##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926552_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 691997 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.876243683137353 27.0 7.0 31.0 2.0 31.0 2 16.21175380818125 16.0 2.0 30.0 2.0 31.0 3 19.85691989994176 23.0 15.0 31.0 2.0 31.0 4 22.064085537943082 28.0 2.0 35.0 2.0 37.0 5 22.6910188917004 30.0 2.0 35.0 2.0 37.0 6 23.170698716901953 30.0 2.0 35.0 2.0 37.0 7 23.149182727670784 30.0 2.0 35.0 2.0 37.0 8 23.27804889327555 30.0 7.0 35.0 2.0 37.0 9 25.961840875032696 31.0 17.0 37.0 2.0 39.0 10 26.67978473895118 31.0 22.0 37.0 2.0 39.0 11 23.799608957842302 30.0 7.0 37.0 2.0 39.0 12 24.921187519599073 31.0 10.0 37.0 2.0 39.0 13 21.59777715799346 27.0 2.0 35.0 2.0 39.0 14 24.937352329562124 30.0 13.0 38.0 2.0 40.0 15 26.011174903937444 31.0 17.0 38.0 2.0 40.0 16 26.368958246928816 31.0 16.0 38.0 2.0 40.0 17 25.713289219461934 31.0 10.0 38.0 2.0 40.0 18 25.802744809587324 31.0 11.0 38.0 2.0 40.0 19 26.1942667381506 31.0 11.0 39.0 2.0 40.0 20 26.430922388391856 31.0 10.0 39.0 2.0 40.0 21 24.324794760670926 30.0 2.0 37.0 2.0 40.0 22 25.238360859945924 31.0 2.0 38.0 2.0 40.0 23 25.229415734461277 31.0 2.0 38.0 2.0 40.0 24 24.278917394150554 31.0 2.0 39.0 2.0 40.0 25 24.08507262314721 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.233927336652361 1101 2 10.048161343791898 1101 3 6.917392540674314 1101 4 8.192112546326506 1101 5 8.04426574347685 1101 6 7.546450164871775 1101 7 7.158065060364034 1101 8 5.737244931429718 1101 9 5.623871694456415 1101 10 5.201294490274002 1101 11 7.9109460331087575 1101 12 5.633067372981916 1101 13 9.83594047606396 1101 14 7.391332548510697 1101 15 6.574437875181722 1101 16 6.125648520469252 1101 17 2.1766179199791758 1101 18 4.641913571275801 1101 19 5.4298910990533145 1101 20 3.307011938579869 1101 21 0.6621855504183678 1101 22 4.283766610301889 1101 23 5.408048017396528 1101 24 6.654110154411619 1101 25 6.97393902470186 1102 1 5.836238318630084 1102 2 10.942567087115952 1102 3 7.24856133395782 1102 4 8.545095935961218 1102 5 8.531761294792783 1102 6 7.032554226155682 1102 7 7.389850251642862 1102 8 7.296769782799913 1102 9 6.341139589835034 1102 10 5.643035690631066 1102 11 8.319902619786625 1102 12 5.7495207945304685 1102 13 9.983431126534498 1102 14 7.63150460319013 1102 15 5.802209040707773 1102 16 6.0365143948325795 1102 17 7.017677377735641 1102 18 6.935631737659499 1102 19 5.798704511033549 1102 20 6.037754241627145 1102 21 5.465906692315343 1102 22 6.254362996511844 1102 23 6.593377278360709 1102 24 7.839884283782268 1102 25 7.709627389278083 1103 1 4.584419202793974 1103 2 9.080081022862144 1103 3 6.478466534450401 1103 4 8.006877379740956 1103 5 7.436373024865578 1103 6 7.048450813810614 1103 7 6.97284390495793 1103 8 6.547123550369886 1103 9 5.386896929328028 1103 10 4.680777698981583 1103 11 5.764027017725958 1103 12 4.657909931570771 1103 13 8.406004485031115 1103 14 6.6361469372545585 1103 15 5.803580390999603 1103 16 5.540240795147362 1103 17 2.9273700990862963 1103 18 2.0393384639796643 1103 19 3.9816668316905144 1103 20 4.546240291307626 1103 21 6.738137941905467 1103 22 2.2262167968718067 1103 23 4.58041575923686 1103 24 5.967895680125821 1103 25 2.746817068446237 1104 1 5.904486897065421 1104 2 10.525314277672992 1104 3 7.394827537100085 1104 4 7.881029856784782 1104 5 7.969549313225965 1104 6 7.315384958901525 1104 7 7.408835887776206 1104 8 7.458868578175451 1104 9 6.326048097663747 1104 10 5.302992033100139 1104 11 8.29331733938131 1104 12 5.161114294409195 1104 13 9.43419513332567 1104 14 7.3369375403717925 1104 15 6.888041902431979 1104 16 6.372933399333075 1104 17 6.725212344184019 1104 18 4.337820004024337 1104 19 5.051460055809464 1104 20 4.1985190568911115 1104 21 3.6513164532768876 1104 22 5.615009235765893 1104 23 6.036851555451285 1104 24 7.108984453561558 1104 25 6.4386812066171295 1105 1 3.3369348490379913 1105 2 9.38760648461715 1105 3 6.185634164918596 1105 4 5.480819239256679 1105 5 2.1304727135381576 1105 6 4.3995386848209534 1105 7 5.619422320773506 1105 8 2.694967510369459 1105 9 3.78317519995214 1105 10 3.366643718144015 1105 11 6.819772020335144 1105 12 3.9385852671263493 1105 13 4.902611832642201 1105 14 3.6182922920151697 1105 15 4.079645808806681 1105 16 4.6169636569456785 1105 17 5.132931393668773 1105 18 2.5881501304155847 1105 19 2.6125567595207713 1105 20 1.3827326927176529 1105 21 2.5396083161018552 1105 22 3.785793931168584 1105 23 1.0055491472388596 1105 24 4.078598314516675 1105 25 4.685379424452869 1106 1 6.189003721970419 1106 2 10.89280495858623 1106 3 7.681432575179066 1106 4 8.86820479877317 1106 5 8.453500401084614 1106 6 7.275816154087636 1106 7 7.063072323783093 1106 8 6.746244827465887 1106 9 5.871246607702812 1106 10 5.324988971480433 1106 11 7.468199898736909 1106 12 4.979390565626897 1106 13 4.2262509064461256 1106 14 5.325361175092283 1106 15 5.59006406058014 1106 16 5.356229060125834 1106 17 5.8388836852513215 1106 18 5.819346114203029 1106 19 5.64763842760431 1106 20 5.329383831255477 1106 21 5.559640684405267 1106 22 5.841668142503984 1106 23 6.248356059420395 1106 24 7.079333465912548 1106 25 6.12747323301728 1107 1 3.3486855893661343 1107 2 -13.765412539369615 1107 3 -5.740156736116722 1107 4 1.2318792926380588 1107 5 3.8873360816055857 1107 6 3.9849857572236402 1107 7 4.065856613550665 1107 8 4.569174732540294 1107 9 3.0620466570010407 1107 10 2.355958728607142 1107 11 4.862124554610091 1107 12 2.721325600632614 1107 13 4.163104115668073 1107 14 3.4509868451572565 1107 15 3.220768640942218 1107 16 2.875243334083514 1107 17 3.4479633058669954 1107 18 -1.3977946945264677 1107 19 1.0046974171761462 1107 20 1.342726821034045 1107 21 -2.070458269862673 1107 22 1.20176718953741 1107 23 -2.1044403561212697 1107 24 1.249093640629713 1107 25 2.106195481228564 1108 1 5.3610497522909135 1108 2 10.558357952433665 1108 3 0.4185952898152081 1108 4 5.945602490555 1108 5 7.19433052855938 1108 6 7.2763865064863325 1108 7 7.207386225368822 1108 8 7.020426160656243 1108 9 5.49499163136138 1108 10 4.716445749357998 1108 11 7.069965243294099 1108 12 2.3328448503071364 1108 13 3.8195273162419774 1108 14 4.848456090846341 1108 15 4.901698311213494 1108 16 4.846959995879089 1108 17 0.011922973215614974 1108 18 3.408307013898753 1108 19 4.518291752537625 1108 20 4.85886253854607 1108 21 5.034426550925378 1108 22 1.7628241281882069 1108 23 4.591877097448357 1108 24 5.271624660636622 1108 25 5.694892773786421 1109 1 3.459181237261248 1109 2 -13.765412539369615 1109 3 -5.568625979481535 1109 4 2.225905052206155 1109 5 1.4890427294423212 1109 6 2.615628134020568 1109 7 3.8514052901271363 1109 8 4.051153320907066 1109 9 3.484805482836226 1109 10 2.6851674545009843 1109 11 1.5907733865890954 1109 12 1.6466892953764507 1109 13 2.6337527752864887 1109 14 3.4062703922269897 1109 15 3.469722662364969 1109 16 3.1994049527162502 1109 17 3.794330250441945 1109 18 4.029563657039095 1109 19 2.767487545918385 1109 20 1.7541835182159495 1109 21 0.975195046271665 1109 22 3.190206519999922 1109 23 3.482333470645216 1109 24 4.501554644046344 1109 25 4.813395149851431 1110 1 3.1271568710872337 1110 2 8.816779241452304 1110 3 5.996677481596027 1110 4 7.258725665942379 1110 5 7.870652670935307 1110 6 3.4987815210832345 1110 7 6.243569697185574 1110 8 6.942753804940995 1110 9 5.043133333584599 1110 10 4.889129894877872 1110 11 2.877005440913681 1110 12 5.706497444064155 1110 13 9.958798594032231 1110 14 7.430574887163431 1110 15 6.7086879810989615 1110 16 6.311436191702125 1110 17 6.829447675663399 1110 18 6.65218754387854 1110 19 5.272592224129809 1110 20 5.669857934908073 1110 21 4.008879167139167 1110 22 5.922309306719534 1110 23 6.2909535627526445 1110 24 7.203117474469991 1110 25 7.410250397860079 1111 1 4.700273867205208 1111 2 9.266346418285108 1111 3 7.300679980368951 1111 4 8.031579956246247 1111 5 8.354134554831607 1111 6 7.492027025523019 1111 7 7.333073735381102 1111 8 7.497464661216931 1111 9 6.031169342687271 1111 10 5.174639800727679 1111 11 6.914788226153071 1111 12 6.366179073621069 1111 13 8.001227535177204 1111 14 6.148141037714499 1111 15 5.474980203668238 1111 16 5.250241354345473 1111 17 6.619681824072664 1111 18 6.309666014272544 1111 19 4.95383134777445 1111 20 4.76944351788072 1111 21 7.4545392224691405 1111 22 6.721132987055082 1111 23 6.837802766268776 1111 24 6.816853474648475 1111 25 7.329628716542867 1112 1 -2.325450610032881 1112 2 7.425579954375172 1112 3 5.594450168881096 1112 4 -0.28825769347814045 1112 5 3.7091317973762905 1112 6 2.7908842436716945 1112 7 5.489680897158152 1112 8 5.611974445429073 1112 9 3.887719861170371 1112 10 3.1637030265972292 1112 11 -0.9185976301386809 1112 12 3.427859138169975 1112 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2204 7 5.2979190122540665 2204 8 5.438335996721815 2204 9 3.9191036532193024 2204 10 3.1733993012705675 2204 11 4.644459778813225 2204 12 4.632148128995663 2204 13 2.966344256132686 2204 14 4.190095435108635 2204 15 4.386302136350103 2204 16 4.0714818538025845 2204 17 -1.338760086893675 2204 18 2.3269135712757993 2204 19 3.541952954723417 2204 20 3.6497095330815874 2204 21 5.327554966225925 2204 22 4.942400630920446 2204 23 3.815633928049447 2204 24 5.083414278122962 2204 25 5.176026653567842 2205 1 5.512905714636808 2205 2 10.114685339912603 2205 3 7.06288566501912 2205 4 7.439636133215391 2205 5 7.534403536876443 2205 6 7.161056097051411 2205 7 7.238388017148033 2205 8 6.864922456754442 2205 9 5.710586796231809 2205 10 5.09123915988938 2205 11 7.302495395996477 2205 12 6.376442582503003 2205 13 9.37976051496901 2205 14 7.087044864146154 2205 15 5.949612359297358 2205 16 5.360558797793885 2205 17 6.212368243720789 2205 18 6.11254978661022 2205 19 4.819582100998566 2205 20 4.910163584196326 2205 21 -1.269265316536444 2205 22 3.4625406526713967 2205 23 4.656108370681313 2205 24 6.45486751357701 2205 25 6.931509252806556 2206 1 4.281910340732058 2206 2 8.884012747986707 2206 3 6.153981168304533 2206 4 1.9358364134062214 2206 5 6.0118101377009445 2206 6 6.279505029192194 2206 7 6.436995366933871 2206 8 6.364883615769436 2206 9 5.035134030887612 2206 10 4.392007675654803 2206 11 3.6501289085340787 2206 12 5.034418243938191 2206 13 8.374304026247632 2206 14 0.8324435467670916 2206 15 2.961429394149775 2206 16 3.935993348055458 2206 17 5.011445167621922 2206 18 -0.3515182842250191 2206 19 1.5228396542308076 2206 20 3.1894878582047426 2206 21 4.008790598409835 2206 22 -1.5622586465836577 2206 23 -2.4969974348405337 2206 24 1.5139192042313354 2206 25 2.960603829265704 2207 1 1.596216220373691 2207 2 -13.765412539369615 2207 3 -5.996149906508936 2207 4 -3.9602399844850567 2207 5 1.5844198228333148 2207 6 2.161667331499686 2207 7 3.2818517607920796 2207 8 3.5048167820183913 2207 9 1.100484187889311 2207 10 0.8117639629247009 2207 11 -1.12188733898126 2207 12 -1.9374654883811147 2207 13 0.6021149328244171 2207 14 -5.141759201482845 2207 15 -1.010872049113555 2207 16 0.15990081787944277 2207 17 1.3049951344931259 2207 18 1.253442393330939 2207 19 0.7490373323341117 2207 20 0.5435482966571925 2207 21 -3.2793435300146747 2207 22 -4.001532117617565 2207 23 -0.8495310677734409 2207 24 0.9552668261597184 2207 25 1.357645283475943 2208 1 4.489961883967705 2208 2 6.019091092591642 2208 3 5.893050495626344 2208 4 7.74100465253121 2208 5 3.382743315180001 2208 6 5.791249762152589 2208 7 6.3399065021652845 2208 8 6.330581759433681 2208 9 4.582168624970716 2208 10 4.246367420721736 2208 11 6.228904895843087 2208 12 5.826464109625203 2208 13 8.207484288416786 2208 14 2.6009025376106543 2208 15 4.27042441237916 2208 16 4.619595251704119 2208 17 5.4534311963993005 2208 18 0.4925996084025144 2208 19 3.018595408315022 2208 20 4.035484351483525 2208 21 6.533323731359097 2208 22 5.6377497027525685 2208 23 1.528008827161635 2208 24 2.642819040027721 2208 25 4.251759099088421 2209 1 2.9078726646670034 2209 2 4.515156974868242 2209 3 4.9747929447565795 2209 4 0.3537799900744574 2209 5 4.658293787476918 2209 6 4.999484235568904 2209 7 5.609924874900635 2209 8 4.38235190961964 2209 9 4.03654502675364 2209 10 2.915648044989158 2209 11 -0.898785614821616 2209 12 2.5679479614914733 2209 13 1.6849665341896376 2209 14 3.100095435108635 2209 15 3.7356865584606567 2209 16 3.322465940904763 2209 17 1.2091042347143635 2209 18 3.1251631525153307 2209 19 3.6866430719763486 2209 20 3.570856936766681 2209 21 5.6936228054219065 2209 22 4.6670446845217874 2209 23 0.5623634087864673 2209 24 -2.6316485359473916 2209 25 0.3316338562479082 2210 1 -2.048850656146392 2210 2 -13.765412539369615 2210 3 -7.648981501993667 2210 4 -1.8090749558725712 2210 5 -0.585051779950664 2210 6 -1.3651146404951469 2210 7 -1.1945468580531013 2210 8 -20.754164003278184 2210 9 -13.035347427554305 2210 10 -7.147013099184495 2210 11 -9.84868299706846 2210 12 -4.555044703768843 2210 13 -5.22805563010167 2210 14 -3.9843755546524555 2210 15 -5.905418568996879 2210 16 -5.372644994889111 2210 17 -4.775591713742369 2210 18 -4.941704176164471 2210 19 -10.35291621478739 2210 20 -7.090080000479276 2210 21 -5.424950722056213 2210 22 -9.613301871923694 2210 23 -7.7494030457844545 2210 24 -10.371076620625617 2210 25 -8.08218438169951 2211 1 -0.428506264481161 2211 2 4.362902259603866 2211 3 -0.04132772130541085 2211 4 0.9645720455901312 2211 5 0.40613478889117616 2211 6 -0.5415277820326487 2211 7 -20.624580987745933 2211 8 -13.602752540489476 2211 9 -9.584321639053295 2211 10 -7.814112104489343 2211 11 -10.346370703080131 2211 12 -5.469834334648478 2211 13 -7.079694380879003 2211 14 -5.420434932727122 2211 15 -5.856909297924204 2211 16 -11.452623506904573 2211 17 -8.043971093451987 2211 18 -6.383749388518897 2211 19 -8.834357281946101 2211 20 -7.059536261044446 2211 21 -4.8755195989294755 2211 22 -6.250217703838608 2211 23 -8.112874055982488 2211 24 -21.785752388543706 2211 25 -21.623140013098826 2212 1 -1.6391102522004388 2212 2 -13.765412539369615 2212 3 -8.058973203335478 2212 4 -19.60685652583844 2212 5 -20.150751438653735 2212 6 -20.62279382138252 2212 7 -10.20677276856785 2212 8 -9.657417427935718 2212 9 -5.701729680114031 2212 10 -6.025253666765963 2212 11 -6.03052880566166 2212 12 -3.423639542237215 2212 13 -6.661920584049961 2212 14 -4.411890866261228 2212 15 -4.401757224380489 2212 16 -4.439419972681435 2212 17 -4.727059173651668 2212 18 -3.7776069766694036 2212 19 -4.354120104637314 2212 20 -4.58613521577686 2212 21 -2.4226276821759 2212 22 -3.63162904944485 2212 23 -3.7698341084802323 2212 24 -2.8859236214204174 2212 25 -3.3209139857015657 2213 1 -18.443048608364823 2213 2 -13.765412539369615 2213 3 -8.65926448878258 2213 4 -5.84394139668715 2213 5 -20.150751438653735 2213 6 -10.801760611050415 2213 7 -20.624580987745933 2213 8 -10.774459206230214 2213 9 -8.400991672733959 2213 10 -7.520878066503112 2213 11 -6.814282533609898 2213 12 -5.602473642222051 2213 13 -19.039489077200646 2213 14 -12.397137037216089 2213 15 -7.173793189603156 2213 16 -6.374351479530748 2213 17 -5.071632165614464 2213 18 -5.056425912119032 2213 19 -6.160974474550876 2213 20 -6.050997723990982 2213 21 -4.152171356037297 2213 22 -4.900142419510058 2213 23 -4.6470013733035636 2213 24 -3.6953464844846664 2213 25 -4.520741489113586 2214 1 -18.443048608364823 2214 2 -13.765412539369615 2214 3 -12.489616768396852 2214 4 -19.60685652583844 2214 5 -20.150751438653735 2214 6 -20.62279382138252 2214 7 -16.60067246383741 2214 8 -15.127344876459057 2214 9 -14.153392881777233 2214 10 -14.159187323816058 2214 11 -14.475278613425168 2214 12 -12.638449584102052 2214 13 -19.039489077200646 2214 14 -16.781401446388244 2214 15 -23.47453119698323 2214 16 -18.21946582464509 2214 17 -23.19709342022701 2214 18 -17.72918829982607 2214 19 -15.191089318318856 2214 20 -13.895283536911482 2214 21 -11.417727431556472 2214 22 -12.466547740527925 2214 23 -12.417628372712853 2214 24 -11.283673386464704 2214 25 -11.11690300686182 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 129077.0 3 4.0 4 653.0 5 2446.0 6 3319.0 7 3204.0 8 2811.0 9 2578.0 10 2357.0 11 2132.0 12 2088.0 13 2236.0 14 3369.0 15 6870.0 16 10284.0 17 11072.0 18 11428.0 19 13345.0 20 15645.0 21 18075.0 22 18638.0 23 20246.0 24 21204.0 25 21563.0 26 18143.0 27 14385.0 28 14907.0 29 17757.0 30 16939.0 31 19776.0 32 25106.0 33 31572.0 34 38893.0 35 45039.0 36 44235.0 37 46580.0 38 34021.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99997995231993 17.879022274643194 12.60915544135803 25.511842331678842 2 12.905964520440108 20.020183805356716 43.05260604507259 24.021245629130586 3 20.358444258100715 24.55332437043839 27.7762873686034 27.311944002857498 4 11.391301870253765 16.448143573275768 37.39451644747317 34.7660381089973 5 11.825888949625908 38.28528215326521 36.72536545613688 13.163463440972 6 31.98019162377005 36.33082640300832 18.00671839193793 13.682263581283701 7 27.54105948637735 30.8331604232838 22.009161572470592 19.616618517868257 8 24.415317237929624 39.18013482004394 20.449901919123654 15.954646022902786 9 26.55433106405386 15.721390731734134 19.534850751234696 38.18942745297731 10 14.666826929419404 30.201271450412115 34.13332786094666 20.998573759221824 11 33.02108949082279 22.45120104006329 24.73201199506951 19.79569747404441 12 24.57943581394921 25.01341329188243 31.543157075013262 18.8639938191551 13 29.174989114583695 21.61774564756132 24.88285218641362 24.32441305144137 14 19.667633536689195 24.073889224879483 27.07505181757864 29.18342542085269 15 23.516053086651247 32.26749290878299 22.506168540295672 21.710285464270086 16 20.07512346819019 28.194385697349528 30.818792186206117 20.911698648254166 17 20.968768968923694 29.150102952903513 29.631392899225172 20.24973517894762 18 20.895971180600885 27.080525050494085 32.452039785086605 19.571463983818425 19 23.220219972235945 26.948431272235794 27.466625230695257 22.364723524832996 20 23.17981652173787 28.269012576274182 28.351753128853556 20.19941777313439 21 21.04647189217142 26.972870479295256 27.258651295993324 24.722006332540005 22 21.613525193801294 29.749141312312677 27.035409211123362 21.60192428276266 23 20.35966518819421 29.162652223350605 28.684390976399417 21.79329161205577 24 22.515163910291406 28.22405184776087 28.712034518487155 20.54874972346057 25 21.77366270027849 27.609099786610724 28.255633115121704 22.361604397989076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 945.0 1 945.0 2 1779.5 3 2614.0 4 2614.0 5 2614.0 6 3654.5 7 4695.0 8 4695.0 9 4695.0 10 5441.0 11 6187.0 12 6187.0 13 6187.0 14 7350.5 15 8514.0 16 8514.0 17 8514.0 18 11874.0 19 15234.0 20 15234.0 21 15234.0 22 21257.5 23 27281.0 24 27281.0 25 27281.0 26 35827.0 27 44373.0 28 44373.0 29 44373.0 30 54273.0 31 64173.0 32 64173.0 33 64173.0 34 69603.5 35 75034.0 36 75034.0 37 75034.0 38 78024.5 39 81015.0 40 81015.0 41 81015.0 42 82949.5 43 84884.0 44 84884.0 45 84884.0 46 88165.0 47 91446.0 48 91446.0 49 91446.0 50 81536.0 51 71626.0 52 71626.0 53 71626.0 54 59545.0 55 47464.0 56 47464.0 57 47464.0 58 39232.0 59 31000.0 60 31000.0 61 31000.0 62 24824.0 63 18648.0 64 18648.0 65 18648.0 66 14191.5 67 9735.0 68 9735.0 69 9735.0 70 7057.5 71 4380.0 72 4380.0 73 4380.0 74 3078.0 75 1776.0 76 1776.0 77 1776.0 78 1227.0 79 678.0 80 678.0 81 678.0 82 453.0 83 228.0 84 228.0 85 228.0 86 139.5 87 51.0 88 51.0 89 51.0 90 32.0 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.50063656345331 2 36.8517493572949 3 5.733839886589104 4 13.777516376516083 5 13.817834470380653 6 14.994429166600435 7 9.460590147067112 8 7.8407854369310845 9 0.10621433329913281 10 1.413878961903014 11 5.74120986073639 12 5.731094209945997 13 16.364377302213736 14 1.5550645450775076 15 2.8958218026956764 16 2.972411730108657 17 2.8677869990765856 18 1.4789081455555444 19 1.4190812965952164 20 0.2728335527466159 21 4.429354462519346 22 1.5919144158139416 23 0.10737040767517779 24 9.204664182070154 25 0.11156117728834083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 691997.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.93994345736508 #Duplication Level Percentage of deduplicated Percentage of total 1 96.14968566187403 83.59248234886755 2 2.5756671748061652 4.478567170852785 3 0.5441023428510989 1.419126807674832 4 0.23769808805484882 0.8266183334164937 5 0.1247719250191339 0.5423832053115049 6 0.0776359482647577 0.4049798971438173 7 0.04873339322515754 0.29658149160365144 8 0.035027300813535826 0.2436217241754338 9 0.02223219022494781 0.17395788249813124 >10 0.15277608127522707 2.470447812608032 >50 0.01513132352431355 0.9118327984354593 >100 0.014075378781184818 2.488266679439118 >500 0.0017594223468787844 1.0757963701055864 >1k 7.037689387515137E-4 1.0753374778675207 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2441 0.3527471939907254 No Hit CCCATGTACTCTGCGTTGATACCAC 2227 0.3218222044315221 No Hit GTCCTACAGTGGACATTTCTAAATT 1236 0.17861349109894983 No Hit GAGTACATGGGAAGCAGTGGTATCA 1126 0.16271746842833132 No Hit CTGTAGGACGTGGAATATGGCAAGA 906 0.13092542308709432 No Hit CATGTACTCTGCGTTGATACCACTG 851 0.12297741175178506 No Hit GTCCTAAAGTGTGTATTTCTCATTT 809 0.11690802127754889 No Hit GTCCTACAGTGTGCATTTCTCATTT 761 0.109971575021279 No Hit GCGTTGATACCACTGCTTCCCATGT 722 0.1043357124380597 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4450929700562286E-4 0.0 5 0.0 0.0 0.0 1.4450929700562286E-4 0.0 6 0.0 0.0 0.0 1.4450929700562286E-4 0.0 7 0.0 0.0 0.0 1.4450929700562286E-4 0.0 8 0.0 0.0 0.0 2.890185940112457E-4 0.0 9 0.0 0.0 0.0 2.890185940112457E-4 0.0 10 0.0 0.0 0.0 2.890185940112457E-4 0.0 11 0.0 0.0 0.0 2.890185940112457E-4 0.0 12 0.0 0.0 0.0 2.890185940112457E-4 0.0 13 0.0 0.0 0.0 2.890185940112457E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 625 0.0 17.806318 2 CTGTAGG 645 0.0 16.82045 1 TAGGACC 285 0.0 16.60631 4 GTCCTAC 755 0.0 15.966431 1 GATATAC 245 0.0 15.854196 1 CCAACGT 60 2.7307215E-7 15.577368 19 CCCCTGT 105 5.325637E-8 15.3075 1 GTAGGAC 770 0.0 14.847688 3 GTAGTAC 45 0.0043722894 14.657333 3 GTCCTAA 320 0.0 14.6497555 1 CTACGTC 45 0.0050522382 14.341813 4 TCCTACA 870 0.0 13.897367 2 GGACCTG 395 0.0 13.763348 6 TGCTAAG 60 0.0067108474 13.739443 2 AGGACCT 405 0.0 13.691251 5 TAAGATA 55 0.0012038305 13.689912 4 TAGGACA 240 0.0 13.44545 4 GTGTTGG 80 2.5451026E-4 13.394062 1 GTACAAG 60 0.007942423 13.394062 1 GTCCTAT 80 2.5451026E-4 13.394062 1 >>END_MODULE