FastQCFastQC Report
Thu 26 May 2016
SRR1926553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614238
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC106691.736948869981994No Hit
GTACATGGGAAGCAGTGGTATCAAC97851.5930307144787528No Hit
GAGTACATGGGAAGCAGTGGTATCA47500.7733158808149284No Hit
CATGTACTCTGCGTTGATACCACTG38460.6261416584450978No Hit
GCGTTGATACCACTGCTTCCCATGT32920.5359486062405777No Hit
GTATCAACGCAGAGTACATGGGAAG30400.4949221637215542No Hit
ACGCAGAGTACATGGGAAGCAGTGG30050.48922404670502306No Hit
CTGTAGGACGTGGAATATGGCAAGA28310.46089626496569736No Hit
GTCCTACAGTGGACATTTCTAAATT25620.41710216561007296No Hit
ACTCTGCGTTGATACCACTGCTTCC24400.397240157723879No Hit
GCTTCCCATGTACTCTGCGTTGATA20700.33700292069197935No Hit
TATCAACGCAGAGTACATGGGAAGC18920.30802392557933567No Hit
CTTTAGGACGTGAAATATGGCGAGG18640.30346543196611087No Hit
GGTATCAACGCAGAGTACATGGGAA18270.29744170826292093No Hit
GTATCAACGCAGAGTACTTTTTTTT17340.28230099733328123No Hit
GTCCTAAAGTGTGTATTTCTCATTT16830.27399802682347885No Hit
GTCCTACAGTGTGCATTTCTCATTT16000.26048534932713374No Hit
CAGTGGTATCAACGCAGAGTACATG14930.24306539159088172No Hit
CTGTAGGACCTGGAATATGGCGAGA14130.23004112412452504No Hit
CATGGGAAGCAGTGGTATCAACGCA12480.20317857247516435No Hit
GATATACACTGTTCTACAAATCCCG12170.19813166883195116No Hit
ACATGGGAAGCAGTGGTATCAACGC11800.19210794512876117No Hit
GGTATCAACGCAGAGTACTTTTTTT11330.18445618799227662No Hit
ATACCACTGCTTCCCATGTACTCTG11090.1805489077523696No Hit
GTGGTATCAACGCAGAGTACATGGG10910.17761844757243936No Hit
CTGAAGGACCTGGAATATGGCGAGA10900.1774556442291099No Hit
GCAGTGGTATCAACGCAGAGTACAT10340.1683386570026602No Hit
CCACTGCTTCCCATGTACTCTGCGT9650.15710522631292756No Hit
CCATGTACTCTGCGTTGATACCACT9510.15482597950631513No Hit
GGGAAGCAGTGGTATCAACGCAGAG9220.15010468254976084No Hit
TATCAACGCAGAGTACTTTTTTTTT9080.1478254357431484No Hit
GTGTATATCAATGAGTTACAATGAA8850.14408095884657088No Hit
CTGCTTCCCATGTACTCTGCGTTGA8740.14229012206994684No Hit
GTACTCTGCGTTGATACCACTGCTT8600.14001087526333442No Hit
GATACCACTGCTTCCCATGTACTCT8560.13935966189001656No Hit
AAGCAGTGGTATCAACGCAGAGTAC8500.13838284183003982No Hit
GTTCTACAGTGTGGTTTTTATCATT8260.13447556159013282No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8190.1333359381868266No Hit
TACCACTGCTTCCCATGTACTCTGC8110.13203351144019093No Hit
GCAGAGTACATGGGAAGCAGTGGTA7870.12812623120028394No Hit
ATGGGAAGCAGTGGTATCAACGCAG7140.11624158713723345No Hit
CTTCCCATGTACTCTGCGTTGATAC7130.11607878379390399No Hit
GTGTATATCAATGAGTTACAATGAG6920.11265991358398535No Hit
GATATACACTGTTCTACAATGCCGG6800.11070627346403186No Hit
CTGTAGAACATATTAGATGAGTGAG6770.1102178634340435No Hit
GTCCTTCAGTGTGCATTTCTCATTT6540.10647338653746594No Hit
GAATATGGCAAGAAAACTGAAAATC6420.10451974641751242No Hit
ACCTGGAATATGGCGAGAAAACTGA6260.1019148929242411No Hit
TTGTAGAACAGTGTATATCAATGAG6190.10077526952093488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT601.509221E-615.78645319
CCAACGA1550.015.27721119
TCGTTGG508.910709E-515.15746317
TCCAACG2200.015.07134118
TAGGACC4800.014.5067074
CCCTTAT400.005148015514.3021231
GGACGAG551.8921246E-413.8732736
TTCGTTG552.0041905E-413.779510516
CTAGAGT852.560173E-713.4663394
GTCCTAG1153.5470293E-1013.2657381
CCTAGAT655.230387E-513.20953753
TAAGTCA655.238445E-513.2073714
CAGGACT801.9048584E-613.1156534
TCTAGGA956.962364E-813.055042
AGGACTG905.128077E-712.7182095
GGACTGT603.950822E-412.7171676
GTCCTAT751.4207511E-512.7129981
CTCCTGG751.4207511E-512.7129981
CTCTAGG905.153652E-712.71299651
TGTAGGA13050.012.647192
Produced by FastQC (version 0.11.5)