##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926544_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578784 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.286740822137446 31.0 28.0 31.0 22.0 34.0 2 28.77662305799746 31.0 28.0 33.0 23.0 34.0 3 29.11370563111627 31.0 28.0 33.0 25.0 34.0 4 32.80837756399624 35.0 33.0 37.0 27.0 37.0 5 32.52946522364129 35.0 33.0 37.0 25.0 37.0 6 32.62092248576325 35.0 33.0 37.0 26.0 37.0 7 32.443355379554376 35.0 33.0 37.0 23.0 37.0 8 32.41166307292531 35.0 33.0 37.0 23.0 37.0 9 33.60252702217062 37.0 33.0 39.0 21.0 39.0 10 33.46820575551501 37.0 33.0 39.0 19.0 39.0 11 33.72405940730912 37.0 34.0 39.0 24.0 39.0 12 33.52041521534804 37.0 33.0 39.0 22.0 39.0 13 33.66209328523249 37.0 34.0 39.0 23.0 39.0 14 34.266550215624484 38.0 33.0 40.0 18.0 41.0 15 34.43777125836236 38.0 34.0 40.0 22.0 41.0 16 34.41094778017361 38.0 34.0 40.0 22.0 41.0 17 34.346687192458674 38.0 34.0 40.0 19.0 41.0 18 34.33080216453806 38.0 34.0 40.0 19.0 41.0 19 34.36999986177918 39.0 34.0 40.0 18.0 41.0 20 34.353682893791124 39.0 34.0 40.0 18.0 41.0 21 34.258379637308565 39.0 34.0 40.0 18.0 41.0 22 34.20646562448167 39.0 34.0 40.0 18.0 41.0 23 33.862986882844034 39.0 34.0 40.0 11.0 41.0 24 33.95108883452203 39.0 34.0 40.0 11.0 41.0 25 33.745590755791454 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6809250334481973 1101 2 0.7091331203584303 1101 3 0.6144008771174363 1101 4 0.6296455870892785 1101 5 0.7096956519651556 1101 6 0.34332011427754594 1101 7 0.5445443658982754 1101 8 0.7132651885375765 1101 9 0.8273071267110197 1101 10 0.9124604061277921 1101 11 0.5364362944950116 1101 12 0.8101776866809587 1101 13 0.7736901845120698 1101 14 1.1977476094211355 1101 15 0.7478137114303891 1101 16 0.6658274300190641 1101 17 0.5885828912944078 1101 18 0.8636663939977609 1101 19 0.8300729137936997 1101 20 0.915697623062826 1101 21 0.7930928404304396 1101 22 0.7032415091807636 1101 23 1.0071504766865687 1101 24 0.9378395405153555 1101 25 0.8240978143761808 1102 1 0.5332331653369735 1102 2 0.6089170632442027 1102 3 0.6908956635653318 1102 4 0.5258735899700397 1102 5 0.7181461467046333 1102 6 0.45547802384668756 1102 7 0.41042260675499165 1102 8 0.6957281613582182 1102 9 0.8831023108292584 1102 10 0.924345504449434 1102 11 0.47096746621345176 1102 12 0.7866208072971901 1102 13 0.714321447037122 1102 14 1.0023353004281503 1102 15 0.6760216898872997 1102 16 0.4847445611563401 1102 17 0.6352582733084375 1102 18 0.6662676903404474 1102 19 0.8679063932526176 1102 20 0.8942660881179378 1102 21 0.7469595582410591 1102 22 0.7682171636146293 1102 23 1.1397985854040016 1102 24 0.7645006752848928 1102 25 0.7590380072619496 1103 1 -0.07679833196052854 1103 2 -0.07978833149713793 1103 3 -0.09471768951997461 1103 4 -0.18696733762235596 1103 5 -0.11513626566272706 1103 6 -0.49630618649978686 1103 7 -0.11134811623562513 1103 8 0.08694188268250969 1103 9 -0.047299113885770794 1103 10 0.02500017386851283 1103 11 -0.14472731351652612 1103 12 -0.09174977539276341 1103 13 -0.3595846399613478 1103 14 0.05668673147479808 1103 15 -0.038063451307827734 1103 16 -0.025957886869100832 1103 17 -0.18733274624320728 1103 18 -0.08707450056091659 1103 19 0.017537651757592698 1103 20 -0.05438547780227054 1103 21 0.02069459099462989 1103 22 -0.0894892220755068 1103 23 0.1546829177597857 1103 24 -0.017248379494674282 1103 25 0.05829650419563848 1104 1 0.2774931138053418 1104 2 0.5299690193732083 1104 3 0.3779505615386185 1104 4 0.02630795623952764 1104 5 0.17854378928042536 1104 6 -0.15556573855496936 1104 7 0.12420781263472946 1104 8 0.14924406015087754 1104 9 0.44307644629230225 1104 10 0.45735437934207823 1104 11 0.1580717239900835 1104 12 0.25608189696421135 1104 13 0.07947836185196877 1104 14 0.30853982770931054 1104 15 0.38343948113482185 1104 16 0.3447404149328577 1104 17 0.26687915053086186 1104 18 0.18291477761844988 1104 19 0.3949012697050307 1104 20 0.4121925814987861 1104 21 0.3671220507136894 1104 22 0.5261142385526867 1104 23 0.595685729149622 1104 24 0.5042688816483576 1104 25 0.6584150865436698 1105 1 0.7858170733104011 1105 2 0.7235893211458304 1105 3 0.8184716817434214 1105 4 0.6908768247861303 1105 5 0.732010489563578 1105 6 0.2839420384901459 1105 7 0.3313846095006383 1105 8 0.36037241048639856 1105 9 1.0326928304511753 1105 10 1.0706558416592884 1105 11 0.7911038018769005 1105 12 0.7514224578423736 1105 13 0.7333358538034105 1105 14 1.218486105976254 1105 15 1.0824901385957446 1105 16 1.0743765811017383 1105 17 1.1275992544833855 1105 18 0.9482351686040573 1105 19 1.1215652858409442 1105 20 1.1959403026888111 1105 21 1.1038011942690247 1105 22 1.2479403895941488 1105 23 1.5796467857416872 1105 24 1.1643712826413406 1105 25 1.390442548628151 1106 1 0.4026009173941567 1106 2 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0.46070568838803894 1107 14 0.8798500197893517 1107 15 0.8785973839110071 1107 16 0.5126433820539447 1107 17 0.6160629058292884 1107 18 0.9015327941915601 1107 19 0.7388362374371127 1107 20 0.5186757601752277 1107 21 0.6122016703723006 1107 22 0.7677627664482074 1107 23 0.7662044980043987 1107 24 1.0006726316006294 1107 25 0.8847506729033157 1108 1 1.2404163177690073 1108 2 0.9206426338459046 1108 3 0.8462526202532743 1108 4 0.6752918870651925 1108 5 -0.625492961955846 1108 6 0.1020621361946965 1108 7 0.5734442183790094 1108 8 0.6990006021936424 1108 9 0.9191416341773007 1108 10 1.040811577250146 1108 11 0.8735761517926974 1108 12 0.7725734447493622 1108 13 0.6366477953020535 1108 14 0.8380433253411752 1108 15 0.8770962860931988 1108 16 0.8207885180489285 1108 17 1.0725702768917103 1108 18 1.1135572598455425 1108 19 0.9437304884565094 1108 20 1.0226133413913416 1108 21 1.0026887430075107 1108 22 1.076820883578641 1108 23 1.3521423480738548 1108 24 1.4361310265461853 1108 25 1.1439813042605707 1109 1 0.3032045395167664 1109 2 -0.25130387914106933 1109 3 0.4499111921199379 1109 4 0.4294796007904793 1109 5 -0.7115009352470558 1109 6 -0.015063660121853673 1109 7 0.3685861811510307 1109 8 0.3993917447813189 1109 9 0.787617046005316 1109 10 -1.3122437902560904 1109 11 -0.033689495357343446 1109 12 0.49706672415593545 1109 13 0.6969884828103687 1109 14 0.7909355238981135 1109 15 0.6519404944634246 1109 16 0.7551853426191641 1109 17 0.517263525053167 1109 18 0.5882718588376008 1109 19 0.5153714936235261 1109 20 0.5257031363886497 1109 21 0.6109096807082182 1109 22 0.7603425555396228 1109 23 0.5233424499097907 1109 24 0.5286686508757157 1109 25 0.7266448927045062 1110 1 0.916360816944092 1110 2 0.2995303226171586 1110 3 0.717429223498339 1110 4 0.8750956480492391 1110 5 1.0900336588953436 1110 6 0.8997361499551673 1110 7 0.9957102251128518 1110 8 1.088912888101234 1110 9 1.4358278851812472 1110 10 0.9864244878783026 1110 11 0.9007983806089754 1110 12 1.0056646444581645 1110 13 1.030107022294409 1110 14 1.4864431465104602 1110 15 1.235950582580287 1110 16 0.9035452014513012 1110 17 1.069593061982296 1110 18 1.4589042982585383 1110 19 1.5448845796047905 1110 20 1.162916324936539 1110 21 1.355719171924278 1110 22 1.2659600827177258 1110 23 1.430509370423735 1110 24 1.4732364219321923 1110 25 1.5256266259778215 1111 1 0.19587247732700064 1111 2 0.22660582644282812 1111 3 0.35074529413166644 1111 4 0.007473689088790536 1111 5 0.3947250159249194 1111 6 -0.019000865236684206 1111 7 0.11239868845715506 1111 8 0.2513300422544589 1111 9 0.44719910107313865 1111 10 0.4126607020674413 1111 11 -0.024811137116863335 1111 12 -0.1952782213784161 1111 13 -0.07726663590010219 1111 14 0.15939524957079243 1111 15 0.013411877524596605 1111 16 -0.010272951789083606 1111 17 0.2933428428400404 1111 18 0.2616803016277913 1111 19 0.24196953425493462 1111 20 -0.007679117307638705 1111 21 0.4710121777096319 1111 22 0.33033551218616 1111 23 0.5935441530849985 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0.8529198881883531 2202 17 0.8608669710188153 2202 18 0.9767784756906721 2202 19 0.9611322149748034 2202 20 0.7077513263108557 2202 21 0.7150216667099656 2202 22 0.7951844840823412 2202 23 0.9989381243243614 2202 24 0.9931114401216519 2202 25 1.0961512100454698 2203 1 -0.23627676726644609 2203 2 -0.16504409879904358 2203 3 -0.31437666645076945 2203 4 -0.6222732553756174 2203 5 -0.4090179105975338 2203 6 -0.5655138093683902 2203 7 -0.4654103027953056 2203 8 -0.43261679334557357 2203 9 -0.07638637567112738 2203 10 -0.08633382813750501 2203 11 -0.18284165654561235 2203 12 -0.23016502112997728 2203 13 -0.18907310535753652 2203 14 0.35458041251792594 2203 15 0.01910806323580516 2203 16 0.06732195264945773 2203 17 -0.0900408706163276 2203 18 -0.0238849318547949 2203 19 -0.11084750370881125 2203 20 -0.12660212775212187 2203 21 -0.03547391452191562 2203 22 -0.08347116791301801 2203 23 -0.07801517652305989 2203 24 0.09107659543010271 2203 25 0.08838677500807535 2204 1 0.07222183944672622 2204 2 0.09510435441142207 2204 3 0.20943027941135028 2204 4 -0.08182954938030207 2204 5 0.08363571268645131 2204 6 -0.22410390743491604 2204 7 -0.053450182985436356 2204 8 0.06389137689625812 2204 9 0.3765415937129859 2204 10 0.19578140864251026 2204 11 -0.022848428579081315 2204 12 -0.27378656288969694 2204 13 -0.10768861823571996 2204 14 0.18392910629307124 2204 15 0.0978083446492306 2204 16 0.06430997264664029 2204 17 -0.13943438974092004 2204 18 -0.1073089034752499 2204 19 0.12224431345022424 2204 20 0.23177132430178915 2204 21 0.16679235435340445 2204 22 0.0840155906945057 2204 23 0.2991378724176741 2204 24 0.3557469558735491 2204 25 0.3688759363094505 2205 1 0.5801254143263179 2205 2 0.49291730941792977 2205 3 0.4522071541087982 2205 4 0.4710807028522339 2205 5 0.14274582590949336 2205 6 0.1520900578955917 2205 7 0.09280079883973258 2205 8 0.3629416670644474 2205 9 0.6967776461448025 2205 10 0.5793176368139754 2205 11 0.2601215902003844 2205 12 0.5662376225014967 2205 13 0.38333509813971034 2205 14 0.8801773569019389 2205 15 0.8127654268814481 2205 16 0.5610353384883524 2205 17 0.6961247279066924 2205 18 0.6946892166944991 2205 19 0.6193228538128963 2205 20 0.6960385389697379 2205 21 0.7093315190532792 2205 22 0.5665508751836441 2205 23 0.9968562708324384 2205 24 1.033951284757201 2205 25 0.9319058158157176 2206 1 0.3123710957082757 2206 2 0.5188290176712904 2206 3 0.5750478450876848 2206 4 0.263787036825363 2206 5 0.5131872846426973 2206 6 0.320036315093283 2206 7 0.6033574207207337 2206 8 0.895571225605714 2206 9 0.8320943448012343 2206 10 0.7376669650289429 2206 11 0.5576263886648789 2206 12 0.7773700601214557 2206 13 0.4274809714602483 2206 14 0.6373750536389835 2206 15 0.6064314537528475 2206 16 0.5523981024038989 2206 17 0.5003473766590929 2206 18 0.39046656794209866 2206 19 0.36884300736375764 2206 20 0.5492055255340347 2206 21 0.7592355497634529 2206 22 0.5809462686577334 2206 23 1.148487748759507 2206 24 0.7623581945460671 2206 25 0.8061860461420167 2207 1 -0.9392986177534866 2207 2 -0.005520930682205716 2207 3 0.1059815340178325 2207 4 0.3105380626219869 2207 5 0.1062613265191672 2207 6 0.34766576900995716 2207 7 0.4860019120454524 2207 8 0.3107520644667119 2207 9 0.6201810272313395 2207 10 0.21881551483691197 2207 11 0.08675007205943075 2207 12 0.24359819120276427 2207 13 0.3927437028470564 2207 14 -5.154326884198785 2207 15 -1.8300519042782355 2207 16 -0.7324271459844027 2207 17 -0.8212771960445409 2207 18 -0.14155807019946565 2207 19 -0.2708508039961899 2207 20 0.1528009973814548 2207 21 0.31281475724163954 2207 22 0.22655802770157862 2207 23 0.6588902871424622 2207 24 0.5062666651436984 2207 25 0.39237008271116736 2208 1 -0.08729467572471705 2208 2 0.23976075625346382 2208 3 0.32697757434096175 2208 4 0.023030039701744442 2208 5 0.2307956256062056 2208 6 0.158468822688981 2208 7 0.38715489044138707 2208 8 0.2310931744208915 2208 9 0.8728916364345451 2208 10 0.4104404685106431 2208 11 0.22400493759482742 2208 12 0.1865715992278183 2208 13 0.03505382087570297 2208 14 0.36222471374400556 2208 15 0.25901992628512005 2208 16 0.13962132499329272 2208 17 0.07902960228316402 2208 18 0.22078440887592166 2208 19 -0.25353987322598215 2208 20 -0.05082559477925486 2208 21 -0.09095432012485816 2208 22 0.09735998534056023 2208 23 0.3619748967896612 2208 24 0.4063588996999883 2208 25 0.32802603016811815 2209 1 0.03493627124849752 2209 2 0.27522264738545843 2209 3 0.6721334143421593 2209 4 0.8789290502399325 2209 5 0.6698804461343215 2209 6 0.7216730766148842 2209 7 0.9371547497195962 2209 8 1.1791715245610987 2209 9 1.1559854143736814 2209 10 0.8349033568910542 2209 11 0.7641083024404551 2209 12 0.9765462036354151 2209 13 0.9722637580856457 2209 14 1.3605050934676797 2209 15 1.2765651190880476 2209 16 1.4468770343108588 2209 17 1.4241704319600714 2209 18 1.2719555425212832 2209 19 1.2541102323622653 2209 20 1.0932707039562217 2209 21 1.1654642352627747 2209 22 1.2188010139980818 2209 23 1.44226136882871 2209 24 1.2824224046294717 2209 25 1.2621804282400433 2210 1 -0.2653640290518027 2210 2 -0.41105614910567567 2210 3 0.2194006006386715 2210 4 0.5498153055549011 2210 5 0.7858477784818376 2210 6 0.6826168312896783 2210 7 0.993993185728911 2210 8 0.7891161506836823 2210 9 1.1950416596988873 2210 10 1.0822568782587325 2210 11 0.4521310066411175 2210 12 0.24908871581391878 2210 13 0.25814417844533466 2210 14 0.6016228543023274 2210 15 0.46016125313918366 2210 16 0.6864300352389847 2210 17 0.4491013284784344 2210 18 0.5462075498369927 2210 19 0.614763099587428 2210 20 0.9273798383686938 2210 21 0.9119994459031417 2210 22 0.960128792737386 2210 23 0.7854579526724095 2210 24 0.4817562177145476 2210 25 0.5580738950131874 2211 1 -1.2532059743405561 2211 2 -0.493445717027587 2211 3 0.1844907564867384 2211 4 0.34175357689424146 2211 5 0.5240142151617135 2211 6 0.26478153461695797 2211 7 0.3483562247508587 2211 8 0.49759328979137507 2211 9 0.6706277162500243 2211 10 0.6628255039621394 2211 11 0.16466083065255788 2211 12 0.5940269062224957 2211 13 0.40578238309363 2211 14 0.5677579390183993 2211 15 0.3402853742976646 2211 16 0.3470811898341637 2211 17 0.37147335798640313 2211 18 0.38571672011224933 2211 19 0.3380239332972437 2211 20 0.34324103903293945 2211 21 0.42583707010368954 2211 22 0.39996393984186085 2211 23 0.505792985551814 2211 24 0.5046811375265037 2211 25 0.6222274053083012 2212 1 0.9753175470930309 2212 2 0.037333000550123785 2212 3 0.5107739718325597 2212 4 0.5648066211015248 2212 5 0.4634375815444969 2212 6 0.40031018017211295 2212 7 0.6413925338295883 2212 8 0.6194392683432213 2212 9 0.8362407495810942 2212 10 0.46620878372363705 2212 11 0.4858620050248277 2212 12 0.5295464882650904 2212 13 0.36780626544923933 2212 14 0.48169017631410327 2212 15 0.4133696552536037 2212 16 -0.23522359667156678 2212 17 0.482679420181519 2212 18 0.24789953763460915 2212 19 0.23825731874577372 2212 20 0.44641618170233954 2212 21 0.4295110296610005 2212 22 0.5905145805998231 2212 23 0.7708327517131934 2212 24 0.6965298027624272 2212 25 0.052203678327195746 2213 1 0.3697556972879923 2213 2 0.2527566237791845 2213 3 -0.27608047799943947 2213 4 -0.731812820479135 2213 5 -1.118444937402007 2213 6 -0.2975342721249632 2213 7 -0.21084844161313043 2213 8 -0.06271003773095174 2213 9 -0.5579885367724486 2213 10 -0.4739699405797353 2213 11 -0.47609198907112216 2213 12 -0.4984327662124244 2213 13 -0.6366575919987625 2213 14 -7.895277485005888 2213 15 -3.3708302808729194 2213 16 -2.0986205727602965 2213 17 -1.1251896464764002 2213 18 -4.990450535164154 2213 19 -2.1719269793077842 2213 20 -4.582904901523833 2213 21 -1.7529570366362677 2213 22 -1.4257682987582356 2213 23 -4.564190921546043 2213 24 -1.897826259085555 2213 25 -1.3724930137310025 2214 1 0.2170037291345963 2214 2 0.01267924756245975 2214 3 -1.8951605799039726 2214 4 -2.138220340964878 2214 5 -0.5370720983497073 2214 6 -0.8222133791103623 2214 7 -0.7846703499392547 2214 8 -0.7964930568969315 2214 9 -6.428591605971601 2214 10 -2.04422892400445 2214 11 -0.8614378986724915 2214 12 -1.1441551022044294 2214 13 -1.3947606971000184 2214 14 -2.585285785572566 2214 15 -1.9894048811388814 2214 16 -1.245418535818466 2214 17 -1.5550972488189458 2214 18 -0.8384697442264226 2214 19 -3.7728966233599515 2214 20 -1.8542788607218057 2214 21 -3.693316459789962 2214 22 -5.914637210120244 2214 23 -6.296858103288244 2214 24 -5.893404555832252 2214 25 -3.5790134104349036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20466.0 3 18.0 4 352.0 5 2341.0 6 3029.0 7 2525.0 8 1781.0 9 1501.0 10 1317.0 11 1178.0 12 1115.0 13 1093.0 14 1074.0 15 1192.0 16 1156.0 17 1173.0 18 1407.0 19 1680.0 20 2154.0 21 2829.0 22 3580.0 23 4423.0 24 5285.0 25 6209.0 26 7143.0 27 7725.0 28 8957.0 29 10737.0 30 13450.0 31 16837.0 32 21460.0 33 27971.0 34 35824.0 35 47663.0 36 64387.0 37 94894.0 38 152858.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.057597363183135 19.845470910798188 9.493958735752836 29.60297299026584 2 11.917078743736745 21.083251378823416 43.61057290955506 23.38909696788478 3 19.614719205794767 24.481369395652543 28.669993138503706 27.233918260048984 4 11.0766603756628 17.87682076169539 38.70159902109215 32.34491984154966 5 11.85065496146269 39.80264749593889 36.03808799640549 12.308609546192928 6 31.392481025655254 39.21289422930166 17.578845860332173 11.81577888471091 7 27.90066297405069 31.44841390081188 21.078038400205003 19.57288472493243 8 23.056449755578914 42.90867153719478 19.131512917173893 14.903365790052417 9 25.738411238258845 16.11343548253027 19.728775872101078 38.419377407109806 10 14.440450680859888 31.376062763530793 34.40623487938066 19.77725167622866 11 34.0885569199456 22.63839286485627 25.413074991922514 17.859975223275608 12 24.23107292993135 25.151660184428458 32.68499432408196 17.932272561558225 13 30.43536103278598 23.082531652568143 25.374578426494164 21.107528888151712 14 18.99385989635657 25.562439372744578 27.076975216528997 28.36672551436985 15 21.67178383160179 35.66643802125539 22.427004808464833 20.234773338677993 16 19.431447488458243 29.67475741575208 32.03891714814917 18.854877947640507 17 19.41954913720266 31.503204280898544 29.622276706804413 19.45496987509438 18 19.188856233034937 29.237874570420015 33.595444076418154 17.97782512012689 19 23.1665028137208 27.678995664952115 27.340865385446055 21.813636135881033 20 22.680065033683093 29.75273723731247 29.20192060154429 18.365277127460146 21 20.685437054237852 28.12292668767623 27.40072289489689 23.79091336318903 22 21.442196052413333 31.724270194062033 27.381026427821087 19.452507325703543 23 20.228617238900867 30.815641068170507 28.738700447835463 20.217041245093164 24 22.995531996503 28.059096516477705 30.118421098099112 18.82695038892018 25 21.099581190910598 29.776220489854595 29.581847459501297 19.54235085973351 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 251.0 1 251.0 2 273.5 3 296.0 4 296.0 5 296.0 6 403.5 7 511.0 8 511.0 9 511.0 10 833.5 11 1156.0 12 1156.0 13 1156.0 14 1758.0 15 2360.0 16 2360.0 17 2360.0 18 4333.0 19 6306.0 20 6306.0 21 6306.0 22 10586.0 23 14866.0 24 14866.0 25 14866.0 26 22859.0 27 30852.0 28 30852.0 29 30852.0 30 43239.5 31 55627.0 32 55627.0 33 55627.0 34 62381.5 35 69136.0 36 69136.0 37 69136.0 38 72283.0 39 75430.0 40 75430.0 41 75430.0 42 77216.0 43 79002.0 44 79002.0 45 79002.0 46 80641.5 47 82281.0 48 82281.0 49 82281.0 50 74388.0 51 66495.0 52 66495.0 53 66495.0 54 54089.0 55 41683.0 56 41683.0 57 41683.0 58 33852.0 59 26021.0 60 26021.0 61 26021.0 62 20292.0 63 14563.0 64 14563.0 65 14563.0 66 10843.5 67 7124.0 68 7124.0 69 7124.0 70 5131.0 71 3138.0 72 3138.0 73 3138.0 74 2143.5 75 1149.0 76 1149.0 77 1149.0 78 767.5 79 386.0 80 386.0 81 386.0 82 249.5 83 113.0 84 113.0 85 113.0 86 74.0 87 35.0 88 35.0 89 35.0 90 18.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08828854978713993 2 0.036455741692928624 3 0.03334577320727595 4 0.031099684856526786 5 0.021424227345607342 6 0.38287167578924086 7 0.21337839332116992 8 0.2240905069939736 9 0.2294465638303754 10 0.017968706805993256 11 0.0025916404047105655 12 0.0039738486205562005 13 0.0 14 0.07861309227622049 15 0.002246088350749157 16 0.00276441643169127 17 0.0046649527284790175 18 0.0036282965665947915 19 0.002246088350749157 20 0.0012094321888649307 21 0.0 22 0.0 23 0.0 24 3.4555205396140875E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 578784.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.51820936737161 #Duplication Level Percentage of deduplicated Percentage of total 1 93.86261418869661 72.7606177844821 2 4.327786426416776 6.709645086004894 3 0.7288052830072657 1.6948704154861123 4 0.29688023143043085 0.920544957482315 5 0.1628442180978304 0.6311696096386772 6 0.11647858029949872 0.5417526584682448 7 0.07651600096556471 0.4151968367961858 8 0.05589526579139677 0.34663207330099033 9 0.04466484248413106 0.31161047499409905 >10 0.2600412907085805 3.832578958027803 >50 0.033848940313384 1.8421227329209149 >100 0.026675620552216943 4.19599038210454 >500 0.004483297571783412 2.3363188258285055 >1k 0.0024658136644808764 3.4609492044645846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 3100 0.5356056836401836 No Hit GTCCTACAGTGGACATTTCTAAATT 2981 0.5150453364294798 No Hit GTCCTACAGTGTGCATTTCTCATTT 1976 0.34140542931387186 No Hit CTTTAGGACGTGAAATATGGCGAGG 1890 0.3265466909935313 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1779 0.3073685519986731 No Hit CTGTAGGACCTGGAATATGGCGAGA 1712 0.2957925581909659 No Hit GTACATGGGAAGCAGTGGTATCAAC 1383 0.23894924531431416 No Hit CCCATGTACTCTGCGTTGATACCAC 1354 0.23393874053187375 No Hit GTATCAACGCAGAGTACTTTTTTTT 1291 0.22305385083208934 No Hit GATATACACTGTTCTACAAATCCCG 1242 0.21458782551003483 No Hit CTGAAGGACCTGGAATATGGCGAGA 1209 0.2088862166196716 No Hit GTGTATATCAATGAGTTACAATGAA 896 0.15480732017471113 No Hit GGTATCAACGCAGAGTACTTTTTTT 849 0.146686846906618 No Hit GTGTATATCAATGAGTTACAATGAG 812 0.14029413390833195 No Hit GTTCTACAGTGTGGTTTTTATCATT 794 0.13718416542267928 No Hit CTGTAGGACATGGAATATGGCAAGA 745 0.12871814010062477 No Hit GTCCTTCAGTGTGCATTTCTCATTT 740 0.12785425996572122 No Hit ACCTGGAATATGGCGAGAAAACTGA 719 0.12422596339912645 No Hit GATATACACTGTTCTACAATGCCGG 713 0.12318930723724222 No Hit CTGTAGAACATATTAGATGAGTGAG 674 0.11645104218499475 No Hit TATCAACGCAGAGTACTTTTTTTTT 654 0.11299552164538067 No Hit GACCTGGAATATGGCGAGAAAACTG 643 0.1110949853485929 No Hit GAGTACATGGGAAGCAGTGGTATCA 636 0.10988555315972798 No Hit CACACACACACACACACACACACAC 622 0.10746668878199811 No Hit GAATATGGCAAGAAAACTGAAAATC 621 0.10729391275501741 No Hit TTGTAGAACAGTGTATATCAATGAG 609 0.10522060043124895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCCG 25 0.00609522 18.963333 11 GTGTTAG 45 3.440056E-5 16.941267 1 TAGGACC 520 0.0 15.219748 4 TCCAACG 185 0.0 14.339472 18 CCAACGT 90 3.7542122E-8 13.68509 19 ACTGTTC 515 0.0 13.471486 8 GTAAGGC 50 0.0014553535 13.35053 3 TTAAGCT 50 0.0014563081 13.349369 4 CAAGACT 50 0.0014563081 13.349369 4 GATCTAC 50 0.0014630049 13.3412485 1 TGTAGGA 1760 0.0 13.330016 2 CTGTAGG 1695 0.0 13.268161 1 GATATAC 540 0.0 13.235365 1 CCAACGA 115 3.9108272E-10 13.181626 19 GGACCTG 850 0.0 13.12501 6 GTAGGAC 1735 0.0 12.971286 3 AGGACCT 915 0.0 12.922107 5 AATCCCG 310 0.0 12.83614 19 CGAAATC 60 4.1522272E-4 12.642223 13 GGCGAGG 525 0.0 12.632391 19 >>END_MODULE