FastQCFastQC Report
Thu 26 May 2016
SRR1926552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691997
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24410.3527471939907254No Hit
CCCATGTACTCTGCGTTGATACCAC22270.3218222044315221No Hit
GTCCTACAGTGGACATTTCTAAATT12360.17861349109894983No Hit
GAGTACATGGGAAGCAGTGGTATCA11260.16271746842833132No Hit
CTGTAGGACGTGGAATATGGCAAGA9060.13092542308709432No Hit
CATGTACTCTGCGTTGATACCACTG8510.12297741175178506No Hit
GTCCTAAAGTGTGTATTTCTCATTT8090.11690802127754889No Hit
GTCCTACAGTGTGCATTTCTCATTT7610.109971575021279No Hit
GCGTTGATACCACTGCTTCCCATGT7220.1043357124380597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA6250.017.8063182
CTGTAGG6450.016.820451
TAGGACC2850.016.606314
GTCCTAC7550.015.9664311
GATATAC2450.015.8541961
CCAACGT602.7307215E-715.57736819
CCCCTGT1055.325637E-815.30751
GTAGGAC7700.014.8476883
GTAGTAC450.004372289414.6573333
GTCCTAA3200.014.64975551
CTACGTC450.005052238214.3418134
TCCTACA8700.013.8973672
GGACCTG3950.013.7633486
TGCTAAG600.006710847413.7394432
AGGACCT4050.013.6912515
TAAGATA550.001203830513.6899124
TAGGACA2400.013.445454
GTGTTGG802.5451026E-413.3940621
GTACAAG600.00794242313.3940621
GTCCTAT802.5451026E-413.3940621
Produced by FastQC (version 0.11.5)