##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926543_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 727395 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.00568879357158 26.0 2.0 30.0 2.0 31.0 2 15.572444132830167 15.0 2.0 30.0 2.0 31.0 3 18.858921218870076 16.0 7.0 30.0 2.0 31.0 4 20.922042356628793 27.0 2.0 35.0 2.0 35.0 5 21.10419648196647 27.0 2.0 35.0 2.0 35.0 6 22.035658754871836 29.0 2.0 35.0 2.0 36.0 7 21.70417723520233 28.0 2.0 35.0 2.0 36.0 8 21.937185435698623 29.0 2.0 35.0 2.0 37.0 9 24.63599144893765 31.0 11.0 37.0 2.0 39.0 10 25.290058358938403 31.0 10.0 37.0 2.0 39.0 11 22.583509647440525 29.0 2.0 35.0 2.0 39.0 12 23.620034506698563 30.0 2.0 37.0 2.0 39.0 13 20.449831247121576 26.0 2.0 35.0 2.0 39.0 14 23.618815086713546 30.0 2.0 37.0 2.0 40.0 15 24.665870675492684 30.0 2.0 38.0 2.0 40.0 16 24.993996384357878 31.0 2.0 38.0 2.0 40.0 17 24.320950790148405 30.0 2.0 38.0 2.0 40.0 18 24.374319317564733 30.0 2.0 37.0 2.0 40.0 19 24.7233868805807 30.0 2.0 38.0 2.0 40.0 20 24.99156304346332 31.0 2.0 38.0 2.0 40.0 21 22.89544745289698 30.0 2.0 37.0 2.0 40.0 22 23.776982244860083 30.0 2.0 37.0 2.0 40.0 23 23.729738312746168 30.0 2.0 37.0 2.0 40.0 24 22.894766942307825 30.0 2.0 38.0 2.0 40.0 25 22.66301390578709 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.306415802806164 1101 2 9.707127561054499 1101 3 6.498717587926603 1101 4 7.908054419794606 1101 5 7.546035655497029 1101 6 7.332008484173922 1101 7 6.71422602758701 1101 8 5.047270740962269 1101 9 5.104973571686923 1101 10 5.01807039806836 1101 11 7.72438524017107 1101 12 5.201955930738073 1101 13 9.591571141893855 1101 14 7.00772677698172 1101 15 6.3710320307670365 1101 16 5.832372874983811 1101 17 2.0778242265205584 1101 18 4.352268686629131 1101 19 4.915676473548302 1101 20 3.164558380776697 1101 21 -0.006882914661829886 1101 22 4.127793354374841 1101 23 5.216877869867464 1101 24 6.5651682667046565 1101 25 7.112110516716633 1102 1 5.075825102115974 1102 2 9.746763848115307 1102 3 6.211160085707164 1102 4 7.515633735573402 1102 5 7.1551965823428745 1102 6 6.3404164335777295 1102 7 5.836607264341577 1102 8 6.059521666022324 1102 9 5.276921129534777 1102 10 4.853207766526133 1102 11 7.514822733890391 1102 12 4.8178759886515365 1102 13 9.138322118479483 1102 14 6.6498634304821636 1102 15 4.94732988314275 1102 16 5.31178600830831 1102 17 6.239838549989784 1102 18 6.026534255193027 1102 19 5.086689530320523 1102 20 5.481758769170707 1102 21 4.1719236250535054 1102 22 5.418300295605604 1102 23 6.015963338951799 1102 24 7.45637313942369 1102 25 7.214874911713597 1103 1 4.276709066588317 1103 2 8.310105608964083 1103 3 5.274401456089862 1103 4 7.059495122758296 1103 5 5.996034103015386 1103 6 5.82024455453239 1103 7 5.5451724203959145 1103 8 5.087084132668913 1103 9 4.234506818081282 1103 10 3.617823165366886 1103 11 4.181212666311755 1103 12 2.970207293040712 1103 13 6.884460387853519 1103 14 5.3153122022840655 1103 15 4.380280940148683 1103 16 4.114685839758003 1103 17 2.139603292858098 1103 18 1.0811083618499708 1103 19 3.310132949725471 1103 20 3.638059459751439 1103 21 5.7253683389670975 1103 22 1.6637701758239274 1103 23 3.7542169939573213 1103 24 4.749915522693357 1103 25 0.7670772936094963 1104 1 5.080021091502779 1104 2 9.561347746362408 1104 3 6.416315013571371 1104 4 7.189568677781139 1104 5 6.809933748558034 1104 6 6.517588173275602 1104 7 6.409607849740066 1104 8 6.418291746330887 1104 9 5.414652596350795 1104 10 4.496885082714808 1104 11 7.416873022408218 1104 12 4.499411607995533 1104 13 8.452726722600584 1104 14 6.437850977459757 1104 15 5.619903330379593 1104 16 5.187767057616096 1104 17 5.613713051704167 1104 18 3.489389960800942 1104 19 4.040071790465529 1104 20 3.6001473774681614 1104 21 2.66467520068775 1104 22 4.744032740619961 1104 23 5.055252193826192 1104 24 5.9447057167229005 1104 25 5.247251756289032 1105 1 2.9862659355879124 1105 2 8.870551398125045 1105 3 5.837958856721567 1105 4 5.448423102243549 1105 5 2.230945132818057 1105 6 4.44051068331245 1105 7 5.491016308190268 1105 8 3.1162926262736903 1105 9 3.7960940526561338 1105 10 3.456930179783498 1105 11 6.283547938538668 1105 12 3.7506627766695715 1105 13 4.79433701793139 1105 14 3.4012646055739317 1105 15 3.9410824537365734 1105 16 3.9693900021247046 1105 17 4.664062279004391 1105 18 2.4012994366850116 1105 19 2.3837444517425475 1105 20 1.4626013179620294 1105 21 1.9898553139803887 1105 22 3.6463865876199044 1105 23 1.1576280393962293 1105 24 4.2537581283161465 1105 25 5.0189340969368885 1106 1 7.081413107829228 1106 2 11.444564322026775 1106 3 8.224841021587423 1106 4 9.797435838950875 1106 5 9.324235336560445 1106 6 8.260201288399646 1106 7 8.151243487320336 1106 8 7.8128504955520945 1106 9 7.237648215485315 1106 10 6.453216242785647 1106 11 8.794038313002357 1106 12 6.247237119055626 1106 13 4.979588345139721 1106 14 6.465086666060188 1106 15 6.480782643177314 1106 16 6.861038136545261 1106 17 7.164492826136197 1106 18 7.195924710924814 1106 19 7.2005522629906835 1106 20 6.545693170512738 1106 21 6.673725146478834 1106 22 7.232754342260453 1106 23 7.496610899876497 1106 24 8.107563041794894 1106 25 7.230841907436247 1107 1 3.317145864074032 1107 2 -13.063260404107812 1107 3 -5.334493888673872 1107 4 1.1840659822404227 1107 5 3.697499936924242 1107 6 4.179834281183641 1107 7 4.018455608096723 1107 8 4.682986403735583 1107 9 2.93059901197773 1107 10 2.4286372561895355 1107 11 4.969904125539653 1107 12 2.466345028379873 1107 13 3.6892790228113306 1107 14 3.2319613530112257 1107 15 3.0694105480919127 1107 16 2.9600538746697715 1107 17 3.3675019306184595 1107 18 -1.6711987376105384 1107 19 1.1001105793684474 1107 20 1.2235109706263358 1107 21 -2.234645273420174 1107 22 0.7385144431071531 1107 23 -2.4318485757110757 1107 24 1.1399391942344579 1107 25 1.8302710499277453 1108 1 5.352596447181583 1108 2 10.365970365122958 1108 3 0.7905879819822701 1108 4 6.009761588318877 1108 5 7.4124946359465085 1108 6 7.338137968308157 1108 7 7.470934109687015 1108 8 7.125178652528138 1108 9 5.933468607925427 1108 10 5.024034829519728 1108 11 7.103955250524926 1108 12 2.7876054830859616 1108 13 3.4788419866468843 1108 14 4.9968406085183545 1108 15 4.972553438891769 1108 16 5.01644803181431 1108 17 -0.011088009303790614 1108 18 3.558172213031469 1108 19 4.603247580193042 1108 20 4.92083220987713 1108 21 4.906821330420087 1108 22 2.0971361888958207 1108 23 4.791926898431932 1108 24 5.646690283879881 1108 25 6.066742954745745 1109 1 3.801717139096141 1109 2 -13.063260404107812 1109 3 -4.842720889725239 1109 4 2.435253660131224 1109 5 1.1522259948771847 1109 6 2.9366361992084293 1109 7 4.270487248200972 1109 8 4.687840164780791 1109 9 4.1870474311465 1109 10 3.309702500860311 1109 11 1.5941478858282956 1109 12 2.247477059550384 1109 13 3.179484104324775 1109 14 4.140433846626074 1109 15 4.253300392108912 1109 16 4.0008314677991095 1109 17 4.541545983619809 1109 18 4.731705170704121 1109 19 3.1032777204105777 1109 20 2.469156151693408 1109 21 1.5583230995198143 1109 22 3.638499050906045 1109 23 4.1839764332242275 1109 24 5.278849895988387 1109 25 5.478564472240176 1110 1 3.4538040567762884 1110 2 9.01614406239343 1110 3 6.257568957995506 1110 4 7.896254557715075 1110 5 8.472635248022602 1110 6 4.1520006647514975 1110 7 6.564987045998016 1110 8 7.619909835323838 1110 9 5.909763066155367 1110 10 5.8021737873361054 1110 11 3.8642861024686823 1110 12 6.266861066212513 1110 13 10.772390373743658 1110 14 8.422101154424062 1110 15 7.619220105558437 1110 16 7.267374417256825 1110 17 7.760913644955927 1110 18 7.752522916091852 1110 19 6.467159904427948 1110 20 6.705067114757195 1110 21 4.589029767144655 1110 22 6.728203186414433 1110 23 7.103730041525399 1110 24 7.986136106874092 1110 25 8.46690010941903 1111 1 4.949333188700933 1111 2 9.034565682848712 1111 3 7.19486891843712 1111 4 7.636094603130154 1111 5 8.034828606323956 1111 6 7.166933954930229 1111 7 7.276736785272298 1111 8 7.477667902408694 1111 9 6.148138937595757 1111 10 5.39373860400611 1111 11 7.158412966721773 1111 12 6.365347958002349 1111 13 7.796651351195379 1111 14 5.949375247792123 1111 15 5.466349425513844 1111 16 5.388576555464574 1111 17 6.627982702593005 1111 18 6.436368581880014 1111 19 5.3238949762752235 1111 20 4.966952611214925 1111 21 7.777559505288696 1111 22 7.049558549144269 1111 23 6.890918776119463 1111 24 6.589728984309886 1111 25 7.268345917000882 1112 1 -1.4611735452022714 1112 2 8.012647186651265 1112 3 6.318559548369677 1112 4 0.6950675035257845 1112 5 4.4461732345756 1112 6 3.5374677474399157 1112 7 6.217420527559572 1112 8 6.261629528384955 1112 9 5.1100083388215936 1112 10 4.000912209565424 1112 11 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3.203739581763706 2204 4 5.878720971477158 2204 5 1.1282064931500777 2204 6 4.085648137696779 2204 7 4.980526119886761 2204 8 4.924915766243153 2204 9 3.6265327813210924 2204 10 3.444508996236987 2204 11 5.051776269285174 2204 12 4.830710334036116 2204 13 2.97839271216446 2204 14 4.2968624651147955 2204 15 4.455801571974156 2204 16 4.496384283408023 2204 17 -1.1597948273476248 2204 18 2.7022697177367085 2204 19 3.561047956612203 2204 20 3.827981745424456 2204 21 5.091631603020417 2204 22 4.593963339645626 2204 23 3.3743098745718143 2204 24 4.9083264929746555 2204 25 5.103110509842583 2205 1 5.216470743616853 2205 2 9.285062766623895 2205 3 6.178001208999156 2205 4 6.795800518900492 2205 5 6.681117994295477 2205 6 6.448283371685267 2205 7 6.277432702557558 2205 8 6.501698352340522 2205 9 5.088578833066141 2205 10 4.674813441151997 2205 11 6.7265607852341205 2205 12 6.000157077832679 2205 13 8.510596208035253 2205 14 6.295724285816664 2205 15 5.141753720317663 2205 16 4.693047468962529 2205 17 5.39964135279752 2205 18 5.2501562655643035 2205 19 3.984959587697734 2205 20 4.537903035393079 2205 21 -1.0120130973472676 2205 22 2.8458252132860657 2205 23 4.0750812136289305 2205 24 5.890109608457742 2205 25 5.9968766320440565 2206 1 3.651196542738198 2206 2 7.9010253101779035 2206 3 5.410707427753891 2206 4 1.9031753484717697 2206 5 5.173027364087929 2206 6 5.203424638160044 2206 7 5.479376536825093 2206 8 5.16089771607329 2206 9 4.236648254365942 2206 10 3.3129932623912026 2206 11 3.079996817653452 2206 12 4.274509448685578 2206 13 7.437955699021465 2206 14 0.1116423285374637 2206 15 1.9329643323461418 2206 16 2.8280175492533957 2206 17 3.735125559735863 2206 18 -1.1762090578715387 2206 19 0.6655098831075215 2206 20 2.2107414310906996 2206 21 3.0042675798228586 2206 22 -1.8860004570669098 2206 23 -3.080354515806004 2206 24 1.1285488600862834 2206 25 2.6021968486779024 2207 1 1.3978634731492434 2207 2 -13.063260404107812 2207 3 -5.70626650111425 2207 4 -4.242391767879063 2207 5 1.1461007113997148 2207 6 1.434311412698161 2207 7 2.807614658437629 2207 8 3.0174471483635124 2207 9 0.8908595927367919 2207 10 0.5630377692551498 2207 11 -1.3929776255162274 2207 12 -1.967316123969404 2207 13 0.47717613657339086 2207 14 -4.991346554636962 2207 15 -1.3570374479284162 2207 16 -0.05459312448162024 2207 17 1.0102373214164437 2207 18 1.0168468823112349 2207 19 0.43741220537677705 2207 20 0.2871893877311251 2207 21 -3.826294789129154 2207 22 -4.055220499002267 2207 23 -1.303477315188843 2207 24 0.5718421702101111 2207 25 0.9604029253483901 2208 1 5.3643758024707715 2208 2 6.624334149598692 2208 3 6.5523312613957465 2208 4 8.34512642015875 2208 5 4.064382218862836 2208 6 6.676718208038167 2208 7 7.017503410280522 2208 8 7.016370320164587 2208 9 5.531243541010497 2208 10 5.117458546498664 2208 11 7.189487660626174 2208 12 6.911350391732533 2208 13 9.292795204382251 2208 14 4.097885645176099 2208 15 5.68396272552042 2208 16 5.989680895773347 2208 17 6.62855472315286 2208 18 1.5036732022087094 2208 19 4.043220814220739 2208 20 5.096647408294448 2208 21 7.672250167845899 2208 22 6.766334373464655 2208 23 2.9601673266874897 2208 24 4.324454743323614 2208 25 5.494338672190178 2209 1 2.842333514565709 2209 2 4.396993967911268 2209 3 4.751238587338758 2209 4 0.6007864140495869 2209 5 4.938314618825313 2209 6 4.9114386898441715 2209 7 5.39506643205463 2209 8 3.936404040812352 2209 9 3.7428754808617093 2209 10 2.5781879419081335 2209 11 -1.2827733785566444 2209 12 2.2700880561567693 2209 13 1.3979076586537325 2209 14 3.015541843690176 2209 15 3.5262357184564017 2209 16 2.856317176977573 2209 17 0.6375056069368839 2209 18 2.5970507748512084 2209 19 3.3915452443730594 2209 20 3.3826054763562787 2209 21 5.680499941157521 2209 22 3.9878516101994776 2209 23 -0.5445155621281756 2209 24 -3.2486050567095788 2209 25 -0.8038244905275818 2210 1 -2.524857985935501 2210 2 -13.063260404107812 2210 3 -7.474120081039045 2210 4 -2.6371305958022226 2210 5 -1.509584449626555 2210 6 -1.9658842745092677 2210 7 -1.6799988529326946 2210 8 -19.458667501318015 2210 9 -12.533315451388754 2210 10 -7.437624113946445 2210 11 -9.62711672970368 2210 12 -4.991492447096306 2210 13 -5.6826964748915785 2210 14 -4.521596215701603 2210 15 -6.202713783845333 2210 16 -5.5976359966070675 2210 17 -5.1564849818091965 2210 18 -5.309603505226175 2210 19 -10.065115101302169 2210 20 -7.378018577889321 2210 21 -5.635943575356865 2210 22 -9.964068630381286 2210 23 -8.108100905089847 2210 24 -10.56014629319766 2210 25 -8.286834431723207 2211 1 -0.26669150707040146 2211 2 4.43474119461321 2211 3 0.3581991238380162 2211 4 1.1586510921004667 2211 5 0.6772655884153025 2211 6 -0.41825876046267396 2211 7 -19.18521973646683 2211 8 -12.680090363028647 2211 9 -8.861010885402989 2211 10 -7.064977652802106 2211 11 -9.211996295930216 2211 12 -4.661269878163502 2211 13 -6.233615739479907 2211 14 -4.70450723628273 2211 15 -5.095162234723183 2211 16 -11.173148598573686 2211 17 -7.481576234108562 2211 18 -5.566479450253286 2211 19 -8.525616098095096 2211 20 -6.537018125239392 2211 21 -4.446094490769113 2211 22 -5.983247504149741 2211 23 -8.040835199581965 2211 24 -20.489463562273965 2211 25 -20.23864166063887 2212 1 -0.9404455108603749 2212 2 -13.063260404107812 2212 3 -7.301824605430317 2212 4 -18.383315334758045 2212 5 -18.551351517899647 2212 6 -19.452631262461075 2212 7 -9.146758198005292 2212 8 -8.844157011807525 2212 9 -4.639573350116532 2212 10 -5.044741394256498 2212 11 -5.226604190034514 2212 12 -2.4145623490818693 2212 13 -5.472731439926543 2212 14 -3.3893132376354913 2212 15 -3.4145794282410975 2212 16 -3.492363156996877 2212 17 -3.7745495477653286 2212 18 -2.723715898856643 2212 19 -3.2197244477789866 2212 20 -3.3752866712417493 2212 21 -1.262199648600891 2212 22 -2.613213461453828 2212 23 -2.5371640106589766 2212 24 -1.7342188070292082 2212 25 -2.2272780242752326 2213 1 -17.53048047589534 2213 2 -13.063260404107812 2213 3 -8.254587101475362 2213 4 -5.567064180556752 2213 5 -18.551351517899647 2213 6 -10.098060625341962 2213 7 -19.18521973646683 2213 8 -10.029304620431589 2213 9 -7.916908549543145 2213 10 -6.958288488897775 2213 11 -5.910148023855356 2213 12 -4.851998181947067 2213 13 -17.882696474891578 2213 14 -11.609144191538467 2213 15 -6.873783658913471 2213 16 -6.122946101291731 2213 17 -4.700707300446627 2213 18 -4.65078651983308 2213 19 -5.564337475563757 2213 20 -5.4393964995482555 2213 21 -3.6752164563545406 2213 22 -4.516418751085713 2213 23 -4.3260452586815 2213 24 -3.4682262584881833 2213 25 -4.2820396292445935 2214 1 -17.53048047589534 2214 2 -13.063260404107812 2214 3 -11.913541496786383 2214 4 -18.383315334758045 2214 5 -18.551351517899647 2214 6 -19.452631262461075 2214 7 -15.594779249119128 2214 8 -14.313400397288024 2214 9 -13.701924658601861 2214 10 -13.67976230118462 2214 11 -14.043578452885065 2214 12 -12.239430779620404 2214 13 -17.882696474891578 2214 14 -16.036707080905625 2214 15 -22.27821579187746 2214 16 -17.49735558724704 2214 17 -21.93801108622687 2214 18 -16.92993751229475 2214 19 -14.542240848903633 2214 20 -13.576128355330857 2214 21 -11.081931463178066 2214 22 -12.015816013540917 2214 23 -12.052898276393243 2214 24 -10.943071809696646 2214 25 -10.690375493816001 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 160360.0 3 4.0 4 773.0 5 2769.0 6 3548.0 7 3390.0 8 2911.0 9 2799.0 10 2557.0 11 2533.0 12 2389.0 13 2528.0 14 3813.0 15 7592.0 16 10291.0 17 11379.0 18 11982.0 19 14165.0 20 16964.0 21 19159.0 22 19894.0 23 21503.0 24 22135.0 25 22239.0 26 18687.0 27 15629.0 28 17032.0 29 19072.0 30 18820.0 31 21741.0 32 27351.0 33 32979.0 34 39643.0 35 43165.0 36 41065.0 37 39760.0 38 24774.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05138081890507 18.140073616210145 12.367238092823609 26.441307472061176 2 13.271193771626297 20.221020761245676 42.106617647058826 24.401167820069205 3 20.441562564161554 23.612035361117623 27.782445543675 28.16395653104582 4 11.377388269288538 16.189132013815048 37.1750674302135 35.25841228668291 5 12.381510456060344 37.43672463292724 35.43595712800294 14.745807783009479 6 32.038430822100146 36.616248396699014 18.245444205125725 13.09987657607512 7 28.022311235365642 30.07983892731413 21.151402866681423 20.7464469706388 8 25.431769405031595 38.74061046858233 19.12990342196256 16.697716704423513 9 26.385238072956373 15.634728182035875 19.492834002636844 38.487199742370905 10 14.759270132648636 29.650338141747568 33.96731133726728 21.623080388336515 11 33.84753616603611 21.768065543022075 24.499267504885726 19.885130786056088 12 24.818598385500117 24.08045382113371 31.409705952247307 19.691241841118863 13 29.065270208155493 21.106769978330817 24.569407052334363 25.25855276117933 14 20.031006669227278 23.069380914138062 27.02510562519641 29.87450679143825 15 22.50747943951327 32.656983195885495 22.635880618083025 22.19965674651822 16 21.091995948750274 28.176300189105536 29.31532463117337 21.416379230970815 17 21.075277043434404 28.745222045602013 29.345919648195217 20.833581262768366 18 21.039286172807806 27.15004995897224 31.802514136435438 20.00814973178451 19 23.213643121950675 26.46388511525018 26.76459840270475 23.557873360094398 20 23.329574093784295 27.861341965797354 28.36925291318087 20.43983102723748 21 21.514458309145144 26.11936223654035 26.449997988309203 25.916181466005302 22 22.372619473378336 29.06198310161384 27.490053198623094 21.07534422638473 23 21.553197638412946 28.43537976686897 28.36051360389745 21.65090899082064 24 22.92765248978356 27.164068412289993 28.810806720145298 21.09747237778114 25 22.241306226706964 27.637583744832178 28.57467259004806 21.5464374384128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 735.0 1 735.0 2 1530.0 3 2325.0 4 2325.0 5 2325.0 6 3417.5 7 4510.0 8 4510.0 9 4510.0 10 5097.0 11 5684.0 12 5684.0 13 5684.0 14 6796.0 15 7908.0 16 7908.0 17 7908.0 18 11271.5 19 14635.0 20 14635.0 21 14635.0 22 20584.5 23 26534.0 24 26534.0 25 26534.0 26 35365.0 27 44196.0 28 44196.0 29 44196.0 30 54283.5 31 64371.0 32 64371.0 33 64371.0 34 71698.5 35 79026.0 36 79026.0 37 79026.0 38 82820.0 39 86614.0 40 86614.0 41 86614.0 42 87946.0 43 89278.0 44 89278.0 45 89278.0 46 89128.0 47 88978.0 48 88978.0 49 88978.0 50 82139.0 51 75300.0 52 75300.0 53 75300.0 54 65089.0 55 54878.0 56 54878.0 57 54878.0 58 45924.5 59 36971.0 60 36971.0 61 36971.0 62 29957.5 63 22944.0 64 22944.0 65 22944.0 66 17759.5 67 12575.0 68 12575.0 69 12575.0 70 9248.0 71 5921.0 72 5921.0 73 5921.0 74 4217.5 75 2514.0 76 2514.0 77 2514.0 78 1746.0 79 978.0 80 978.0 81 978.0 82 676.0 83 374.0 84 374.0 85 374.0 86 237.5 87 101.0 88 101.0 89 101.0 90 68.0 91 35.0 92 35.0 93 35.0 94 22.5 95 10.0 96 10.0 97 10.0 98 5.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.23682455887104 2 36.43068759064882 3 5.588710398064325 4 13.503804672839378 5 13.66327786140955 6 14.789763471016434 7 9.323270025227009 8 7.75850810082555 9 0.10571972587108792 10 1.4281098990232266 11 5.595446765512548 12 5.703366121570811 13 16.25457969878814 14 1.5699860460960002 15 2.996171268705449 16 2.9475044508142068 17 2.962489431464335 18 1.4857127145498663 19 1.4342963589246558 20 0.28471463235243577 21 4.325435286192509 22 1.5929446861746368 23 0.10516981832429423 24 9.169020958351377 25 0.10764440228486585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 727395.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.33939054028643 #Duplication Level Percentage of deduplicated Percentage of total 1 96.88590428538578 88.49499449371663 2 2.234097340778421 4.081221790287512 3 0.3800879619322518 1.0415100838377442 4 0.15288623872540488 0.5585814346870088 5 0.09064203943039442 0.4139594319450419 6 0.04760983436249884 0.2609191952636779 7 0.0355975828595312 0.22760230661678488 8 0.02723348706524192 0.1989992088660777 9 0.02090805407679162 0.17187560250817482 >10 0.10635022067228461 1.8252747424580889 >50 0.009341792844277786 0.5776591629215488 >100 0.008251359651756017 1.3654300553988938 >500 9.341161846501682E-4 0.6080586240449851 >1k 1.5568603077502805E-4 0.17391386744785237 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1223 0.16813423243217235 No Hit CTGTAGGACGTGGAATATGGCAAGA 937 0.1288158428364231 No Hit GTCCTAAAGTGTGTATTTCTCATTT 755 0.10379504945730998 No Hit GTCCTACAGTGTGCATTTCTCATTT 747 0.10269523436372259 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 1.3747688669842382E-4 0.0 12 0.0 0.0 0.0 1.3747688669842382E-4 0.0 13 0.0 0.0 0.0 1.3747688669842382E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTAGG 45 0.001212013 17.762245 1 TAGGACC 325 0.0 16.472815 4 GTCCTAC 720 0.0 16.282059 1 TGTAGGA 655 0.0 16.27535 2 CTGTAGG 635 0.0 15.314691 1 TCCTACA 760 0.0 14.925936 2 TTAAGCT 45 0.005208306 14.27644 4 GTAGGAC 690 0.0 14.107086 3 TTAGGAC 440 0.0 13.671192 3 TTTAGGA 420 0.0 13.667122 2 CCTACAG 910 0.0 13.460866 3 GTATTAG 60 0.008233141 13.321684 1 GTATAGC 90 4.8240065E-5 13.321684 1 GTCTTAG 60 0.008233141 13.321684 1 CAGGACC 105 5.6905265E-8 13.256693 4 CTTAGGG 50 0.009089783 13.124345 3 TGTAGAA 230 0.0 13.0729 2 AGGACCT 530 0.0 12.9902115 5 ATACACT 235 0.0 12.7576685 4 GACAGTG 80 1.3707433E-5 12.756741 7 >>END_MODULE