FastQCFastQC Report
Thu 26 May 2016
SRR1926546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926546_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668135
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC52300.782775936000958No Hit
GTACATGGGAAGCAGTGGTATCAAC48880.7315886759412393No Hit
CTGTAGGACGTGGAATATGGCAAGA23550.3524736767270088No Hit
GAGTACATGGGAAGCAGTGGTATCA23090.34558884057862554No Hit
GTCCTACAGTGGACATTTCTAAATT22940.3433437853128485No Hit
CATGTACTCTGCGTTGATACCACTG19350.28961212928524926No Hit
GTATCAACGCAGAGTACTTTTTTTT15350.2297439888645259No Hit
GCGTTGATACCACTGCTTCCCATGT15210.22764860394980055No Hit
GTCCTAAAGTGTGTATTTCTCATTT15170.22704992254559334No Hit
CTTTAGGACGTGAAATATGGCGAGG15060.22540354868402346No Hit
GTATCAACGCAGAGTACATGGGAAG14240.21313057989777515No Hit
ACGCAGAGTACATGGGAAGCAGTGG14210.21268156884461975No Hit
GTCCTACAGTGTGCATTTCTCATTT13800.20654508445149558No Hit
CTGTAGGACCTGGAATATGGCGAGA11520.17242024441168327No Hit
ACTCTGCGTTGATACCACTGCTTCC11070.1656850786143519No Hit
GATATACACTGTTCTACAAATCCCG9870.1477246364881349No Hit
GGTATCAACGCAGAGTACTTTTTTT9560.1430848556055288No Hit
CTGAAGGACCTGGAATATGGCGAGA9380.14039078928659626No Hit
GCTTCCCATGTACTCTGCGTTGATA9370.14024111893554447No Hit
TATCAACGCAGAGTACATGGGAAGC9150.13694837121240466No Hit
GGTATCAACGCAGAGTACATGGGAA8910.13335628278716127No Hit
TATCAACGCAGAGTACTTTTTTTTT7820.11704221452251415No Hit
CAGTGGTATCAACGCAGAGTACATG7500.11225276328885629No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7440.11135474118254544No Hit
GTGTATATCAATGAGTTACAATGAA7170.10731364170414662No Hit
GTTCTACAGTGTGGTTTTTATCATT6970.10432023468311044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC456.515386E-414.8483623
TATTAAG400.005110718314.3180652
TAGGACC4200.014.0886684
TGTAGGA11150.013.3549652
TGAACAG655.2126525E-513.2146815
GTAGGAC11200.013.2101183
CTGTAGG11000.013.1820171
GGACCTG6900.012.7242886
GTTCTAA603.945544E-412.719491
CTATTAG603.945544E-412.719491
ATAGGAC1154.9367372E-912.450493
ACTGTTC2750.012.4329248
CCAACGA1155.5588316E-912.35172119
CCTACAC1404.7293724E-1112.2726263
AGGACCT7550.012.2617455
GATATAC3450.012.166471
GTTACAA4050.012.16129715
GGCGAGG4750.012.16102819
TAGACCC550.002961125712.1468285
GTCGTCA550.002971181912.1413331
Produced by FastQC (version 0.11.5)