FastQCFastQC Report
Thu 26 May 2016
SRR1926543_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926543_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727395
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12230.16813423243217235No Hit
CTGTAGGACGTGGAATATGGCAAGA9370.1288158428364231No Hit
GTCCTAAAGTGTGTATTTCTCATTT7550.10379504945730998No Hit
GTCCTACAGTGTGCATTTCTCATTT7470.10269523436372259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTAGG450.00121201317.7622451
TAGGACC3250.016.4728154
GTCCTAC7200.016.2820591
TGTAGGA6550.016.275352
CTGTAGG6350.015.3146911
TCCTACA7600.014.9259362
TTAAGCT450.00520830614.276444
GTAGGAC6900.014.1070863
TTAGGAC4400.013.6711923
TTTAGGA4200.013.6671222
CCTACAG9100.013.4608663
GTATTAG600.00823314113.3216841
GTATAGC904.8240065E-513.3216841
GTCTTAG600.00823314113.3216841
CAGGACC1055.6905265E-813.2566934
CTTAGGG500.00908978313.1243453
TGTAGAA2300.013.07292
AGGACCT5300.012.99021155
ATACACT2350.012.75766854
GACAGTG801.3707433E-512.7567417
Produced by FastQC (version 0.11.5)