FastQCFastQC Report
Thu 26 May 2016
SRR1926546_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926546_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668135
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC17810.26656289522327076No Hit
CCCATGTACTCTGCGTTGATACCAC16780.25114684906493445No Hit
GTCCTACAGTGGACATTTCTAAATT10690.15999760527438317No Hit
GAGTACATGGGAAGCAGTGGTATCA8930.1336556234892649No Hit
CTGTAGGACGTGGAATATGGCAAGA7830.11719188487356598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC704.8111397E-616.9979041
TGTAGGA5700.016.3829522
ACAGTCG400.002977032715.5290776
GGACGGG400.002977032715.5290776
CTGTAGG6250.015.4416511
CTGTAAG709.142156E-515.1092471
GTACTAC709.142156E-515.1092471
AGAACAG1350.014.5719675
CCGTTGA459.633964E-414.10029913
GTAGGAC6700.014.0936223
TATAGGA904.1569823E-513.5337452
GACGTGG3300.013.5324017
AAGGACC2050.013.5189164
CTAGGAC652.604986E-413.3584113
GTATATA802.8418537E-413.22059251
AGGACGG550.001605079513.1775215
ACTGTTC2900.013.0829988
TAGGACC3050.012.9307664
GTCCTAC6500.012.8138051
AAACCAG653.8811722E-412.7430965
Produced by FastQC (version 0.11.5)