FastQCFastQC Report
Thu 26 May 2016
SRR1926548_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926548_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051597
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14420.13712477308322485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA7750.016.1321282
TAGGACC4500.016.1149584
CTGTAGG7800.015.6445181
GTCTAGG851.7028433E-615.60450651
GTAGGAC8900.014.4415893
GTCCTAG1501.2732926E-1014.1480861
CCAACGA1400.014.0054919
GTCCTAC7400.013.9807931
ATAGGAC1201.3005774E-913.6154813
TGCTATA700.00127312213.5895642
TGTAGAA4300.013.5895642
GATTTAT600.00847926413.263831
GCCTTAC905.0326733E-513.2638291
AGGACCT7700.013.0937345
AGGACGT7650.013.0434455
TAGGACG7950.012.7435164
AGAACAG3350.012.7210085
GGACGTG7700.012.5513386
GACGTGG3850.012.4199677
TCCAACG2200.012.407392518
Produced by FastQC (version 0.11.5)