FastQCFastQC Report
Thu 26 May 2016
SRR1926552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691997
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC64600.9335300586563237No Hit
GTACATGGGAAGCAGTGGTATCAAC61710.8917668718216986No Hit
GAGTACATGGGAAGCAGTGGTATCA26330.380492979015805No Hit
GTCCTACAGTGGACATTTCTAAATT26100.37716926518467564No Hit
CTGTAGGACGTGGAATATGGCAAGA25880.37399006065055196No Hit
CATGTACTCTGCGTTGATACCACTG22880.33063727154886513No Hit
GTCCTACAGTGTGCATTTCTCATTT18440.26647514367836855No Hit
ACGCAGAGTACATGGGAAGCAGTGG18420.2661861250843573No Hit
GCGTTGATACCACTGCTTCCCATGT18160.26242888336221115No Hit
GTCCTAAAGTGTGTATTTCTCATTT17070.24667736998859824No Hit
CTTTAGGACGTGAAATATGGCGAGG17030.24609933280057575No Hit
GTATCAACGCAGAGTACTTTTTTTT16510.23858484935628332No Hit
GTATCAACGCAGAGTACATGGGAAG16440.23757328427724397No Hit
CTGTAGGACCTGGAATATGGCGAGA16140.23323800536707529No Hit
ACTCTGCGTTGATACCACTGCTTCC13690.19783322760069771No Hit
GCTTCCCATGTACTCTGCGTTGATA11680.1687868589025675No Hit
GATATACACTGTTCTACAAATCCCG11040.15953826389420764No Hit
GGTATCAACGCAGAGTACTTTTTTT10590.1530353455289546No Hit
GGTATCAACGCAGAGTACATGGGAA10270.14841104802477467No Hit
TATCAACGCAGAGTACATGGGAAGC9920.14335322262957786No Hit
CTGAAGGACCTGGAATATGGCGAGA9900.14306420403556663No Hit
CAGTGGTATCAACGCAGAGTACATG8860.12803523714698184No Hit
TATCAACGCAGAGTACTTTTTTTTT8050.1163299840895264No Hit
GTGTATATCAATGAGTTACAATGAA7430.10737040767517779No Hit
CATGGGAAGCAGTGGTATCAACGCA7350.10621433329913281No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7190.10390218454704282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4850.015.3429654
AGCACCG400.00512947814.31026655
CTATTAC400.0051418814.305061
TGTAGGA14050.013.8569742
ATAGGAC1450.013.8188213
CTGTAGG14050.013.6432611
GTAGGAC13600.013.4704473
ACTGTTC4500.012.876018
TATAGGA1501.8189894E-1212.7248682
AATGCCG1501.8189894E-1212.62923318
GATATAC5050.012.4638151
GTCCTAC13650.012.4361451
ATGCCGG1457.2759576E-1212.41148919
GGCGAGG3750.012.37664919
AGGACGT10750.012.3356715
TAGGACA3500.012.2659434
TTGGAGT550.002970064512.1420444
GGACGTG10750.012.0694346
CGAAATC550.00311388812.0656413
TCCAACG1650.012.05517818
Produced by FastQC (version 0.11.5)