FastQCFastQC Report
Thu 26 May 2016
SRR1926543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926543_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727395
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT27480.37778648464726866No Hit
CTGTAGGACGTGGAATATGGCAAGA27010.3713250709724428No Hit
CCCATGTACTCTGCGTTGATACCAC18880.2595563620866242No Hit
GTACATGGGAAGCAGTGGTATCAAC18400.25295747152509984No Hit
GTCCTACAGTGTGCATTTCTCATTT18330.2519951333182109No Hit
GTCCTAAAGTGTGTATTTCTCATTT17440.23975969040205117No Hit
CTTTAGGACGTGAAATATGGCGAGG16900.2323359385203363No Hit
CTGTAGGACCTGGAATATGGCGAGA13830.19013053430392016No Hit
GTATCAACGCAGAGTACTTTTTTTT12250.1684091862055692No Hit
CTGAAGGACCTGGAATATGGCGAGA10650.14641288433382138No Hit
GAGTACATGGGAAGCAGTGGTATCA8090.11121880133902488No Hit
GATATACACTGTTCTACAAATCCCG7870.10819430983165955No Hit
GGTATCAACGCAGAGTACTTTTTTT7420.10200784993023047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA851.7716957E-814.49130119
TAGGACC5400.014.1225894
GGCGAGG3350.013.57623519
ACTGTTC2750.013.4772258
TTAGACT655.2716365E-513.1991894
GATATAC2750.013.1734351
TATGTCG802.0549414E-613.02823616
TGTAGGA14150.012.87643052
CTTAGAC603.9564882E-412.7156073
CTAGCAC1051.8824721E-812.7156063
GTTCCTA603.9680547E-412.711211
TAGCACC751.424684E-512.7103314
CTGTAGG14500.012.492051
GTAGGAC13850.012.4631323
CTGTTCT2850.012.337459
ACGAAAT1001.4750913E-712.32607712
TATAGTA701.0481109E-412.2648722
GAGTCCA701.0859654E-412.2183639
CGAAATC1103.9048246E-812.06748913
TCCAACG1103.9424776E-812.05919518
Produced by FastQC (version 0.11.5)