FastQCFastQC Report
Thu 26 May 2016
SRR1926547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926547_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604158
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC59990.9929521747622312No Hit
GTACATGGGAAGCAGTGGTATCAAC59070.9777243701151024No Hit
GAGTACATGGGAAGCAGTGGTATCA26730.44243393284538146No Hit
CATGTACTCTGCGTTGATACCACTG22610.37423985116476155No Hit
GTCCTACAGTGGACATTTCTAAATT19310.3196183779739737No Hit
GCGTTGATACCACTGCTTCCCATGT18890.31266655411332794No Hit
CTGTAGGACGTGGAATATGGCAAGA18810.3113423971874907No Hit
ACGCAGAGTACATGGGAAGCAGTGG17570.29081796483701283No Hit
GTATCAACGCAGAGTACATGGGAAG16050.2656589832461045No Hit
GTATCAACGCAGAGTACTTTTTTTT15090.24976910013605713No Hit
ACTCTGCGTTGATACCACTGCTTCC13740.22742395201255303No Hit
CTTTAGGACGTGAAATATGGCGAGG12780.21153406890250565No Hit
GTCCTAAAGTGTGTATTTCTCATTT12530.20739607850926411No Hit
GCTTCCCATGTACTCTGCGTTGATA12000.19862353887559214No Hit
GTCCTACAGTGTGCATTTCTCATTT10970.18157501845543714No Hit
TATCAACGCAGAGTACATGGGAAGC10810.1789267046037626No Hit
GGTATCAACGCAGAGTACTTTTTTT10590.17528527305771005No Hit
GGTATCAACGCAGAGTACATGGGAA9860.1632023411094449No Hit
CTGTAGGACCTGGAATATGGCGAGA9700.16055402725777032No Hit
CAGTGGTATCAACGCAGAGTACATG8470.14019511452302214No Hit
CTGAAGGACCTGGAATATGGCGAGA7770.1286087414219459No Hit
GATATACACTGTTCTACAAATCCCG7750.1282777021904866No Hit
TATCAACGCAGAGTACTTTTTTTTT7380.12215347640848917No Hit
CATGGGAAGCAGTGGTATCAACGCA7100.11751892716805867No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7020.11619477024222141No Hit
GTGGTATCAACGCAGAGTACATGGG6720.11122918177033159No Hit
ACATGGGAAGCAGTGGTATCAACGC6650.11007054446022398No Hit
GCAGTGGTATCAACGCAGAGTACAT6630.10973950522876466No Hit
ATACCACTGCTTCCCATGTACTCTG6350.10510495598833418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG350.002116529316.3445281
TAGGACC3750.015.0031424
GTGATCA456.7650265E-414.7745529
CTAGGAC1058.185452E-1114.5345213
AATCCCG2050.013.40495519
ACTGTTC3100.013.1745428
GGACCTG6350.013.0638416
TGTAGGA9150.013.0326062
GATATAC3800.012.7960441
GTCCTAA3700.012.6265151
CTGTAGG9200.012.5396871
GTAGGAC8950.012.4690363
GGCGAGG4050.012.16660919
TCCTATA550.002970033312.141652
GCTATAC550.00297374412.1396273
TCTAGGT550.00297374412.1396273
AGAGTCC550.003069716312.0882698
AGGACCT7050.012.0382225
AAATCCC2600.012.02712518
TAGGACA2650.011.8748894
Produced by FastQC (version 0.11.5)