##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926551_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 664452 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.450637517834245 27.0 10.0 31.0 2.0 31.0 2 16.451746702545858 18.0 2.0 30.0 2.0 31.0 3 20.158712743734686 25.0 15.0 31.0 2.0 31.0 4 22.106088927416877 28.0 7.0 35.0 2.0 37.0 5 23.079543744318627 30.0 7.0 35.0 2.0 37.0 6 23.695285739225707 31.0 2.0 35.0 2.0 37.0 7 23.518916340081752 30.0 7.0 35.0 2.0 37.0 8 23.73588761866922 31.0 11.0 35.0 2.0 37.0 9 26.556294510363426 32.0 17.0 37.0 2.0 39.0 10 27.312976708626056 32.0 25.0 37.0 2.0 39.0 11 24.31927964698729 30.0 8.0 37.0 2.0 39.0 12 25.514255356293607 31.0 16.0 37.0 2.0 39.0 13 22.082519128545027 28.0 2.0 37.0 2.0 39.0 14 25.55736607008482 31.0 17.0 38.0 2.0 40.0 15 26.70500803669791 31.0 17.0 38.0 2.0 40.0 16 27.07151758140543 32.0 18.0 39.0 2.0 40.0 17 26.401592590585928 31.0 17.0 38.0 2.0 40.0 18 26.495540686159423 31.0 17.0 38.0 2.0 40.0 19 26.89378465261599 31.0 18.0 39.0 2.0 40.0 20 27.15120580568649 31.0 22.0 39.0 2.0 40.0 21 24.957155671139525 30.0 8.0 38.0 2.0 40.0 22 25.927645638812134 31.0 10.0 38.0 2.0 40.0 23 25.932204282626888 31.0 2.0 38.0 2.0 40.0 24 24.9468238488258 31.0 2.0 39.0 2.0 40.0 25 24.740535057460885 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.942736785241625 1101 2 10.960674685398619 1101 3 7.437700304399016 1101 4 9.126736401159565 1101 5 8.720065527977997 1101 6 8.13689668861279 1101 7 7.789027209587893 1101 8 6.219511876010564 1101 9 6.390114480749542 1101 10 6.054045782369492 1101 11 8.764586114463786 1101 12 6.210174203433841 1101 13 10.852829007926221 1101 14 8.330622106855525 1101 15 7.389152343623195 1101 16 6.825414859049616 1101 17 2.3490660518588022 1101 18 5.180730934626759 1101 19 5.737595925163625 1101 20 4.193118123979122 1101 21 1.149865195448438 1101 22 5.083464098365017 1101 23 5.963896897046755 1101 24 7.495050716194569 1101 25 7.900236840066736 1102 1 5.653532239787079 1102 2 10.676577532551466 1102 3 6.8993636410623544 1102 4 8.238112525035689 1102 5 7.937679164341635 1102 6 6.735966368932473 1102 7 7.031940546251231 1102 8 7.093281856030547 1102 9 5.964527567662632 1102 10 5.324194383768095 1102 11 8.10680015042782 1102 12 5.244396231405869 1102 13 9.775194142791356 1102 14 7.3961315973650095 1102 15 5.330123872094724 1102 16 5.964856667241428 1102 17 6.793696421489173 1102 18 6.645740924636748 1102 19 5.337864406682105 1102 20 5.952240251851254 1102 21 4.727793517126763 1102 22 5.922462599863518 1102 23 6.369716077865938 1102 24 8.020169347563197 1102 25 7.715971105801 1103 1 4.994565324800224 1103 2 9.254256103980037 1103 3 6.477803606005384 1103 4 7.844061254220616 1103 5 7.319619348390713 1103 6 7.020437227828179 1103 7 6.934191860887516 1103 8 6.483712399413541 1103 9 5.389823360826522 1103 10 4.691268222506043 1103 11 5.029296863837111 1103 12 4.161130455770461 1103 13 8.555069252344381 1103 14 6.738528617522768 1103 15 5.744201435635482 1103 16 5.488558463911012 1103 17 3.3983633802972335 1103 18 2.149268531998711 1103 19 4.014469358478774 1103 20 4.613935037127323 1103 21 6.603335804593897 1103 22 2.7434858251045355 1103 23 4.716582861389163 1103 24 5.977208129333576 1103 25 2.1190824116730447 1104 1 6.039268302504848 1104 2 10.819587114432998 1104 3 7.482987334442143 1104 4 7.799828552910256 1104 5 7.942666098139544 1104 6 7.509289539664181 1104 7 7.499383926042171 1104 8 7.424270531988732 1104 9 6.548456139091204 1104 10 5.324869470876109 1104 11 8.373720882135135 1104 12 5.562623583322242 1104 13 10.005551285648501 1104 14 7.557194315135046 1104 15 6.972066380805529 1104 16 6.426289594070695 1104 17 6.559288755983943 1104 18 4.2918210640095715 1104 19 5.3236440234068425 1104 20 4.393289903419195 1104 21 3.9484598934333803 1104 22 5.833655979654459 1104 23 6.329385067412975 1104 24 7.386001229096298 1104 25 6.313183649355011 1105 1 3.83187776474405 1105 2 9.697457480916356 1105 3 6.679914415589032 1105 4 5.819674487169941 1105 5 2.5775995602108246 1105 6 4.783438399913539 1105 7 6.333091578953468 1105 8 3.5648502725537803 1105 9 4.536407946320118 1105 10 4.255057120429022 1105 11 7.3547670178977 1105 12 4.907896661698466 1105 13 5.606239753806847 1105 14 4.410202165351244 1105 15 4.844689276569767 1105 16 5.113813207688931 1105 17 5.6593160428316835 1105 18 3.0571436785093837 1105 19 2.9772899660624823 1105 20 2.2319196838650193 1105 21 2.9351837925140245 1105 22 4.4943860077809035 1105 23 1.9992771794149924 1105 24 5.206884063570083 1105 25 5.771823085146959 1106 1 6.288175710577455 1106 2 11.327648347475375 1106 3 7.81652122574571 1106 4 9.323084297249732 1106 5 8.597838099049536 1106 6 7.7181122943129346 1106 7 7.60899933368373 1106 8 7.316002155490537 1106 9 6.281764926523703 1106 10 5.734052631190771 1106 11 8.142106360540733 1106 12 5.308163413536452 1106 13 4.306115841367696 1106 14 5.7460211408112585 1106 15 6.033419159281763 1106 16 5.936257624531557 1106 17 6.430404816187256 1106 18 6.441604940346124 1106 19 6.1189671312770955 1106 20 5.908741847334806 1106 21 6.317333895133633 1106 22 6.472726468297488 1106 23 6.856424472564022 1106 24 7.735648606276996 1106 25 6.85153811611028 1107 1 3.7811032380659064 1107 2 -14.031458181734248 1107 3 -5.888071815047802 1107 4 1.5098638159134872 1107 5 4.190336221140257 1107 6 4.562268004044348 1107 7 4.710027895648821 1107 8 5.02182101265705 1107 9 3.08546130260239 1107 10 2.644902215093392 1107 11 5.3349735583452045 1107 12 2.8295834086864176 1107 13 4.625172628161408 1107 14 3.61760319805348 1107 15 3.252606660390768 1107 16 3.0329181990830776 1107 17 3.686854769165592 1107 18 -1.4222022767401867 1107 19 1.5556032190745306 1107 20 1.8463775151903228 1107 21 -1.472733591306973 1107 22 1.6200314466793522 1107 23 -1.4585541458862146 1107 24 2.121686128974556 1107 25 2.9172619835979035 1108 1 5.162724643793755 1108 2 10.65635483996859 1108 3 0.3369977932207142 1108 4 5.891830030401778 1108 5 7.115193772021101 1108 6 7.20521422249794 1108 7 7.085027536473028 1108 8 6.8512728529301405 1108 9 5.262384114287961 1108 10 4.186537872916674 1108 11 6.89511251627566 1108 12 2.6386053299033634 1108 13 3.3756919963568244 1108 14 4.760852560875627 1108 15 4.586148794584588 1108 16 4.528550621450822 1108 17 -0.18721819811493035 1108 18 3.4396414731700204 1108 19 4.430061222804213 1108 20 4.77430530074226 1108 21 5.002637006275343 1108 22 1.8100699215902054 1108 23 4.240470907343639 1108 24 5.2374973346845906 1108 25 5.590845897119866 1109 1 2.849788836093147 1109 2 -14.031458181734248 1109 3 -6.015449777708849 1109 4 1.4440256422614723 1109 5 1.078504801984554 1109 6 2.175254439041783 1109 7 3.1084145065477493 1109 8 3.3509013748143133 1109 9 2.834170424805489 1109 10 1.9090723521058237 1109 11 0.34044209665221814 1109 12 1.0038674275360329 1109 13 2.1192590378786775 1109 14 2.7785697177318944 1109 15 2.790142404573679 1109 16 2.7109405031504608 1109 17 3.1229557505506094 1109 18 3.3562541800708487 1109 19 1.8942620382941158 1109 20 0.9471681636492164 1109 21 -0.058029921747468904 1109 22 2.385015998531692 1109 23 2.9350791888095245 1109 24 3.8876016568985428 1109 25 4.05735193253803 1110 1 2.3559489064537438 1110 2 8.386319596043531 1110 3 5.6153001489988625 1110 4 6.838406175829341 1110 5 7.507540275452744 1110 6 3.4783116070277096 1110 7 6.039404831965516 1110 8 6.689023919522608 1110 9 4.639503758138822 1110 10 4.31311105043476 1110 11 2.67470888058655 1110 12 4.942257342516978 1110 13 9.411900491997706 1110 14 6.95995699419041 1110 15 6.066814348285199 1110 16 5.563574921209678 1110 17 6.11431546910822 1110 18 6.221598067493893 1110 19 5.100955676523373 1110 20 5.284641045502045 1110 21 3.3234324060156517 1110 22 5.240978472879391 1110 23 5.867446236596095 1110 24 6.724292363436213 1110 25 7.286415550245447 1111 1 5.038558835531759 1111 2 9.207690754435966 1111 3 7.131460905500042 1111 4 8.036949911053927 1111 5 8.055152568596952 1111 6 7.221517280786575 1111 7 7.0356601511144525 1111 8 7.22120358855334 1111 9 5.710766169486341 1111 10 5.183635873129795 1111 11 7.102434648908062 1111 12 6.343146231405868 1111 13 8.005186544007163 1111 14 5.895143755419724 1111 15 5.567183142611444 1111 16 5.3675276398858 1111 17 6.63936511449829 1111 18 6.238278918557725 1111 19 5.299783099691222 1111 20 4.931354993492587 1111 21 7.666543517126762 1111 22 7.004704241200905 1111 23 6.847512430449527 1111 24 6.807847918991769 1111 25 7.513630680269088 1112 1 -1.7516903629526475 1112 2 7.856384284019178 1112 3 5.814358748694449 1112 4 -0.3893091970778144 1112 5 4.2476962876293065 1112 6 3.3758024746076174 1112 7 5.934521270321682 1112 8 6.076855730785926 1112 9 4.887252616586309 1112 10 3.473081370069462 1112 11 -0.2818862487894016 1112 12 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2204 5 1.5114470854885873 2204 6 4.356834904518749 2204 7 5.280580459205659 2204 8 5.240622485830851 2204 9 3.3807654068843433 2204 10 3.138867322836191 2204 11 4.529812823238881 2204 12 4.6337555504022845 2204 13 2.6178144704923305 2204 14 3.9589964207137065 2204 15 4.172040154736816 2204 16 4.169832985725808 2204 17 -0.6781289753362252 2204 18 2.759834626633676 2204 19 3.5570560349463136 2204 20 3.9900890741758808 2204 21 5.312493337915292 2204 22 4.806992809796952 2204 23 3.742514336954521 2204 24 5.150356879565248 2204 25 5.415075048453332 2205 1 5.6623886227658 2205 2 10.183860967201923 2205 3 7.052311969329832 2205 4 7.678652038713501 2205 5 7.630471717533123 2205 6 6.921091748871682 2205 7 7.0858729170718995 2205 8 6.819501460893768 2205 9 5.6375746801246365 2205 10 4.969167788023412 2205 11 7.275200606354872 2205 12 6.4018696356611855 2205 13 9.533697182305033 2205 14 7.120675670313343 2205 15 5.629310802185913 2205 16 5.4075276398858065 2205 17 6.294684263434462 2205 18 6.165087429196021 2205 19 4.932974589052925 2205 20 5.354333716896843 2205 21 -1.4228181850008994 2205 22 3.2089595603498395 2205 23 4.913044345343142 2205 24 6.56784791899177 2205 25 7.07022642494994 2206 1 3.9015667299327497 2206 2 8.494677208154357 2206 3 6.041119057882039 2206 4 1.484132539725369 2206 5 5.693190089791507 2206 6 6.073842026907744 2206 7 6.084498233850692 2206 8 6.242529774992473 2206 9 4.958420639029995 2206 10 3.8950544951648354 2206 11 3.3758959699895783 2206 12 4.970258485047683 2206 13 7.945690837613245 2206 14 0.4995194049301972 2206 15 2.6572033799904275 2206 16 3.439274435634129 2206 17 4.383710499099649 2206 18 -1.3929143575275305 2206 19 0.8609922375059504 2206 20 2.4886056270501733 2206 21 3.59418704754664 2206 22 -2.2635653717364335 2206 23 -2.9194900673170707 2206 24 1.5112583731477258 2206 25 2.8219265471034873 2207 1 1.5099338862342186 2207 2 -14.031458181734248 2207 3 -6.167608381715562 2207 4 -4.317999755231703 2207 5 1.638924831586003 2207 6 2.3684976266695372 2207 7 3.271890100596366 2207 8 3.449284269994397 2207 9 0.9026279638002883 2207 10 0.5850371047386282 2207 11 -0.9648620803223658 2207 12 -2.148118933238848 2207 13 1.0658384854504313 2207 14 -5.23674163858448 2207 15 -1.1290770439736093 2207 16 -0.041560453332973424 2207 17 1.169550593313879 2207 18 1.1758164383777725 2207 19 0.5469183185414757 2207 20 0.18054707284654725 2207 21 -4.0390717341730635 2207 22 -4.47281779132242 2207 23 -1.2649150189402008 2207 24 0.6592863938617874 2207 25 1.0160577609136539 2208 1 5.094326574762448 2208 2 6.597441654062141 2208 3 6.543041801981893 2208 4 8.779553411735197 2208 5 3.9574143860165094 2208 6 6.542791081576151 2208 7 6.8777430914072255 2208 8 6.866609031728412 2208 9 5.372758273738164 2208 10 4.537833546329669 2208 11 6.858021414795637 2208 12 6.345083834033126 2208 13 9.094221236387416 2208 14 3.125318789483238 2208 15 5.0606862694674675 2208 16 5.4135738265189275 2208 17 6.435445189962074 2208 18 0.9416768852278814 2208 19 3.4572096447773433 2208 20 4.372303880751087 2208 21 7.134720857028238 2208 22 6.087220399535109 2208 23 2.4238622190810446 2208 24 3.8526098237536743 2208 25 5.1126049317451745 2209 1 3.4574632352621926 2209 2 5.458134578446746 2209 3 5.732589238993835 2209 4 0.8978491119781609 2209 5 5.728374865699099 2209 6 6.2432829494108155 2209 7 6.4607260989338435 2209 8 5.36873030464076 2209 9 5.092993954217253 2209 10 4.347186465216055 2209 11 0.1894318569565847 2209 12 4.102060258555188 2209 13 3.0920412662561283 2209 14 4.6559409057037335 2209 15 4.918965177847795 2209 16 4.371486434532958 2209 17 2.033434624462668 2209 18 4.162919334462217 2209 19 4.646069804225736 2209 20 4.69988488662824 2209 21 7.053330847443505 2209 22 5.69894126825395 2209 23 1.4983683079887555 2209 24 -2.5440072846281403 2209 25 1.0668760831765667 2210 1 -2.166345490507318 2210 2 -14.031458181734248 2210 3 -7.720904979206267 2210 4 -2.455550139460307 2210 5 -0.6112821367030001 2210 6 -1.331321821272386 2210 7 -1.0316151110544283 2210 8 -21.26475385825517 2210 9 -13.34056841660002 2210 10 -7.150942130951755 2210 11 -10.791228441350771 2210 12 -4.937423569163933 2210 13 -5.475658862228315 2210 14 -4.183989824423076 2210 15 -6.016323353519166 2210 16 -5.5696900360552775 2210 17 -4.9483094121833275 2210 18 -5.067693480464321 2210 19 -10.502972148737786 2210 20 -7.369452021924356 2210 21 -5.451177280594035 2210 22 -9.57727793737702 2210 23 -7.634350534534008 2210 24 -10.559749421938905 2210 25 -8.01304123987801 2211 1 -0.0864428958367931 2211 2 4.9432253625695495 2211 3 0.6290200605921896 2211 4 1.466028438236414 2211 5 1.1303893813398247 2211 6 -0.1992845352266599 2211 7 -21.088595168034484 2211 8 -13.665296353734373 2211 9 -9.295124331071545 2211 10 -7.379139703031186 2211 11 -10.33688203763728 2211 12 -5.297794094091419 2211 13 -7.254846544369585 2211 14 -5.222720120537339 2211 15 -5.61560397600654 2211 16 -11.884476515398719 2211 17 -7.931393994424031 2211 18 -6.021844970480792 2211 19 -8.855930801274496 2211 20 -6.860293654116198 2211 21 -4.543149068768358 2211 22 -6.224485863065958 2211 23 -8.331775784701875 2211 24 -22.55215208100823 2211 25 -22.334028894198998 2212 1 -0.5827549004171928 2212 2 -14.031458181734248 2212 3 -7.700715945089657 2212 4 -19.69581604639288 2212 5 -20.643570835658366 2212 6 -21.236355059638957 2212 7 -9.850897860699293 2212 8 -9.41981421108711 2212 9 -5.00055566618801 2212 10 -5.180326275470534 2212 11 -5.1440048303388615 2212 12 -2.635461010933966 2212 13 -6.179619161360387 2212 14 -3.5164131698454923 2212 15 -3.4175830519005004 2212 16 -3.644613255825295 2212 17 -3.7271849951443343 2212 18 -2.9217779770743526 2212 19 -3.5582433330711787 2212 20 -3.808370406060938 2212 21 -1.5522494262344253 2212 22 -2.9345857487238263 2212 23 -3.167460603391522 2212 24 -2.1352532880648702 2212 25 -2.5512990891850684 2213 1 -19.102717760212922 2213 2 -14.031458181734248 2213 3 -8.808318299638454 2213 4 -6.105250008657032 2213 5 -20.643570835658366 2213 6 -10.95899656907292 2213 7 -21.088595168034484 2213 8 -10.858150084670264 2213 9 -8.467716367647341 2213 10 -7.248800899250774 2213 11 -6.9335468846126105 2213 12 -5.5604386742545096 2213 13 -19.565877285780072 2213 14 -12.747871098052777 2213 15 -7.288103890708509 2213 16 -6.642404114429734 2213 17 -5.300036732149643 2213 18 -5.343949102317428 2213 19 -5.983581011107653 2213 20 -6.0641849503050835 2213 21 -4.134588558344937 2213 22 -5.284107480999015 2213 23 -4.885337830489856 2213 24 -3.828567175347853 2213 25 -4.720821347029187 2214 1 -19.102717760212922 2214 2 -14.031458181734248 2214 3 -12.841546141380975 2214 4 -19.69581604639288 2214 5 -20.643570835658366 2214 6 -21.236355059638957 2214 7 -17.119479087097318 2214 8 -15.623646297019812 2214 9 -14.825041502777045 2214 10 -14.787446990033825 2214 11 -15.216184978128394 2214 12 -13.34558646294983 2214 13 -19.565877285780072 2214 14 -17.453058270275335 2214 15 -24.31451898504813 2214 16 -19.068584113224112 2214 17 -24.067017864225114 2214 18 -18.599607470312286 2214 19 -15.88928358510247 2214 20 -14.687187140511446 2214 21 -12.17741814421509 2214 22 -13.183350955590612 2214 23 -13.186259123716292 2214 24 -12.000501388782459 2214 25 -11.774923462889094 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 113586.0 3 3.0 4 412.0 5 1900.0 6 2854.0 7 2624.0 8 2321.0 9 2192.0 10 1994.0 11 1819.0 12 1765.0 13 2055.0 14 3115.0 15 6884.0 16 10202.0 17 10890.0 18 11487.0 19 13279.0 20 15346.0 21 17592.0 22 18015.0 23 19546.0 24 20546.0 25 21012.0 26 17489.0 27 13764.0 28 14910.0 29 17050.0 30 16778.0 31 19514.0 32 25248.0 33 31395.0 34 38927.0 35 44528.0 36 44690.0 37 46002.0 38 32718.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.412160626562475 18.024684883131414 12.219541616405309 25.343612873900806 2 13.290035638191148 19.563026093270782 42.68007796451468 24.46686030402339 3 20.751123846072396 23.732805395744627 27.63987511751852 27.87619564066446 4 11.986950355604563 16.436346169690957 37.02722649477506 34.54947697992942 5 12.120601888738488 37.92256277262467 36.86945010147635 13.087385237160484 6 32.53460304581534 36.63572454214019 17.62331032691182 13.206362085132655 7 28.58863772307928 30.412760193209028 21.158687658181975 19.839914425529717 8 24.627000758310842 39.31328022439478 19.5774659371662 16.482253080128178 9 26.81667025779262 15.56323671446631 19.06646797443922 38.55362505330186 10 14.906422018348625 29.938379204892968 34.177675840978594 20.977522935779817 11 33.55964786720884 22.206157509780574 24.049783848491753 20.184410774518835 12 24.5995755540976 24.621872598856914 31.703670671044804 19.07488117600068 13 29.560233740168744 21.0905942405437 24.430412305775036 24.918759713512515 14 20.05046568991467 23.61108346590274 26.627082130766773 29.71136871341582 15 23.87608261573003 31.75351918042612 22.396054915673204 21.974343288170648 16 20.57269242844842 27.78318324106052 30.58167620480523 21.06244812568583 17 21.659271633189782 28.729782682270237 29.290076786218844 20.320868898321137 18 21.607077555354604 26.930544908722855 31.84777502872732 19.61460250719522 19 23.515460133957244 26.76667584182035 26.795424656696333 22.922439367526074 20 23.276147009150716 28.226861725007584 28.21946629115974 20.27752497468196 21 21.29400224658661 26.785166199946207 26.771085481038494 25.149746072428687 22 22.149714914060716 29.37602705215166 26.57233739840511 21.90192063538251 23 21.235936603027614 28.7789046408645 27.95939676747161 22.025761988636276 24 22.73724707183899 28.116325774627533 28.24738911526431 20.899038038269165 25 22.41955024079175 27.275165600834185 27.84700833579449 22.458275822579576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 786.0 1 786.0 2 1621.0 3 2456.0 4 2456.0 5 2456.0 6 3413.0 7 4370.0 8 4370.0 9 4370.0 10 5057.5 11 5745.0 12 5745.0 13 5745.0 14 6630.5 15 7516.0 16 7516.0 17 7516.0 18 10320.5 19 13125.0 20 13125.0 21 13125.0 22 18646.5 23 24168.0 24 24168.0 25 24168.0 26 32574.0 27 40980.0 28 40980.0 29 40980.0 30 50320.5 31 59661.0 32 59661.0 33 59661.0 34 65157.5 35 70654.0 36 70654.0 37 70654.0 38 73787.0 39 76920.0 40 76920.0 41 76920.0 42 79092.0 43 81264.0 44 81264.0 45 81264.0 46 85178.0 47 89092.0 48 89092.0 49 89092.0 50 80193.0 51 71294.0 52 71294.0 53 71294.0 54 59452.0 55 47610.0 56 47610.0 57 47610.0 58 39447.0 59 31284.0 60 31284.0 61 31284.0 62 25281.0 63 19278.0 64 19278.0 65 19278.0 66 14854.5 67 10431.0 68 10431.0 69 10431.0 70 7630.0 71 4829.0 72 4829.0 73 4829.0 74 3378.5 75 1928.0 76 1928.0 77 1928.0 78 1339.0 79 750.0 80 750.0 81 750.0 82 489.0 83 228.0 84 228.0 85 228.0 86 148.0 87 68.0 88 68.0 89 68.0 90 40.0 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.91251738274548 2 36.992890381848504 3 6.192471389957439 4 14.149253821194009 5 14.054137845924162 6 15.416463491719492 7 9.673083984998163 8 7.712671494705411 9 0.11723946951773792 10 1.5730255910133464 11 6.21233738479228 12 6.178173893674788 13 16.914088602336964 14 1.7059170564615656 15 3.0717042013569076 16 3.171034175531114 17 3.0590622046438267 18 1.6393960737570208 19 1.5820555886655467 20 0.2830904263964892 21 4.873489732892669 22 1.729395050357287 23 0.11453047022207774 24 9.399174056214745 25 0.12160396838296822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 664452.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.52702405785948 #Duplication Level Percentage of deduplicated Percentage of total 1 95.98342323612144 83.05159971507588 2 2.758721438097439 4.774079124863796 3 0.5321286490147604 1.3813052524552931 4 0.21943695988599832 0.7594890842895728 5 0.12472006429601391 0.5395828001919488 6 0.07915981525441608 0.4109677943360743 7 0.05706467274259789 0.3456345417876852 8 0.03451036859655881 0.2388863595040034 9 0.024423140459190324 0.19019354958727586 >10 0.1544950037195891 2.5725043386922852 >50 0.014574738429215674 0.8866053289506631 >100 0.014205609899172378 2.4171531506574007 >500 0.0023983497227650784 1.3174458427073328 >1k 7.379537608507934E-4 1.1145531169008358 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 2426 0.3651129050706447 No Hit CCCATGTACTCTGCGTTGATACCAC 2198 0.33079891399228234 No Hit GTCCTACAGTGGACATTTCTAAATT 1260 0.18962995069621283 No Hit GAGTACATGGGAAGCAGTGGTATCA 1098 0.16524895703527118 No Hit CTGTAGGACGTGGAATATGGCAAGA 870 0.13093496595690887 No Hit GCGTTGATACCACTGCTTCCCATGT 777 0.1169384695959979 No Hit CATGTACTCTGCGTTGATACCACTG 771 0.11603546983077785 No Hit GTCCTAAAGTGTGTATTTCTCATTT 742 0.11167097096554754 No Hit GTCCTACAGTGTGCATTTCTCATTT 736 0.11076797120032748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.5049996087001017E-4 0.0 11 0.0 0.0 0.0 1.5049996087001017E-4 0.0 12 0.0 0.0 0.0 3.0099992174002034E-4 0.0 13 0.0 0.0 0.0 3.0099992174002034E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCAAA 35 0.0058668046 19.08701 1 CGTAGCG 30 0.0075150803 18.286566 3 CTGTAGG 645 0.0 17.814543 1 TGTAGGA 705 0.0 17.482014 2 CCTATAC 65 7.4896343E-7 16.879906 3 TGTAAGA 65 3.5891968E-5 16.854452 2 TTGGACT 45 2.684044E-4 16.682686 4 CGCAGAC 50 4.690179E-5 16.338465 7 CAGACTA 75 4.836693E-9 15.9858885 9 TGTAGAA 270 0.0 15.72302 2 CCTACTC 50 5.0319964E-4 15.360714 3 TATACTG 90 1.03809725E-8 15.087753 5 CTAGACT 50 5.9835316E-4 15.014418 4 CTATAGT 100 1.8153514E-9 15.014418 4 CATAGGA 55 0.0038500454 14.939174 2 TGTAGGG 55 0.0038500454 14.939174 2 GATATAC 280 0.0 14.792433 1 GTAGGAC 780 0.0 14.7699175 3 AGTGTTC 40 0.004362953 14.6623535 8 GTCCTAC 675 0.0 14.647512 1 >>END_MODULE