FastQCFastQC Report
Thu 26 May 2016
SRR1926550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771807
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32090.415777519509411No Hit
CTGTAGGACGTGGAATATGGCAAGA31760.41150183919036754No Hit
GTCCTAAAGTGTGTATTTCTCATTT22090.28621144923536584No Hit
CTTTAGGACGTGAAATATGGCGAGG22070.2859523170948178No Hit
GTCCTACAGTGTGCATTTCTCATTT17240.22337190515245392No Hit
GTATCAACGCAGAGTACTTTTTTTT16350.2118405248980639No Hit
CTGTAGGACCTGGAATATGGCGAGA15960.20678744815737612No Hit
GATATACACTGTTCTACAAATCCCG14490.18774123582709148No Hit
CTGAAGGACCTGGAATATGGCGAGA13440.17413679844831675No Hit
GTGTATATCAATGAGTTACAATGAA11320.14666879155021917No Hit
GTGTATATCAATGAGTTACAATGAG10140.13137999525788183No Hit
GGTATCAACGCAGAGTACTTTTTTT9690.1255495220955498No Hit
GTTCTACAGTGTGGTTTTTATCATT9560.12386516318198722No Hit
CTGTAGAACATATTAGATGAGTGAG9090.11777555787910708No Hit
GATATACACTGTTCTACAATGCCGG8910.11544336861417427No Hit
GTACATGGGAAGCAGTGGTATCAAC8230.1066328758355392No Hit
CCCATGTACTCTGCGTTGATACCAC8050.10430068657060639No Hit
GAATATGGCAAGAAAACTGAAAATC7990.10352329014896211No Hit
ACCTGGAATATGGCGAGAAAACTGA7950.10300502586786592No Hit
CTGTAGGACATGGAATATGGCAAGA7800.10106153481375525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC653.229552E-614.6740943
TAGGACC6800.014.0248774
GCCCTAC759.261603E-713.984741
TTACACA1101.7462298E-1013.8718784
GTATTAT551.8978876E-413.8691641
AGGACAT4150.013.3287025
ATAGCGA500.00152975113.26344517
TAGGACA4200.013.1700284
TAGAACT751.4185585E-512.7158884
ATTCCCG905.5324927E-712.64004211
AGGACCT11850.012.5549285
GGACCTG11950.012.4490546
ACAATTC1155.29144E-912.3933818
GTAGGAC16550.012.3909643
CTGTAGG16850.012.279561
TGTAGGA17250.012.22468952
TGTAGAA6050.012.1442312
GGACATG4400.012.1371016
AATGCCG2050.012.01566318
CTAACAC802.7468777E-511.9227013
Produced by FastQC (version 0.11.5)