FastQCFastQC Report
Thu 26 May 2016
SRR1926544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926544_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578784
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA31000.5356056836401836No Hit
GTCCTACAGTGGACATTTCTAAATT29810.5150453364294798No Hit
GTCCTACAGTGTGCATTTCTCATTT19760.34140542931387186No Hit
CTTTAGGACGTGAAATATGGCGAGG18900.3265466909935313No Hit
GTCCTAAAGTGTGTATTTCTCATTT17790.3073685519986731No Hit
CTGTAGGACCTGGAATATGGCGAGA17120.2957925581909659No Hit
GTACATGGGAAGCAGTGGTATCAAC13830.23894924531431416No Hit
CCCATGTACTCTGCGTTGATACCAC13540.23393874053187375No Hit
GTATCAACGCAGAGTACTTTTTTTT12910.22305385083208934No Hit
GATATACACTGTTCTACAAATCCCG12420.21458782551003483No Hit
CTGAAGGACCTGGAATATGGCGAGA12090.2088862166196716No Hit
GTGTATATCAATGAGTTACAATGAA8960.15480732017471113No Hit
GGTATCAACGCAGAGTACTTTTTTT8490.146686846906618No Hit
GTGTATATCAATGAGTTACAATGAG8120.14029413390833195No Hit
GTTCTACAGTGTGGTTTTTATCATT7940.13718416542267928No Hit
CTGTAGGACATGGAATATGGCAAGA7450.12871814010062477No Hit
GTCCTTCAGTGTGCATTTCTCATTT7400.12785425996572122No Hit
ACCTGGAATATGGCGAGAAAACTGA7190.12422596339912645No Hit
GATATACACTGTTCTACAATGCCGG7130.12318930723724222No Hit
CTGTAGAACATATTAGATGAGTGAG6740.11645104218499475No Hit
TATCAACGCAGAGTACTTTTTTTTT6540.11299552164538067No Hit
GACCTGGAATATGGCGAGAAAACTG6430.1110949853485929No Hit
GAGTACATGGGAAGCAGTGGTATCA6360.10988555315972798No Hit
CACACACACACACACACACACACAC6220.10746668878199811No Hit
GAATATGGCAAGAAAACTGAAAATC6210.10729391275501741No Hit
TTGTAGAACAGTGTATATCAATGAG6090.10522060043124895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCCG250.0060952218.96333311
GTGTTAG453.440056E-516.9412671
TAGGACC5200.015.2197484
TCCAACG1850.014.33947218
CCAACGT903.7542122E-813.6850919
ACTGTTC5150.013.4714868
GTAAGGC500.001455353513.350533
TTAAGCT500.001456308113.3493694
CAAGACT500.001456308113.3493694
GATCTAC500.001463004913.34124851
TGTAGGA17600.013.3300162
CTGTAGG16950.013.2681611
GATATAC5400.013.2353651
CCAACGA1153.9108272E-1013.18162619
GGACCTG8500.013.125016
GTAGGAC17350.012.9712863
AGGACCT9150.012.9221075
AATCCCG3100.012.8361419
CGAAATC604.1522272E-412.64222313
GGCGAGG5250.012.63239119
Produced by FastQC (version 0.11.5)