FastQCFastQC Report
Thu 26 May 2016
SRR1926545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926545_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684599
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC98771.4427423937224566No Hit
GTACATGGGAAGCAGTGGTATCAAC89611.3089414387108365No Hit
GAGTACATGGGAAGCAGTGGTATCA40740.5950928937962223No Hit
CATGTACTCTGCGTTGATACCACTG34840.508911055961227No Hit
GCGTTGATACCACTGCTTCCCATGT29090.4249202818000026No Hit
GTCCTACAGTGGACATTTCTAAATT27830.4065153469403257No Hit
CTGTAGGACGTGGAATATGGCAAGA26070.3808068665014118No Hit
GTATCAACGCAGAGTACATGGGAAG25990.3796382992087339No Hit
ACGCAGAGTACATGGGAAGCAGTGG25330.36999761904414114No Hit
ACTCTGCGTTGATACCACTGCTTCC21320.31142318349866127No Hit
CTTTAGGACGTGAAATATGGCGAGG19460.2842539939439No Hit
GTATCAACGCAGAGTACTTTTTTTT18860.27548973924881576No Hit
GTCCTAAAGTGTGTATTTCTCATTT17970.26248942811777404No Hit
GCTTCCCATGTACTCTGCGTTGATA17940.26205121538301984No Hit
TATCAACGCAGAGTACATGGGAAGC15670.2288931184532843No Hit
GGTATCAACGCAGAGTACATGGGAA14850.2169153037033358No Hit
GTCCTACAGTGTGCATTTCTCATTT14510.21194889270945474No Hit
CTGTAGGACCTGGAATATGGCGAGA12770.18653255409371033No Hit
CAGTGGTATCAACGCAGAGTACATG12620.18434149041993927No Hit
GATATACACTGTTCTACAAATCCCG12260.17908293760288868No Hit
CTGAAGGACCTGGAATATGGCGAGA11520.1682736901456181No Hit
GGTATCAACGCAGAGTACTTTTTTT11460.16739726467610966No Hit
CATGGGAAGCAGTGGTATCAACGCA10880.15892515180419486No Hit
GCAGTGGTATCAACGCAGAGTACAT10020.1463630534079074No Hit
ATACCACTGCTTCCCATGTACTCTG9730.14212699697195No Hit
TATCAACGCAGAGTACTTTTTTTTT9610.14037414603293316No Hit
GTGGTATCAACGCAGAGTACATGGG9420.13759879871282313No Hit
GTGTATATCAATGAGTTACAATGAA9240.13496952230429785No Hit
ACATGGGAAGCAGTGGTATCAACGC9180.13409309683478943No Hit
GGGAAGCAGTGGTATCAACGCAGAG8870.12956489857566253No Hit
CTGCTTCCCATGTACTCTGCGTTGA8760.12795811854823044No Hit
CCATGTACTCTGCGTTGATACCACT8220.12007028932265458No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8110.11846350929522245No Hit
CCACTGCTTCCCATGTACTCTGCGT7630.11145210553915504No Hit
GCAGAGTACATGGGAAGCAGTGGTA7580.11072175098123134No Hit
GATATACACTGTTCTACAATGCCGG7530.10999139642330766No Hit
AAGCAGTGGTATCAACGCAGAGTAC7330.10706997819161292No Hit
GTACTCTGCGTTGATACCACTGCTT7310.10677783636844343No Hit
CTTCCCATGTACTCTGCGTTGATAC7240.10575533998735025No Hit
TACCACTGCTTCCCATGTACTCTGC7100.10371034722516392No Hit
GTGTATATCAATGAGTTACAATGAG7060.10312606357882498No Hit
GTCCTTCAGTGTGCATTTCTCATTT7050.10297999266724023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG456.8806246E-414.74204418
GCCTAAG400.00517872714.2896481
TAGGACC3800.014.0420454
TAGGACT903.4868208E-813.76343254
GTCGAGG500.001525630613.2678419
AATGGCG500.001525630613.2678416
CTTATAC655.2744726E-513.1981963
TGAACAG603.984691E-412.7047085
TGTAGGA12950.012.4422252
TAGACCA1001.382723E-712.3870894
GATATAC5700.012.2005181
TAATACC550.002997320112.127224
CTGTAGG12900.012.1111221
GTAGGAC13100.012.0059933
ATACACT5850.011.7274224
CCAACGT658.177753E-411.66403619
ACTGTTC5150.011.6263788
TATACAC5750.011.6042113
CCAACGA907.646697E-611.583034519
GTCCTAA5050.011.5071751
Produced by FastQC (version 0.11.5)