FastQCFastQC Report
Thu 26 May 2016
SRR1926545_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926545_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684599
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34290.5008771558240663No Hit
CCCATGTACTCTGCGTTGATACCAC33440.4884611283393636No Hit
GAGTACATGGGAAGCAGTGGTATCA15520.22670205477951327No Hit
GTCCTACAGTGGACATTTCTAAATT12140.17733008666387184No Hit
CATGTACTCTGCGTTGATACCACTG12120.17703794484070237No Hit
GCGTTGATACCACTGCTTCCCATGT11240.16418370462124543No Hit
ACGCAGAGTACATGGGAAGCAGTGG10280.1501608971091106No Hit
CTGTAGGACGTGGAATATGGCAAGA9470.13832915327074682No Hit
GTATCAACGCAGAGTACATGGGAAG9330.1362841605085605No Hit
GTCCTAAAGTGTGTATTTCTCATTT7640.11159817645073977No Hit
CNCATGTACTCTGCGTTGATACCAC7630.11145210553915504No Hit
ACTCTGCGTTGATACCACTGCTTCC7600.11101389280440083No Hit
GNACATGGGAAGCAGTGGTATCAAC7280.10633962363368922No Hit
GCTTCCCATGTACTCTGCGTTGATA7090.1035642763135792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC501.1546567E-418.6264931
GTACAAG552.394232E-416.9331761
TCTTACA901.2658711E-716.6625232
CTACGCA452.7099822E-416.66194
ACATTAG500.002473336915.9655661
GTATAAT500.002473336915.9655661
TGGACAG855.0913513E-915.9236685
GTAGACC555.9623377E-515.8863483
TACAGCG400.002866599515.6174445
AGACGGA400.002868516115.6158776
GATATAC3250.015.55619051
TCTACGC505.210529E-415.2906093
ATCTACA1107.584276E-814.8723362
GTCCTAC7550.014.626291
GTAATAG550.004664915614.5141511
GTATAGA550.004664915614.5141511
GTCCTAA2350.014.153871
GGACAGT901.7671118E-713.880786
CTGTGCG801.9037725E-713.7971839
CTTACAG951.9035542E-713.7960393
Produced by FastQC (version 0.11.5)