FastQCFastQC Report
Thu 26 May 2016
SRR1926550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771807
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14610.18929602867038No Hit
CTGTAGGACGTGGAATATGGCAAGA10920.14148614873925736No Hit
GTCCTAAAGTGTGTATTTCTCATTT8940.1158320668249964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACC358.390052E-418.763943
GTTCTAG602.2775133E-517.7729071
TTCTAGG703.5085923E-617.5714282
TGTAGGA8450.016.173572
CTGTAGG8400.016.0273531
TTAGGGA556.9109476E-515.6150024
TATAGGA1004.0780105E-715.0333342
GTACAGG801.5812093E-514.995891
GATATAC3300.014.9453991
CCTTATA550.003903461214.9090912
TAAGACA754.174126E-614.3137514
TAGGACA1800.014.313754
GTCCTAC9850.014.0739771
TGTAGAA3550.013.8591562
GTAGGAC9650.013.8380013
TCCTACA10550.013.6018962
TAGGACC4050.013.5185424
CTACACT2150.013.4815554
ATTCTAG700.001471621213.329681
GTCTTAC802.6522257E-413.32967951
Produced by FastQC (version 0.11.5)