19.341234056261676 22.459592675763158 28.49417004058138 29.70500322739379 4 13.303334242039089 16.00112821130123 34.63219249456252 36.06334505209716 5 14.672044292251451 37.138184366968666 33.54085492918856 14.648916411591317 6 34.76829975959546 35.47139422792245 15.956229793583685 13.804076218898404 7 29.906095625914865 30.975555539790527 19.315545655119575 19.802803179175033 8 27.175563312333278 36.16975991827005 18.224643850388787 18.43003291900789 9 26.288056967645883 15.866061704701462 18.2296230339623 39.61625829369035 10 15.855789527089609 29.14626937521459 31.760544352531273 23.237396745164528 11 36.66542396556932 22.31694540924192 21.225295416792513 19.792335208396256 12 23.478694825810425 24.223140096105407 29.773799537759654 22.524365540324514 13 29.58905467119537 20.503379418638534 24.71275826391437 25.194807646251732 14 22.360392159177433 20.507965364388124 25.637907933491128 31.49373454294332 15 24.730982361220406 28.105839756211797 22.41874611412412 24.74443176844368 16 24.330357142857142 27.441553722440347 24.632144744790093 23.59594438991241 17 23.35598927333068 27.070210604678817 25.80547352723609 23.768326594754406 18 23.366115628377134 27.353435298226074 26.316994096355405 22.96345497704138 19 24.49606074405808 26.333082435330844 25.652590423561445 23.51826639704963 20 24.088331827158804 26.74069337511296 26.09724617813129 23.07372861959694 21 24.65553996963301 25.689952349548328 26.029132959979044 23.625374720839613 22 24.09503663740472 25.76286143380787 25.867741977346277 24.274359951441134 23 24.412745640496865 25.583656095635952 25.497179786959183 24.506418476907992 24 23.966117291742858 26.308648820829845 25.75097625143046 23.97425763599684 25 24.54832886990786 25.60574779089933 25.726555218668523 24.119368120524285 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 165.0 1 165.0 2 163.5 3 162.0 4 162.0 5 162.0 6 241.0 7 320.0 8 320.0 9 320.0 10 533.5 11 747.0 12 747.0 13 747.0 14 1312.5 15 1878.0 16 1878.0 17 1878.0 18 3550.5 19 5223.0 20 5223.0 21 5223.0 22 8798.0 23 12373.0 24 12373.0 25 12373.0 26 18643.5 27 24914.0 28 24914.0 29 24914.0 30 34475.0 31 44036.0 32 44036.0 33 44036.0 34 51654.0 35 59272.0 36 59272.0 37 59272.0 38 68058.0 39 76844.0 40 76844.0 41 76844.0 42 85700.5 43 94557.0 44 94557.0 45 94557.0 46 103618.0 47 112679.0 48 112679.0 49 112679.0 50 114216.5 51 115754.0 52 115754.0 53 115754.0 54 109355.5 55 102957.0 56 102957.0 57 102957.0 58 91884.5 59 80812.0 60 80812.0 61 80812.0 62 68453.5 63 56095.0 64 56095.0 65 56095.0 66 44529.0 67 32963.0 68 32963.0 69 32963.0 70 24284.5 71 15606.0 72 15606.0 73 15606.0 74 11019.5 75 6433.0 76 6433.0 77 6433.0 78 4553.0 79 2673.0 80 2673.0 81 2673.0 82 1757.0 83 841.0 84 841.0 85 841.0 86 547.0 87 253.0 88 253.0 89 253.0 90 160.5 91 68.0 92 68.0 93 68.0 94 37.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08258310514834874 2 0.024656955680006983 3 0.02335922117053293 4 0.03208943514335837 5 0.01993792110010134 6 0.3800002595469019 7 0.2214406976620723 8 0.2263956839709732 9 0.2309967426863812 10 0.010853779533782979 11 0.0017696379674646161 12 0.0023595172899528215 13 0.0 14 0.06205530472575921 15 0.0011797586449764108 16 0.0017696379674646161 17 0.002831420747943386 18 0.0022415414254551803 19 0.0010617827804787696 20 0.0010617827804787696 21 0.0 22 0.0 23 0.0 24 1.1797586449764107E-4 25 1.1797586449764107E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 847631.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.41161219714756 #Duplication Level Percentage of deduplicated Percentage of total 1 87.89739774426666 68.9217666506187 2 8.46045646313072 13.267960623957133 3 1.8919143440149826 4.450441615593709 4 0.6523911840159776 2.046201780875951 5 0.33204007159492543 1.3017898663907201 6 0.18668490614036237 0.8782958678003392 7 0.12824833919520676 0.7039311326531954 8 0.08601072246763099 0.5395391531942694 9 0.05822009000280777 0.41086180074448714 >10 0.2788061285585378 3.7223238854005727 >50 0.015882595358797942 0.8752898748724012 >100 0.009830148669684381 1.508154777158859 >500 0.0015123304169321092 0.7952260889120865 >1k 6.049321667728437E-4 0.5782168818275176 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1376 0.1623347895487541 No Hit CCCATGTACTCTGCGTTGATACCAC 1259 0.1485316134025301 No Hit GTACATGGGAAGCAGTGGTATCAAC 1139 0.1343745096628132 No Hit GATATACACTGTTCTACAAATCCCG 1102 0.13000940267640046 No Hit GTGTATATCAATGAGTTACAATGAA 877 0.10346483316443122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1797586449764107E-4 0.0 10 0.0 0.0 0.0 1.1797586449764107E-4 0.0 11 0.0 0.0 0.0 1.1797586449764107E-4 0.0 12 0.0 0.0 0.0 1.1797586449764107E-4 2.3595172899528214E-4 13 0.0 0.0 0.0 1.1797586449764107E-4 2.3595172899528214E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATTAC 35 0.0021165262 16.34563 1 TCCAACG 145 0.0 15.0235815 18 CTATTAG 45 6.572209E-4 14.832146 1 GATATAC 410 0.0 13.255907 1 CCAACGA 125 1.07320375E-10 12.881088 19 ACTGTTC 465 0.0 12.674878 8 TAGGACC 100 1.3668432E-7 12.3991165 4 ATACACT 510 0.0 12.343015 4 TCCTATG 80 2.7353957E-5 11.927898 2 CTGGACT 160 3.6379788E-12 11.922229 4 GTCCTAT 80 2.7571132E-5 11.918689 1 TATACAC 560 0.0 11.754006 3 CTAGCAC 65 7.7425595E-4 11.740902 3 CACTGTT 470 0.0 11.730309 7 CCTAGAC 125 1.7176717E-8 11.44738 3 CCTAAAC 85 5.095986E-5 11.22292 3 TTATCCC 60 0.005700905 11.127414 4 CACTGCG 60 0.005846217 11.089861 7 ACATTCG 60 0.0060052252 11.0499525 18 TACACTG 725 0.0 10.919117 5 >>END_MODULE