##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926549_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 579714 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.179364307227356 27.0 7.0 31.0 2.0 31.0 2 16.470112848749555 18.0 2.0 30.0 2.0 31.0 3 20.075342323973544 25.0 15.0 31.0 2.0 31.0 4 22.07041403174669 28.0 2.0 35.0 2.0 37.0 5 22.50727600161459 28.0 2.0 35.0 2.0 37.0 6 23.033452702539527 30.0 2.0 35.0 2.0 36.0 7 23.243802978710193 30.0 7.0 35.0 2.0 37.0 8 23.599136815740174 31.0 10.0 35.0 2.0 37.0 9 26.234453540883955 32.0 17.0 37.0 2.0 39.0 10 26.94512293993245 32.0 23.0 37.0 2.0 39.0 11 24.051903179843855 30.0 7.0 37.0 2.0 39.0 12 25.134309331842942 31.0 11.0 37.0 2.0 39.0 13 21.826359204711288 27.0 2.0 35.0 2.0 39.0 14 25.16001511089951 31.0 17.0 38.0 2.0 40.0 15 26.246575035276017 31.0 17.0 38.0 2.0 40.0 16 26.60998871857502 31.0 17.0 38.0 2.0 40.0 17 25.85279982888114 31.0 11.0 38.0 2.0 40.0 18 25.92072470218073 31.0 16.0 38.0 2.0 40.0 19 26.34088015814695 31.0 17.0 38.0 2.0 40.0 20 26.59810354761141 31.0 17.0 39.0 2.0 40.0 21 24.379747599678463 30.0 2.0 37.0 2.0 40.0 22 25.346044773802255 31.0 2.0 38.0 2.0 40.0 23 25.31890207930117 31.0 2.0 38.0 2.0 40.0 24 24.436282373722214 31.0 2.0 38.0 2.0 40.0 25 24.209039284888757 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.181413026030004 1101 2 9.774880476739385 1101 3 6.415438031507755 1101 4 7.821450716415576 1101 5 7.43936603930144 1101 6 6.926620357556921 1101 7 6.904198342723809 1101 8 5.350611062896419 1101 9 5.1049584329888695 1101 10 4.698436848208601 1101 11 7.418566005048177 1101 12 4.866985457744537 1101 13 9.704469296931933 1101 14 7.004333838127312 1101 15 6.28431445879303 1101 16 5.63390556178334 1101 17 1.5878537396690184 1101 18 4.091222234817163 1101 19 4.588191078523245 1101 20 2.7803299199061335 1101 21 -0.10378959304769708 1101 22 3.450118546719832 1101 23 4.610935280042508 1101 24 6.074657218904246 1101 25 6.76044641588598 1102 1 5.6944786121292985 1102 2 10.637724561493583 1102 3 6.769375292748386 1102 4 8.104235347514816 1102 5 7.907091198037836 1102 6 6.392520562221172 1102 7 6.805040945813602 1102 8 7.051287727056231 1102 9 5.9644154553672415 1102 10 5.281938552496825 1102 11 8.223524155511946 1102 12 5.521631373626409 1102 13 9.865322488684 1102 14 7.390783530756778 1102 15 5.517199551228682 1102 16 5.817086612941921 1102 17 6.797695396139886 1102 18 6.52287017489207 1102 19 5.264944100781882 1102 20 5.60997218775065 1102 21 4.587979312204357 1102 22 5.875078418308917 1102 23 6.303115758739839 1102 24 7.843496993361999 1102 25 7.273034950946428 1103 1 4.708366008050259 1103 2 9.449879829719928 1103 3 6.39797867357289 1103 4 7.765015830605268 1103 5 7.085895611787954 1103 6 6.486139677557958 1103 7 6.68707894729879 1103 8 6.527575506404226 1103 9 5.692446555560238 1103 10 4.988659275012214 1103 11 5.501620195700777 1103 12 4.562813291412471 1103 13 8.520204625303553 1103 14 6.688145596134639 1103 15 5.759590920964573 1103 16 5.414637137442099 1103 17 2.6279125629809457 1103 18 2.0115177563333333 1103 19 3.9858224327164855 1103 20 4.694268937397844 1103 21 6.772171230848397 1103 22 1.9179496635493614 1103 23 4.383872119851581 1103 24 5.856883409512481 1103 25 1.672832586676936 1104 1 5.175436678453217 1104 2 9.957953113429452 1104 3 6.493139333585596 1104 4 7.158729633716419 1104 5 6.835747907182348 1104 6 6.472988874396801 1104 7 6.529852719405863 1104 8 6.436463526866177 1104 9 5.333412312168974 1104 10 4.259582859409424 1104 11 7.326262880201888 1104 12 4.4353761654199815 1104 13 8.721929296510321 1104 14 6.36379832084474 1104 15 5.668877141930665 1104 16 4.858496422644414 1104 17 5.2303602922194194 1104 18 2.880281056693196 1104 19 3.806707215715729 1104 20 3.1440965999238415 1104 21 2.197040982241635 1104 22 4.248972816593557 1104 23 4.603413022451736 1104 24 5.350331622147845 1104 25 4.12518280309428 1105 1 3.5189593945048188 1105 2 9.603863685527445 1105 3 6.625045937892715 1105 4 5.821644254397405 1105 5 2.2616103630833173 1105 6 4.810765475202928 1105 7 5.927066288891577 1105 8 2.988855422518661 1105 9 3.8768398730548164 1105 10 3.563593739415168 1105 11 6.889815758645653 1105 12 4.157321998790717 1105 13 4.564318229785005 1105 14 3.8088076119387537 1105 15 4.2243660689215154 1105 16 4.635110694123895 1105 17 5.323727259013857 1105 18 2.6750232849399183 1105 19 2.888467945358361 1105 20 1.9300100446306132 1105 21 2.6573415289349924 1105 22 4.276582177707411 1105 23 1.4083368534346192 1105 24 4.622496940782998 1105 25 5.524373086495089 1106 1 6.294562938449367 1106 2 10.960473105725017 1106 3 7.809579385233846 1106 4 9.143042547918974 1106 5 8.499579508206754 1106 6 7.5563073457798176 1106 7 7.637639905788987 1106 8 7.301323812643645 1106 9 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5.553811425087538 2204 8 5.697368201369677 2204 9 4.05967261676458 2204 10 4.002479610648937 2204 11 5.395993344762971 2204 12 5.540108454294998 2204 13 3.733432660841558 2204 14 4.924078468790118 2204 15 5.1248426485065295 2204 16 5.044449575744174 2204 17 -0.5201624616982237 2204 18 3.2997704710421196 2204 19 4.165486795993878 2204 20 4.505720072639701 2204 21 5.908400253553236 2204 22 5.597120443411157 2204 23 4.022569059634403 2204 24 5.63378370566253 2204 25 5.761677601484436 2205 1 6.1729189874178125 2205 2 10.336611157228317 2205 3 7.461484520674233 2205 4 7.745675092106421 2205 5 8.011192445405559 2205 6 7.411341283047623 2205 7 7.686385007673422 2205 8 7.309967919833845 2205 9 6.1594801805179245 2205 10 5.68211144550639 2205 11 7.811929064833759 2205 12 7.09573158096444 2205 13 10.178136704077012 2205 14 7.558761684408285 2205 15 6.569328892756307 2205 16 6.4016663121348 2205 17 6.979521211203526 2205 18 6.748276096573065 2205 19 5.356912304497939 2205 20 5.6682386586245705 2205 21 -0.7038883235028202 2205 22 3.9201050249401064 2205 23 5.5787512979856935 2205 24 7.338863136292609 2205 25 7.583268175219764 2206 1 3.8349113501230114 2206 2 8.424182006487614 2206 3 5.867435768857643 2206 4 1.5105119976939037 2206 5 5.455822954086663 2206 6 5.781619268393047 2206 7 6.171503751300797 2206 8 5.993686791406514 2206 9 4.579190923801246 2206 10 3.7370687881148648 2206 11 3.139583705717836 2206 12 4.543256090373077 2206 13 7.769771698011258 2206 14 0.44259757037569614 2206 15 2.366813352061996 2206 16 3.214969218141597 2206 17 4.2247317743957815 2206 18 -1.318593239742075 2206 19 0.9802531819446223 2206 20 2.424584404801891 2206 21 3.2223693124480164 2206 22 -2.27729359991676 2206 23 -2.8900203734206826 2206 24 1.0220014157688588 2206 25 2.401918262756567 2207 1 1.1996565260028653 2207 2 -13.802834879065857 2207 3 -6.218196555693028 2207 4 -4.426956166285393 2207 5 1.3021299756853075 2207 6 1.4300752780367567 2207 7 2.9115749914780835 2207 8 2.8410062211225515 2207 9 0.3100553227462406 2207 10 0.29263703276052055 2207 11 -1.7413563745859761 2207 12 -2.6461312984840895 2207 13 0.33790304822108297 2207 14 -5.257868300503748 2207 15 -1.4566777791703878 2207 16 -0.41988318054961127 2207 17 0.5671269307888167 2207 18 0.54605858068917 2207 19 -0.08670714652153677 2207 20 -0.17011246189921891 2207 21 -4.349384772985815 2207 22 -4.642105846701437 2207 23 -1.4912956269758944 2207 24 0.361456328712638 2207 25 0.8651307778248629 2208 1 4.503299175452231 2208 2 6.352615357900968 2208 3 6.203961559562142 2208 4 7.897759359048305 2208 5 3.601953681525913 2208 6 6.206039744460732 2208 7 6.526150168344625 2208 8 6.491247767016269 2208 9 4.583590593025789 2208 10 4.364958849735132 2208 11 6.414235813522183 2208 12 5.940804617444204 2208 13 8.66890563539511 2208 14 2.9273000947614953 2208 15 4.489655531267601 2208 16 5.235033594869947 2208 17 5.9143256852820265 2208 18 0.418604772048468 2208 19 2.756056779572287 2208 20 4.1779975273399685 2208 21 6.844982972051618 2208 22 5.911503604497135 2208 23 1.7961279598089135 2208 24 3.010342470160083 2208 25 4.545525493579149 2209 1 2.8852142446559768 2209 2 4.6001501955610085 2209 3 5.224027108594232 2209 4 0.7338773473060698 2209 5 4.888228468727345 2209 6 5.6664297376228845 2209 7 5.9547936806409645 2209 8 4.493237816767515 2209 9 4.32113038223142 2209 10 3.4344785498129404 2209 11 -0.9092415862090206 2209 12 3.4296636398676306 2209 13 2.6147074320987933 2209 14 3.9759337039666534 2209 15 4.330314794194674 2209 16 3.606578712881781 2209 17 1.2139507825445293 2209 18 3.575606493300029 2209 19 4.024562591743656 2209 20 3.9991571321594748 2209 21 6.39480731007713 2209 22 5.0105439478042975 2209 23 0.3650746953256423 2209 24 -3.812038515904856 2209 25 -0.1166847820568897 2210 1 -2.2626244351993137 2210 2 -13.802834879065857 2210 3 -7.751772732159038 2210 4 -2.3289855669091075 2210 5 -0.8955422587309592 2210 6 -1.44032328566027 2210 7 -1.3257605834841684 2210 8 -20.90875223298373 2210 9 -13.116383444980531 2210 10 -7.4847609134507636 2210 11 -10.100302393878515 2210 12 -4.78075045507121 2210 13 -5.293976858585271 2210 14 -4.029548017394319 2210 15 -5.94645709287909 2210 16 -5.076245618736177 2210 17 -4.505625343271074 2210 18 -4.585615324570874 2210 19 -9.864798337231232 2210 20 -6.887117189037976 2210 21 -5.09901038982294 2210 22 -9.950915137007948 2210 23 -7.772290038470469 2210 24 -10.287297024650066 2210 25 -8.094101671829295 2211 1 -0.29294475288742916 2211 2 4.578907859523353 2211 3 0.47678510285529185 2211 4 1.2851902846938188 2211 5 0.8016478861629892 2211 6 -0.0824049272271381 2211 7 -20.569584428811773 2211 8 -13.45128335331568 2211 9 -9.139174937642238 2211 10 -7.189067619754162 2211 11 -10.350673431133774 2211 12 -4.936921484304044 2211 13 -7.07992624598382 2211 14 -4.948119267689588 2211 15 -5.442782375558224 2211 16 -11.546052322811123 2211 17 -7.742620097495106 2211 18 -5.818416132161872 2211 19 -8.572818758765209 2211 20 -6.593825052353644 2211 21 -4.443374189066159 2211 22 -5.848543395561862 2211 23 -8.156672378434191 2211 24 -21.859799709934705 2211 25 -21.64404796613649 2212 1 -0.4888519141573475 2212 2 -13.802834879065857 2212 3 -7.495891772749626 2212 4 -19.448212204932737 2212 5 -19.832169541222903 2212 6 -20.28779911809851 2212 7 -9.636559024654728 2212 8 -9.07618872259112 2212 9 -4.809919909037408 2212 10 -5.086382502891205 2212 11 -5.124053209386435 2212 12 -2.647617391465314 2212 13 -5.735659670034879 2212 14 -3.360443302456197 2212 15 -3.4370182863609777 2212 16 -3.6076653095005327 2212 17 -3.6995475456758022 2212 18 -2.5822106565167076 2212 19 -3.1719031166657956 2212 20 -3.2724375503172887 2212 21 -1.2386340282657962 2212 22 -2.4454428617966606 2212 23 -2.4686159737309232 2212 24 -1.5491761533527182 2212 25 -2.1070964649817547 2213 1 -18.63319371554303 2213 2 -13.802834879065857 2213 3 -8.85400713877358 2213 4 -6.067979646793203 2213 5 -19.832169541222903 2213 6 -10.559892141354322 2213 7 -20.569584428811773 2213 8 -10.88782200042559 2213 9 -8.759796379309709 2213 10 -7.687735959038154 2213 11 -6.947821940252755 2213 12 -5.5038663844294895 2213 13 -19.101710478348966 2213 14 -12.569305217704063 2213 15 -7.731781700078148 2213 16 -6.695116300423784 2213 17 -5.479766676671019 2213 18 -5.29573447627072 2213 19 -6.159979812514123 2213 20 -6.099170994648347 2213 21 -4.302300175749551 2213 22 -5.349672412255867 2213 23 -5.071561889193621 2213 24 -3.9981718029579625 2213 25 -5.080094477764398 2214 1 -18.63319371554303 2214 2 -13.802834879065857 2214 3 -12.617150462198422 2214 4 -19.448212204932737 2214 5 -19.832169541222903 2214 6 -20.28779911809851 2214 7 -16.520712248360645 2214 8 -15.346095591379719 2214 9 -14.483014307851995 2214 10 -14.451284364936619 2214 11 -14.916449905514748 2214 12 -12.84591336233938 2214 13 -19.101710478348966 2214 14 -17.12532316956045 2214 15 -23.68759565356652 2214 16 -18.600592199473734 2214 17 -23.368138769694276 2214 18 -18.092418045659308 2214 19 -15.6190052831675 2214 20 -14.389265416750114 2214 21 -11.753503766120827 2214 22 -12.78281631853319 2214 23 -12.705515960418195 2214 24 -11.659298456801872 2214 25 -11.408458993705413 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 105064.0 3 2.0 4 448.0 5 1721.0 6 2425.0 7 2386.0 8 2067.0 9 2010.0 10 1957.0 11 1826.0 12 1795.0 13 1925.0 14 2815.0 15 5876.0 16 8541.0 17 9202.0 18 9556.0 19 10950.0 20 12907.0 21 14735.0 22 15853.0 23 17119.0 24 18146.0 25 18318.0 26 15201.0 27 12547.0 28 13531.0 29 15690.0 30 15355.0 31 17870.0 32 22926.0 33 28337.0 34 33888.0 35 38413.0 36 37117.0 37 36723.0 38 24472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32493119653449 17.79193037188646 12.350644318373755 25.532494113205296 2 13.83909434287825 20.26189429866303 40.163326710037126 25.735684648421596 3 19.900574005740058 24.093964153927253 28.334761026181692 27.670700814151 4 12.485467522030538 16.36112734139907 36.66551669814124 34.48788843842915 5 12.99805960495547 37.245634111149805 34.91298074531071 14.84332553858401 6 32.751193950917816 35.037362690598165 17.437940792952965 14.773502565531052 7 28.272165794291702 30.65188820432566 21.6332530596431 19.442692941739534 8 25.634587264480434 37.67549292626363 19.320660525850105 17.369259283405825 9 26.41706871483877 16.289253658149118 19.324386394482644 37.969291232529464 10 15.452543269011928 29.51256490789469 32.894603234627816 22.140288588465566 11 33.241673745569585 22.918985617622077 23.126409678080787 20.712930958727554 12 24.327173690221688 25.007321287375174 30.19006062025947 20.475444402143673 13 28.40539913763204 20.989280942735235 24.271697214851116 26.333622704781607 14 21.02648513246072 22.644972312352422 26.494072441894506 29.83447011329235 15 24.224759359049095 29.858316864060967 22.594479120754126 23.32244465613581 16 21.965238714683057 28.014029792845236 27.602247187012296 22.418484305459412 17 22.47446749536257 28.12822043595375 27.949297456344212 21.448014612339467 18 22.539050251634794 26.891202329088916 29.537177661346686 21.032569757929608 19 23.466257668711656 27.118875535759308 26.719332156763876 22.695534638765164 20 23.637646961130987 27.458809363993055 27.28736912760966 21.616174547266297 21 21.99490937973861 26.056574481912055 26.674669651238357 25.273846487110983 22 22.877320447994613 27.747193020597372 26.485367239166568 22.890119292241444 23 21.984559091434974 27.662227103266908 27.41218868230788 22.94102512299024 24 22.82939666384799 27.90320684673343 26.97209508677863 22.29530140263995 25 23.023177904490872 26.730571970311836 26.740242381960538 23.506007743236758 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 780.0 1 780.0 2 1468.0 3 2156.0 4 2156.0 5 2156.0 6 2943.0 7 3730.0 8 3730.0 9 3730.0 10 4214.0 11 4698.0 12 4698.0 13 4698.0 14 5513.0 15 6328.0 16 6328.0 17 6328.0 18 8423.0 19 10518.0 20 10518.0 21 10518.0 22 14666.0 23 18814.0 24 18814.0 25 18814.0 26 25018.0 27 31222.0 28 31222.0 29 31222.0 30 38049.0 31 44876.0 32 44876.0 33 44876.0 34 50208.5 35 55541.0 36 55541.0 37 55541.0 38 59565.0 39 63589.0 40 63589.0 41 63589.0 42 67383.5 43 71178.0 44 71178.0 45 71178.0 46 74841.0 47 78504.0 48 78504.0 49 78504.0 50 72359.5 51 66215.0 52 66215.0 53 66215.0 54 56967.5 55 47720.0 56 47720.0 57 47720.0 58 40393.5 59 33067.0 60 33067.0 61 33067.0 62 26930.5 63 20794.0 64 20794.0 65 20794.0 66 16055.5 67 11317.0 68 11317.0 69 11317.0 70 8306.0 71 5295.0 72 5295.0 73 5295.0 74 3754.0 75 2213.0 76 2213.0 77 2213.0 78 1512.0 79 811.0 80 811.0 81 811.0 82 526.0 83 241.0 84 241.0 85 241.0 86 161.5 87 82.0 88 82.0 89 82.0 90 48.0 91 14.0 92 14.0 93 14.0 94 11.5 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.190814781081706 2 36.01793298074568 3 5.757666711516368 4 13.49820773691855 5 13.32363889780133 6 14.541653298005569 7 9.173833994003939 8 7.3948533242253935 9 0.10280931631804649 10 1.4696902265599934 11 5.776986583039223 12 5.754906729870246 13 15.947691447851872 14 1.5944068971941334 15 2.941105441648813 16 3.0647871191656577 17 2.915575611422184 18 1.5260973514526128 19 1.4769351783810638 20 0.2880730843139893 21 4.44253545713921 22 1.612864274452575 23 0.10401680828822488 24 9.01824002870381 25 0.10832927961029058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 579714.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.56753674807996 #Duplication Level Percentage of deduplicated Percentage of total 1 96.58792377247788 87.47730335684635 2 2.4400406966120407 4.419769509144431 3 0.44909438695080134 1.220201171805694 4 0.18196028838275016 0.6591878041918381 5 0.10050787553293491 0.45513753554002645 6 0.050713552180715785 0.2755800900451588 7 0.03718404352369656 0.23573670597922144 8 0.02039196188521757 0.14774798059239103 9 0.019081919637978333 0.155538221223286 >10 0.09312503088048259 1.56626206770407 >50 0.009098216425893353 0.5784137486544163 >100 0.009691536726792932 1.7477746131550793 >500 7.911458552228442E-4 0.4721875453723483 >1k 3.955729276114221E-4 0.5891596497456956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 1669 0.2879005854611067 No Hit CCCATGTACTCTGCGTTGATACCAC 1620 0.2794481416698579 No Hit GAGTACATGGGAAGCAGTGGTATCA 884 0.15248898594824345 No Hit CATGTACTCTGCGTTGATACCACTG 647 0.11160675781506053 No Hit GCGTTGATACCACTGCTTCCCATGT 594 0.10246431861228122 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 1.7249885288262833E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 40 5.6728435E-4 19.8843 1 TCATATA 50 9.8917066E-5 19.004293 2 GTCTATG 60 2.3855437E-5 17.674934 1 TGTAGGA 340 0.0 14.772245 2 CTGTAGG 315 0.0 14.729111 1 CCAACGA 70 9.26193E-8 14.634484 19 ATTATAC 45 0.0046240073 14.533597 3 ATCCCGT 90 3.5197445E-9 14.326429 10 CTAGCCT 45 0.0053299335 14.225905 4 TATAGAC 55 0.0010883807 13.872979 3 AGTACTG 75 5.903332E-6 13.840788 5 GCAATCT 40 0.007226388 13.587742 13 GTCCTAC 375 0.0 13.432949 1 TGTACTG 70 4.7411173E-5 13.346475 5 GTCCTAT 60 0.008501056 13.2562 1 GATTGGA 60 0.008501056 13.2562 1 GTATAAT 60 0.008501056 13.2562 1 TAAACAG 95 3.531677E-7 13.112326 5 GTAGGAC 400 0.0 13.080237 3 TCCAACG 105 4.783942E-10 13.013469 18 >>END_MODULE