FastQCFastQC Report
Thu 26 May 2016
SRR1926549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579714
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44800.7727948609141749No Hit
CCCATGTACTCTGCGTTGATACCAC44710.7712423712382313No Hit
GAGTACATGGGAAGCAGTGGTATCA20960.36155759564198897No Hit
CATGTACTCTGCGTTGATACCACTG16970.29273055334182024No Hit
GTATCAACGCAGAGTACTTTTTTTT14790.2551258034134073No Hit
ACGCAGAGTACATGGGAAGCAGTGG13560.23390844450884402No Hit
GCGTTGATACCACTGCTTCCCATGT13390.23097596400983933No Hit
GTATCAACGCAGAGTACATGGGAAG13070.22545600071759522No Hit
CTGTAGGACGTGGAATATGGCAAGA10910.1881962484949475No Hit
GTCCTACAGTGGACATTTCTAAATT10260.17698382305757668No Hit
GCTTCCCATGTACTCTGCGTTGATA10090.174051342558572No Hit
GGTATCAACGCAGAGTACTTTTTTT9720.16766888500191474No Hit
ACTCTGCGTTGATACCACTGCTTCC9400.16214892170967063No Hit
TATCAACGCAGAGTACTTTTTTTTT8050.13886157657051582No Hit
TATCAACGCAGAGTACATGGGAAGC7890.13610159492439375No Hit
GGTATCAACGCAGAGTACATGGGAA7790.13437660639556748No Hit
CTTTAGGACGTGAAATATGGCGAGG7030.12126669357648771No Hit
CAGTGGTATCAACGCAGAGTACATG6900.11902420848901354No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6330.10919177387470373No Hit
GTCCTAAAGTGTGTATTTCTCATTT6120.10556929796416854No Hit
GTCCTACAGTGTGCATTTCTCATTT6100.10522430025840328No Hit
GTGGTATCAACGCAGAGTACATGGG5970.10298181517092912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG350.00212036516.3399351
GACATAC350.00212036516.3399351
CCAACGA651.9674007E-716.03306219
TCCAACG851.0550139E-915.60436918
CTTATAC551.0864584E-515.6039823
TCTATAC551.0864584E-515.6039823
TCGTCAA400.00513502114.3073772
TTATACC400.00514687114.3024084
GTCGTCA400.005158743414.2974431
ATCCCGT602.608342E-514.22339110
CGTTTCC655.5417113E-513.1281514
TACACTA603.9605037E-412.7132525
CTGTAGG4950.012.7088391
GTAGGAC4750.012.6474383
TCCCGTT751.5047419E-512.64192311
TGTAGGA5400.012.5410342
TAGGACC1750.012.5316344
GAACAGT1457.2759576E-1212.491896
GGACCGC701.0536336E-412.257086
CCTCTAG701.0553431E-412.2549521
Produced by FastQC (version 0.11.5)