FastQCFastQC Report
Thu 26 May 2016
SRR1926553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614238
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38820.6320025788049582No Hit
CCCATGTACTCTGCGTTGATACCAC36370.5921157596892409No Hit
GAGTACATGGGAAGCAGTGGTATCA19570.3186061428957505No Hit
CATGTACTCTGCGTTGATACCACTG13460.2191333001214513No Hit
GCGTTGATACCACTGCTTCCCATGT13390.21799367671814507No Hit
GTCCTACAGTGGACATTTCTAAATT12360.20122493235521086No Hit
GTATCAACGCAGAGTACATGGGAAG11360.18494459802226498No Hit
ACGCAGAGTACATGGGAAGCAGTGG10960.17843246428908663No Hit
CTGTAGGACGTGGAATATGGCAAGA9540.1553143895363035No Hit
ACTCTGCGTTGATACCACTGCTTCC9190.14961627251977247No Hit
GCTTCCCATGTACTCTGCGTTGATA8680.14131330200997008No Hit
GNACATGGGAAGCAGTGGTATCAAC8550.13919685854668712No Hit
CNCATGTACTCTGCGTTGATACCAC8420.13708041508340416No Hit
GTCCTAAAGTGTGTATTTCTCATTT7500.12210250749709395No Hit
GGTATCAACGCAGAGTACATGGGAA7370.11998606403381101No Hit
TATCAACGCAGAGTACATGGGAAGC7030.11445075036060941No Hit
CTTTAGGACGTGAAATATGGCGAGG6770.1102178634340435No Hit
GTCCTACAGTGTGCATTTCTCATTT6770.1102178634340435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA350.005792239719.1288591
CTTATAC358.340088E-418.779343
GGACGGG401.3748372E-418.2080826
TTCAACG300.00855194717.8808774
AGGACGG350.001175248417.844475
ATTAGCC452.2820501E-417.0405123
GTGTAGG654.349823E-516.4802471
TAGGACC2500.015.8782194
GTTGTGA708.196445E-515.3030871
GTACTGG708.196445E-515.3030871
TGTAGGA7400.015.207642
CTAAGAC651.4192508E-515.1679293
TGTAGAA2850.014.4462812
GATTAGA458.6995837E-414.29128311
CTGTAGG7100.014.1445771
GATATAC2500.013.9258091
GTAGGAC7500.013.8758453
CGGGGAA400.00657804213.7822419
CCTAGCA802.069715E-413.7239682
TCTAGGA802.069715E-413.7239682
Produced by FastQC (version 0.11.5)