FastQCFastQC Report
Thu 26 May 2016
SRR1926551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664452
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24260.3651129050706447No Hit
CCCATGTACTCTGCGTTGATACCAC21980.33079891399228234No Hit
GTCCTACAGTGGACATTTCTAAATT12600.18962995069621283No Hit
GAGTACATGGGAAGCAGTGGTATCA10980.16524895703527118No Hit
CTGTAGGACGTGGAATATGGCAAGA8700.13093496595690887No Hit
GCGTTGATACCACTGCTTCCCATGT7770.1169384695959979No Hit
CATGTACTCTGCGTTGATACCACTG7710.11603546983077785No Hit
GTCCTAAAGTGTGTATTTCTCATTT7420.11167097096554754No Hit
GTCCTACAGTGTGCATTTCTCATTT7360.11076797120032748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAA350.005866804619.087011
CGTAGCG300.007515080318.2865663
CTGTAGG6450.017.8145431
TGTAGGA7050.017.4820142
CCTATAC657.4896343E-716.8799063
TGTAAGA653.5891968E-516.8544522
TTGGACT452.684044E-416.6826864
CGCAGAC504.690179E-516.3384657
CAGACTA754.836693E-915.98588859
TGTAGAA2700.015.723022
CCTACTC505.0319964E-415.3607143
TATACTG901.03809725E-815.0877535
CTAGACT505.9835316E-415.0144184
CTATAGT1001.8153514E-915.0144184
CATAGGA550.003850045414.9391742
TGTAGGG550.003850045414.9391742
GATATAC2800.014.7924331
GTAGGAC7800.014.76991753
AGTGTTC400.00436295314.66235358
GTCCTAC6750.014.6475121
Produced by FastQC (version 0.11.5)