FastQCFastQC Report
Thu 26 May 2016
SRR1926544_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926544_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578784
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15380.26572952949632334No Hit
CTGTAGGACGTGGAATATGGCAAGA11020.1903991817327362No Hit
GTCCTAAAGTGTGTATTTCTCATTT8640.14927848731132856No Hit
GTCCTACAGTGTGCATTTCTCATTT8640.14927848731132856No Hit
CTTTAGGACGTGAAATATGGCGAGG6690.11558716205009123No Hit
CTGTAGGACCTGGAATATGGCGAGA6230.10763946480897883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA654.8425136E-818.1262914
CTAGATA300.00863217317.8516524
GATCTAC450.001212685617.7592451
TAGGACC3800.017.4758264
AGGACTG503.932837E-516.676825
TGTAGGA9100.016.2075332
GTCCTAC9050.016.0421921
CTGTAGG9200.015.7806341
GTATAGA604.6039626E-415.539341
TCCTACA9800.015.3285542
GTCCTAA3050.015.28459551
TGTAGAA3600.015.1737192
TCTTATA550.003920333514.8978332
CCAACGA709.523683E-814.60121119
TTGTAGA1703.6379788E-1214.1029311
AATGGCG350.005704799714.08192916
ACTGTTC3050.013.7608358
TTCAAGG700.001224777813.6563462
TAGGACT952.4114706E-713.5296724
CCTACAG10850.013.39868453
Produced by FastQC (version 0.11.5)