FastQCFastQC Report
Thu 26 May 2016
SRR1926551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664452
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC62960.947547753637584No Hit
GTACATGGGAAGCAGTGGTATCAAC58720.8837357702286999No Hit
GAGTACATGGGAAGCAGTGGTATCA26330.39626639697073684No Hit
GTCCTACAGTGGACATTTCTAAATT25220.3795609013141657No Hit
CTGTAGGACGTGGAATATGGCAAGA25150.37850740158807555No Hit
CATGTACTCTGCGTTGATACCACTG22650.34088241137057307No Hit
GCGTTGATACCACTGCTTCCCATGT18550.27917742741386886No Hit
GTCCTACAGTGTGCATTTCTCATTT17640.26548193097469797No Hit
ACGCAGAGTACATGGGAAGCAGTGG17500.2633749315225178No Hit
CTTTAGGACGTGAAATATGGCGAGG15680.23598393864417594No Hit
GTCCTAAAGTGTGTATTTCTCATTT15370.23131843985720563No Hit
GTATCAACGCAGAGTACATGGGAAG14650.2204824426745649No Hit
CTGTAGGACCTGGAATATGGCGAGA14430.2171714435354247No Hit
GATATACACTGTTCTACAAATCCCG13200.1986599483484134No Hit
ACTCTGCGTTGATACCACTGCTTCC12780.192338949991873No Hit
GTATCAACGCAGAGTACTTTTTTTT12030.18105145292662225No Hit
GCTTCCCATGTACTCTGCGTTGATA11310.1702154557439815No Hit
CTGAAGGACCTGGAATATGGCGAGA9850.14824246145696002No Hit
TATCAACGCAGAGTACATGGGAAGC9840.14809196149609002No Hit
GGTATCAACGCAGAGTACATGGGAA9670.14553346216129984No Hit
GTGTATATCAATGAGTTACAATGAA9540.1435769626699897No Hit
CAGTGGTATCAACGCAGAGTACATG8350.12566746732645848No Hit
GTTCTACAGTGTGGTTTTTATCATT8230.12386146779601838No Hit
GTGTATATCAATGAGTTACAATGAG7910.11904546904817806No Hit
GATATACACTGTTCTACAATGCCGG7890.11874446912643803No Hit
GGTATCAACGCAGAGTACTTTTTTT7410.11152047100467753No Hit
CATGGGAAGCAGTGGTATCAACGCA6820.10264097331334693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA551.1529626E-515.50721919
CCAACGT508.886665E-515.16261719
GCATTAT456.6160073E-414.8183731
TAGGACC5000.014.675744
TTTATAC400.00516154914.2967153
TCCAACG1750.014.07957218
AGGACGA551.9063165E-413.8613835
CCAACGA1257.2759576E-1213.64635419
GATATAC5100.013.4486071
TAGTACC500.001463129913.3415814
TGGCGAA957.602466E-812.96802718
AGGACCT7600.012.9152545
GGACCTG7950.012.82613856
ACTGTTC5000.012.737998
TGTAGGA13500.012.5698422
GTAGGAC13250.012.4444373
CACTGTT5050.012.42363557
GTCCTAC12650.012.2747331
CTGTAGG13400.012.2275281
CAACACC550.002994403512.1287114
Produced by FastQC (version 0.11.5)