FastQCFastQC Report
Thu 26 May 2016
SRR1926548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926548_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051597
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28900.27482010694210807No Hit
CTGTAGGACGTGGAATATGGCAAGA26610.25304370400448084No Hit
CCCATGTACTCTGCGTTGATACCAC20510.19503669181254796No Hit
GTACATGGGAAGCAGTGGTATCAAC19670.187048840953331No Hit
CTTTAGGACGTGAAATATGGCGAGG19320.18372056976199058No Hit
GTCCTAAAGTGTGTATTTCTCATTT17860.16983692422097058No Hit
GTCCTACAGTGTGCATTTCTCATTT17700.16831542881921496No Hit
GTATCAACGCAGAGTACTTTTTTTT16410.15604837214256032No Hit
CTGTAGGACCTGGAATATGGCGAGA14840.14111869851283335No Hit
GATATACACTGTTCTACAAATCCCG14520.13807570770932212No Hit
CTGAAGGACCTGGAATATGGCGAGA11410.10850164083769734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCG400.005270824314.2527549
ACCGAGC400.005270824314.2527548
TGGCGAA759.974865E-713.89427918
ACGGCAA500.001531024613.2627217
TAGGACC5000.012.9713724
TGTAGGA13600.012.3447732
GATATAC5050.012.2687041
GTCCTAT1251.3351382E-912.2007561
GTAGGAC12600.012.1871543
TCCAACG2450.011.98671518
CTGTAGG13750.011.9234671
GACGTGG6200.011.8001057
TTAGAAC1552.3646862E-1111.6914633
GGCGAAA658.210687E-411.65953419
TAAAGCT751.9975717E-411.4453284
GTCCTAC14700.011.2177441
TATACTG2050.011.1661735
ACATTCG855.4730706E-511.14514318
CTACTAG600.005728990311.1204811
ATCCCGT2050.011.10133410
Produced by FastQC (version 0.11.5)