##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926548_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1051597 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.614472083887648 27.0 10.0 31.0 2.0 31.0 2 16.634311432991915 22.0 2.0 30.0 2.0 31.0 3 20.160831573311828 25.0 15.0 31.0 2.0 31.0 4 22.446599790604196 28.0 7.0 35.0 2.0 37.0 5 22.867831498187993 29.0 7.0 35.0 2.0 37.0 6 23.974477865570176 31.0 8.0 35.0 2.0 37.0 7 23.766861259588985 31.0 10.0 35.0 2.0 37.0 8 23.89238843397233 31.0 12.0 35.0 2.0 37.0 9 26.753175408450197 32.0 17.0 37.0 2.0 39.0 10 27.476676901893025 32.0 25.0 37.0 2.0 39.0 11 24.532320841539107 30.0 8.0 37.0 2.0 39.0 12 25.66520634805919 31.0 17.0 37.0 2.0 39.0 13 22.305134000952837 28.0 2.0 37.0 2.0 39.0 14 25.721855425605057 31.0 17.0 38.0 2.0 40.0 15 26.84044267908714 31.0 17.0 38.0 2.0 40.0 16 27.216842573723586 32.0 20.0 39.0 2.0 40.0 17 26.493204145694595 31.0 17.0 38.0 2.0 40.0 18 26.570045369091012 31.0 17.0 38.0 2.0 40.0 19 26.978522190534967 31.0 19.0 39.0 2.0 40.0 20 27.234109644664258 31.0 23.0 39.0 2.0 40.0 21 25.031396057615225 30.0 9.0 38.0 2.0 40.0 22 25.98571506004677 31.0 11.0 38.0 2.0 40.0 23 25.97790788676651 31.0 2.0 38.0 2.0 40.0 24 25.071834552590012 31.0 2.0 39.0 2.0 40.0 25 24.848721515941943 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.7633321660404775 1101 2 10.378179903822172 1101 3 6.955586273781421 1101 4 8.580915775385247 1101 5 8.235380714338323 1101 6 7.8379348211556135 1101 7 7.343887156532212 1101 8 5.7385315606486635 1101 9 5.690875345381222 1101 10 5.383841306118601 1101 11 8.164638021436463 1101 12 5.927042137260926 1101 13 10.317320170654185 1101 14 7.555538104784457 1101 15 6.65929313039485 1101 16 6.239554329414442 1101 17 2.160773433946428 1101 18 4.624050427522985 1101 19 5.313102362017439 1101 20 3.304757383047967 1101 21 0.6792609370954565 1101 22 4.358392946111042 1101 23 5.517992999945417 1101 24 6.7201291233420015 1101 25 7.257059969713758 1102 1 5.931969600815769 1102 2 10.998706517373947 1102 3 7.191457760063194 1102 4 8.557247855656058 1102 5 8.316488459012806 1102 6 7.371347873578511 1102 7 7.304696223468191 1102 8 7.46336277125668 1102 9 6.547579697435687 1102 10 5.926116970522276 1102 11 8.677580100024056 1102 12 5.881018974306691 1102 13 10.460740891071623 1102 14 8.060329432469661 1102 15 6.017571992913318 1102 16 6.485921383221328 1102 17 7.237627545674716 1102 18 7.223472522568709 1102 19 5.845930963637066 1102 20 6.305143120578126 1102 21 5.245601947774542 1102 22 6.388201105979377 1102 23 6.796754992875897 1102 24 8.216752166600713 1102 25 7.992251155260522 1103 1 5.185777723712086 1103 2 9.715376756425375 1103 3 6.40015549627045 1103 4 8.09131662916608 1103 5 7.013192992957922 1103 6 6.965480408267936 1103 7 7.159928667945088 1103 8 6.58085811341655 1103 9 5.9306343455008275 1103 10 5.056729049579541 1103 11 5.958076881344642 1103 12 4.541830463096076 1103 13 8.776690254284226 1103 14 7.068364942759306 1103 15 6.149447165153298 1103 16 5.941494656758433 1103 17 3.49254330413126 1103 18 2.275709227997716 1103 19 4.543899768474155 1103 20 5.063220439546544 1103 21 7.104055427081011 1103 22 2.8249852059621823 1103 23 5.00285058047038 1103 24 6.522330622058568 1103 25 2.224755106475097 1104 1 5.843902457817666 1104 2 10.381300298752912 1104 3 6.941561074842429 1104 4 7.628160101939919 1104 5 7.310284928897712 1104 6 7.214640538809604 1104 7 7.180580348397818 1104 8 7.129454048122874 1104 9 6.084162996427494 1104 10 4.903245225953555 1104 11 8.002200650378398 1104 12 5.256879302011885 1104 13 9.522790992935352 1104 14 7.091240285745261 1104 15 6.35011614837304 1104 16 5.863780677778724 1104 17 6.260335031352856 1104 18 4.0653855292029135 1104 19 4.844641560366984 1104 20 4.123745004621796 1104 21 3.327518378887383 1104 22 5.217238363146116 1104 23 5.594451885887061 1104 24 6.635613208222637 1104 25 5.87537340914789 1105 1 3.6154127363322566 1105 2 9.580927288142831 1105 3 6.284332958131554 1105 4 5.540552343319227 1105 5 1.6259596636457196 1105 6 4.790585499021809 1105 7 5.955389698530443 1105 8 3.1124033186799025 1105 9 4.054620556109192 1105 10 3.618124204733398 1105 11 6.692245411651609 1105 12 3.8765659730994173 1105 13 4.918157372245698 1105 14 3.660653415718734 1105 15 4.114315418930069 1105 16 4.253535319688538 1105 17 5.0070193814854775 1105 18 2.5514100650119325 1105 19 2.485714173099076 1105 20 1.4498308799533604 1105 21 2.5686655937414926 1105 22 3.948712169512163 1105 23 1.5540092466297644 1105 24 4.737826404509107 1105 25 5.360494430368046 1106 1 6.492563135696148 1106 2 11.28047081611178 1106 3 7.866534985049309 1106 4 9.233403493792714 1106 5 8.778176998015272 1106 6 7.987862389823409 1106 7 7.845984937160477 1106 8 7.5265532778001365 1106 9 6.616089577330733 1106 10 5.7808265258956375 1106 11 8.335715149392168 1106 12 5.5956565442481825 1106 13 4.825064087758449 1106 14 6.249310290707502 1106 15 6.230602918256224 1106 16 6.170260760381428 1106 17 6.584017099406093 1106 18 6.6689489457148845 1106 19 6.4895851165682 1106 20 5.858820329828472 1106 21 6.3421209224867106 1106 22 6.782027440127248 1106 23 7.196706873357051 1106 24 7.937241902433676 1106 25 6.97648431599454 1107 1 3.9786720587059534 1107 2 -14.01551667737441 1107 3 -5.621285981869903 1107 4 1.552053810476771 1107 5 4.4397183663503945 1107 6 4.86256506816958 1107 7 4.880126111375816 1107 8 5.362404457777373 1107 9 3.6394013026281122 1107 10 3.0978813035888315 1107 11 5.884881602145157 1107 12 2.858122845957915 1107 13 4.541187872719561 1107 14 3.922019330277312 1107 15 3.6491706624235434 1107 16 3.402114637323592 1107 17 4.19616423823258 1107 18 -0.6756188954021525 1107 19 2.0649737141794873 1107 20 2.362198972466299 1107 21 -0.9151728913500001 1107 22 2.0290903043084008 1107 23 -0.8275713194561121 1107 24 2.657995036498612 1107 25 3.485406719862837 1108 1 5.046755141481924 1108 2 10.46047276853588 1108 3 -0.02075074671786581 1108 4 5.7008377472861085 1108 5 7.3907885577408905 1108 6 7.191175186038457 1108 7 7.288714976030214 1108 8 6.990021085489111 1108 9 5.446628790118837 1108 10 4.707354882105793 1108 11 6.922240123233813 1108 12 2.220205939890427 1108 13 3.3854422530572705 1108 14 4.919912696038995 1108 15 4.965955681417558 1108 16 4.807864998516671 1108 17 0.17061687033999462 1108 18 3.513722980124296 1108 19 4.684723305571101 1108 20 4.9562779161648045 1108 21 5.076955330799084 1108 22 1.8630912637711 1108 23 4.768241629943386 1108 24 5.3005558536038215 1108 25 6.01354380030709 1109 1 3.676408017765354 1109 2 -14.01551667737441 1109 3 -5.558033289106943 1109 4 2.0440279340405816 1109 5 1.752073569610566 1109 6 2.7646135702925783 1109 7 3.9860198613390097 1109 8 4.209136041280999 1109 9 3.624634243555164 1109 10 2.8162869052424746 1109 11 1.1786501158106724 1109 12 1.6131267621686725 1109 13 2.903004228935739 1109 14 3.5341327884975264 1109 15 3.562292618526648 1109 16 3.4061995690401865 1109 17 3.953451040307879 1109 18 3.937588746214505 1109 19 2.4560097138732573 1109 20 1.3093445553927978 1109 21 0.41295168537534366 1109 22 2.8205691597148324 1109 23 3.335964930521804 1109 24 4.413553700157941 1109 25 4.6594981884124245 1110 1 3.0346812157637615 1110 2 8.998984159212316 1110 3 6.225634860164366 1110 4 7.8876904594605435 1110 5 8.383290915929507 1110 6 4.196288592469507 1110 7 6.597301431418877 1110 8 7.4159427656672285 1110 9 5.581414169202258 1110 10 5.064177883311007 1110 11 2.746445954471387 1110 12 5.624510866554807 1110 13 10.22024661537511 1110 14 7.806982170262263 1110 15 6.916236383120307 1110 16 6.6315631386011695 1110 17 7.190924222149366 1110 18 7.039665998046534 1110 19 5.82624493689314 1110 20 6.041102071079774 1110 21 3.9332719652629287 1110 22 6.193479595998305 1110 23 6.569995445352767 1110 24 7.550052650734855 1110 25 7.871880604089608 1111 1 5.03408169952586 1111 2 9.601194912922086 1111 3 7.346274123667385 1111 4 7.969307048682179 1111 5 8.347124211930879 1111 6 7.5221340862511035 1111 7 7.430852392554051 1111 8 7.757311941693196 1111 9 6.143129014144328 1111 10 5.246984635535515 1111 11 7.276365392597793 1111 12 6.639891735252032 1111 13 8.45099993428678 1111 14 6.336521874683321 1111 15 5.69833838807218 1111 16 5.528945441447068 1111 17 6.730980716182014 1111 18 6.429375605489671 1111 19 5.391345214552743 1111 20 5.093044410485938 1111 21 7.957950146209193 1111 22 7.264853597618863 1111 23 7.045667683752171 1111 24 6.8872579850463325 1111 25 7.424969811444356 1112 1 -2.3891157775148493 1112 2 7.6069665441021 1112 3 5.866692365676105 1112 4 -0.4526699528313536 1112 5 3.8036420577674512 1112 6 2.6952492535539356 1112 7 5.693568794804452 1112 8 5.856124631261867 1112 9 4.669529334941252 1112 10 3.3191615321267456 1112 11 -0.48398814732393447 1112 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4.5643263645662735 2208 21 7.144890052608233 2208 22 6.252485058468409 2208 23 2.0834511973839653 2208 24 3.545346886447561 2208 25 4.952044126370616 2209 1 2.9712482846708816 2209 2 4.466005661290787 2209 3 5.2610219095742 2209 4 0.8905904422027717 2209 5 5.265612280284174 2209 6 5.679704671854033 2209 7 6.200994656496853 2209 8 4.858203823178069 2209 9 4.426312312961048 2209 10 3.466470736962318 2209 11 -0.4746987250431438 2209 12 3.158794002941363 2209 13 1.779264355812657 2209 14 3.5997861401039195 2209 15 4.0048791991952015 2209 16 3.676088194519739 2209 17 1.2220088255389605 2209 18 3.4322271285568284 2209 19 4.113767135896499 2209 20 4.041075922937935 2209 21 6.296344759536421 2209 22 4.946517471735568 2209 23 0.4123298407108287 2209 24 -3.0071211842654506 2209 25 0.1260094725918357 2210 1 -2.3849664373734 2210 2 -14.01551667737441 2210 3 -7.764655911986701 2210 4 -2.376947718789488 2210 5 -0.8996952727549541 2210 6 -1.3980380476342589 2210 7 -1.281510020738164 2210 8 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-21.02790771526266 2213 8 -10.918367096249993 2213 9 -8.634710016968846 2213 10 -7.6445424832946 2213 11 -6.867772378620998 2213 12 -5.54715074502019 2213 13 -19.5047952139612 2213 14 -12.682965630042807 2213 15 -7.402784905947893 2213 16 -6.639997491313846 2213 17 -5.127423254985555 2213 18 -5.1393087623067935 2213 19 -6.073959514015026 2213 20 -5.836025853323505 2213 21 -3.931684081937796 2213 22 -4.869848991689718 2213 23 -4.74840971498671 2213 24 -3.788072234124062 2213 25 -4.53868717529809 2214 1 -19.015513987805676 2214 2 -14.01551667737441 2214 3 -12.68003225109914 2214 4 -19.799426104956634 2214 5 -20.134277405319796 2214 6 -21.23204381132302 2214 7 -17.181431790629027 2214 8 -15.551780437967592 2214 9 -14.926894290968315 2214 10 -14.786781632430017 2214 11 -15.12666257918026 2214 12 -13.318821912816313 2214 13 -19.5047952139612 2214 14 -17.445889254589517 2214 15 -24.24829233969062 2214 16 -19.002791833378332 2214 17 -23.941256480583487 2214 18 -18.500347911983518 2214 19 -15.858332482698982 2214 20 -14.624038669701331 2214 21 -12.145944760711426 2214 22 -13.231948203589843 2214 23 -13.166739138118894 2214 24 -11.990902735587627 2214 25 -11.756340955367207 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 175392.0 3 3.0 4 847.0 5 2916.0 6 4012.0 7 4183.0 8 3728.0 9 3406.0 10 3237.0 11 3070.0 12 2951.0 13 3252.0 14 4849.0 15 10561.0 16 15388.0 17 16736.0 18 17495.0 19 20407.0 20 24063.0 21 27350.0 22 28568.0 23 31254.0 24 33036.0 25 33559.0 26 28161.0 27 22328.0 28 24745.0 29 28185.0 30 27416.0 31 31988.0 32 41674.0 33 51636.0 34 63102.0 35 71577.0 36 71001.0 37 72809.0 38 46712.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1239521480964 18.007225812085224 12.457431016416347 25.411391023402025 2 14.558576568777509 19.29670336208576 41.40989541731185 24.73482465182488 3 20.596069992181896 23.00870066332987 28.145074191390414 28.250155153097822 4 11.977932269447516 16.448864135109986 37.018420498235635 34.55478309720686 5 13.817735854437913 37.06613895619952 35.42947594971978 13.686649239642787 6 33.34322716914013 35.971985992996494 17.573675726752263 13.111111111111112 7 28.650718643755933 30.32417377922561 20.96350954079563 20.061598036222826 8 25.19019868340216 37.64663024429198 19.384675229252057 17.778495843053797 9 26.309456378426493 15.520893327177271 19.1874949426677 38.98215535172854 10 15.10558037351784 29.153619539994676 33.70135561415769 22.039444472329794 11 34.309613310893205 21.694800810263335 24.166305541838412 19.82928033700505 12 24.268334244155447 23.641480250620603 31.383938744356094 20.706246760867856 13 28.990523689645464 20.871169430731822 24.765126997028126 25.373179882594588 14 20.72187719001474 22.007008047171407 26.662864599695514 30.60825016311834 15 23.10872127600995 31.03156025901928 22.844763814900563 23.0149546500702 16 22.21486028602429 27.43459284517816 28.016795669351925 22.333751199445633 17 21.882927976021392 27.873857636814215 28.37057135300859 21.872643034155804 18 21.69323207474823 27.054412484824255 30.367434219104496 20.884921221323026 19 23.747778499641374 26.111329386359095 26.331428063932872 23.809464050066655 20 23.600660689184267 27.453218666376756 27.524933196770558 21.42118744766842 21 22.442999852800178 25.907765387354342 26.09266029861672 25.55657446122876 22 22.874977282306492 27.876833366201488 27.127052809453655 22.121136542038368 23 22.646872905623592 27.360171815024675 27.464129044050452 22.528826235301285 24 23.40097710376583 26.615684009196517 27.74905752418995 22.234281362847703 25 23.24403985552088 26.725209778426002 27.70760305180723 22.32314731424589 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 871.0 1 871.0 2 2035.0 3 3199.0 4 3199.0 5 3199.0 6 4635.0 7 6071.0 8 6071.0 9 6071.0 10 7031.0 11 7991.0 12 7991.0 13 7991.0 14 9335.5 15 10680.0 16 10680.0 17 10680.0 18 14761.0 19 18842.0 20 18842.0 21 18842.0 22 26921.5 23 35001.0 24 35001.0 25 35001.0 26 46875.5 27 58750.0 28 58750.0 29 58750.0 30 72875.0 31 87000.0 32 87000.0 33 87000.0 34 96971.0 35 106942.0 36 106942.0 37 106942.0 38 112639.0 39 118336.0 40 118336.0 41 118336.0 42 122403.5 43 126471.0 44 126471.0 45 126471.0 46 127722.0 47 128973.0 48 128973.0 49 128973.0 50 121381.0 51 113789.0 52 113789.0 53 113789.0 54 100723.0 55 87657.0 56 87657.0 57 87657.0 58 75082.0 59 62507.0 60 62507.0 61 62507.0 62 51039.5 63 39572.0 64 39572.0 65 39572.0 66 30878.5 67 22185.0 68 22185.0 69 22185.0 70 16268.5 71 10352.0 72 10352.0 73 10352.0 74 7276.0 75 4200.0 76 4200.0 77 4200.0 78 2860.5 79 1521.0 80 1521.0 81 1521.0 82 1009.5 83 498.0 84 498.0 85 498.0 86 321.5 87 145.0 88 145.0 89 145.0 90 91.0 91 37.0 92 37.0 93 37.0 94 21.5 95 6.0 96 6.0 97 6.0 98 3.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.879173295473457 2 36.069520928644714 3 5.613272004389514 4 13.334100420598386 5 13.3117534568851 6 14.458675709421003 7 9.040820770694477 8 7.593212989386619 9 0.10859673430030706 10 1.4843138578752126 11 5.641609856247213 12 5.688395839851198 13 15.877755451945946 14 1.6234355936732416 15 2.945805284724091 16 2.9796585574131536 17 2.918133087104661 18 1.543176711230633 19 1.4927771760474784 20 0.2848049205161293 21 4.389609327527561 22 1.6318989118455074 23 0.11078388394033076 24 8.965221467919745 25 0.11439743551950035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1051597.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.16057510873627 #Duplication Level Percentage of deduplicated Percentage of total 1 94.59584554235256 84.3421999145334 2 4.070363047188351 7.258318203773234 3 0.6766701571193692 1.809969011030457 4 0.24319490177080608 0.8673358922159078 5 0.1285229216485967 0.5729588804421967 6 0.0750434165026116 0.40145485040983686 7 0.042105929685845575 0.26279322343946016 8 0.029461965718195034 0.21014766458145132 9 0.02227477650469356 0.17874286952193397 >10 0.10010262877145738 1.5822012531370881 >50 0.008211829557838753 0.49498780840344064 >100 0.007303938375688715 1.3319010063405308 >500 7.865767035496367E-4 0.5425182617845428 >1k 1.1236810050709098E-4 0.1444711603865763 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1442 0.13712477308322485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 775 0.0 16.132128 2 TAGGACC 450 0.0 16.114958 4 CTGTAGG 780 0.0 15.644518 1 GTCTAGG 85 1.7028433E-6 15.6045065 1 GTAGGAC 890 0.0 14.441589 3 GTCCTAG 150 1.2732926E-10 14.148086 1 CCAACGA 140 0.0 14.00549 19 GTCCTAC 740 0.0 13.980793 1 ATAGGAC 120 1.3005774E-9 13.615481 3 TGCTATA 70 0.001273122 13.589564 2 TGTAGAA 430 0.0 13.589564 2 GATTTAT 60 0.008479264 13.26383 1 GCCTTAC 90 5.0326733E-5 13.263829 1 AGGACCT 770 0.0 13.093734 5 AGGACGT 765 0.0 13.043445 5 TAGGACG 795 0.0 12.743516 4 AGAACAG 335 0.0 12.721008 5 GGACGTG 770 0.0 12.551338 6 GACGTGG 385 0.0 12.419967 7 TCCAACG 220 0.0 12.4073925 18 >>END_MODULE