##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926550_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 771807 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.05523142443642 26.0 2.0 30.0 2.0 31.0 2 15.546838782234419 15.0 2.0 30.0 2.0 31.0 3 18.996867092420775 19.0 10.0 30.0 2.0 31.0 4 20.949829426268483 27.0 2.0 35.0 2.0 35.0 5 21.526962051393678 28.0 2.0 35.0 2.0 37.0 6 21.71832595454563 28.0 2.0 35.0 2.0 36.0 7 21.77811421767359 29.0 2.0 35.0 2.0 37.0 8 22.011088264294052 29.0 2.0 35.0 2.0 37.0 9 24.6136832135495 31.0 10.0 37.0 2.0 39.0 10 25.25305678751294 31.0 10.0 37.0 2.0 39.0 11 22.532096754758637 29.0 2.0 35.0 2.0 39.0 12 23.514811345323377 30.0 2.0 37.0 2.0 39.0 13 20.351971412542255 25.0 2.0 35.0 2.0 39.0 14 23.509598902316252 29.0 2.0 37.0 2.0 40.0 15 24.539395211497176 30.0 2.0 38.0 2.0 40.0 16 24.921621597109123 31.0 2.0 38.0 2.0 40.0 17 24.236747010586843 30.0 2.0 38.0 2.0 40.0 18 24.297889239149164 30.0 2.0 37.0 2.0 40.0 19 24.636459633042975 30.0 2.0 38.0 2.0 40.0 20 24.87101827270289 31.0 2.0 38.0 2.0 40.0 21 22.81436809979697 30.0 2.0 36.0 2.0 40.0 22 23.700609090096357 30.0 2.0 37.0 2.0 40.0 23 23.653673781139585 30.0 2.0 37.0 2.0 40.0 24 22.796653826669104 30.0 2.0 38.0 2.0 40.0 25 22.575319995802058 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.2413163109090455 1101 2 9.484081450152738 1101 3 6.7218293488763585 1101 4 7.794377051968738 1101 5 7.762820025762007 1101 6 6.85117784527035 1101 7 6.649693376428537 1101 8 5.011871389715957 1101 9 5.011989084204778 1101 10 4.794241587309632 1101 11 7.608540622201453 1101 12 5.473242191871627 1101 13 9.786750986400431 1101 14 7.209026784052799 1101 15 6.325424635756189 1101 16 5.778871755134382 1101 17 2.1473337633531315 1101 18 4.33260902392065 1101 19 4.96905802052045 1101 20 3.25469983989705 1101 21 0.4969566398996008 1101 22 3.844652354915297 1101 23 5.013664681883256 1101 24 6.426736903564148 1101 25 7.062481811408816 1102 1 5.038263598639578 1102 2 9.807524657078831 1102 3 6.3487626581607515 1102 4 7.631153892839908 1102 5 7.537643040597196 1102 6 6.07188398225912 1102 7 6.187705731571253 1102 8 6.279745764067762 1102 9 5.566428368147605 1102 10 4.954797568731827 1102 11 7.438154636721002 1102 12 4.866532537725437 1102 13 8.945767084159367 1102 14 6.66022847950304 1102 15 4.6530095209976565 1102 16 5.127994720368633 1102 17 5.806230367943474 1102 18 5.856498639287924 1102 19 4.32864452906534 1102 20 4.691794577688459 1102 21 3.7094132390789305 1102 22 4.892194853900733 1102 23 5.186140670520132 1102 24 6.66778167968355 1102 25 6.491401412797643 1103 1 4.2594098162940845 1103 2 8.2663720288619 1103 3 5.609557749761322 1103 4 6.93408820276105 1103 5 6.4975605443878415 1103 6 5.928790908621764 1103 7 6.101800785208564 1103 8 5.508688657777455 1103 9 4.799369338688567 1103 10 3.9842969538179496 1103 11 4.604378588128029 1103 12 3.6350123478636753 1103 13 7.568443539436604 1103 14 5.974513122114185 1103 15 4.902265600234028 1103 16 4.766015606981242 1103 17 2.575154029965894 1103 18 1.2697764598785604 1103 19 3.3220498334647566 1103 20 3.906357465987817 1103 21 6.195095179706211 1103 22 2.0051776067599114 1103 23 4.095678428050839 1103 24 5.456344508878434 1103 25 1.2746613201854764 1104 1 5.297227371442158 1104 2 9.655373085816064 1104 3 6.699036422015062 1104 4 6.910331006536076 1104 5 7.4040967798216215 1104 6 6.691850581296961 1104 7 6.695519961975872 1104 8 6.633206794801964 1104 9 5.650466783683925 1104 10 4.546174007321014 1104 11 7.769747782879005 1104 12 4.742982201500901 1104 13 8.928388838197165 1104 14 6.75661727733419 1104 15 5.862020250060038 1104 16 5.530808552088029 1104 17 5.910479909455464 1104 18 3.585215493041325 1104 19 4.393809629046732 1104 20 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6.157300512161363 2208 18 1.115116099214692 2208 19 3.754145022425128 2208 20 4.686272868768555 2208 21 7.2640912265194935 2208 22 6.182275163726324 2208 23 2.072206687070011 2208 24 3.2762892499791505 2208 25 4.526441393455325 2209 1 2.4166844217385943 2209 2 3.696156824961564 2209 3 4.305794340671309 2209 4 0.37682271445659765 2209 5 4.329040903423067 2209 6 4.728545308626714 2209 7 5.16404383803226 2209 8 3.8940234566066323 2209 9 3.4131779789234074 2209 10 2.6998478119828277 2209 11 -1.2772004555064917 2209 12 2.1109642026107487 2209 13 1.079646084581924 2209 14 2.613391646353115 2209 15 3.075058478509515 2209 16 2.859496120219948 2209 17 0.32301333110896735 2209 18 2.554509246070932 2209 19 2.9635081706672466 2209 20 3.184539981001553 2209 21 5.187816662896061 2209 22 4.0154563379916866 2209 23 6.495426852737296E-5 2209 24 -3.567072471400966 2209 25 -0.6783246276003254 2210 1 -2.1591849563396153 2210 2 -13.03084392298159 2210 3 -7.38473218308647 2210 4 -2.2707933225736454 2210 5 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-5.703389877686998 2213 5 -18.916284451849933 2213 6 -10.016225955537738 2213 7 -19.245829011631166 2213 8 -10.119666905146197 2213 9 -8.292071738921264 2213 10 -7.196238548306056 2213 11 -6.376615578010128 2213 12 -5.080073637181229 2213 13 -17.74683110315181 2213 14 -11.546893019151506 2213 15 -6.908558947607947 2213 16 -6.215344822099006 2213 17 -4.856558814746972 2213 18 -4.826792941487152 2213 19 -5.727214459946559 2213 20 -5.661847440778988 2213 21 -3.9958145596109595 2213 22 -4.7056828605530505 2213 23 -4.587502199338303 2213 24 -3.7207751093856203 2213 25 -4.503256108725779 2214 1 -17.581071748792446 2214 2 -13.03084392298159 2214 3 -11.65805300729857 2214 4 -18.448906530120816 2214 5 -18.916284451849933 2214 6 -19.162254990550547 2214 7 -15.25687873538807 2214 8 -13.89633069378917 2214 9 -12.949353374771059 2214 10 -13.023418729888904 2214 11 -13.338635101390532 2214 12 -11.39105510616856 2214 13 -17.74683110315181 2214 14 -15.505675945393891 2214 15 -22.094724617975153 2214 16 -16.974083083929965 2214 17 -21.813860266497613 2214 18 -16.461475800575652 2214 19 -13.834722799138163 2214 20 -12.662472585269931 2214 21 -10.252680533350173 2214 22 -11.204245421396514 2214 23 -11.137176416492268 2214 24 -10.146214637075198 2214 25 -9.865813177733868 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 170493.0 3 7.0 4 908.0 5 3213.0 6 4214.0 7 3717.0 8 3266.0 9 3065.0 10 2898.0 11 2732.0 12 2659.0 13 2742.0 14 4047.0 15 7819.0 16 11145.0 17 11961.0 18 12733.0 19 14691.0 20 17823.0 21 20219.0 22 21108.0 23 22650.0 24 23581.0 25 24049.0 26 20014.0 27 16419.0 28 17621.0 29 20097.0 30 19649.0 31 22550.0 32 28154.0 33 34747.0 34 41040.0 35 45474.0 36 43549.0 37 42563.0 38 28190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.251986820310115 17.890651629222553 12.743605996639298 26.113755553828028 2 13.37458256067533 19.46118961792444 42.21742137248542 24.94680644891481 3 20.017610035247543 23.159256807061595 28.94034840946491 27.882784748225948 4 11.638278300185181 15.882253914047281 36.9198071234458 35.55966066232173 5 12.461108703703426 36.90051175323088 36.81639409646991 13.821985446595782 6 32.00263924211694 36.23746474280342 17.3627693146961 14.397126700383545 7 27.583795687239032 30.128410455871418 22.15137562203283 20.136418234856716 8 25.242888783464757 38.857012124087326 19.2744636235273 16.62563546892062 9 26.12945526992655 15.57286998569333 19.394294712238754 38.90338003214137 10 15.325631689169992 29.339870347179104 33.72986013912421 21.604637824526694 11 34.30187751311594 21.25146476795191 24.795481989364376 19.651175729567775 12 24.942374329193104 23.380108982440188 31.57004980676628 20.10746688160043 13 29.39162561957749 20.891355413338122 24.627634123718842 25.089384843365547 14 20.214676121760462 22.886534922414395 26.715689826201395 30.183099129623752 15 22.420898652755604 32.76722796283556 22.711320994168844 22.10055239023999 16 21.383810055955536 27.473552788820655 29.40232109868546 21.74031605653835 17 20.90891606943951 28.2584829406281 29.542376639091376 21.290224350841015 18 20.890153110652456 27.044722210521883 31.978302304031004 20.086822374794657 19 23.672422298963987 25.69537904949844 26.29962177273475 24.33257687880283 20 23.53711637190047 27.961402975338913 27.883179810627173 20.61830084213344 21 21.809950337719762 25.621862240681885 26.09694575621862 26.47124166537973 22 22.595462697108612 28.741522369612166 27.397487709330104 21.265527223949114 23 22.159284000259422 28.014916661262077 28.067578961022114 21.758220377456386 24 23.478434504792332 26.629678229119126 28.538481287083524 21.35340597900502 25 22.7198611505668 27.211453521631014 28.69269574669837 21.375989581103816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 797.0 1 797.0 2 1655.0 3 2513.0 4 2513.0 5 2513.0 6 3681.5 7 4850.0 8 4850.0 9 4850.0 10 5572.5 11 6295.0 12 6295.0 13 6295.0 14 7289.0 15 8283.0 16 8283.0 17 8283.0 18 11648.0 19 15013.0 20 15013.0 21 15013.0 22 21419.5 23 27826.0 24 27826.0 25 27826.0 26 37603.0 27 47380.0 28 47380.0 29 47380.0 30 58843.0 31 70306.0 32 70306.0 33 70306.0 34 76410.0 35 82514.0 36 82514.0 37 82514.0 38 85599.5 39 88685.0 40 88685.0 41 88685.0 42 90518.0 43 92351.0 44 92351.0 45 92351.0 46 91459.0 47 90567.0 48 90567.0 49 90567.0 50 84618.5 51 78670.0 52 78670.0 53 78670.0 54 69120.5 55 59571.0 56 59571.0 57 59571.0 58 50940.5 59 42310.0 60 42310.0 61 42310.0 62 34510.0 63 26710.0 64 26710.0 65 26710.0 66 20862.0 67 15014.0 68 15014.0 69 15014.0 70 11177.5 71 7341.0 72 7341.0 73 7341.0 74 5179.5 75 3018.0 76 3018.0 77 3018.0 78 2114.5 79 1211.0 80 1211.0 81 1211.0 82 820.5 83 430.0 84 430.0 85 430.0 86 269.0 87 108.0 88 108.0 89 108.0 90 70.0 91 32.0 92 32.0 93 32.0 94 21.0 95 10.0 96 10.0 97 10.0 98 6.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.332219065128978 2 36.48787844629551 3 5.676678236916742 4 13.800211710958829 5 13.589407714622956 6 14.972395948728115 7 9.391855735954714 8 7.688580176132116 9 0.10857636688964988 10 1.5059464347952272 11 5.684840899344007 12 5.960298364746627 13 16.32584311881079 14 1.6467847531831146 15 3.0113746053093586 16 3.0724002244084336 17 2.9889596751519485 18 1.5671016199645769 19 1.513590832941396 20 0.28685927958673607 21 4.3561408486836735 22 1.6711431743946348 23 0.11103812222485673 24 9.158636809461433 25 0.11660946324664068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 771807.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.46288619095918 #Duplication Level Percentage of deduplicated Percentage of total 1 96.21326277699815 86.07516177879435 2 2.5845869235173837 4.62449211578554 3 0.5521762681909067 1.4819784791553492 4 0.2094221621568496 0.7494204423561134 5 0.1180230736794665 0.5279342404246651 6 0.061675182150103564 0.33105838809008353 7 0.0490889334013347 0.30741463634834265 8 0.03595003657968131 0.2572955224871079 9 0.02270873955210065 0.18284304438807422 >10 0.13044105024147953 2.2017480846869613 >50 0.01239060238081796 0.7590398833364176 >100 0.009367699590386053 1.7634434685890494 >500 6.043677075747204E-4 0.3930778455306037 >1k 3.021838537873602E-4 0.34509207002738357 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1461 0.18929602867038 No Hit CTGTAGGACGTGGAATATGGCAAGA 1092 0.14148614873925736 No Hit GTCCTAAAGTGTGTATTTCTCATTT 894 0.1158320668249964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.295660702740452E-4 0.0 0.0 0.0 6 0.0 1.295660702740452E-4 0.0 0.0 0.0 7 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 8 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 9 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 10 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 11 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 12 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 13 1.295660702740452E-4 1.295660702740452E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACC 35 8.390052E-4 18.76394 3 GTTCTAG 60 2.2775133E-5 17.772907 1 TTCTAGG 70 3.5085923E-6 17.571428 2 TGTAGGA 845 0.0 16.17357 2 CTGTAGG 840 0.0 16.027353 1 TTAGGGA 55 6.9109476E-5 15.615002 4 TATAGGA 100 4.0780105E-7 15.033334 2 GTACAGG 80 1.5812093E-5 14.99589 1 GATATAC 330 0.0 14.945399 1 CCTTATA 55 0.0039034612 14.909091 2 TAAGACA 75 4.174126E-6 14.313751 4 TAGGACA 180 0.0 14.31375 4 GTCCTAC 985 0.0 14.073977 1 TGTAGAA 355 0.0 13.859156 2 GTAGGAC 965 0.0 13.838001 3 TCCTACA 1055 0.0 13.601896 2 TAGGACC 405 0.0 13.518542 4 CTACACT 215 0.0 13.481555 4 ATTCTAG 70 0.0014716212 13.32968 1 GTCTTAC 80 2.6522257E-4 13.3296795 1 >>END_MODULE