FastQCFastQC Report
Thu 26 May 2016
SRR1926547_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926547_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604158
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC23300.3856607046501081No Hit
CCCATGTACTCTGCGTTGATACCAC23190.3838399888770818No Hit
GAGTACATGGGAAGCAGTGGTATCA10730.1776025476779253No Hit
GTCCTACAGTGGACATTTCTAAATT9330.15442980147577287No Hit
CATGTACTCTGCGTTGATACCACTG8260.13671920259269926No Hit
GCGTTGATACCACTGCTTCCCATGT7940.13142257488935014No Hit
CTGTAGGACGTGGAATATGGCAAGA6920.11453957408492481No Hit
ACGCAGAGTACATGGGAAGCAGTGG6750.11172574061752058No Hit
GTATCAACGCAGAGTACATGGGAAG6690.11073262292314262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATG350.0062055718.9019781
CCTAGAC603.6712663E-718.0679153
TAGGACC2500.016.6139184
TGTAGGA5550.016.5980912
AAGGCTA504.2022904E-516.5487655
CTGTAGG5100.016.3446521
GTGTAGA759.192439E-615.8776621
GCATAGA500.002565435815.8776621
TTTAGGA3050.015.5457052
AGGACGG400.002995175515.5144675
GTATAGA604.8430281E-415.4366151
GTAGGAC6950.014.9742733
TACATGA550.004136317414.7785132
TGTAGAA2450.014.3763782
GTTCAGG658.8862906E-414.2491841
CTAGACA450.005395528414.1999314
TAGACAG756.1308856E-613.7906385
TTAGGAC3250.013.6760223
TAGGACG6450.013.3743534
TAGACTG704.906649E-513.2981165
Produced by FastQC (version 0.11.5)