FastQCFastQC Report
Thu 26 May 2016
SRR1926549_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926549_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579714
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC16690.2879005854611067No Hit
CCCATGTACTCTGCGTTGATACCAC16200.2794481416698579No Hit
GAGTACATGGGAAGCAGTGGTATCA8840.15248898594824345No Hit
CATGTACTCTGCGTTGATACCACTG6470.11160675781506053No Hit
GCGTTGATACCACTGCTTCCCATGT5940.10246431861228122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG405.6728435E-419.88431
TCATATA509.8917066E-519.0042932
GTCTATG602.3855437E-517.6749341
TGTAGGA3400.014.7722452
CTGTAGG3150.014.7291111
CCAACGA709.26193E-814.63448419
ATTATAC450.004624007314.5335973
ATCCCGT903.5197445E-914.32642910
CTAGCCT450.005329933514.2259054
TATAGAC550.001088380713.8729793
AGTACTG755.903332E-613.8407885
GCAATCT400.00722638813.58774213
GTCCTAC3750.013.4329491
TGTACTG704.7411173E-513.3464755
GTCCTAT600.00850105613.25621
GATTGGA600.00850105613.25621
GTATAAT600.00850105613.25621
TAAACAG953.531677E-713.1123265
GTAGGAC4000.013.0802373
TCCAACG1054.783942E-1013.01346918
Produced by FastQC (version 0.11.5)