##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614238 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.92865957495303 27.0 7.0 30.0 2.0 31.0 2 16.298486905727096 16.0 2.0 30.0 2.0 31.0 3 19.872925478397626 23.0 15.0 31.0 2.0 31.0 4 22.08652183681244 28.0 2.0 35.0 2.0 37.0 5 22.443136048241886 28.0 2.0 35.0 2.0 37.0 6 23.204139112200806 30.0 2.0 35.0 2.0 37.0 7 23.207701249352855 30.0 2.0 35.0 2.0 37.0 8 23.3472627873886 30.0 7.0 35.0 2.0 37.0 9 26.00578765885536 31.0 17.0 37.0 2.0 39.0 10 26.70193149886526 31.0 23.0 37.0 2.0 39.0 11 23.81244403635073 30.0 7.0 37.0 2.0 39.0 12 24.905800357516142 31.0 10.0 37.0 2.0 39.0 13 21.596288409378776 27.0 2.0 35.0 2.0 39.0 14 24.933029216687995 30.0 16.0 38.0 2.0 40.0 15 26.01477114734028 31.0 17.0 38.0 2.0 40.0 16 26.36072336781508 31.0 17.0 38.0 2.0 40.0 17 25.71189831954389 31.0 10.0 38.0 2.0 40.0 18 25.798205255943135 31.0 11.0 38.0 2.0 40.0 19 26.17872062620678 31.0 13.0 39.0 2.0 40.0 20 26.41683842419388 31.0 11.0 39.0 2.0 40.0 21 24.31383274886933 30.0 2.0 37.0 2.0 40.0 22 25.231814052533384 31.0 2.0 38.0 2.0 40.0 23 25.217106398497 31.0 2.0 38.0 2.0 40.0 24 24.258935461498638 31.0 2.0 38.0 2.0 40.0 25 24.065739990036434 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.337871196198176 1101 2 10.13836304395892 1101 3 6.583996714030828 1101 4 7.870346513110533 1101 5 7.63540021145905 1101 6 7.215889663144839 1101 7 7.10886502490294 1101 8 5.58115471563924 1101 9 5.4236903268861845 1101 10 4.86174932809902 1101 11 7.524487827128198 1101 12 5.097524889105834 1101 13 9.463674736551713 1101 14 6.885138911770088 1101 15 6.374358430150149 1101 16 5.780329203502934 1101 17 1.6303890472998503 1101 18 4.044633163299917 1101 19 4.67593172824218 1101 20 3.0127277976405935 1101 21 0.46754793241030157 1101 22 3.908029460055854 1101 23 5.025496238996265 1101 24 6.473110889665318 1101 25 6.879995925403112 1102 1 5.189358774679757 1102 2 10.54223822070273 1102 3 6.66586115061185 1102 4 7.930012659619248 1102 5 7.911924603936541 1102 6 6.884122298294329 1102 7 7.103399703320779 1102 8 7.093623854155105 1102 9 6.244609467966207 1102 10 5.582336593753212 1102 11 8.356398052261351 1102 12 5.456763553691868 1102 13 10.095802182576168 1102 14 7.490853689384245 1102 15 5.245027748889758 1102 16 5.796917480168354 1102 17 6.5964615617140865 1102 18 6.7407327112753315 1102 19 5.52697424199706 1102 20 5.871530555210356 1102 21 4.892771563698901 1102 22 5.684138092480012 1102 23 6.1195990397904225 1102 24 7.724403958377451 1102 25 7.192361764945371 1103 1 3.951389545265659 1103 2 8.319709336576887 1103 3 5.481663373258179 1103 4 6.950356333004748 1103 5 6.121762089439425 1103 6 5.528219953798828 1103 7 5.661505609335432 1103 8 5.253642645352603 1103 9 4.10121281221214 1103 10 3.1892372206158157 1103 11 4.345479338871154 1103 12 2.859119059659541 1103 13 7.0697147154240625 1103 14 5.145451660522141 1103 15 4.1850390529695005 1103 16 3.9949664253655897 1103 17 1.6832974470290587 1103 18 0.7434339459157258 1103 19 2.756971893097372 1103 20 3.0195589475353266 1103 21 5.205780797810796 1103 22 1.3081306347235042 1103 23 3.1989661581309257 1103 24 4.557727504628755 1103 25 0.43893016930845263 1104 1 4.805029300590807 1104 2 9.744990958487724 1104 3 6.441918170591631 1104 4 6.968330968956913 1104 5 7.135340676051719 1104 6 6.587038804752513 1104 7 6.452465509845894 1104 8 6.5516955136301895 1104 9 5.265732066619034 1104 10 4.164968569942669 1104 11 7.147888489594912 1104 12 4.299609710824349 1104 13 8.534965680675029 1104 14 6.04126239902951 1104 15 5.582245247928505 1104 16 5.034577866662424 1104 17 5.608669448243756 1104 18 3.5677004274855797 1104 19 4.240197494300503 1104 20 3.3571509536666575 1104 21 2.8456951905341796 1104 22 4.455246450378645 1104 23 4.822572509265363 1104 24 5.553689357332182 1104 25 4.629211140488298 1105 1 3.475420875155301 1105 2 9.455297727614871 1105 3 6.27931573562444 1105 4 5.427984603548548 1105 5 1.8302834757537276 1105 6 4.634067042615946 1105 7 6.02405772938074 1105 8 2.9081564429181697 1105 9 3.316270902610828 1105 10 3.030922911756548 1105 11 6.4765886498169785 1105 12 3.852416658542971 1105 13 4.900542429085565 1105 14 3.511345775735059 1105 15 3.895928416447422 1105 16 4.320773255940811 1105 17 4.991776225535084 1105 18 2.1135116920152726 1105 19 2.4541653886559907 1105 20 1.4919033238306554 1105 21 2.2091543543178673 1105 22 3.7886818359831516 1105 23 1.1871516967251807 1105 24 4.000014965308587 1105 25 4.620904085614793 1106 1 6.46175776164824 1106 2 11.130965514529654 1106 3 7.954426659755757 1106 4 9.343572195699963 1106 5 8.670324437373406 1106 6 7.815693179781931 1106 7 7.63547675924913 1106 8 7.4218146645120555 1106 9 6.207617434325915 1106 10 5.616198742281107 1106 11 8.126109160793618 1106 12 5.558530897760786 1106 13 4.93622241317054 1106 14 5.883350498061912 1106 15 6.147361339349239 1106 16 5.785860486905971 1106 17 6.599960070154879 1106 18 6.597246080697342 1106 19 6.35780855946366 1106 20 5.725538651270863 1106 21 5.892367101991354 1106 22 6.315405542357478 1106 23 6.881722651479876 1106 24 7.900232166253705 1106 25 6.963368174895471 1107 1 3.8410471727501587 1107 2 -13.79901653214898 1107 3 -5.720227265287102 1107 4 2.050147093751324 1107 5 4.491200799049459 1107 6 4.670458256767521 1107 7 4.894694227555608 1107 8 5.232407124117081 1107 9 3.54819712100889 1107 10 3.047637776266143 1107 11 5.740965898469529 1107 12 3.0840799796159537 1107 13 4.484018881757084 1107 14 3.8767689626239346 1107 15 3.5689373787073215 1107 16 3.44180102887287 1107 17 3.991646824359499 1107 18 -1.0779954958924343 1107 19 1.600192158319441 1107 20 2.1175645597429202 1107 21 -1.3680952810247042 1107 22 1.7778031282913993 1107 23 -1.3395864354261775 1107 24 2.221055105061854 1107 25 3.0680179070526385 1108 1 5.284470321894823 1108 2 10.511019310144926 1108 3 0.26739841705774126 1108 4 5.684220381384925 1108 5 7.22145279396884 1108 6 7.445824193983714 1108 7 7.174375622205442 1108 8 6.813052285407405 1108 9 5.423136118006411 1108 10 4.642375843109274 1108 11 6.932784385853623 1108 12 2.6473000978405814 1108 13 3.797510483542414 1108 14 4.993851904083677 1108 15 4.962405128722676 1108 16 4.982656880797979 1108 17 0.3295727545946896 1108 18 3.5765804564815937 1108 19 4.69354210386113 1108 20 5.093367779408673 1108 21 5.1166870958138695 1108 22 2.2066962510310546 1108 23 4.657325439070849 1108 24 5.239374589501647 1108 25 5.856537869174215 1109 1 3.3787283658709555 1109 2 -13.79901653214898 1109 3 -5.63846934644474 1109 4 2.289634210964575 1109 5 0.5304154214451913 1109 6 2.5854371177495175 1109 7 3.747919079703056 1109 8 4.05284647464239 1109 9 3.3008538162944028 1109 10 2.2072616920786814 1109 11 0.6037978292813371 1109 12 1.1094140742812044 1109 13 2.622358230818296 1109 14 3.1515400247766934 1109 15 3.232626755284997 1109 16 3.1393180016655897 1109 17 3.5108484937778144 1109 18 3.634595676619007 1109 19 2.3136497334428334 1109 20 1.488041244814184 1109 21 0.2997908946862218 1109 22 2.954092806473639 1109 23 3.3037037241865406 1109 24 4.580099754078368 1109 25 4.7875876683957515 1110 1 3.58407698096169 1110 2 9.628872675109855 1110 3 6.686267998349912 1110 4 7.953435548118346 1110 5 8.86852430396668 1110 6 4.339973889478266 1110 7 6.857756711030657 1110 8 7.698500724881168 1110 9 5.735428792416656 1110 10 5.513851949958337 1110 11 3.2972075714016817 1110 12 6.008172431492284 1110 13 10.423695384590978 1110 14 8.027375745884623 1110 15 7.3854443293060115 1110 16 6.816490876546201 1110 17 7.113522801323182 1110 18 7.397728436886435 1110 19 6.30887418425468 1110 20 6.638948599159935 1110 21 4.796448010254512 1110 22 6.705764248689494 1110 23 7.310389493049961 1110 24 8.307893450353436 1110 25 8.575553284441593 1111 1 5.147592656614755 1111 2 9.59879368682912 1111 3 7.641937307254743 1111 4 8.018325501338879 1111 5 8.271115300836431 1111 6 7.680623921896228 1111 7 7.742502142886707 1111 8 7.68313705112438 1111 9 6.261750957239336 1111 10 5.411889617538286 1111 11 7.207089123838607 1111 12 6.546124104328818 1111 13 8.467447432499846 1111 14 6.547697102001159 1111 15 5.952744714835749 1111 16 5.7414964538299245 1111 17 6.901757695598793 1111 18 6.70725112597378 1111 19 5.76555699018612 1111 20 5.447732298153934 1111 21 7.970770370282121 1111 22 7.359088135498521 1111 23 7.261143820673553 1111 24 7.090609448094639 1111 25 7.522678020392071 1112 1 -1.438894821901453 1112 2 8.304891952121752 1112 3 6.069311657522288 1112 4 -0.15529309962276017 1112 5 4.634647131638097 1112 6 3.4192662159127742 1112 7 5.869164309844464 1112 8 6.035076332887339 1112 9 4.661513852732433 1112 10 3.572401366649359 1112 11 0.25136138869982005 1112 12 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2204 6 4.331933007511882 2204 7 5.313501927998416 2204 8 5.358712917089541 2204 9 3.93617873051139 2204 10 3.3507512987965598 2204 11 5.223819217211034 2204 12 5.040937324811139 2204 13 3.049210694920042 2204 14 4.316164497901646 2204 15 4.659269693866808 2204 16 4.568333748732911 2204 17 -1.0045570741587966 2204 18 2.66364353279198 2204 19 3.5580000274588386 2204 20 4.194515039158084 2204 21 5.457035715732381 2204 22 5.105288598548135 2204 23 3.918315544146978 2204 24 5.476937007171589 2204 25 5.534393764772048 2205 1 5.982213226089339 2205 2 10.30999247686003 2205 3 7.041315882910691 2205 4 7.4955300781784935 2205 5 7.8224699401472755 2205 6 7.1972675728464495 2205 7 7.516717964547787 2205 8 7.08105577276573 2205 9 6.009385270384595 2205 10 5.468966950345543 2205 11 7.704938798330616 2205 12 6.504357154897637 2205 13 9.628659374368723 2205 14 7.463855778926259 2205 15 6.254408422338869 2205 16 5.69644647684564 2205 17 6.488702852434464 2205 18 6.386548489030272 2205 19 4.790820026945507 2205 20 5.091309269285649 2205 21 -0.6001394081094098 2205 22 4.0024678290606985 2205 23 5.173483532648298 2205 24 6.958821455722706 2205 25 7.141246771625056 2206 1 4.089296909407025 2206 2 8.418533742074056 2206 3 5.636542919819629 2206 4 1.4234316543433216 2206 5 5.564664053822192 2206 6 5.717647367632093 2206 7 5.976325652674671 2206 8 5.876831255744136 2206 9 4.6334049024481025 2206 10 3.5228381890431066 2206 11 3.331323501132136 2206 12 4.481141251608609 2206 13 7.662936430217989 2206 14 0.29450617337921514 2206 15 2.176713542815836 2206 16 2.9465115327607876 2206 17 4.237549139306346 2206 18 -1.6828011165167744 2206 19 0.6157734996137876 2206 20 2.2557278011630473 2206 21 3.019047969396066 2206 22 -2.1789311201627477 2206 23 -3.263366114073378 2206 24 0.6353058011284389 2206 25 2.230485370370051 2207 1 1.3349935926628085 2207 2 -13.79901653214898 2207 3 -6.188320650174118 2207 4 -4.619474581653755 2207 5 0.883078824894092 2207 6 1.5191998499511392 2207 7 2.7268763988441513 2207 8 2.7897230607433627 2207 9 0.35241105478969104 2207 10 0.09021578538748187 2207 11 -1.5603920497588533 2207 12 -2.382160397137156 2207 13 0.06944843929571931 2207 14 -5.355280598830806 2207 15 -1.5731156534610662 2207 16 -0.49448238304873726 2207 17 0.48711850947081103 2207 18 0.510841747806289 2207 19 -0.07128000412004099 2207 20 -0.32776497737480526 2207 21 -3.7106084978888454 2207 22 -4.7129717484478455 2207 23 -1.7023785363172195 2207 24 0.05130359303242926 2207 25 0.7117966318300866 2208 1 5.08082092469704 2208 2 6.560163503501643 2208 3 6.514235861713022 2208 4 8.263049469227294 2208 5 3.518129235806569 2208 6 6.42879910437798 2208 7 6.6815057469826264 2208 8 6.8366851989833926 2208 9 5.187185530537796 2208 10 4.730151129176782 2208 11 6.75979740024804 2208 12 6.253063615368802 2208 13 8.691828425773068 2208 14 3.0912634004515276 2208 15 4.879407217925902 2208 16 5.260458617328371 2208 17 6.069649039263005 2208 18 0.43570299112595023 2208 19 3.295165548938087 2208 20 4.202356145038404 2208 21 6.990731141584671 2208 22 6.230487292374278 2208 23 1.9390228687758686 2208 24 3.311044802063698 2208 25 4.844036192061534 2209 1 2.717875884945915 2209 2 4.677219209295886 2209 3 4.664910580269645 2209 4 0.05643851513255882 2209 5 4.797856162871902 2209 6 5.195909370537677 2209 7 5.649027079746634 2209 8 4.401608645029629 2209 9 3.808297680893965 2209 10 2.65553051010302 2209 11 -1.4335259021112705 2209 12 2.588892831258409 2209 13 1.863863019078071 2209 14 3.3511198490800638 2209 15 3.6670809808289206 2209 16 3.1210619668603705 2209 17 0.9348646035391823 2209 18 2.9241927873209583 2209 19 3.3140464853430665 2209 20 3.3895913917122584 2209 21 5.300566240504985 2209 22 3.947446077270886 2209 23 0.11294504739498379 2209 24 -3.212858399036996 2209 25 -0.08900705634740547 2210 1 -1.3224767580124102 2210 2 -13.79901653214898 2210 3 -7.277921001032077 2210 4 -1.3325833293466793 2210 5 -0.020640817669363543 2210 6 -0.580024425033784 2210 7 -0.4422910444612249 2210 8 -20.76759287588292 2210 9 -12.647662954310688 2210 10 -6.544626054423933 2210 11 -9.410879961605792 2210 12 -4.000412320460075 2210 13 -4.594446084030853 2210 14 -3.4784335692454427 2210 15 -5.17879242556786 2210 16 -4.555673289980007 2210 17 -3.991848286940204 2210 18 -4.09495654541594 2210 19 -10.200912882434462 2210 20 -6.8512980439099 2210 21 -5.234135168575758 2210 22 -9.694379017786149 2210 23 -7.394666970796322 2210 24 -9.82594272021794 2210 25 -7.498922809720998 2211 1 0.3466690612348309 2211 2 4.9615198663184525 2211 3 0.9710197246834973 2211 4 1.590010765762628 2211 5 0.9767673409963997 2211 6 0.2008297557189742 2211 7 -20.65895771112789 2211 8 -12.896903220710504 2211 9 -8.713033700310238 2211 10 -6.987706964949062 2211 11 -9.458885370665968 2211 12 -4.730094538174747 2211 13 -6.542620526221523 2211 14 -4.681002872828945 2211 15 -5.1884723895074565 2211 16 -11.249452870777844 2211 17 -7.357579636951474 2211 18 -5.659268017131062 2211 19 -8.906321942610255 2211 20 -6.720252292275912 2211 21 -4.430371364203435 2211 22 -5.8922717380926635 2211 23 -7.727515742488908 2211 24 -21.85829566139441 2211 25 -21.647942417564135 2212 1 -0.2724477707047086 2212 2 -13.79901653214898 2212 3 -7.310183874502478 2212 4 -19.574740192091777 2212 5 -19.800052582375248 2212 6 -20.57120089562202 2212 7 -9.421365314084595 2212 8 -8.406452432377112 2212 9 -4.402017262031663 2212 10 -4.733156397923526 2212 11 -5.070656966388697 2212 12 -2.1347602095629163 2212 13 -5.2628167902859 2212 14 -2.762039176218959 2212 15 -2.7472221416992966 2212 16 -3.039027328533127 2212 17 -2.968875845587739 2212 18 -2.262049739610198 2212 19 -2.8366645854387897 2212 20 -2.9603379428684278 2212 21 -0.8198640958383194 2212 22 -2.1917919005739 2212 23 -2.1075290533273296 2212 24 -1.3028574354176428 2212 25 -1.8253447406897934 2213 1 -18.464633620757507 2213 2 -13.79901653214898 2213 3 -8.762381625672813 2213 4 -5.632286337585805 2213 5 -19.800052582375248 2213 6 -10.741667779443953 2213 7 -20.65895771112789 2213 8 -10.8273105740371 2213 9 -8.523185084987063 2213 10 -7.537565314818643 2213 11 -6.777888328470453 2213 12 -5.36373862818186 2213 13 -18.96503431932497 2213 14 -12.396061467310204 2213 15 -7.116293223932807 2213 16 -6.614020993882416 2213 17 -5.188214129694245 2213 18 -5.372282980876118 2213 19 -6.135121223751444 2213 20 -5.978033689308127 2213 21 -4.030453109432884 2213 22 -5.104965337004391 2213 23 -4.927470189803795 2213 24 -4.064593164108853 2213 25 -4.802122656434928 2214 1 -18.464633620757507 2214 2 -13.79901653214898 2214 3 -11.828262998296097 2214 4 -19.574740192091777 2214 5 -19.800052582375248 2214 6 -20.57120089562202 2214 7 -16.213608873918588 2214 8 -14.48387194565036 2214 9 -13.05272451381821 2214 10 -13.002560390949238 2214 11 -14.28632455804902 2214 12 -11.53848801585451 2214 13 -18.96503431932497 2214 14 -16.20919052318981 2214 15 -23.55820419027374 2214 16 -17.620356372515346 2214 17 -23.2761620124304 2214 18 -17.21789316192301 2214 19 -13.964501573724931 2214 20 -12.738598545505887 2214 21 -10.360332158999835 2214 22 -11.36298366894894 2214 23 -11.252852105315247 2214 24 -10.17341194046418 2214 25 -9.956081952447855 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 112938.0 3 6.0 4 561.0 5 2204.0 6 3017.0 7 2887.0 8 2507.0 9 2252.0 10 2169.0 11 1918.0 12 1863.0 13 1934.0 14 2981.0 15 6218.0 16 9506.0 17 9930.0 18 10125.0 19 11580.0 20 13823.0 21 16103.0 22 16885.0 23 18503.0 24 19227.0 25 19366.0 26 16238.0 27 12878.0 28 13753.0 29 15968.0 30 15609.0 31 18112.0 32 23020.0 33 28282.0 34 34704.0 35 40141.0 36 39169.0 37 40407.0 38 27454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.443125253770845 18.60271891965051 13.183113523918372 24.77104230266027 2 11.489817737109687 20.605013319112604 43.839493796805975 24.065675146971728 3 20.65321225165963 25.977684995327287 27.867844316652647 25.50125843636043 4 11.052543144055173 17.578382378761475 38.74988436392607 32.619190113257275 5 11.062976937625264 39.01201577011018 36.506487763109604 13.418519529154944 6 30.484113966228694 36.52040580562499 19.33152440619516 13.66395582195116 7 25.582310129537962 32.589430047346376 22.227291341863822 19.60096848125184 8 23.651993807493355 40.141568169529556 20.52403823291645 15.68239979006064 9 26.38022110919691 16.598999581121454 20.094435108867675 36.92634420081396 10 14.953542920522938 31.397084469070215 33.73671621757881 19.912656392828037 11 31.381924782468246 23.76861565675847 24.449066388428708 20.400393172344575 12 24.863192463918416 26.564860426929393 31.408866995073893 17.163080114078298 13 29.083032827292804 22.385209280299353 24.344482786729344 24.187275105678495 14 19.145541335910966 26.151493283369636 27.15468442761333 27.54828095310607 15 24.304096197874355 32.50203097829371 22.634665610334153 20.559207213497782 16 18.312306629671767 29.06004164446917 32.954253264923025 19.673398460936042 17 20.860404864821326 30.120211023706606 30.328617069831125 18.69076704164094 18 20.431596131612505 27.493536974460504 33.96334393550703 18.11152295841996 19 22.503256478242758 28.11117649781469 28.234823488961037 21.15074353498152 20 22.77230794350559 29.31602579802433 28.69654665686995 19.21511960160013 21 19.7225717793584 27.92230238858682 28.08798963305299 24.26713619900179 22 20.68250152321916 31.22715171262815 26.758655858433332 21.331690905719356 23 18.79767508573366 30.678958417545736 29.36997170463288 21.153394792087727 24 21.67547018305459 30.20875715379059 28.85336812917958 19.262404533975243 25 20.96691382010989 28.347940324586524 28.294967230028732 22.39017862527485 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1178.0 1 1178.0 2 2127.5 3 3077.0 4 3077.0 5 3077.0 6 3974.5 7 4872.0 8 4872.0 9 4872.0 10 5565.5 11 6259.0 12 6259.0 13 6259.0 14 7404.5 15 8550.0 16 8550.0 17 8550.0 18 11841.5 19 15133.0 20 15133.0 21 15133.0 22 20784.5 23 26436.0 24 26436.0 25 26436.0 26 34972.5 27 43509.0 28 43509.0 29 43509.0 30 52508.0 31 61507.0 32 61507.0 33 61507.0 34 65552.0 35 69597.0 36 69597.0 37 69597.0 38 71170.0 39 72743.0 40 72743.0 41 72743.0 42 74809.5 43 76876.0 44 76876.0 45 76876.0 46 82873.0 47 88870.0 48 88870.0 49 88870.0 50 75985.5 51 63101.0 52 63101.0 53 63101.0 54 48633.5 55 34166.0 56 34166.0 57 34166.0 58 26657.5 59 19149.0 60 19149.0 61 19149.0 62 14832.5 63 10516.0 64 10516.0 65 10516.0 66 7845.5 67 5175.0 68 5175.0 69 5175.0 70 3719.5 71 2264.0 72 2264.0 73 2264.0 74 1549.0 75 834.0 76 834.0 77 834.0 78 568.5 79 303.0 80 303.0 81 303.0 82 195.0 83 87.0 84 87.0 85 87.0 86 57.5 87 28.0 88 28.0 89 28.0 90 17.0 91 6.0 92 6.0 93 6.0 94 4.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.392528628967925 2 36.500021164434635 3 5.754284169979715 4 13.766487908595689 5 13.488745404875635 6 14.740051901705852 7 9.359564208010577 8 7.56302931437 9 0.11314832361397373 10 1.5093497960074107 11 5.756237810099668 12 5.810125716741719 13 16.116879776243085 14 1.6387784539543304 15 3.0063265379217827 16 3.1259869952689345 17 2.97702193612248 18 1.5702382464126283 19 1.5124430595306704 20 0.29109237787307196 21 4.3935738264321 22 1.6693854825002685 23 0.11559037376391562 24 9.16859588628512 25 0.11705560385388075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 614238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.26658701938668 #Duplication Level Percentage of deduplicated Percentage of total 1 96.16447515247046 79.1112316330436 2 2.433919061648613 4.0046042856651916 3 0.5325255848059638 1.314271870874688 4 0.24458459321443038 0.8048455888510492 5 0.13505389979516152 0.5555211699903092 6 0.09418465166282285 0.4648949905146143 7 0.05898611693107982 0.33968105650124775 8 0.040468313113393564 0.2663352001816623 9 0.030567153356991155 0.22631898434200654 >10 0.2159752790868058 3.4105899161252555 >50 0.024307449064552657 1.397305701073009 >100 0.020865657331298287 3.82825243156327 >500 0.0023662085633364494 1.5103838812623784 >1k 0.0017208789551537813 2.765763290011683 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 3882 0.6320025788049582 No Hit CCCATGTACTCTGCGTTGATACCAC 3637 0.5921157596892409 No Hit GAGTACATGGGAAGCAGTGGTATCA 1957 0.3186061428957505 No Hit CATGTACTCTGCGTTGATACCACTG 1346 0.2191333001214513 No Hit GCGTTGATACCACTGCTTCCCATGT 1339 0.21799367671814507 No Hit GTCCTACAGTGGACATTTCTAAATT 1236 0.20122493235521086 No Hit GTATCAACGCAGAGTACATGGGAAG 1136 0.18494459802226498 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1096 0.17843246428908663 No Hit CTGTAGGACGTGGAATATGGCAAGA 954 0.1553143895363035 No Hit ACTCTGCGTTGATACCACTGCTTCC 919 0.14961627251977247 No Hit GCTTCCCATGTACTCTGCGTTGATA 868 0.14131330200997008 No Hit GNACATGGGAAGCAGTGGTATCAAC 855 0.13919685854668712 No Hit CNCATGTACTCTGCGTTGATACCAC 842 0.13708041508340416 No Hit GTCCTAAAGTGTGTATTTCTCATTT 750 0.12210250749709395 No Hit GGTATCAACGCAGAGTACATGGGAA 737 0.11998606403381101 No Hit TATCAACGCAGAGTACATGGGAAGC 703 0.11445075036060941 No Hit CTTTAGGACGTGAAATATGGCGAGG 677 0.1102178634340435 No Hit GTCCTACAGTGTGCATTTCTCATTT 677 0.1102178634340435 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTACA 35 0.0057922397 19.128859 1 CTTATAC 35 8.340088E-4 18.77934 3 GGACGGG 40 1.3748372E-4 18.208082 6 TTCAACG 30 0.008551947 17.880877 4 AGGACGG 35 0.0011752484 17.84447 5 ATTAGCC 45 2.2820501E-4 17.040512 3 GTGTAGG 65 4.349823E-5 16.480247 1 TAGGACC 250 0.0 15.878219 4 GTTGTGA 70 8.196445E-5 15.303087 1 GTACTGG 70 8.196445E-5 15.303087 1 TGTAGGA 740 0.0 15.20764 2 CTAAGAC 65 1.4192508E-5 15.167929 3 TGTAGAA 285 0.0 14.446281 2 GATTAGA 45 8.6995837E-4 14.291283 11 CTGTAGG 710 0.0 14.144577 1 GATATAC 250 0.0 13.925809 1 GTAGGAC 750 0.0 13.875845 3 CGGGGAA 40 0.006578042 13.782241 9 CCTAGCA 80 2.069715E-4 13.723968 2 TCTAGGA 80 2.069715E-4 13.723968 2 >>END_MODULE